An experiment was carried out to explore the regression coefficients (RC) of semen characteristics on seminal proteins of buffalo bulls. SDS-PAGE resolved seminal proteins. A life sperm (LS) percentage exhibited a negative and significant RC on PF1 (111.5 ± 18.0 kDa), PF4 (36.13 ± 3.33 kDa), and PF5 (28. 88 ± 3.26 kDa) proteins, namely -0.13, -0.63 and -0.69 % / kDa respectively. A sperm acrosome integrity (SAI) percentage exhibited a pronounced negative RC on PF1 and PF2 (87.63 ± 16.5 kDa), -0.13 and -0.14 % / kDa, respectively. A negative RC was noticed in SAI percentage on PF4 (-0.61 % / kDa) and PF5 (-0.60 % / kDa) proteins. A pronounced positive RC was shown for total sperm abnormalities (TSA) on PF1 proteins (0.02 % / kDa). In conclusion, some semen attributes had a negative RC on some seminal proteins. In conclusion, some semen attributes had a negative RC on seminal proteins. These provide us with a good tool for early predicting the buffalo bull’s fertility to reduce the economic losses of male infertility and enhance the developmental sustainability of buffalo production in Iraq.