Peranakan Ongole Research Articles

A Meta-Analysis to Determine Protein and Energy Requirements for Maintenance and Growth of Cattle in Indonesia

The meta-analysis in this study was conducted to determine the protein and energy requirements of various cattle breeds in Indonesia. A database was compiled from various research articles that involved cattle as the experimental animals, focusing on studies that reported energy and protein intake, initial body weight, and average daily gain (ADG). In total, 57 articles covering different cattle breeds in Indonesia, with 191 data points, were integrated into the database. The database specified different breeds (Aceh, Bali, Madura, Peranakan Ongole (PO), Sumba Ongole (SO), and Local) and sexes. Regressing ADG used to determine maintenance and gain requirements of dry matter, energy, and protein with dry matter intake (DMI), total digestible nutrient intake (TDNI), and crude protein intake (CPI), respectively. An intercept (where ADG= 0 kg/kg MBW/d) and a slope (required nutrient intake per unit ADG) were taken as maintenance and gain requirements, respectively. Results revealed that protein and energy requirement for maintenance (CPm and TDNm) of all cattle breeds varied in values, ranging from 0.0014 to 0.0102 kg/kg MBW/d (Madura and PO breeds) and 0.0232 to 0.0687 kg/kg MBW/d (Aceh and PO breeds), respectively. Each cattle breed in Indonesia has its particular CP and TDN requirements. Both energy and protein requirements for maintenance (CPm and TDNm) and gain (CPg and TDNg) varied in value for each breed.

Study of Ovary Follicular Profile and Estrogen Concentration of Peranakan Ongole (PO) Cows at Estrous Phase in Mountains and Lowland at Kulon Progo District, Yogyakarta

Study of Ovary Follicular Profile and Estrogen Concentration of Peranakan Ongole (PO) Cows at Estrous Phase in Mountains and Lowland at Kulon Progo District, Yogyakarta

Analisis Usaha Penggemukan Sapi Potong Berdasarkan Biaya Produksi

Research goals to increase revenue or the welfare of farmers through increased livestock production. Efforts are made is through an increase in population, the application of technology and increasing competitiveness through penerapanmanajemen farm. The research was conducted in the District Kasokandel Majalengka district with the survey method. The unit of analysis is the peasant farmers who carry out analysis of samples of beef cattle fattening to strata ownership 1-4 tails 16 people, strata ownership 5-8 tails of 7 people. The results showed that the analysis of fattening beef cattle in Sub District Kasokandel Majalengka is a side business with an average ownership of two birds per person. Nation's beef cattle are kept the nation Peranakan Ongole (PO) to the level of implementation of maintenance management is quite good except going, cages, feed and medicines. Revenue analysis of fattening beef cattle in Sub District Kasokandel Majalengkayang highest with the scale of 5-8 tails and 1-4 tail respectively and Rp 2,381,750.00 Rp 3,880,228.00. The conclusion was analysis of the beef cattle fattening business in Kasokandel District, Majalengka Regency, which is a side business with an average ownership of 2 head per person and total ownership of 1-4 head amounting to IDR 2,381,750, total ownership of 5-8 head amounting to IDR 3,880,228.60.

Population and Production Dynamic of Peranakan Ongole Cattle in The Breeding Source Area

The purpose of this research was to figure out the population structure and productivity of Peranakan Ongole cattle in the breeding source area. The research was conducted from September 2022 to August 2023 in the breeding source area for Peranakan Ongole cattle, Napis village, Tambakrejo sub-district, Bojonegoro district, East Java province. Data was obtained by recording for 1 year on 1,015 heads Peranakan Ongole cattle. Research variables include population structure and cattle productivity. The results of population structure research showed that the number of male calves was 10.34%, female calves 8.13%, bulls 17.61%, and cows 63.93%. Peranakan Ongole cattle calf crop was 55.01%, calf births to the population were 37.04% with the number of male calves born at 53.46% and the number of female calves born at 46.54%. The percentage of cows to the population was 63.94%. The percentage of mortality to the population was 2.27%. Total livestock income to the population was 12.12% and total livestock output to the population was 31.13%. The natural increase in Peranakan Ongole cattle was 34.78%. The research results showed that the Calf crop value is quite good. To increase the percentage of calf crops, it is necessary to limit and select the production of prospective and productive heifers and cows. Prospective heifers and cows who are productive and have good potential must be preserved to increase the number of births and produce good-quality calves.

Blood metabolites profile and Growth Hormone mRNA expression of Peranakan Ongole cattle fed with finishing ration containing Vinasse-molasses

<p class="MDPI17abstract"><strong>Objective: </strong>This study aimed to investigate effects of Molasses replacement with Vinasse-molasses in ration on the blood metabolite profiles and mRNA expression of <em>Growth Hormone 1</em> (<em>GH1)</em> of finishing Peranakan Ongole (PO) Cattle.</p><p class="MDPI17abstract"><strong>Methods: </strong>Ten male PO cattle in averaged 209 ± 21 kg of body weight were equally allocated to receive either molasses or Vinasse-molasses dietary treatments. The concentrate diet was contained of either 15% of molasses (control) or Vinasse-molasses. This study observed the nutrient intake, percentage of weight gain, blood metabolite profiles and <em>GH1</em> mRNA expression of PO cattle.<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The result showed that replacing molasses with vinasse-molasses reduced (P<0.05) dry matter intake (DMI) and intakes of crude protein (CP), ether extract (EE), crude fiber (CF), and total digestible nutrient (TDN). Nevertheless, Vinasse-molasses inclusion did not change (P>0.05) percentage of weight gain. Vinasse-molasses increased (P<0.05) the blood urea nitrogen level, while it did not affect (P>0.05) the <em>GH1 </em>mRNA levels.</p><p class="MDPI17abstract"><strong>Conclusions:</strong> It can be concluded that replacing molasses with vinasse-molasses does not change blood metabolite profile, <em>GH1</em> mRNA expression and performance of PO cattle. Vinasse-molasses could be applied as energy sources ingredient to replace molasses in finishing cattle feed.<strong></strong></p>

Comparation of Physiology, Diameter of Ovary and Follicle at PO Cows Treated Different Method of Estrus Synchronization

One of the biotechnology reproductive used is estrus synchronization. This study determined the body’s physiological, estradiol level, and ovarian and follicle diameter in Peranakan Ongole (PO) cow estrus synchronization using modified progesterone-PGF2α and double injection of PGF2α. This study used 10 PO cows which were divided into two groups. Group 1, cows were given a modified intravaginal sponge containing 250 mg progesterone/sponge for 6 days, followed by intramuscularly injection of PGF2ɑ 25 mg/cow on day sixth. Appearance of estrus was observed on day 7th -9th. Group 2, cows were given a double injection 25 mg/cow of PGF2ɑ intramuscularly at 14-day intervals, then estrus was observed on day 15th -17th. The vaginal temperature was checked using a thermometer, the respiratory frequency was checked by inspection of thorax movements, and the pulse rate was checked by palpation of the coccygeal artery. Ovarian and Graafian follicle diameters were examined using ultrasonography. The data were analyzed using either T-Test or descriptive test. The results showed that in cows that were synchronized to estrus using combination of modified progesterone-PGF2ɑ and double injection of PGF2ɑ, the vaginal temperature, pulse rate, and respiration frequency were 36.9±0.98 and 37.34±0.83, 57.60±12.52 and 74.80±26.59, 30.40±10.43 and 20.00±2.83 respectively. There was no significant difference in ovarian and follicle diameter (P>0.05). The conclusion was the results of body physiology were in normal conditions but still below the average of cows undergoing estrus, and there were no significant differences in ovarian and Graafian follicle diameters.

Genetic Diversity of Crossbred Cattle using Cytochrome Oxidase Subunit I (COI) Gene in South Sulawesi, Indonesia

Background: The genetic diversity information of local cattle and crossbred in South Sulawesi, Indonesia is essential for the establishment of conservation and breeding strategies. This research targeted to evaluate the phylogenetic relationship and genetic diversity of local and crossbred (CB) cattle. Methods: The present research organized at the Animal Laboratory, Professor Nidom Foundation, Surabaya, Indonesia from February to October 2021. The genome was isolated from 20 whole blood samples from seven purebreed belongs to Singosari National Artificial Insemination Center Malang, Indonesia, four crossbred in Gowa and nine crossbred in Sidrap, South Sulawesi, Indonesia. Partial sequences of mtDNA cytochrome oxidase subunit I (COI) gene were amplified employing the polymerase chain reaction technique. Result: This study revealed that a lot of single nucleotide polymorphism (SNP) found in local cattle (Bali and Peranakan Ongole) and CB cattle in Gowa and Sidrap indicated various genetic diversity can be used as a marker candidate and potential to be developed into future research knowing the correlation of the SNP with the growth rate of CB. Thus, this information will be helpful for further strategy in breeding development program.

Utilization of farmer priority on local beef cattle development strategy in Central Java, Indonesia

Abstract. Agustine R, Muzayyanah MAU, Putra ARS, Baliarti E. 2023. Utilization of farmer priority on local beef cattle development strategy in Central Java, Indonesia. Biodiversitas 24: 508-515. Indonesian local beef cattle, Peranakan Ongole (PO), is essential cattle breed predominantly raised by smallholder farmers in Central Java Province. Although it is generally perceived that raising PO cattle is not a regular source of income for the majority of farmers, however, there are few specific studies investigating the aspects and motives of farmers to keep their cattle. Therefore, this study is designed to explore the perspective of farmers leaders in Central Java, Indonesia, toward the priority aspects of raising PO cattle for their livelihoods. We selected Blora, Klaten, Grobogan, and Rembang as representatives of the study involving 29 farmers leaders with at least ten years of experience. Analytic Hierarchy Process was employed as a decision-making instrument to understand better various aspects used by PO cattle farmers. The results show that farmers consider investment function as their primary goal in raising PO cattle in all study areas. Then, farmers consider reproduction performance as the top priority aspect. In conclusion, the study revealed that smallholder farmers consider reproductive ability as a priority due to farmers make the investment function the main goal in raising PO cattle. Therefore, we recommended that farmers improve their knowledge regarding cows' reproduction through extension workers' assistance. Certainty on the PO cattle's selling price is needed so that raising PO cattle is feasible as an investment and stimulates the interest of farmers raising PO cattle.

Physiological responses of several beef cattle breeds based on environmental conditions in Beef Cattle Research Station

Physiological conditions were one factors that can affect the growth of cattle. The study aimed to determine the physiological responses of several beef cattle breeds that are reared in Beef Cattle Research Station (BCRS), Grati District, Pasuruan Regency, East Java Indonesia, was carried out by measure temperature, humidity, Temperature Humidity Index (THI), rectal temperature, respiration frequency, body temperature, Head Tolerance Coefficient (HTC) and heart rate of 159 cows consisting of Bali, Madura, Jabres, Galekan, Rambon, Peranakan Ongole (PO), Belgian Blue x PO cross, PO x Bali cross (POBA) which were observed during 4 months in pens. Data were analysed descriptively. The result of the study were ambient temperature and relative humidity of the pen ranged from 25-34.12°C and 54.88-84.27%, THI ranged from 74.92-84.97, body temperature ranged from 35.06-38.53°C, respiration frequency ranged from 20-54 times/minute, HTC ranged from 1.81-3.34, and heart rate ranged from 65-92 times/minute. It was concluded that several breeds of beef cattle in BCRS have mild to severe heat stress, high heat tolerance, respiration rate and heart rate. This condition does not cause production problem in several beef cattle, but in BB x PO cross it has potential to low productivity.

Evaluasi pendugaan bobot badan ternak sapi potong di Berkah Setia Farm Purworejo-Jawa Tengah

Beef cattle was a type of livestock that was raised to produce meat as its main product. The growing beef cattle nation in Indonesia was the limousine, simental, and peranakan ongole (PO) cattle. Berkah Setia Farm was a company engaged in beef cattle maintenance and focused on buying and selling. The purpose of this study was to find out the formula that can be used in estimating the body weight of beef cattle and to find out which formula results were closest to the actual body weight in beef cattle, especially PO cattle. The results showed that there was no 100% accurate formula, however, the formula for estimating the body weight of male PO cattle which is close to the actual body weight was calculated using the Arjodarmoko formula, with a percentage deviation of 2.6% while the largest deviation from the actual body weight was the Danish formula with a percentage deviation of 13.1%. The advice that can be given is that further research needs to be carried out with a larger number of livestock and other varieties of cattle to obtain more accurate results and close to the actual body weight.

Utilization of Vinasse-Molasses in The Finishing Ration on Growth Performance of Peranakan Ongole Cattle

Abstract Vinasse-molasses, the residue of condensed molasses is a co-product of sugar production. It is not reused in manufacturing process and become polluted. However, Its contents of 48-60% of sugar and high in potassium and sulphates. This study was to evaluate Vinasse-molasses utilization as a replacer of molasses in finishing ration on the growth performance of Peranakan Ongole Cattle. A total of 10 Peranakan Ongole cattle (initial body weight of 209 ± 21 kg) were assigned to molasses group or Vinasse-molasses group. The experiment period was lasted 68 days, preceded by 14 days adaptation period. The experimental diet was composed of 20% forage and 80% concentrate containing 15% molasses or Vinasse-molasses. Parameters observed including final body weight (FBW), average daily gain (ADG), total dry matter intake (DMI), forage intake, concentrate intake, feed efficiency (FE), and income over feed cost (IOFC). The Vinasse-molasses reduced (P<0.05) DMI and concentrate intake followed by IOFC increased (P<0.01) of cattle. However, Vinasse-molasses did not affect (P>0.05) FBW, ADG, forage intake, and FE. It can be concluded that Vinasse-molasses can be utilize as feed ingredient to replace molasses in finishing ration to support the sustainability of beef production.

Profitability of the cow-calf operation business of local peranakan ongole (PO) cattle based on the cow performance and calf price

<p><strong>Objective:</strong> <span lang="EN-US">This study aims to determine the cow-calf operation business performance of the local PO cow that can give better profitability for farmers.</span><strong></strong></p><p><strong>Methods: </strong><span lang="EN-US">The multistage purposive sampling method was used to determine of the samples. The technique of data collection was carried out using a survey method through direct interviews using a structured questionnaire to 90 beef breeders as respondent with 90 heads of productive PO local cows in Special Region of Yogyakarta. The method of analysis to determine the production performance of cows using descriptive statistics in the form of average and standard deviation. Furthermore, profitability is calculated in the form of net cash farm income with enterprise budgeting analysis.</span><strong></strong></p><p><strong>Results:</strong> <span lang="EN-US">In the current condition, the cow calf operation (CCO) business of PO cattle generated positive net cash farm income. The results of the sensitivity analysis showed that CI and calf weight were sensitive to net cash farm income. In order to get an increase in net cash farm income that was better than the current condition, it is necessary to direct the adoption of technology to shorten the calving interval to 13 months and increase the selling body weight of calves above 150 kg at the age of 6 months.</span></p><p><strong>Conclusions: </strong><span lang="EN-US">The CCO business of the local PO in the current condition has positive generate income. An increase in income that is better than the current condition can be obtained through technology adoption to shorten the CI and increase the calf sale weight.</span></p>

Characteristics of body weight and measurement of Peranakan Ongole and Brahman cattle in the tropics

Peranakan Ongole (PO) and Brahman are beef cattle breeds that are well-adapted to the tropics. The objective of this study was to evaluate the growth traits of PO and Brahman, therefore, a total of 70 cows raised in Balai Pembibitan Ternak Unggul-Hijauan Pakan Ternak (BPTU-HPT) Sembawa were selected. Subsequently, body weight and length, withers and waist height, as well as chest girth were measured at birth, 105 and 205 days, 1, 1.5, and 2 years of age. Statistical analysis using an independent sample t-test was carried out to distinguish growth traits between PO and Brahman. The results revealed that they were significantly different (P<0.05). PO had a larger body size than Brahman, particularly in terms of the withers height at 105 days, 1 and 2 years, chest girth at a year, and waist height at 105 and 205 days, 1, 1.5, and 2 years. Conversely, Brahman showed better traits than PO in terms of body weight and withers height at birth, weaning and body weight at 2 years, and average daily gain (ADG) at 205 days, 1.5, and 2 years.

Genetic polymorphisms of the 5' untranslated regions of the HSP70 gene in Indonesian cattle populations.

Background and Aim:Heat shock proteins (HSPs) are a group of proteins that play a significant role in protecting cells against cellular stress. HSP70 is a conserved, sensitive, and abundant gene associated with heat stress’s physiological adaptability. The objective of this study was to reveal the polymorphisms of the partial sequences of the HSP70 gene (5’ untranslated region [UTR]) in seven cattle populations in Indonesia.Materials and Methods:Polymerase chain reaction products (551 bp) of the HSP70 gene amplified from 102 animals representing seven cattle populations (Bali, Belgian Blue x Peranakan Ongole [PO] cross, Galekan, Jabres, Madura, PO, and Rambon) were sequenced by DNA sequencing method.Results:Fourteen single-nucleotide polymorphisms (SNPs), generally found at a low frequency, were detected. Among these SNPs, only 1117G>A, 1125A>C, and 1204T>C were polymorphic in all the analyzed breeds. A Chi-square test showed that the majority of the loci were in Hardy–Weinberg equilibrium (p>0.05). Varying levels of observed (0.050-0.571) and expected heterozygosity (0.049-0.500) were noted. The polymorphism information content values (0.048-0.375) indicated that the SNPs in the HSP70 gene showed low-to-moderate polymorphism in the studied populations. Thirty-six haplotypes were defined according to the identified SNPs, of which haplotype Hap5 (CGACGAGAGTGTCC) and Hap4 (CGACGAGAGTGCCC) were generally dominant in the studied samples. The phylogenetic tree showed a close relationship between Bali and Rambon cattle and between Galekan and Jabres cattle, while the Belgian Blue x PO crossbred cattle were farther apart.Conclusion:The polymorphisms in the 5’ UTR of the HSP70 geneidentified in this study should be further investigated in a larger population to unravel the association between the SNPs and thermotolerance in Indonesian local cattle populations.

Identification of SNP g.10428C&gt;T of Stearoyl-CoA Desaturase gene related to meat quality in Bali cattle using PCR-RFLP method

The objectives of this research was to analyze the polymorphisms of SNP g.10428C&gt;T in the SCD gene using PCR-RFLP and its association with meat charactheristics in Bali cattle. The number of 52 Bali cattle from BPTU-HTP Denpasar were used in this study and which were compared to Belgianblue (n=30), Limousine (n=14), Peranakan Ongole (PO) (n=30) and Wagyu (n=7). The polymorphism of the SCD gene was characterized by the use of the PCR-RFLP technique with HinfI enzyme restriction. The characteristics of the meat quality in Bali cattle were estimated using ultrasound imaging method. Genotyphic and allelic frequencies, heterozygosity values, and the Hardy-Weinberg equilibrium have been calculated using PopGen 1.32. The association between genotypes of SNP g.10428C&gt;T SCD gene and meat quality characteristics was calculated using t-test. The results showed that homozygous genotypes (CC and TT) and heterozygous genotypes (CT) of the SCD|HinfI gene with C (0.26) and T (0.74) allele frequencies were and in Bali cattle. The CC genotype was found in Belgian-blue, Limousine, PO, and Wagyu cattle with the allele C frequency of 1.00. The observed heterozygosity (Ho=0.44) higher than expected heterozygosity (He=0.39) showed high diversity in Bali cattle. Genotypes of SNP g.10428C&gt;T SCD gene were associated with backfat thickness, marbling score, and percentage of intramuscular fat. Thus the SNPs g.10428C&gt;T have potential as marker-assisted selection candidates for Bali cattle.

Polymorphism of follicle stimulating hormone beta sub-unit (FSH-P) gene as a molecular marker for reproductive status in Peranakan Ongole x Bali crossbred (POBA) cattle

POBA cattle is a crossbred cattle from Peranakan Ongole (PO) and Bali cattle, which is developed by the Beef Cattle Research Institute (BCRI), Ministry of Agriculture of the Republic of Indonesia. This study aimed to identify the genetic and phenotypic characteristics of POBA cattle raised in the BCRI. Genetic diversity of FSH-β gene was also determined in order to identify a possible marker for reproductive status in POBA cattle. Rambon cattle collected from Banyuwangi regency were used as comparison. A 313 bp fragment of the FSH-β gene was amplified using the polymerase chain reaction (PCR). The PCR products were sequenced and aligned to detect polymorphism. As results, a polymorphism (SNP g.2583C/T) in the FSH-β gene, which produced three genotypes (TT, CC, and CT) was detected. The frequency of each allele was 0.13 (allele C) and 0.87 (allele T). However, the FSH-β gene polymorphism did not significantly affect sperm quality, body weight, body size, and cervical condition of POBA cattle.

Analysis of farmer purposes in raising local beef cattle breed: An Analytical Hierarchy Process (AHP) approach

Farmers raise cattle for various purposes, such as a source of cash income, investment function, and socio-cultural function. The research objective was to analyze farmers’ preferences in raising Peranakan Ongole (PO) as local beef cattle breed based on the purpose of raising livestock. This study involved 8 farmers from Klaten District, Central Java. Klaten Regency was chosen as a research location because it received PO cows as a grant from the Ministry of Agriculture. Respondents were selected purposively according to the criteria of being a livestock group administrator and being experience in raising PO as local beef cattle breed for ≥10 years. Analytical Hierarchy Process (AHP) was applied to determine the priority of beef cattle criteria that are used by farmers to develop their business. There were three criteria and six factors used in this analysis. These criteria were cash income, investment function, and socio-culture function. Whereas, the six factors used were cattle breed, selling price, adaptability, reproductive ability, body size, and the physical appearance of the livestock. The results revealed that the reproductive ability has main preference among farmers due to the investment function was the main purpose in raising PO cattle.

Superovulation response of Peranakan Ongole (PO) and Simmental cows after FSH stimulation in multiple ovulation and embryo transfer program

Multiple ovulation and embryo transfer (MOET) is a reproductive technology to increase the livestock population in a short period. The success of ovary stimulation programs is influenced by an individual’s response to follicle-stimulating hormone (FSH) stimulation. In this study embryo production was carried out on local cows represented by Peranakan Ongole (PO) cows and exotic cows represented by Simmental cows. FSH stimulation was performed on 10 PO cows and 10 Simmental cows. On Day-1 Cue Mate® (progesterone) was inserted intravaginally. FSH injection (400 mg) was carried out intramuscularly from D-10 at reduced dose with 12 hours intervals (Day-10: 100 mg; Day-11: 60 mg; Day-12: 40 mg per injection). On Day-12, prostaglandin (PGF2α) was injected and Cue Mate® was removed. Artificial insemination (AI) was performed 48 hours after PGF2α injection for 3 times at 12 hours intervals. The embryo was collected 6 days after the last AI (Day-21). Superovulation response was detected on 70% of PO cows and 90% of Simmental cows. The average number of transferable embryos in Simmental cows (9.11±7.27) was higher than PO cows (7.86±7.78). This research shows that Simmental cows are more responsive to FSH stimulation, and can produce more transferable embryos than PO cows.

Ethnoveterinary study of medicinal plants used for cattle treatment in Bojonegoro District, East Java, Indonesia

Abstract. Pratama AM, Herawati O, Nabila AN, Belinda TA, Wijayanti AD. 2021. Ethnoveterinary study of medicinal plants used for cattle treatment in Bojonegoro District, East Java, Indonesia. Biodiversitas 22: 4236-4245. Bojonegoro is a rural district in Indonesia's East Java Province where farming and cattle rearing are the main economic activities. The Bojonegoro District's cattle producers employ some medicinal plants specifically for the treatment of bovine illnesses. However, no data has been reported thus far. The goal of this research was to find and document ethnoveterinary medicinal herbs for cattle cures in the Bojonegoro District. A total of 41 cattle breeders were interviewed for the study. To collect demographic and ethnoveterinary medicinal plant data, each informant was interviewed using a semi-structured questionnaire in the native language of each informant. The stastitical analysis in this study include informant consensus (Fic), Fidelity Level (FL), and Plant Part Frequency (PPF). Approximately 78.00% of the respondents are between the ages of 30 and 50, with 36.59% having only graduated from senior high school. The Peranakan Ongole (PO) is the most common breed preserved by cattle breeders. In the study area, 41 ethnoveterinary medicinal plants were mentioned by male respondents to cure cattle health problems. Digestive illnesses are the most frequent ailments in cattle treated with medicinal plants. Curcuma longa L. was the most commonly mentioned medicinal plant. A majority of the source ethnoveterinary medicinal plants were cultivated on-site and the leaves of these plants were most often used.

Identification of single nucleotide polymorphism c.957A&gt;C of PLAG1 gene and its association with growth traits in Bali cattle

The PLAG1 gene is one of the genes that affect the growth traits located on chromosome 14 in cattle. This study aims to obtain SNP of the PLAG1 gene in exon 1 and exon 2 and their association with growth traits in Bali cattle. The number of samples used was 52 samples of Bali cattle, 10 samples of Peranakan Ongole (PO), and 8 samples of Limousine cattle. Identification of SNPs PLAG1 gene was analyzed by direct sequencing method and genotyping of selected SNPs was carried out using PCR-RFLP. Association of genotypes of SNP c.957A&gt;C with growth using t-test. There were 7 SNPs in exon 2 of the PLAG1 gene, namely SNP c.339A&gt;G, c.489C&gt;T, c.795A&gt;G, c.957A&gt;C, c.1023C&gt;T, c.1056A&gt;G, and c.1353A&gt;G. SNP c.957A&gt;C was validated by PCR-RFLP using TaqI enzyme and obtained three genotypes, namely genotypes AA, AC, and CC with allele frequency A and C, respec-tively 0.10 and 0.90 in Bali cattle, while in PO and Limousine cattle were monomorphic. Genotype association of SNP c.957A&gt;C PLAG1 gene were not associated with birth weight (BW0), weaning weight at 205 days of age (WW205), yearling weight at 365 days of age (YW365), yearling weight at 730 days of age (YW730), and average daily gain (ADG). SNP c.957A&gt;C as a specific SNP for Bali cattle needs to be investigated in further research as a candidate marker for growth traits in Bali cattle.