The running velocity associated with maximal oxygen uptake (vV[Combining Dot Above]O2max) has been used extensively to evaluate fitness level, training adaptations and to estimate performance level in running distances ranging from 1500 m to Marathon. However, the systematic use of vV[Combining Dot Above]O2max could be limited as for its determination subjects need to execute an exhaustive progressively incremental test in a sophisticated laboratory setting with the help of skilled laboratory personnel. Consequently, there is a need for a simple, inexpensive and valid field test that could provide a reasonable estimation of vVO2max. PURPOSE: The purpose of this study was to examine the relationship of the percentage of maximal heart rate (%HR) at submaximal running speed of 10, 11 and 12 kmh-1 with maximal oxygen uptake (V[Combining Dot Above]O2max), vV[Combining Dot Above]O2max and ventilatory threshold (VT). METHODS: Sixty trained amateur runners (age 38.2 ± 8.8 years, body mass 76.0 ± 13.0 kg, %fat 13.1 ± 4.3 % and V[Combining Dot Above]O2max 52.7 ± 7.8 mlkg-1 min-1) performed a laboratory based incremental treadmill test to volitional exhaustion (0% inclination, 3 min stages) to determine V[Combining Dot Above]O2max. Additionally, the subjects visited the laboratory three times (with interval of 48 hours) to run a 6-min running bout at submaximal speeds 10, 11 and 12 kmh-1 in a random order. Heart rate was collected throughout the submaximal running bouts. Data were assessed for normality (Kolomonov and Smirnoff) and the relationships between the variables were calculated using the Pearson’s product moment correlation coefficient. Significance was set at p < 0.01. RESULTS: The results revealed significant (p < 0.01) correlations between the %HR at all speeds and the vV[Combining Dot Above]O2max, V[Combining Dot Above]O2max, and VT (10 kmh-1 = -0.817, -0,708, and -0.810 respectively; 11 kmh-1 = -0.863, -0,728, and -0.858 respectively; 12 kmh-1 = -0.911, -0,794, and -0.904 respectively). The highest correlation occurred between the %HR at 12 kmh-1 and vV[Combining Dot Above]O2max (r = -0.911) and the equation for prediction of vV[Combining Dot Above]O2max was y = -0.2917x + 41.3 (r2 = 0.830). CONCLUSIONS: It can be concluded that the %HR can accurately predict vV[Combining Dot Above]O2max, V[Combining Dot Above]O2max, and VT and this can provide useful information regarding aerobic fitness of trained adults. The %HR at submaximal speeds could be used in monitoring athletes as well as for the estimation of the optimal training intensity.