Peptide-based Vectors Research Articles

Peptide-based Vectors for blood–brain Barrier Targeting and Delivery of Drugs to the Central Nervous System

Peptide-based Vectors for blood–brain Barrier Targeting and Delivery of Drugs to the Central Nervous System

Gramicidin A-based peptide vector for intracellular protein delivery

The development of the peptide-based vectors for the intracellular delivery of biologically active macromolecules has opened new prospects of their application in research and therapy. Earlier the amphipathic cell-penetrating peptide (CPP) Pep-1 was reported to mediate cellular uptake of proteins without covalent binding to them. In this work we studied the ability of a series of membrane-active amphipathic peptides, based on the gramicidin A sequence, to transport a model protein across the eukaryotic cell membrane. Among them the positively charged Cys-containing peptide P10C demonstrated the most effective β-galactosidase intracellular delivery. Besides, this peptide was shown to form noncovalent associates with β-galactosidase as judged from electrophoresis and enzymatic activity assays. In addition, a series of new gramicidin analogues were prepared and the effect of N-terminus modification of gramicidin on the protein transduction efficiency was studied.

Peptide-based vectors for neuron-specific gene delivery

Peptide-based vectors for neuron-specific gene delivery

Peptide Mini-Vectors for Gene Delivery.

The effective treatment of diseases by gene therapy may be a step nearer with the synthesis of two new peptide-based vectors for the delivery of genes to cells. A combination of two peptides-one containing an Arg-Gly-Asp integrin-binding motif, the other a fusogenic moiety-delivers genes almost as effectively as cationic liposomes.

Peptide Mini-Vectors for Gene Delivery

The effective treatment of diseases by gene therapy may be a step nearer with the synthesis of two new peptide-based vectors for the delivery of genes to cells. A combination of two peptides—one containing an Arg-Gly-Asp integrin-binding motif, the other a fusogenic moiety—delivers genes almost as effectively as cationic liposomes.