Percent Contribution Research Articles

Clinical criteria accurately diagnose severe but not moderate alcohol-associated hepatitis: A systematic review and meta-analysis.

The precision of clinical criteria and the utility of liver biopsy for diagnosis or prognosis remain unclear in patients with alcohol-associated hepatitis (AH). We systematically reviewed the literature to answer these questions. Four databases were searched for studies describing the precision of clinical criteria (National Institute on Alcohol Abuse and Alcoholism, European Association for Study of Liver, or classical) and the role of histology in AH. The precision(positive predictive value) of criteria was pooled through random-effects meta-analysis, and its variation was investigated through subgroups and meta-regression of study-level factors with their percent contribution to variation (R2). The risk of bias among studies was evaluated through the QUADAS2 tool (PROSPERO-ID-CRD4203457250). Of 4320 studies, 18 in the systematic review and 15 (10/5: low/high risk of bias, N=1639) were included in the meta-analysis. The pooled precision of clinical criteria was 80.2% (95% CI: 69.7-89.7, I2:93%, p < 0.01), higher in studies with severe AH (mean-Model for End-Stage Liver Disease > 20) versus moderate AH (mean-Model for End-Stage Liver Disease < 20): 92% versus 67.1%, p < 0.01, and in studies with serum bilirubin cutoff 5 versus 3 mg/dL (88.5% vs.78.8%, p = 0.01). The factors contributing to variation in precision were Model for End-Stage Liver Disease (R2:72.7%), upper gastrointestinal bleed (R2:56.3%), aspartate aminotransferase:aspartate aminotransferase ratio (R2:100%), clinical criteria (R2:40.9%), bilirubin (R2:22.5%), and Mallory body on histology (R2:19.1%).The net inter-pathologist agreement for histologic findings of AH was variable (0.33-0.97), best among 2 studies describing AH through simple and uniform criteria, including steatosis, ballooning, and neutrophilic inflammation. Few studies reported the utility of histology in estimating steroid responsiveness (N = 1) and patient prognosis (N = 4); however, very broad septa, pericellular fibrosis, and cholestasis were associated with mortality. Bilirubinostasis was associated with infection in 1 study. Clinical criteria are reasonably precise for diagnosing severe AH, while there is an unmet need for better criteria for diagnosing moderate AH. Histologic diagnosis of AH should be simple and uniform.

Intake of free sugars by 11–13-year-old schoolchildren living in Delhi, India

Intake of free sugars is associated with a risk of non-communicable diseases including dental caries, and authoritative organisations recommend limiting intake to &lt;5% energy intake (E) or lower (1, 2). National surveys of schoolchildren in India indicate the prevalence of obesity is rising &gt;10%/year(3) and that 52.5% of young adolescents are affected with dental caries(4), yet, there is a dearth of data on dietary intake of sugars by this population. The objective of this research was to assess the intake of total and free sugars, and the contribution of food sources to free sugars intake, in a random sample of 11–13-year-old schoolchildren in Delhi, India. The study was approved by The University of Adelaide Human Research Ethics Committee and the Independent Ethics Committee of the Centre for Chronic Disease Control, New Delhi. The target sample size of 360 was based on a ± 5% margin of error in estimated sugars intake. A statistician external to the research team generated a random sample of 150 schools stratified by district (n = 11). Schools were recruited in turn from the list until 10 schools had consented. Teachers shared study information with parents who were invited to complete an online consent form. Child assent was obtained before data collection. Participants recorded all food and drink consumed over three consecutive days, including one weekend day, in a food diary. The information recorded was entered into an online dietary assessment tool, Intake24 (Southeast Asia version), during an interview with each participant during which portion size was ascertained with reference to the database of over 2400 food photographs of more than 100 foods. The Intake24 database converted food and drink reported into the intake of total and free sugars through integrated food compositional tables. Of 514 pupils providing consent, 393 participants (76.5%) (169 girls, 224 boys) completed the study. In girls, the median (IQR) daily intakes of total and free sugars were 95.0 (70.1-120.2) g/day and 43.0 (28.1-68.5) g/day respectively. The corresponding values in boys were significantly higher at 104.0 (80.2-138.7) g/day and 53.1 (34.1-76.5) g/day (p = 0.004). No between-gender difference was observed in the median percent contribution of sugars to E: total and free sugars contributed 14.9% (IQR 11.4-18.1%) and 7.1% (IQR 4.8-10.1%) respectively. The percent contribution of the main sources of sugars to free sugars intake were: (i) Sugars Preserves and Syrups (31.2% (IQR. 9.6-51.7%)); (ii) Cakes and Biscuits (13.7% (IQR 0-26.4%)); (iii) Desserts (5.4% (0-17.5%)) and (iv) Sugar-Sweetened Beverages and Juices (2.1% (IQR 0-15.7%)). In conclusion, in this sample of 11-13-year-old schoolchildren from Delhi, free sugars intake was above the WHO &lt;5% E threshold. Forms of sugars that are added to foods by the consumer made the largest contribution to intake.

Exploring multivariate associations of yield and yield-associated traits in okra (Abelmoschus esculentus (L.) Moench) accessions in the Northwestern Region of India

Evaluating genetic diversity simplifies the identification of superior genotypes, facilitating the development of high-yielding, resilient to climatic conditions and promoting effective crop improvement. The present study aimed to examine the divergence, correlation and path analysis across 55 okra (Abelmoschus esculentus (L.) germplasms for 17 traits during the summer season (March- July) of 2022. The experiment utilized randomized complete block design with three replications conducted in Phagwara, Punjab. Analysis of variance suggested a sufficient amount of genetic variation was found among all genotypes. Using Mahalanobis D2 analysis, the samples were classified into seven clusters, the largest being cluster I, composing 34 germplasm. Only one germplasm was found in Clusters II, IV, V, VI, and VII. The inter-cluster distance was highest between clusters II and VII, whereas the intra-cluster distance was greatest in Cluster III. The number of fruits per plant had the highest percent contribution to the divergence, accounting for 49.63%. At both phenotypic and genotypic levels, there was a strong positive correlation (+) observed between fruit yield and various characteristics, including plant height, fruit length, number of fruits per plant, number of marketable fruits per plant, average fruit weight, and the number of pickings. Genotypic path analysis revealed that characteristics such as the first flowering node, days to first flowering, days to 50% flowering, plant height, inter-nodal length, number of nodes per plant, number of fruits per plant, marketable fruits per plant, and average fruit weight exhibited a positive and direct effect on fruit yield. When selecting this trait to improve yield in okra through breeding, it is essential to focus on specific characteristics that directly contribute to higher production. This research will help resilient okra varieties understand yield-influencing factors in Punjab environmental conditions.

A Global Climatology of Diabatic Heating in Tropical Easterly Waves

Abstract Tropical easterly waves (TEWs) play a critical role in regulating convection and precipitation across the global tropics. TEWs act as seed disturbances for tropical cyclogenesis, serve as an essential component in monsoon precipitation, and produce large amounts of rainfall and diabatic heating that can strongly affect the large-scale circulation. To help improve our knowledge of a more elusive type of tropical wave, we use satellite and reanalysis estimates of the diabatic heating associated with TEWs that are identified by a tracking algorithm based on low-level curvature vorticity. This study uses the Tropical Rainfall Measuring Mission (TRMM) version 6 convective–stratiform heating (CSH) and spectral latent heating (SLH) orbital products to create a global climatology (1998–2015) of TEW diabatic heating. TEW-specific composites for the satellite-observed vertical structure of diabatic heating are compared to similar terms from MERRA-2 across a variety of tropical regions. There are striking differences between the reanalysis and satellite heating with MERRA-2 having much stronger background heating, especially at low levels. Both the satellite-observed and reanalysis heating profiles show stronger midlevel heating associated with TEWs relative to the unconditional background. Similar patterns of mid- and bottom-heaviness emerge in two-dimensional composites of TEW latent heating as stronger heating rates and percent contributions to the background are generally higher at 500 hPa than at 850 hPa. Although TEWs only represent a few percent of the background heating across the global tropics, they comprise 30%–50% of the heating in the prominent TEW tracks over the northeastern Atlantic and Pacific Oceans.

Citizen-science data identifies the daily movement patterns and habitat associations of a nocturnal urban-invading bird species (Corvus frugilegus)

Rooks (Corvus frugilegus) are an invasive bird species in South Korea that are deemed harmful due to nocturnal urban invasions and agricultural damage. Employing citizen science data, we document the daily movement patterns and habitat associations of nocturnal urban-invading rooks in Suwon, South Korea. We collected 4,522 geolocated observations from December 2021 to March 2022, categorized by the timings of sunrise and sunset and segmented into 3-h intervals. Using the MaxENT (Maximum Entropy Model) and k-fold cross-validation (k = 10) with a 25% random test sample, we analyzed daily movement patterns and habitat preferences. Our results show that rooks forage in agricultural areas shortly after sunrise and roost in urban environments after sunset. Urban roosts tend to occur in areas near buildings of medium height (6–20 stories) that are illuminated by artificial light and contain utility poles. The variables with the highest percent contribution to rooks’ presence after sunset were buildings of medium height (20.7%), land use type (19.4%), elevation (29%), and utility pole (14.5%). Our findings emphasize the potential of citizen science initiatives to generate insights into the ecology of invasive bird species within large urban environments.

Analisis Pengaruh Parameter Terhadap MRR Pada Benda Kerja di Mesin CNC Turning dengan Metode Taguchi

A high material removal rate is one of the goals to be achieved when machining parts with CNC turning. However, slow processing affects product processing time and increases production costs. To overcome this problem, it is necessary to set the correct process parameters so that they provide the most influential contribution to the MRR and produce maximum parameter values. This research aims to determine the influence of parameters (spindle speed, feed speed, and feed depth) on MRR and to determine the correct process parameter settings to produce maximum MRR, namely in this experimental design using an orthogonal array L9 (33) with three replications. The material used is ST-60 steel, with the data analyzed using the Taguchi Larger Is a Better method in analysis software. From the results of the mean factor calculation, the percent contribution to feeding was found to be 19.53%, while the depth of feeding was found to be 64.56%. The optimum MRR value is obtained from a combination of parameters in the CNC Turning process on ST-60 steel which can make the response value optimally obtained at the spindle speed 3,184 [rev/min], feeding 0.22 [mm/rev] and depth of cut 2.5 [mm].

Soil Quality Assessment of Sesame-Growing Soils with Different Productivity in the Northern Telangana Zone, India

Present study was carried out in major sesame growing areas of Northern Telangana zone in districts namely; Nizamabad, Jagtial, Nirmal and Kamareddy. The main objective of this study is to assess the soil quality and to find out the relation between sesame yield and soil quality index. Based on the seed yield data of sesame, the above-mentioned districts were divided into high, medium and low productivity zones. 50 samples from each productivity zone with a total of 150 surface soil samples (0-15 cm were collected and analysed for various physical, physico-chemical, chemical and biological properties. Physical properties included texture, bulk density and water holding capacity. Physico-chemical properties like pH, EC and organic carbon were analysed. Chemical properties of soil namely available nitrogen, available phosphorus, available potassium, available sulphur, exchangeable bases like exchangeable calcium and magnesium, potassium and sodium, available micronutrients like iron, copper, manganese and zinc were analysed. Biological properties like urease, acid and alkaline phosphatase and labile carbon were analysed. Principal component analysis, minimum dataset was derived which contained available nitrogen, sand, pH, available phosphorus, exchangeable calcium and magnesium and urease with 70.81% variance. These are identified as the key indicators of soil quality. Mean soil quality index values were 0.669, 0.549 and 0.443 for high, medium and low sesame productivity zones respectively and is in the order of high&gt;medium&gt;low sesame productivity zones. Percent contribution of MDS to SQI are in the order of, available nitrogen (44.44%&gt; pH (16.85% &gt;exchangeable calcium (9.87%&gt; exchangeable magnesium (8.89%&gt; urease (7.86%&gt; available phosphorus (7.38% &gt; sand (4.89%. This study concluded that the SQI was more significantly positively correlated with sesame yield, which revealed that soil variables from the minimum data set had biological significance and effectively evaluated the status of the sesame growing soils of Northern Telangana Zone.

Pengaruh Perubahan Kadar pH Air Dengan Optimalisai Ukuran dan Suhu Aktivasi Serbuk Cangkang Siput Hisap (Cerithidea Obtusa) Sebaga Media Adsorben

The demand for clean water continues to increase along with population growth. Therefore, it is important to improve water quality to meet the demand for clean water supply. There are three methods that can be used in the water purification process, namely filtration, sedimentation, and adsorption. Clam shell waste contains calcium carbonate (CaCO3) with a percentage of 98%. This content can be used as an adsorption media. The purpose of this research is to utilize gold snail shell waste for activation at mesh size and temperature by determining the most optimum value. The results of making adsorbents will be tested on borehole water at the Bangka Belitung State Manufacturing Polytechnic to study changes in the degree of acidity of water pH. The research was conducted using the Taguchi method, where the process parameters were mesh size and activation temperature. The parameters were then adjusted with the OA L_9 algorithm for three repetitions. Based on the results of the research and test data processing that has been done, it is concluded that the activation temperature is the highest influence in the process of making adsorbents. Optimal results were obtained at a powder size of 150 mesh (109 μm) and at an activation temperature of 700o C. With a percent contribution value on mesh size of 3.42% and activation temperature of 94.32%. It can be concluded that activation temperature is the highest influence in the process of making adsorbents.

Assessment of wild prey of mammalian carnivores in Barda Wildlife Sanctuary, Gujarat, India

The Barda Wildlife Sanctuary (Barda WLS) is a semi-arid ecosystem that plays an important role in the conservation of mammalian carnivores. It is essential to evaluate the population of important wild prey species in Barda, which has conservation implications for mammalian predators and themselves. We assessed the density, biomass, and population structure of wild prey species by surveying road (n = 10) and trail (n = 8) transects using the distance sampling framework method. We used male-to-female sex ratios and the percent contribution of each age class to assess population structure. Mean group size (MGS), median group size (Mdgs), and mean crowding (Mc) were calculated to analyze group size. Of the species studied, Indian peafowl had the highest density (51.61 ± 7.08 individuals/km2), followed by wild pig (10.77 ± 4.59), blue bull (3.50 ± 0.51), Indian hare (3.03 ± 0.73), and spotted deer (0.62 ± 0.04). The male-to-adult female sex ratio was biased towards females, except for Indian peafowl. All species had small group sizes except wild pigs. The results of this study can aid in its development as one of the important protected areas in Gujarat for the conservation of mammalian predators, along with framing conservation strategies for Barda WLS.

Food sources of fiber and micronutrients of concern among infants and young children in Lebanon: a national cross-sectional study

BackgroundIntakes of fiber, iron, zinc, calcium, vitamin D, vitamin A, and folate were shown to be low in a substantial proportion of infants and children in Lebanon. The study aims to identify the top food sources of fiber, iron, zinc, calcium, vitamin D, vitamin A, and folate amongst infants and young children in Lebanon and to evaluate the evolution of food sources of these nutrients from the beginning of the complementary feeding journey up until the age of 47.9 months.MethodsA national cross-sectional survey was conducted in 2012 as part of the “Early Life Nutrition and Health in Lebanon” project using stratified cluster sampling. Dietary intakes for infants and young children aged 6-47.9 months (n = 763) were assessed using 24- Hour Dietary Recall. Food items were categorized into food groups and the percent contribution of each food group to nutrient intakes was determined to identify the top food sources of fiber and selected micronutrients for three age groups: 6-11.9 m (infants), 12-23.9 m (toddlers), and 24-47.9 m (preschoolers).ResultsThe top food source of fiber was vegetables among children aged 6-47.9 months. Among infants and toddlers, infant/young child formula was the main contributor to iron, zinc, calcium, vitamin D, vitamin A, and folate intakes. Baby cereals also contributed to around 14% of iron intakes among infants. Among preschoolers, meat and fish contributed to 13% of iron intakes and 29% of zinc intakes, while cow’s milk was the major contributor of calcium (41%), vitamin D (81%) and vitamin A (25%) intakes. Sweetened beverages and sweet bakery were also ranked among the major food sources contributing to substantial intakes of key nutrients, including fiber, iron, zinc, calcium, vitamin A, and folate among infants, toddlers, and preschoolers.ConclusionsIn addition to milk sources, vegetables, beans and legumes, breads, meats, and rice and pasta, sweet bakery and sweetened beverages have contributed to intakes of key nutrients from early ages. This calls for implementing initiatives and designing approaches to support nutrition education and improve nutrient intakes in infancy and early childhood.

Lower extremity and trunk sagittal plane coordination strategies and kinetic distribution during landing in males and females

ABSTRACT Force attenuation during landing requires coordinated motion of the ankle, knee, hip, and trunk, and strategies may differ between sexes. Sagittal plane coordination of the ankle/knee, knee/hip, and knee/trunk, and lower extremity and trunk kinematics and kinetics was compared throughout landing between 28 males and 28 females. Coordination was assessed with a modified vector coding technique and binning analysis. Total support moments (TSM), each joint’s percent contribution, and timing of the TSM were compared. Females landed with less isolated knee flexion in the ankle/knee, knee/hip, and knee/trunk couplings, but more simultaneous ankle/knee flexion, less simultaneous knee flexion/hip extension, and more simultaneous trunk/knee flexion. Females landed with larger plantarflexion angles from 0–16% and smaller trunk flexion angles from 0–78%. In females, absolute TSM were larger from 0–6% and smaller from 42–100%, and normalized TSM were larger from 0–8% and 26–42%. Females had greater ankle contribution to the TSM from 14–15% and 29–35%, smaller absolute peak TSM, and the peak TSM occurred earlier. Females compensated for less isolated knee flexion with greater simultaneous ankle/knee flexion early in landing and knee/trunk flexion later in landing. Coordination and TSM differences may influence force attenuation strategies and have implications for knee injury disparity between sexes.

Influence of Integrated Nutrient Management (INM) Practices on Soil Quality Indicators and Indices Under Cotton (Gossypium Spp.) + Black Gram (Vigna Mungo (L.) Hepper) and Green Gram (Vigna Radiata (L.) R. Wilczek) + Rabi Sorghum (Sorghum Bicolor (L.) Moench) Inter Cropping Systems in Rainfed Vertisols of Western India

ABSTRACT A field experiment was conducted to study the effect of seven predominant integrated nutrient management (INM) practices on soil quality indicators and indices under two inter-cropping systems, namely cotton + black gram and green gram + rabi sorghum, at the All-India Coordinated Research Project for Dryland Agriculture (AICRPDA), Parbhani Center, located in Central and Western Maharashtra, India. The INM practices include F1: Farmyard Manure (FYM) @ 5 t ha−1; F2: Gliricidia @ 3t ha−1 (semi dried leaves + very soft upper twigs); F3: Recommended dose of fertilizer (RDF); F4: 25% RDF + FYM @ 2.5 t ha−1; F5: 25% RDF + Gliricidia @ 1.5 t ha−1 (semi dried leaves + very soft upper twigs); F6: Control with rotation and F7: Absolute control without rotation. The order of overall performance of the INM treatments, in terms of mean Soil Quality Index (SQI), across both the cropping systems studied was: F1 (2.59) > F4 (2.55) > F5 (2.52) > F2 (2.49) > F3 (2.40) > F6 (2.12) > F7 (2.04). In case of Cotton + black gram system, the predominant soil parameters which emerged as key soil quality indicator, along with their percent contribution toward soil quality index, were: organic C (21%), available Mn (21%), labile C (20%), dehydrogenase activity (DHA) (19%), pH (5%), available S (5%), mean weight diameter of soil aggregates (MWD) (5%) and available K (4%). However, in case of green gram + rabi sorghum system, labile C (24%), available K (23%), available Mn (23%), microbial biomass C (MBC) (21%) and available S (9%) were found to be the key soil quality indicators in the black soil under study. The common indicators for the two cropping systems studied under these Vertisol soils were: available K, available S, available Mn and Labile carbon (LC). Thus, these indicators assume great significance in managing the Vertisol soils under study. The methodology followed and the results of the present study will be immensely useful to different stakeholders in conducting such future studies and managing and improving soil quality under different inter-cropping systems in Vertisols.

Optimization on removal of 2,4-dichlorophenol by sodium carboxymethyl cellulose modified nanoscale Pd/Fe bimetal with response surface methodology

2,4-dichlorophenol (2,4-DCP) is a toxic and harmful organic intermediate widely used in industrial production. Recently, the utilization of nano-zero-valent iron (NZVI) for 2,4-DCP treatment has gained significant popularity, but the deactivation and agglomeration of NZVI always appear and continue to present challenges. To address these issues, sodium carboxymethyl cellulose (CMC) and noble metal catalyst Pd are used as stabilizers to improve the removal efficiency of NZVI on 2,4-DCP. The CMC-modified nanoscale Pd/Fe bimetal (CMC-Pd/Fe) particles were prepared by the liquid phase reduction method and characterized by scanning electron microscopy (SEM), Fourier infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The results showed that the addition of Pd and CMC succeeded in ameliorating the aggregation of NZVI. A five-level-four-factor central composite design (CCD) of response surface methodology (RSM) was used to optimize the removal of 2,4-DCP by CMC-Pd/Fe and explored the factors influencing the removal of 2,4-DCP, including the amount of catalyst, the initial pH of the system, the time of reaction, and the initial concentration of 2,4-DCP. A statistically significant and well-fitting quadratic regression model was successfully constructed to predict 2,4-DCP removal efficiency. The high F-value of 14.35, very low p-value (<0.0001), nonsignificant Lack of Fit, and appropriate coefficient of determination of 0.9399 demonstrate a good correlation between the experimental and predicted values of the proposed model. The analyses of variance reveal catalyst amount and reaction time have a positive effect on the removal efficiency of 2,4-DCP and the CMC-Pd/Fe catalyst amount shows the most apparent significance, with PC (percent contribution) greater than 26. Conversely, pH and 2,4-DCP concentration have a negative impact on the removal process. The optimum condition is 1.10 g/L of catalyst amount, 5.63 of pH, 2.31 h of reaction time, and 20 mg/L of initial 2,4-DCP concentration. The validation results from parallel experiments showed that the average removal efficiency of 2,4-DCP reached 96.32% under the optimal conditions.

Analyzing The Perspective Of Farmers On The Pradhan Mantri Fasal Bima Yojana (PMFBY) In The Sohawal Block Of The Satna District, In Madhya Pradesh, India.

Agriculture serves as the backbone of the Indian economy, despite its modest 16 percent contribution to the country's GDP. A staggering 60–72 percent of the population relies on agriculture for their livelihoods. However, this vital sector faces significant risks, such as droughts, floods, and other natural disasters, which often lead to losses. In recent years, issues such as indebtedness, crop failures, low prices, and poor returns have compounded the challenges, resulting in agrarian distress in various regions across the country. Despite the Indian government's implementation of numerous schemes aimed at agricultural development, the sector continues to grapple with uncertainties. Regrettably, even in the 21st century, farmers find themselves vulnerable to these challenges, highlighting the persistent issues within the agricultural landscape. Farmers who experience crop loss or damage can receive financial assistance through the PMFBY, a crop-based insurance scheme. During the 2021–22 period, a research study in the Sohawal block of Satna district aimed to gain insights into farmers' perspectives on the PMFBY. The study identified several factors that were significantly associated with respondents' perceptions of the PMFBY, including education level, land holding size, farming experience, annual income, extension contact, risk orientation, awareness of crop insurance, information sources, and exposure to mass media, achievement motivation, and economic motivation. These findings were statistically significant at a 0.05% level of significance. However, other profile characteristics, such as age, caste, and social participation, did not show any significant association with the respondents' perceptions of the PMFBY. This study sheds light on the multifaceted influences that shape farmers' perspectives on this important government scheme.

Lower-Extremity Energetic Distribution During Rate-Controlled Ballet Jumps (Sautés) in Healthy Dancers.

Dancers frequently perform jumps in the context of a prolonged, continuous dance piece. The purpose of this study is to explore the lower-extremity energetics in healthy dancers performing repetitive dance jumps (sautés) before and after typical dance-specific choreography. Lower-extremity kinetic data were collected from 14 healthy female dancers during a series of sautés performed before and after 3minutes of dance. Percent contributions of the lower-extremity joints to the whole-limb mechanical energy expenditure during ground contact were calculated. The jumps performed at the beginning were compared with the jumps at the end of the dance choreography. Dancers maintained the jump rate and consistent whole-limb mechanical energy expenditure between the jump series. As expected, for both jump series, the sautés had greater relative energetic contribution from the ankle and knee as compared with lesser contribution from the hip and toe. However, we observed lesser contribution from the knee and greater contribution from the hip after a 3-minute dance. After performing typical dance choreography, the dancers demonstrated a distal to proximal redistribution of individual joints' contribution to whole-limb mechanical energy expenditure.

Increased CAR-T Infusions Occur in Conjunction with Decreased Hematopoietic Stem Cell Transplantation

Background: Chimeric Antigen Receptor T-cell (CAR-T) therapies were first approved by the FDA in 2017, and multiple commercial products are currently available. Hematopoietic stem cell transplantation (HSCT) is another treatment strategy often utilized for patients with advanced and/or relapsed hematologic malignancies. Both CAR-T and HSCT require significant, overlapping resources for their collection, storage, and processing. Understanding the impact of evolving standards of care in clinical practice is necessary in the planning of resource allocation and laboratory expansion. We aimed to characterize the trends of CAR-T and HSCT infusions over a five-year period. Methods: The processing lab database was reviewed to compile a list of patients that received either CAR-T or HSCT (autologous or allogeneic) infusions, between 01/01/2018 and 12/12/2022. The number of individual HSCTs and CAR-T infusions were plotted over time. We also calculated the percent contribution of both HSCT and CAR-T to the total number of products infused per year. We subsequently examined these parameters, within the specific diagnoses of diffuse large B-Cell lymphoma/large cell lymphoma (DLBCL/LCL, including double and triple hit DLBCL and High-Grade lymphoma) and multiple myeloma (MM), including MM with amyloid and MM with plasma cell leukemia/plasmacytoma. Simple linear regression was used to calculate statistical significance to determine if the slopes were non-zero, and the direction of change over time. Results: Overall, our study included 210 CAR-T infusions and 2366 HSCTs. 89/210 (42%) patients who received a CAR-T product also received HSCT between 2018 and 2022, with 85/89 receiving HSCT prior to CAR-T infusion and 4/89 receiving CAR-T prior to HSCT, including two patients who received HSCT before and after CAR-T. The median interval between the most recent HSCT and subsequent CAR-T was 37 months. DLBCL/LCL and MM were the disease indications for which CAR-T was most utilized, accounting for 87.6% of the 210 procedures. The total number of CAR-T procedures increased significantly over the five-year period, from 24 in 2018 to 74 in 2022 (Slope=13.8; p=0.02), accompanied by a marked decrease in the number of HSCT from 502 in 2018 to 393 in 2022 (Slope=-26.6; p=0.06). The overall number of infusions (calculated as number of HSCT + number of CAR-T) declined slightly, though this was not statistically significant (mean 511 + 30.6 per year; Slope=-12.8; p=0.22). When expressed as a percentage of the total number of infusions, we observed a significant increase in the proportion of CAR-T, from 4.6% in 2018 to 15.8 % in 2022, (Slope=2.98; p=0.02), and a significant decrease in the proportion of infusions that were HSCT, from 95.4% in 2018 to 84.2% in 2022 (Slope=-2.98; p=0.02). In DLBCL, we observed a slight decrease in the number of overall infusions, from 71 in 2018 to 58 in 2022 (Slope=-3.5; p=0.18) and a significant decrease in HSCT from 47 in 2018 to 26 in 2022 (Slope=-6.0; p=0.0172), while the number of CAR-T infusions remained stable (mean=27.4 + 5.3 per year; Slope=2.5; p=0.14). In MM, we observed a marked increase in CAR-T infusions, from none in 2018 to 33 in 2022 (p=0.0544), while the total number of infusions remained stable (Mean 231.8 + 16.54; slope=-3.5; p=0.58) and HSCT slightly decreased, from 228 in 2018 to 184 in 2022, though this was not statistically significant (Slope=-11.5; p=0.18). We observed a non-significant increase in the percent contribution of CAR-T to the total number of infusions performed for MM, from 0% in 2018 to 15.3% in 2022 (Slope=3.62; p=0.06) while in DLBCL we observed a significant increase, from 33.8% in 2018 to 55.2% in 2022 (slope=6.42; p=0.017). Conclusion: The findings from this study suggest a gradual trend in the clinical utilization of human cellular therapy products over time. Since many of the resources and personnel involved in the collection, storage, and processing of these products are shared, an increase in CAR-T is an important observation. These findings underscore key issues in lab management for cell manufacturing facilities when considering resource allocation and infrastructure development. More analysis is needed to determine if the decrease in HSCT and the increase in CART are related.

Neo-Natal Castration Leads to Subtle Differences in Porcine Anterior Cruciate Ligament Morphology and Function in Adolescence.

Female adolescent athletes are at a higher risk of tearing their anterior cruciate ligament (ACL) than male counterparts. While most work related to hormones has focused on the effects of estrogen to understand the increased risk of ACL injury, there are other understudied factors, including testosterone. The purpose of this study was to determine how surgical castration in the male porcine model influences ACL size and function across skeletal growth. Thirty-six male Yorkshire crossbreed pigs were raised to 3 (juvenile), 4.5 (early adolescent), and 6 months (adolescent) of age. Animals were either castrated (barrows) within 2 weeks after birth or were left intact (boars). Posteuthanasia, joint and ACL size were assessed via MRI, and biomechanics were assessed via a robotic testing system. Joint size increased throughout age, yet barrows had smaller joints than boars. ACL cross-sectional area (CSA), length, volume, and in situ stiffness increased with age, as did the percent contribution of the ACL anteromedial (AM) bundle to resisting loads. Boar ACL, AM bundle, and PL bundle volumes were 19%, 25%, and 15% larger than barrows across ages. However, ACL CSA, in situ stiffness, and bundle contribution were similar between boars and barrows. The barrows had smaller temporal increases in AM bundle function than boars, but these data were highly variable. Early and sustained loss in testosterone leads to subtle differences in ACL morphology but may not influence measures associated with increased injury risk, such as CSA or bundle forces in response to applied loads.

Variations in the Major Nutrient Composition of Dominant High-Yield Varieties (HYVs) in Parboiled and Polished Rice of Bangladesh.

Rice (Oryza sativa L.) is the principal staple food, a fundamental component of food security, a significant source of energy and major nutrients, and a key player in the overall nutritional status in Bangladesh. Parboiling is a common rice-processing treatment in Bangladesh. Recently, polishing has also become a common practice among millers seeking to attract consumers. Polishing may influence the nutrient composition of rice. The present study aimed to investigate the impact of parboiling and polishing on the nutritional content of the five High Yield Varieties (HYVs) of rice (BR11, BRRI dhan28, BRRI dhan29, BRRI dhan49, and BRRI dhan84) and their percent contributions to the Recommended Dietary Allowances (RDA) of vitamins and minerals. All of the rice samples were analyzed for proximate parameters, vitamins (B1, B2, B3, B6, and folate), and minerals (Ca, Mg, Fe, Zn, Na, K, P). Moisture, ash, fat, and total dietary fiber (TDF) were determined gravimetrically, according to the AOAC Official Methods; protein was measured by the Kjeldahl method; B-group vitamins were measured using Ultra Pressure Liquid Chromatography; and mineral content was determined by ICP-OES. The energy, protein, fat, and total dietary fiber (TDF) content of the samples ranged between 342-357 kcal/100 g, 6.79-10.74 g/100 g, 0.31-1.69 g/100 g, and 2.59-3.92 g/100 g respectively. Thiamin, riboflavin, niacin, pyridoxin, and folate content ranged from 0.11-0.25 mg/100 g, 0.01-0.05 mg/100 g, 2.82-6.42 mg/100 g, 0.12-0.30 g/100 g, and 5.40-23.95 g/100 g respectively. In a comparison of parboiling and polishing, macronutrients and vitamin retention were higher in parboiled unpolished rice than in polished unparboiled rice. The minerals (mg/100 g) Ca, Mg, Fe, Zn, Na, K, and P were in the ranges 32.82-44.72, 30.69-58.34, 0.51-0.70,1.83-2.79, 5.00-5.36, 106.49-112.73, and 162.23-298.03. Minerals of BRRI dhan84 were unaffected by polishing and parboiling. BRRI dhan84 contributed a higher percentage of RDA of all B vitamins and minerals. Therefore, to reduce nutrient loss in rice, industries and consumers should be encouraged to avoid polishing or limit polishing to 10% DOM and to consume unpolished rice, either parboiled or unparboiled.

A Novel Method for Deriving Adverse Event Prevalence in Randomized Controlled Trials: Potential for Improved Understanding of Benefit-Risk Ratio and Application to Drug Labels.

Adverse event (AE) data in randomized controlled trials (RCTs) allow quantification of a drug's safety risk relative to placebo and comparison across medications. The standard US label for Food and Drug Administration-approved drugs typically lists AEsby MedDRA Preferred Term that occur at ≥ 2% in drug and with greater incidence than in placebo. We suggest that the drug label can be more informative for both patients and physicians if it includes, in addition to AE incidence (percent of subjects who reported the AE out of the total subjects in treatment), the absolute prevalence (percent of subject-days spent with an AE out of the total subject-days spent in treatment) and expected duration (days required for AE incidence to be reduced by half). We also propose a new method to analyze AEs in RCTs using drug-placebo difference in AE prevalence to improve safety signal detection. AE data from six RCTs in schizophrenia were analyzed (five RCTs of the dopamine D2 receptor-based antipsychotic lurasidone and one RCT of the novel trace amine-associated receptor 1 [TAAR1] agonist ulotaront). We determined incidence, absolute prevalence, and expected duration of AEs for lurasidone and ulotaront vs respective placebo. We also calculated areas under the curve of drug-placebo difference in AE prevalence and mean percent contribution of each AE to this difference. A number of AEs with the same incidence had different absolute prevalence and expected duration. When accounting for these two parameters, AEs that did not appear in the 2% incidence tables of the drug label turned out to contribute substantially to drug tolerability.The percent contribution of a drug-related AE to the overall side effect burden increased the drug-placebo difference in AE prevalence, whereas the percent contribution of a placebo-related AE decreased such difference, revealing a continuum of risk between drug and placebo. AE prevalence curves for drug were generally greater than those for placebo. Ulotaront exhibited a small drug-placebo difference in AE prevalence curves due to a relatively low incidence and short duration of AEs in the ulotaront treatment arm as well as the emergence of disease-related AEs in the placebo arm. Reporting AE absolute prevalence and expected duration for each RCT and incorporating them in the drug label is possible, is clinically relevant, and allows standardized comparison of medications. Our new metric, the drug-placebo difference in AE prevalence, facilitates signal detection in RCTs. We piloted this metric in RCTs of several neuropsychiatric indications and drugs, offering a new way to compare AE burden and tolerability among treatments using existing clinical trial information.

DECOMPOSING MULTIDIMENSIONAL POVERTY: A CASE STUDY OF MAMIT DISTRICT, MIZORAM

Purpose: The study intends to examine the incidence and intensity of multidimensional poverty in Mamit District of Mizoram. Methodology: This study was based on primary data which was collected through a multi-stage stratified random sampling technique. At the first stage, Mamit District was selected solely based on socio-economic indicators for conducting the survey. The second stage involved random selection of 15 villages, five villages from each block. Requisite data were then collected randomly through structured questionnaires which was designed based on the requirement for computation of Multidimensional Poverty Index. From the collected data, the incidence of poverty (headcount ratio), the intensity of poverty and MPI were computed using Alkire-Foster Methodology. The study follows the Global MPI Brief Methodological Note, 2017 in the choice of dimensions, indicators, thresholds and weights assigned to each indicator. Results: From the result of the analysis, the multidimensional poverty in the study area is moderate. The incidence of poverty across the study area is ranging between 0.32 - 0.38 while the intensity of poverty shows slight differences with negligible margin ranging between 0.39-0.411. Decompositions by indicators show that the nutrition has surpassed all other indicators with 22 percent contribution to overall MPI of Mamit district followed by year of schooling with 15 percent. Applications of this study: The findings of the study can be based for formulation of government poverty reduction policies and can be used effectively in improving the existing poverty reduction strategies in the state. KEY WORDS: Multidimensional Poverty, Decomposition, Mamit District, Mizoram.