Per Cycle Research Articles

Identity parameters on traditionally used Antiurolithiatic Herb - Scoparia Dulcis Linn.

Introduction: Scoparia dulcis Linn. locally known as Manithumbe Gida belongs to Scophularaceae family and used in medicine by the traditional practitioners for the treatment of urinary calculi. Materials and Methods: Matured plants are collected from Udupi district and authenticated. Macro-microscopic features, physico-chemical standards, HPTLC and secondary metabolites were recorded as per standard guidelines. Result: TS of leaf has shown the presence of mesophyll and bi-collateral vascular bundles. Outer cork tissue, a layer of cortex and conjoint collateral closed vascular bundles and central pith are inclusions of stem TS. Pitted and reticulate vessels are characteristic features of plant powder. Physico-chemical standards and presence of alkaloids, carbohydrates, tannin and coumarins were indicative of its chemical nature. HPTLC fingerprints are a record of its different chemical constituents. Thus the quality monograph prepared on this drug beneficial in future research.

A comparative transcriptomic approach to understanding the formation of cork

The transcriptome comparison of two oak species reveals possible candidates accounting for the exceptionally thick and pure cork oak phellem, such as those involved in secondary metabolism and phellogen activity. Cork oak, Quercus suber, differs from other Mediterranean oaks such as holm oak (Quercus ilex) by the thickness and organization of the external bark. While holm oak outer bark contains sequential periderms interspersed with dead secondary phloem (rhytidome), the cork oak outer bark only contains thick layers of phellem (cork rings) that accumulate until reaching a thickness that allows industrial uses. Here we compare the cork oak outer bark transcriptome with that of holm oak. Both transcriptomes present similitudes in their complexity, but whereas cork oak external bark is enriched with upregulated genes related to suberin, which is the main polymer responsible for the protective function of periderm, the upregulated categories of holm oak are enriched in abiotic stress and chromatin assembly. Concomitantly with the upregulation of suberin-related genes, there is also induction of regulatory and meristematic genes, whose predicted activities agree with the increased number of phellem layers found in the cork oak sample. Further transcript profiling among different cork oak tissues and conditions suggests that cork and wood share many regulatory mechanisms, probably reflecting similar ontogeny. Moreover, the analysis of transcripts accumulation during the cork growth season showed that most regulatory genes are upregulated early in the season when the cork cambium becomes active. Altogether our work provides the first transcriptome comparison between cork oak and holm oak outer bark, which unveils new regulatory candidate genes of phellem development.

Anticoagulant-Associated Heavy Menstrual Bleeding

Heavy menstrual bleeding (HMB) is defined as blood loss of >80 mL per cycle or bleeding that interferes with a woman's physical, social, and emotional

Transcriptional profiling of cork oak phellogenic cells isolated by laser microdissection.

The phenylpropanoid pathway impacts the cork quality development. In cork of bad quality, the flavonoid route is favored, whereas in good quality, cork lignin and suberin production prevails. Cork oaks develop a thick cork tissue as a protective shield that results of the continuous activity of a secondary meristem, the cork cambium, or phellogen. Most studies applied to developmental processes do not consider the cell types from which the samples were extracted. Here, laser microdissection (LM) coupled with transcript profiling using RNA sequencing (454 pyrosequencing) was applied to phellogen cells of trees producing low- and good quality cork. Functional annotation and functional enrichment analyses showed that stress-related genes are enriched in samples extracted from trees producing good quality cork (GQC). This process is under tight transcriptional (transcription factors, kinases) regulation and also hormonal control involving ABA, ethylene, and auxins. The phellogen cells collected from trees producing bad quality cork (BQC) show a consistent up-regulation of genes belonging to the flavonoid pathway as a response to stress. They also display a different modulation of cell wall genes resulting into a thinner cork layer, i.e., less meristematic activity. Based on the analysis of the phenylpropanoid pathway regulating genes, in GQC, the synthesis of lignin and suberin is promoted, whereas in BQC, the same pathway favors the biosynthesis of free phenolic compounds. This study provided new insights of how cell-specific gene expression can determine tissue and organ morphology and physiology and identified robust candidate genes that can be used in breeding programs aiming at improving cork quality.

Comparison of the effect of different disinfectants on endoscopic disinfection and durability

Objective To explore the effect of different disinfectants on endoscopic disinfection and durability. Methods A total of 428 endoscopes were selected from the endoscopic center of our hospital from January 2016 to September 2016, and they were randomly divided into control group and observation group, each group in 214cases.The control group was treated with glutaraldehyde.The observation group used o-phthalaldehyde treatment disinfection.The sampling site was the endoscopic biopsy cavity surface, sterile syringe was used to extract 10ml containing neutralizer buffer, into the endoscopic biopsy to be tested, and then 15mL sterile test tube from the biopsy outlet collection, was checked within 2h.The sterilization pass rate was evaluated, the disinfectant disinfection endoscopic cavity and the number of surface colonies, disinfectant endoscopic durability in the two groups were compared. Results The disinfection rate was 89.25% in the control group and 97.20% in the observation group, the difference of the qualified rate of endoscopic disinfection between the two groups was statistically significant (χ2=10.69, P=0.001). The endoscopic cavity and the number of surface colonies of the observation group were lower than those of the control group (t=3.17, P=0.00; t=26.76, P=0.00). The numbers of disinfection per cycle and per day in the observation group were higher than those in the control group, the differences were statistically significant (t=94.44, P=0.00; t=23.94, P=0.00). Conclusion The use of o-phthalaldehyde for endoscopic disinfection is better, more durable, suitable for endoscopic room to promote the use. Key words: Disinfectant; Endoscopic; Disinfection qualified rate; Durability

Optimizing Douglas-fir bark liquefaction in mixtures of glycerol and polyethylene glycol and KOH

Abstract The outer bark of Douglas-fir (Pseudotsuga menziesii) has a significant amount of cork tissue that may be an important source of chemicals derived from its natural polymers, suberin, cellulose, hemicelluloses and lignin. The present work focuses on the polyalcohol liquefaction of Douglas-fir bark with glycerol and polyethylene glycol (PEG) in order to obtain a liquid that can be further processed to other chemicals and products. The results show that Pseudotsuga bark can be liquefied in a significant percentage in presence of alkali. The best liquefaction yield was obtained with 6% KOH as agents. Although the use of a cosolvent is favorable, good liquefaction yields can also be obtained by glycerol alone. Lower temperatures are favorable as they lead to acceptable liquefaction yields. FTIR-ATR studies showed that all the structural compounds of the bark were attacked and depolymerized. The process tested has a high potential for generation of value-added products from liquefied Douglas-fir bark.

A Study of TPCLT(Twice Per Cycle Left-Turn) Operation Impact at Three-legged Signalized Intersection

A Study of TPCLT(Twice Per Cycle Left-Turn) Operation Impact at Three-legged Signalized Intersection

Reconstructing the suberin pathway in poplar by chemical and transcriptomic analysis of bark tissues.

The tree bark periderm confers the first line of protection against pathogen invasion and abiotic stresses. The phellogen (cork cambium) externally produces cork (phellem) cells that are dead at maturity; while metabolically active, these tissues synthesize cell walls, as well as cell wall modifications, namely suberin and waxes. Suberin is a heteropolymer with aliphatic and aromatic domains, composed of acylglycerols, cross-linked polyphenolics and solvent-extractable waxes. Although suberin is essentially ubiquitous in vascular plants, the biochemical functions of many enzymes and the genetic regulation of its synthesis are poorly understood. We have studied suberin and wax composition in four developmental stages of hybrid poplar (Populus tremula x Populus alba) stem periderm. The amounts of extracellular ester-linked acyl lipids per unit area increased with tissue age, a trend not observed with waxes. We used RNA-Seq deep-sequencing technology to investigate the cork transcriptome at two developmental stages. The transcript analysis yielded 455 candidates for the biosynthesis and regulation of poplar suberin, including genes with proven functions in suberin metabolism, genes highlighted as candidates in other plant species and novel candidates. Among these, a gene encoding a putative lipase/acyltransferase of the GDSL-motif family emerged as a suberin polyester synthase candidate, and specific isoforms of peroxidase and laccase genes were preferentially expressed in cork, suggesting that their corresponding proteins may be involved in cross-linking aromatics to form lignin-like polyphenolics. Many transcriptional regulators with possible roles in meristem identity, cork differentiation and acyl-lipid metabolism were also identified. Our work provides the first large-scale transcriptomic dataset on the suberin-synthesizing tissue of poplar bark, contributing to our understanding of tree bark development at the molecular level. Based on these data, we have proposed a number of hypotheses that can be used in future research leading to novel biological insights into suberin biosynthesis and its physiological function.

Clinical outcome analysis of mild-stimulation in poor ovarian responders

Objective To explore the clinical outcomes of different mild-stimulation in poor ovarian responders in vitro culture/intracytoplasmic injection (IVF/ICSI) to provide the evidence for clinical treatment, and investigate clinical parameters including pre-ovulation rate, rate of oocytes retrieved, fertilization rate, good quality embryo rate, and pregnancy rate per cycle. Methods The clinical date of a total 180 IVF cycles of infertile patients undergone from Jan 2013 to May 2016 in medical reprodution center of Xiang Tan Central Hospital. According to the different methods of controlled ovarian hyperstimulation (COH), the cycles were divided into 3 groups: group A with 60 cycles of early five days using clomiphene, group B with 60 cycles of persistent using clomiphene, and group C with 60 cycles using artificial luteal-phase ovarian stimulation protocol. Results The basic situations in three groups were no significant difference ( P>0.05). Compared to groups B and C, the pre-ovalution rate was more in group A (P<0.05), and the rate of oocytes retrieved, fertilization rate, good quality embryo rate, and preganacy rate per cycle were higher in groups B and C (P<0.05). The rate of oocytes retrieved, fertilization rate, good quality embryo rate, and preganacy rate per cycle were no significant difference among gourps B and C. The good quality embryo rate and preganacy rate per cycle in groups B and C were higher than group A, and group B was higher than group A (P<0.05). Conclusions Persistent using clomiphene or persistent using clomiphene in COH could decrease the rate of pre-ovalution, and get more rate of oocytes retrieved, good quality embryo rate, and preganacy rate per cycle. Key words: Ovulation induction; Ovarian diseases/DT

Cork of Douglas-fir bark: Impact of structural and anatomical features on usage

Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) is one of the best conifer timber species. Its bark contains a substantial proportion of cork that may have a valorization potential, given adequate structural and cellular features. This study was made here onbark samples from mature trees from the north and central mountains of Portugal. The area proportion of cork was determined by image analysis, the cork tissue was observed with electron scanning microscopy (SEM) and cell dimensions measured.The cork is not continuous within the rhytidome, and the layers are interspersed with phloem regions. The cork layers are not continuous along the tangential or axial directions. Older trees contain on average a thicker rhytidome and a higher proportion of cork. The cork tissue was characterized by the presence of extensive areas of cells that are crushed or completely collapsed, making up a compressed and very compact structure with patches of uncompressed cork. The compression occurs in the radial direction and is clearly observed in transverse and radial sections. In the uncompressed regions the majority of the cork cells are hexagonal and pentagonal prisms stacked base-to-base and aligned in the radial direction in parallel rows. On average, the prism height and base area are 55μm and 1388μm2, respectively, with a 1.3μm cell wall thickness. To obtain pure cork fractions from Douglas-fir bark, trituration and fractionation processes are needed. Also the use of Douglas-fir cork as a cellular material will be restricted by the extensive cell compression.

ГИСТОХИМИЧЕСКОЕ ИЗУЧЕНИЕ КЛЕТОК КСИЛЕМЫ У IRIS SIBIRICA L. В КУЛЬТУРЕ IN VITRO

This paper presents the data content of the lignin from annual plants-regenerants Iris sibirica is comparable to the content of a 6-year-old intact plants. With the aim of identifying conditions of accelerated lignification studied the structure of the shoots of Iris sibirica , grown on artificial nutrient media, using the methods of histochemistry. Peculiarities of the formation of the xylem from Iris sibirica on artificial nutrient media. Very quickly regenerants developed a complex system consisting of conducting bundles containing sieve tubes, vessels and tracheids, and as a network of gidrozit. Hydrocity Iris sibirica in its structure is tracheids with significiance the swelling, but, in contrast to tracheids and vessels of xylem (they are formed on the basis of procube or cambium – lateral special primary or secondary meristem), hydrocity differentiated from the cells of permanent tissues (like phellogen), which probably at the time of differentiation possessed meristematic activity. From Iris sibirica hydrocity thick layer envelops the conductive beam and accompanied him along the escape at a certain height. Due to the formation of the thick fabric of significiance tracheal elements, young regenerants Iris sibirica was high lignin content. The study of the differentiation of xylem elements in the laboratory can serve as a model for our understanding of the processes of formation of wood.

The Woody-Preferential Gene EgMYB88 Regulates the Biosynthesis of Phenylpropanoid-Derived Compounds in Wood.

Comparative phylogenetic analyses of the R2R3-MYB transcription factor family revealed that five subgroups were preferentially found in woody species and were totally absent from Brassicaceae and monocots (Soler et al., 2015). Here, we analyzed one of these subgroups (WPS-I) for which no gene had been yet characterized. Most Eucalyptus members of WPS-I are preferentially expressed in the vascular cambium, the secondary meristem responsible for tree radial growth. We focused on EgMYB88, which is the most specifically and highly expressed in vascular tissues, and showed that it behaves as a transcriptional activator in yeast. Then, we functionally characterized EgMYB88 in both transgenic Arabidopsis and poplar plants overexpressing either the native or the dominant repression form (fused to the Ethylene-responsive element binding factor-associated Amphiphilic Repression motif, EAR). The transgenic Arabidopsis lines had no phenotype whereas the poplar lines overexpressing EgMYB88 exhibited a substantial increase in the levels of the flavonoid catechin and of some salicinoid phenolic glycosides (salicortin, salireposide, and tremulacin), in agreement with the increase of the transcript levels of landmark biosynthetic genes. A change in the lignin structure (increase in the syringyl vs. guaiacyl, S/G ratio) was also observed. Poplar lines overexpressing the EgMYB88 dominant repression form did not show a strict opposite phenotype. The level of catechin was reduced, but the levels of the salicinoid phenolic glycosides and the S/G ratio remained unchanged. In addition, they showed a reduction in soluble oligolignols containing sinapyl p-hydroxybenzoate accompanied by a mild reduction of the insoluble lignin content. Altogether, these results suggest that EgMYB88, and more largely members of the WPS-I group, could control in cambium and in the first layers of differentiating xylem the biosynthesis of some phenylpropanoid-derived secondary metabolites including lignin.

Chemical and cellular features of virgin and reproduction cork from Quercus variabilis

Quercus variabilis (Chinese cork oak) has a substantial cork in the periderm that regenerates if removed, allowing a potential sustainable production. Samples of virgin and reproduction cork of Q. variabilis from China were characterized and compared with Q. suber cork. Virgin and reproduction cork are chemically very similar: on average 0.9% ash, 9.2% extractives, 37.8% suberin, and 28.5% lignin. The suberin composition of Q. variabilis cork is reported for the first time. It is composed mostly by saturated ω-hydroxyacids and substituted ω-hydroxyacids, including mainly 22-hydroxydocosanoic acid (20–23% of the total compounds) and 18-hydroxy-9-octadecenoic acid (17–22%). Comparison with Q. suber cork shows differences, stressing that suberin composition is species specific. The lipophilic extractives include mainly triterpenes (48.6–56.9%) especially friedelin and betulinic acid.Q. variabilis cork has the typical cellular characteristics of cork tissues with radially aligned cells without intercellular voids. Cell wall undulations were conspicuous, especially in virgin cork.Q. variabilis cork may be a complementary raw-material to Q. suber cork for agglomerated products. Despite similarities with the commercial cork, Q. variabilis cork has differences that impact unfavourably on properties e.g. higher density and compressive strength.

Acceptability, tolerability, and satisfaction of a contraceptive vaginal ring (the NuvaRing) among Thai women

Background: The NuvaRing has demonstrated efficacy and acceptability in a number of clinical studies mostly in western countries. Because of cultural differences and self-administration of the device, it may not be wellaccepted by Thai women. Objectives: To assess the acceptability, tolerability and satisfaction of Thai women who started to use the NuvaRing. Methods: The levels of acceptance by 39 clients who began using the NuvaRing were reviewed in this retrospective observational cohort study of clients at a family planning clinic in Bangkok. Ring-related and other adverse events were recorded at the end of second, fourth, and sixth cycles. Results: Included in this study were clients (38/39; 97%) who used the NuvaRing for six cycles consecutively. Difficulties in removing the NuvaRing were experienced by 13% of the participants and 49% felt transient vaginal discomfort; however, these problems resolved over time. Approximately one third of clients had asymptomatic watery discharges. Of the total of 229 cycles 95.1% were as usual with scheduled bleeding. Seventy-one percent of the clients were very satisfied. Before and after 6 cycles of using the NuvaRing, there were no significant clinical differences between body weight, blood pressure, duration of period, and amount of pads used per cycle found using paired 2-sample t tests. Conclusions: NuvaRing has demonstrated no significant effects on regular cycle continuation and had very few clients experienced adverse events. The acceptability of this device and satisfaction of using it were partly owing to structural, comprehensive counseling, and support provided by the family planning staff. Keywords: Acceptability, contraceptive vaginal ring, NuvaRing, satisfaction, tolerability

Overexpression of INCREASED CAMBIAL ACTIVITY, a putative methyltransferase, increases cambial activity and plant growth.

Cambial activity is a prerequisite for secondary growth in plants; however, regulatory factors controlling the activity of the secondary meristem in radial growth remain elusive. Here, we identified INCREASED CAMBIAL ACTIVITY (ICA), a gene encoding a putative pectin methyltransferase, which could function as a modulator for the meristematic activity of fascicular and interfascicular cambium in Arabidopsis. An overexpressing transgenic line, 35S::ICA, showed accelerated stem elongation and radial thickening, resulting in increased accumulation of biomass, and increased levels of cytokinins (CKs) and gibberellins (GAs). Expression of genes encoding pectin methylesterases involved in pectin modification together with pectin methyltransferases was highly induced in 35S::ICA, which might contribute to an increase of methanol emission as a byproduct in 35S::ICA. Methanol treatment induced the expression of GA- or CK-responsive genes and stimulated plant growth. Overall, we propose that ectopic expression of ICA increases cambial activity by regulating CK and GA homeostasis, and methanol emission, eventually leading to stem elongation and radial growth in the inflorescence stem.

A clinical study of salvage radiotherapy for supraclavicular lymph node metastasis in patients with esophageal cancer

Objective To evaluate the efficacy of salvage radiotherapy for supraclavicular lymph node metastasis (SLNM) after initial treatment in patients with esophageal cancer. Methods A total of 117 patients with SLNM after radical resection for esophageal cancer were enrolled as subjects from 2006 to 2012. All patients received three-dimensional radiotherapy with 1.8-2.0 Gy per cycle, 5 cycles a week. The survival rates were calculated using the Kaplan-Meier method and analyzed using the log-rank test. The Cox model was used for multivariate analysis. Results The follow-up rate was 100%. In all the patients, the 1-and 3-year overall survival (OS) rates were 38.5% and 14.1%, respectively. The 1-and 3-year OS rates were significantly higher in patients treated with salvage radiotherapy or radiochemotherapy (n=100) than in patients without any salvage treatment (n=17)(42% vs. 18%, P=0.008; 17% vs. 0%, P=0.008). The patients treated with radiochemotherapy (n=32) had significantly higher 1-and 3-year OS rates than those treated with radiotherapy alone (n=68)(59% vs. 34%, 36% vs. 11%, P=0.002) or without any salvage treatment (n=17)(59% vs. 18%, 36% vs. 0%, P=0.002). Patients without visceral metastasis (n=80) had significantly higher 1-and 3-year OS rates than those with visceral metastasis (n=37)(44% vs. 27%, P=0.002; 22% vs. 0%, P=0.002). Patients with supraclavicular doses of ≥60 Gy in salvage radiotherapy (n=75) had significantly higher 1-and 3-year OS rates than those with supraclavicular doses of<60 Gy in salvage radiotherapy (n=25)(75% vs. 25%, P=0.000; 24% vs. 8%, P=0.000). The multivariate analysis using the Cox model showed that supraclavicular doses of ≥60 Gy, mediastinal metastasis, visceral metastasis, and salvage treatment method were independent factors for survival (P=0.001, 0.015, 0.009, 0.025). Conclusions Salvage radiotherapy can improve the survival of patients with SLNM in esophageal cancer. Salvage radiotherapy or radiochemotherapy is highly recommended for patients with SLNM alone. A radiation dose of ≥60 Gy in salvage radiotherapy improves survival in patients. Key words: Esophagus neoplasms; Supracevicular lymph node metastasis; Salvage treatment; Prognosis

Transferring Fewer Embryos Makes Sense

In the U.S., in vitro fertilization (IVF) is beset with high rates of multiple pregnancies (mostly twins) due to the common practice of transferring ≥2 embryos per cycle. Investigators analyzed CDC data for IVF cycles initiated during 2013 to test the hypothesis that clinics with higher rates of elective single embryo transfer (eSET) would have lower incidence of multiple births while maintaining high pregnancy rates. The clinic-level analysis was focused on …

Efficacy of hypofractionated stereotactic radiotherapy combined with temozolomide for large brain metastases: a prospective clinical study

Objective To analyze the efficacy and safety of hypofractionated stereotactic radiotherapy (FSRT) combined with temozolomide (TMZ) for large brain metastases (BMs) in a prospective phase Ⅱ clinical trial. Methods From 2010 to 2015, a total of 33 patients were enrolled as subjects. The median Karnofsky Performance Status scores before and after treatment were 70 and 80, respectively. The major primary tumor was non-small cell lung cancer (57.6%). The brain metastasis had a diameter of ≥3 cm or a volume of ≥6 cm3. The radiation dose was 52 Gy in 13 fractions or 52.2 Gy in 15 fractions. Patients received TMZ at a dose of 75 mg/m2 per day concurrently. The radiotherapy was followed by 6 cycles of adjuvant treatment with TMZ (150 mg/m2, days 1-5, 28 days per cycle). Patients were reexamined by magnetic resonance imaging (MRI) during the treatment. The radiation field would be shrunk if the gross target volume (GTV) was reduced by ≥20%. The treatment outcomes were evaluated by MRI at 2-3 months after treatment. Results The total numbers of tumors and GTVs were 95 and 38, respectively. Twenty-four (63%) out of the 38 GTVs had a volume larger than 10 cm3 and the median GTV was 15.3 cm3(5.7-142.8 cm3). Twenty-two (67%) out of the 33 patients achieved field shrinking during the treatment, and the median reduction rate of GTV was 44%(21%-88%). The median total dose was 59.5 Gy, and 100% and 21.2% of patients completed the concurrent and adjuvant treatment with TMZ, respectively. In all patients, the overall response rate was 97.0%; the 1-year local control, intracranial progression-free survival, and overall survival rates were 97%, 70%, and 62%, respectively; the median survival time was 15.3 months. The main adverse reactions were grade 1-2 nausea and vomiting. One patient got grade 3 liver function impairment. Conclusions FSRT combined with TMZ is a safe and effective approach for treating large BMs. More than 50% of patients can achieve field shrinking to shorten treatment duration and reduce toxicity. Clinical Trial Registry ClinicalTrials.gov, registration number: NCT02654106. Key words: Neoplasms, brain metastases/radiotherapy; Radiotherapy, hypofractionated; Neoplasms, brain metastases/chemotherapy; Chemotherapy, temozolomide; Large volume

The characteristics of cork and hypodermis tissues and cracking in Asian pear (Pyrus pyrifolia cv. Mansoo)

Mansoo is a russet pear cultivar cultivated in Korea and susceptible to cracking at maturity. Cracked regions were studied using light (LM), stereo (SM), and scanning electron microscopy (SEM) to compare the prevalence of this phenomenon in Mansoo and Niitaka cultivars. The initial cracking stage was observed as a short-length-shaped crack (SLSC) on the lenticel, followed by an enlargement and extension of the cracked region as a long-length-shaped crack (LLSC). Although the cracked region on the lenticel was discovered with the naked eye, cracking also occurred in the surrounding filling tissue region, which has a thin hypodermis because of a crevice of cork tissue. The cracking occurred in the intercellular space of parenchymal tissue and between stone cell clusters. Mansoo fruit has thinner, more irregular, and less numerous hypodermis layers than that of Niitaka. Stone cell clusters in the peripheral parenchyma were concentrated because they were larger and had a smaller distance between them in Mansoo cultivars than in Niitaka. These results demonstrate that the morphological and anatomical features observed in Mansoo fruit are cracking susceptibility factors.

Assisted reproductive technology: Experience from a public tertiary institution in north central Nigeria

Background: According to the World Health Organization, more than 180 million couples globally suffer from infertility, the majority being residents of developing countries. Assisted reproductive technologies (ARTs) offer a chance at parenthood to couples, who until recently would have had no hope of having a “biologically related” child. Objectives: This study aimed to determine pregnancy outcomes following assisted conception. Materials and Methods: This is a prospective study of 104 clients who underwent the procedure of ART between January 1, 2012 and December 31, 2016 at the ART unit of University of Ilorin Teaching Hospital, Ilorin, Nigeria. Results: Of the 510 clients who had infertility consultation at the ART clinic, 104 (20.4%) underwent ART procedures. The patients aged 27–46 years with a mean age of 33 ± 4.0 years. More than half (58.7%) had primary infertility. Their duration of infertility ranged from 1 to 20 years (4.6 ± 2.9 years). Majority (81.7%) had conventional in vitro fertilization while 19 (18.3%) had intracytoplasmic sperm injection. Thirteen (12.5%) cases of cycle cancellation and 11 (11.7%) cases of mild-to-moderate ovarian hyperstimulation syndrome were recorded. The clinical pregnancy rate per cycle started was 39.4%. However, 9/41 (22%) resulted in spontaneous miscarriages and 32 (6 sets of twin, 25 singleton, and 1 high-order multiple births) were successfully delivered, giving a live birth rate per cycle started of 30.8%. Pregnancy outcomes were not significantly affected by age of the women, types of infertility, and duration of infertility (P > 0.05). Conclusion: The outcomes of ART procedures in a resource-limited country like ours are encouraging. This underscores the need to encourage ART in public tertiary institutions in Nigeria through the support of government and nongovernmental organizations for the benefit of infertile couples who were hitherto hopeless.