Penicillin Group Research Articles

Symmetrical drug-related intertriginous and flexural exanthem with itraconazole: The unusual reaction with a frequently used drug

Symmetrical drug-related intertriginous and flexural exanthem (SDRIFE) is a rare, benign and self-limiting cutaneous adverse drug reaction. It presents as an erythematous rash distributed symmetrically on the gluteal and intertriginous areas with no systemic affection. SDRIFE is most commonly reported after exposure to antibiotics, particularly those in the penicillin group. Here, we present a case of SDRIFE associated with itraconazole. A 15-year-old boy presented with a dermatophytic infection for which itraconazole was prescribed. After 10 days of drug intake, he presented with diffuse erythema and scaling over the buttocks. The rash continued to progress to other intertriginous areas and was associated with itching and a burning sensation. On examination, an erythematous scaly macular rash symmetrically involving groins, thighs, neck, axilla, flexural aspect of arms, popliteal fossa and upper part of chest was noted. There was complete resolution of the lesions after 2 weeks of stopping itraconazole. The patient was, thus, diagnosed with SDRIFE due to itraconazole, based on the drug history and clinical profile. Although SDRIFE caused by itraconazole is exceedingly rare, increasing awareness among dermatologists can facilitate early identification and prompt discontinuation of the medication.

Antibiotics Self-Medication Among Medical and Non-Medical Students of Omar Al-Mukhtar University

Self- consumption of antibiotics is being a big concern over the world, excessive as well as incorrect using of antibiotics give rise to various complications like spreading resistance of antibiotics, delaying in the disease diagnosis, masking the symptoms and making an infection worse which make it hard to find the appropriate treatment. This survey was conducted to determine prevalence, pattern of consumption, possible reasons, awareness as well as knowledge about self-consumption of antibiotics among both medical and non-medical students at Omar Al-Mukhtar University, Al-Bayda, Libya. A cross- sectional survey was carried out targeting both medical and non-medical students at Omar Al-Mukhtar University, Al-Bayda, Libya. 508 students were included in this research, 282 were medical students and 226 were non-medical students. The information was gathered from a printed questionnaire and an electronic questionnaire, which has been analyzed by using the Microsoft Excel software. Our research noticed that, the mean age was 22.215% for all participants, including males 259 (51.19%) and females 247 (48.81%). Approximately, 63.58% of Students practiced self-medication with antibiotics.59.54% of respondents picked the pharmacist as a preferred source of antibiotics. Moreover 20.6% of the students experienced antibiotics to treat sore throat during 6 months ago, while 19.08% of them had flu. Most students (72.18%) had a knowledge about the adverse effects of antibiotics while, 27.81% of them did not have any idea about the adverse effects. Fortunately, most students (70.7%) did not suffer any side effects. Penicillin groups (penicillin 20.4% and amoxicillin 17.32%) were the common self-consumed antibiotics between Omar Al-Mukhtar University students. Most students (70.79%) did not complete their antibiotics course and 4.42% of them had antibiotics for >7days. Self-consumption of antibiotics is common and widely spread between medical and non-medical Students at Omar Al-Mukhtar University, Al-Bayda, Libya. There is a pressing need to enforce legislation to regulate antibiotics access, more awareness campaigns are needed to spread knowledge about the possible complications of antibiotics between the students.

Antibiotic Prescription Practice and Resistance Patterns of Bacterial Isolates from a Neonatal Intensive Care Unit: A Retrospective Study from Jordan.

Background/Objectives: Neonatal sepsis is a systemic inflammation in neonates caused by bacteria, viruses, or fungi that can progress into severe conditions. In developing countries, neonatal sepsis is a major cause of mortality and a major public health issue with a high prevalence. This study aims to evaluate the antibiotic prescription practice and resistance patterns of bacterial isolates from the neonatal intensive care unit (NICU) at the largest governmental hospital in Amman, Jordan. Methods: This was a retrospective cross-sectional study. The antibiotic prescription practice and resistance patterns of bacterial isolates from the NICU at Al Basheer Government Hospital in Amman, Jordan, were evaluated. The hospital's microbiology lab database and medical records were the sources of the retrospective data collection. Results: A total of 266 neonates treated with antibiotics were assessed. The findings showed that most neonates had late-onset sepsis (LOS) (65.4%). The penicillin group of antibiotics (ampicillin) was the most highly prescribed first empiric antibiotic for LOS and early-onset sepsis (EOS) (61.7%). Aminoglycosides (60.9%) were the most prescribed antibiotics as a second empiric treatment for EOS and LOS. The culture results showed that resistance to antibiotics was as follows: 15.4% of the culture samples were resistant to penicillin (Micrococcus and Viridans streptococci), 13.9% were resistant to cefotaxime (Klebsiella pneumoniae and Viridans streptococci), 13.2% were resistant to cefoxitin (Klebsiella pneumoniae and Staphylococcus epidermidis), and 12.4% were resistant to oxacillin (Klebsiella pneumoniae and Staphylococcus epidermidis). Conclusions: This retrospective study sheds light on the antibiotic prescription practice and resistance patterns of bacterial isolates from newborns with sepsis. The results highlight the high rates of antibiotic resistance. These findings underline the urgent need for improved antibiotic stewardship and infection control strategies to prevent resistance from spreading further.

Antibiotic Resistance in Cases of Bacterial Meningitis: Literature Review

Introduction: Meningitis is an infectious disease that attacks the cerebral membrane or swallows. Meningite is among the ten most dangerous diseases in the world. The disease can affect both children and adults. The cause of this disease can be bacteria, viruses, fungi, or aseptic. Most cases of viral meningitis usually heal themselves and are not fatal, but in severe cases, such as bacterial, biased, life-threatening. Methods: Articles related to bacterial meningitis taken from databases like Google Scholar, Pubmed and Portal Garuda over the last 5 years (2020- 2024). Use keywords: "Bacterial meningitis", "symptoms", "therapy", "antibiotic resistance Results: Based on studies that have been carried out, antibiotic resistance rates in cases of bacterial meningitis are very high and vary. Streptococcus pneumonia as one of the most common causes has high levels of resistance to antibiotics of the penicillin group. Conclusion: With the growing number of rapid, reliable and accurate diagnostics and follow-up examinations, it can cope with the incidence of antibiotic resistance in bacterial meningitis.

Investigation of virulence factor genes and biofilm formation of antibiotic resistant clinical E.coli isolates.

The aim of this study is to investigate the antibiotic sensitivity, presence of virulence genes and biofilm formation capacity of 90 clinical E. coli isolates. The presence of virulence genes in E.coli isolates were investigated by PCR. Ninety clinical isolates of E.coli were subjected to biofilm quantitative analysis using the semi-quantitative crystal violet staining method. it was observed that the isolates were resistant to quinolone, cephalosporin, aminoglycoside, carbapenem and penicillin group antibiotics. The presence of virulence factor genes were observed in a total of 86/90E. coli. The highest rate of fim (92.2%) virulence factor gene was detected in the strains. Afa, pap, cnf, sfa, hly were detected in 30%, 13%, 13%, 3.3%, 2.2% respectively. Also, 13 different virulence factor gene patterns were determined in 90E. coli isolates. Of the 90E. coli isolates whose biofilm-forming capacities were evaluated, 42 were found to have biofilm-forming capacity. Of these 26 (28.8%) the weak, 12 (13.3%) moderate and 4 (4.4%) strong biofilm-forming. Also, statistical analysis was performed to investigate the relationship between virulence factor genes and biofilm formation, and none of the 7 genes analyzed showed a significant relationship with biofilm formation. since pathogenic E. coli is an important public health problem, investigating antibiotic resistance, virulence factor genes and biofilm formation in bacterial pathogens is important for better treatment options.

Detection of Uropathogenic Escherichia coli Virulence Factors’ fimH and fliC H7 in Urinary Tract Infections and Their Association with Antibiotics Resistance among Sudanese Children

Background UTIs are one of the most common pathological conditions in both community and hospital settings. A broad spectrum of pathogens causes UTIs, it has been estimated that about 80% to 90% of cases worldwide develop UTI each year. However, the most frequent agent of community-acquired UTI is Uropathogenic Escherichia coli (UPEC), Some of UPEC genes have been associated with disease and antibiotic resistance. The presence of multidrug-resistant UPEC harboring several virulence factors, is a major risk factor for inpatients. This study aimed to detect the presence of virulence factors; fimH and fliC H7 genes and their possible association with antibiotic resistance, to evaluate the relation between virulence factors and antibiotic resistance among UPEC isolated from Sudanese symptomatic children’s patients with UTI, in Khartoum State- Sudan. Methods Sixty-six urine samples from study participants with symptomatic UTIs have been identified by clinician from two hospitals as UPEC using the conventional microbiological tests. All the isolates were subjected to antimicrobial sensitivity test using disk diffusion and polymerase chain reaction (PCR) assay to identify the presence of two putative virulence factors; fimH and fliC H7, and their association with antimicrobial resistance. Results Our results have shown that Amoxcacillin (penicillin group) was consistently the most resistance active (90.9%) of the systemically active antimicrobials, followed by Sulfamethoxazole (89.4%). On the other hand, sensitivity to nitrofurantoin (nitrofuran) was found to be the highest (89.4%). FimH gene has the highest prevalence among E. coli isolates (63.6%), followed by fliC gene (53.0%). A statistically significant association was found between the presence of fimH gene and resistance of E. coli to Polynaphethol with Chi-square p-value = 0.016 and odds ratio =5.353. Another significant association was found with resistance of E. coli to Chloramphenicol (Chi-Square p-value = 0.038, odds ratio = 3.103) and presence of fimH gene, also there was a significant association between the presence of fliC H7 gene and resistance of E. coli to Cephalexin with a (Chi-Square p-value = 0.001 and odds ratio = 5.869) Conclusions The study highlighted the prevalence of UPEC isolates which harbored the virulence factors and their association with antibiotic resistance which possibly cause the urine disease. The outcome of the study may represent targets for improving the regimen used and therapeutics against UPEC in Sudan.

Self-Reported Antibiotics Usage, Allergies and Resistance of Albanian Patients from a Dental Perspective: A Preliminary Questionnaire-Based Survey.

The paper aims to assess the level of antibiotic use and the antibiotic types used generally in dentistry and identify self-reported allergies and resistance related to them. The data were collected between March and May 2024 via an electronic questionnaire on self-reported antibiotic usage, antibiotic allergies and resistances among individual in Albania. A total of 477 individuals (83% females, 17% males) with a mean 33 ± 13 (min 17; max 73) years of age completed the questionnaire. Overall, 88% of the population reported having used antibiotics before the questionnaire, among whom 56% used them for dental reasons. An average of 5% reported being allergic, mainly to the penicillin group, while 5% claimed to have undergone an antibiogram analysis before taking the prescribed antibiotics, with most of them showing resistance to the penicillin group. Based on the findings of this study, the high prevalence of antibiotics usage in dentistry encourages further deepening studies and training adapting global guidelines for antibiotics indications in dentistry to the level of antibiotics allergies and resistances of this specific country.

The Interactions of Resveratrol and Sodium Valproate on Penicillin-Induced Epilepsy Model: Electrophysiological and Molecular Study.

Epilepsy represents the most prevalent chronic neurological disease, characterized by spontaneous recurrent seizures. In experimental epilepsy models created by different methods, resveratrol has been demonstrated to reduce epileptiform activity and exhibit neuroprotective properties. A penicillin-induced model of epileptogenesis was used to investigate the effects of resveratrol and its combination with sodium valproate on epileptiform activity. The study design was an in vivo animal experimental study. Forty Wistar-albino rats were divided into five groups, each with eight rats. The groups are categorized as the saline group, penicillin group (only penicillin), resveratrol group, sodium valproate group, and resveratrol + sodium valproate group. ECoG recording was taken for 180min in all groups and statistically evaluated. GABAα1, mGluR1/mGluR5, NMDAR1 receptor expressions in the hippocampus, and S100B level in serum were measured. The spike frequency decreased statistically to 60th min in the sodium valproate group and 150th min in the resveratrol group. The spike frequency decreased statistically in the 20th min and later measurements of the recording in the resveratrol + sodium valproate group. GABAα1 receptor expression was increased in all groups compared to the penicillin group. mGluR1/mGluR5, NMDAR1 receptor expression was decreased in all groups compared to the penicillin group. Serum S100B level increased in all groups compared to the penicillin group. There was no statistically significant difference in epileptiform activity when resveratrol alone was administered in the penicillin-induced epilepsy model. Resveratrol co-administered with sodium valproate significantly reduced epileptiform activity. Co-administration of the sodium valproate + resveratrol group made the receptor level's highest GABAα1receptor expression at receptors.

ОПРЕДЕЛЕНИЕ ЭФФЕКТИВНОЙ СХЕМЫ ЛЕЧЕНИЯ КОРОВ С СЕРОЗНО-ФИБРИНОЗНЫМ МАСТИТОМ

The purpose of the study is to determine the most effective treatment regimen for cows with serous-fibrinous mastitis. Objectives: conducting a comprehensive diagnosis of the cow population at the enterprise and identifying among them animals with clinical mastitis; comparison of treatment regimens for cows diagnosed with serous fibrinous mastitis; determining the most effective treatment regimen for the disease on the farm. Objects of study: cows, treatment regimens for mastitis. Research methods: clinical, laboratory studies of livestock and identification of animals with mastitis among them; conducting a comparative analysis of mastitis treatment regimens and determining the most effective one. At the enterprise, the cows were diagnosed with mastitis. 3 treatment regimens for serous-fibrinous mastitis were developed based on the sensitivity of the milk microflora in cows with mastitis to various antibiotics. In the 1st experimental group of animals, the treatment regimen was an antibiotic of the cephalosporin group – Ceftonit, Cobactan LC (1 syringe in the udder tank), NSAID – Flunex. In the 2nd – an antibiotic of the group of semi-synthetic penicillins – Amoxicillin, Gamaret (1 syringe in the udder tank), NSAIDs – Flunex. In the 3rd – a combination of an antibacterial drug of the tetracycline group – Nitox, Mastiet forte (1 syringe in the udder tank), NSAID – Flunex. As a result of comparing treatment regimens in three experimental groups, it was possible to identify the most effective one. When using the 3rd treatment regimen for cows, the most effective therapeutic effect was obtained, but in terms of the cost of drugs, the regimen turned out to be the most expensive. However, if we take into account the economic costs of milk loss while waiting for antibiotics to be removed from the cows’ bodies, the scheme is more effective.

Could ceftriaxone be a viable alternative to penicillin for the treatment of ocular syphilis?

This study was conducted to compare the effectiveness of ceftriaxone with that of aqueous crystalline penicillin G in treating ocular syphilis. We conducted a retrospective study from 2010 to 2021. Syphilis patients were administered either ceftriaxone (2 g intravenously daily for 14 days) or aqueous crystalline penicillin G [4 million units (MU) intravenously every 4 h for 14 days] as therapeutic interventions. Subsequently, we utilized these two groups to assess the serological results, cerebrospinal fluid analysis, and visual acuity at time intervals spanning 3 to 6 months post-treatment. A total of 205 patients were included, with 34 assigned to the ceftriaxone group and 171 to the penicillin group. The median age of patients was 56 years, with an interquartile range of 49-62 years, and 137 of them (66.8%) were male. Between 3 and 6 months after treatment, 13 patients (38.2%) in the ceftriaxone group and 82 patients (48.0%) in the penicillin group demonstrated effective treatment based on the clinical and laboratory parameters. The crude odds ratio (OR) was 0.672 (95% confidence interval [CI]: 0.316-1.428, P = 0.301), indicating no significant difference in effectiveness between the two groups. Thirty patients (17.5%) in the penicillin group and six patients (17.6%) in the ceftriaxone group did not experience successful outcomes. Notably, no serious adverse effects were reported in both the groups. There was no significant difference in the effectiveness of ceftriaxone and aqueous crystalline penicillin G in treating ocular syphilis. The administration of ceftriaxone without requiring hospitalization presents a convenient and safe alternative treatment option for ocular syphilis.

A Novel Approach for Single-Step Analyte Fractionation of Raw Milk Prior to Antibiotic Residue Trace Analysis as an Alternative to QuEchERS-Based Extraction.

Antibiotic residues in milk are a well-known hazard in the dairy food chain. Detection methods for these residues, such as nonspecific microbiological inhibitor tests or group-specific receptor tests, are relatively inexpensive, easy to use, and widely applied to ensure food safety. In contrast, specific detection by liquid chromatography-tandem mass spectrometry (LC-MS/MS)-although a critical, complimentary method to confirm the results of nonspecific testing-is relatively costly, time-consuming, and laborious. Furthermore, sample processing before LC-MS/MS analysis requires unique preparation procedures for different groups of antibiotic compounds. To simplify and speed up specific antibiotic residue detection, a low-cost, passive, and single-step method to fractionate analytes in raw milk was developed. Untreated raw milk was fractionated into its water and fat/protein phases using a Fractionation of Milk for Trace Analysis of Contaminants and Residues for Antibiotics (FraMiTrACR® AB) fractionation unit. The water fraction was then analyzed by LC-MS/MS. The analyte fractionation method was evaluated against a Quick Easy Cheap Effective Rugged and Safe (QuEChERS)-based method for sample preparation. Our method allows qualitative and quantitative detection of substances from the penicillin, cephalosporin, macrolide, lincosamide, sulfonamide, tetracycline, and fluoroquinolone groups of antibiotics. Detection limits are below the legally prescribed maximum residue levels, allowing reliable, specific, and rapid validation of a positive result in nonspecific microbiological inhibitor tests. Analyte fractionation by FraMiTrACR AB is a faster alternative to QuEChERS-based sample preparation for the detection of antibiotic substances in milk. This method describes a low-cost, environmentally friendly, passive, and single-step milk analyte fractionation. As an alternative to QuEChERS-based preparation, this fractionation method simplifies and speeds up the process for specific antibiotic residue detection.

ОБНАРУЖЕНИЕ АНТИБИОТИКОВ В МОЛОКЕ ПО ЕГО ФОТОЛЮМИНЕСЦЕНТНЫМ СВОЙСТВАМ

The use of antibiotics in the breeding of farm animals is an urgent problem with regard to food safety. For the dairy industry, the detection of residual amounts of antibiotics is crucial because of their inhibitory effect on fermentation processes. The most promising are optical screening methods for detecting these substances in milk and its processed products. Using optical sensors, it is possible to recognize a wide range of substances with high sensitivity and detect the residual content of various antibiotics, including the penicillin group. (Research Purpose) The research purpose is studying the possibility of detecting antibiotics of the penicillin group in milk by its photoluminescent properties. (Materials and methods) The spectral characteristics of milk excitation were measured on a CM 2203 spectrofluorimeter in the range from 200-500 nanometers. The control measurement of the antibiotic content in milk was carried out using BIOEASY 4in1 BSCT test kits. (Results and discussion) Four waves with a maximum length of 290, 324, 360 and 445 nanometers were present in the excitation spectrum of all milk samples. The spectra are measured and the integral fluxes of milk photoluminescence are calculated. (Conclusions) The presence of penicillin antibiotics in milk affects the optical properties of photoluminescence. The most informative are the excitation wavelength 360 and the photoluminescence detection range at 400-650 nanometers. The conducted studies confirm the possibility of qualitative determination of the presence of antibiotics in milk at a concentration of 0.001 mg/kg and above, which is 3-4 times more accurate than using the method with BIOEASY 4in1 BSCT test kits.

Mec Genes in Methicillin Resistant Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus (MRSA) manifests antibiotic resistance, especially for the β-lactams antibiotic group. MRSA bacteria are a common cause of infection in humans. The antibiotic resistance characteristic comes from the mecA, mecB, and mecC genes in the bacterial chromosome. mecA is the most common gene found in MRSA. Therefore, it is essential to know the role of the mecA gene in antibiotic resistance. This paper searched the literature about MRSA bacteria, the mec gene, and their relationship to cause resistance. The results showed that the mec gene found in MRSA bacteria causes antibiotic resistance in penicillin groups. Methicillin-resistant Staphylococcus aureus (MRSA) manifests antibiotic resistance, especially for the β-lactams antibiotic group. MRSA bacteria are a common cause of infection in humans. The antibiotic resistance characteristic comes from the mecA, mecB, and mecC genes in the bacterial chromosome. mecA is the most common gene found in MRSA. Therefore, it is essential to know the role of the mecA gene in antibiotic resistance. This paper searched literature about MRSA bacteria, the mec gene, and their relationship to cause resistance. The results showed that the mec gene found in MRSA bacteria causes antibiotic resistance in penicillin groups.

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Monitoring the Use of Antibiotics in Children Patients Acute Respiratory Infection Using ATC/DDD Method

The use of antibiotics needs to be monitored because it is known that excessive use of antibiotics can increase resistance in the community and this has become a focus both nationally and globally. This study aims to evaluate the use of antibiotics in patients with acute respiratory infection at Hasanuddin University Hospital using the ATC/ DDD analysis method. The research design used a cross-sectional study design with retrospective data collection. The sample in this study was patient medical record data from January-December 2021, taken from total sampling and met the inclusion and exclusion criteria. Descriptive statistics are used to present and analyze data. Evaluation of the use of antibiotics by quantitative methods ATC/DDD (Anatomical Therapeutic Chemical (ATC)/Defined Daily Dose (DDD), DDD/1000/ patient/day. The results showed that the most widely used antibiotic was ampicillin (280 mg) 32.96 DDD/1000 patients/day and the lowest was cefadroxil (150 mg) 1.45 DDD/1000 patients/day. Antibiotics with the highest DDD / 100-patient days value and exceeding the WHO DDD standard were ampicillin antibiotics from the penicillin group with a DDD / 100 patient-day value of 32.96 DDD /100 patient-days.

Спектрофотометрическое определение некоторых β-лактамных антибиотиков в их бинарных смесях с использованием метода проекций на латентные структуры

The method has been proposed for the spectrophotometric determination of antibiotics of the cephalosporin (cefuroxime, ceftriaxone, cefotaxime, cefazolin) and penicillin (amoxicillin) groups using chemometric data processing (method of partial least squares, PLS). The absorption spectra of 24 binary mixtures of “cephalosporin – cephalosporin” and 16 binary mixtures of “cephalosporin – amoxicillin” with various concentrations in the range of 3–27 and 5–140 μg/ml have been recorded. The optimal number of latent variables for each chemometric method (PLS-1 and PLS-2) has been selected, which ranged from 2 to 7 depending on the type of model and the nature of the antibiotics. The smallest root mean square calibration errors (RMSEC) have been found for the ceftriaxone–cefazolin system, which have been 0.09 and 0.08 for PLS-1 and PLS-2, respectively. The smallest root mean square errors of prediction (RMSEP) for the mixture “cefuroxime – amoxicillin” have been 0.07 for both methods (PLS-1 and PLS-2). It has been established that equations of linear relationships “measured-predicted” are characterized by a regression coefficient and the square of the approximation coefficient close to 1; which indicates the high quality of the models. The relative error of determination in test mixtures has been 0.4–6.2% by the PLS-1 method for “cephalosporin – cephalosporin” mixtures, and 0.07–6.0% by the PLS-2 method. At the same time, there is no significant difference in the error in determining the components of the mixture, in contrast to the “cefuroxime – amoxicillin” system, for which the error is 0.1–1.1% for the first component and 2.6–14.7% for the second component using the PLS-1 method and 0.2–1.2% for the first component and 4.3–13.3% for the second component using the PLS-2 method. Thus, it has been shown that the use of chemometric methods ensures high accuracy of the spectrophotometric determination of the studied antibiotics with overlapping absorption bands in their mixtures.

Augmentin loaded functionalized halloysite nanotubes: A sustainable emerging nanocarriers for biomedical applications

Clay minerals such as Halloysite nanotubes (HNTs), abundantly available green nanomaterial, exhibit a significant advantage in biomedical applications such as drug delivery, antibacterial and antimicrobials, tissue engineering or regeneration, etc. Because of the mesoporous structure and high absorbability, HNTs exhibit great potential as a nanocarrier in drug delivery applications. The sulfuric acid treatment enhances the surface area of the HNTs and thereby improves their drug-loading capacity by enlarging their lumen space/inner diameter. In the present investigation, based on the literature that supports the efficacy of drug loading after acid treatment, a dual treatment was performed to functionalize the HNTs surface. First, the HNTs were etched and functionalized using sulfuric acid. The acid-functionalized HNTs underwent another treatment using (3-aminopropyl) triethoxysilane (APTES) to better interact the drug molecules with the HNTs surfaces for efficient drug loading. Augmentin, a potential drug molecule of the penicillin group, was used for HNTs loading, and their antibacterial properties, cytotoxicity, and cumulative drug release (%) were evaluated. Different characterization techniques, such as X-ray diffractometer (XRD) and Fourier Transform Infra-Red (FT-IR), confirm the loading of Augmentin to the APTES@Acid HNTs. TEM images confirm the effective loading of the drug molecule with the HNTs. The drug encapsulation efficiency shows 40.89%, as confirmed by the Thermogravimetric Analysis (TGA). Also, the Augmentin-loaded APTES@Acid HNTs exhibited good antibacterial properties against E. coli and S. aureus and low cytotoxicity, as confirmed by the MTT assay. The drug release studies confirmed the sustainable release of Augmentin from the APTES@Acid HNTs. Hence, the treated HNTs can be considered as a potential nanocarrier for effectively delivering Augmentin and promoting enhanced therapeutic benefits.

Prevalence of Antibiotics Resistance among Patients in Iraqi Hospitals

Background: Antibiotics are medicines that fight bacterial infections. If they are used probably, they can save lives. Still there is a growing problem of antibiotic resistance. It happens when bacteria undergo some mutation and become able to resist the effect of an antibiotic. Objectives: This cross sectional study was to determine the prevalence of antibiotics resistance among group of patients and to know the causes that lead to antibiotic resistance crisis to compare the results with previous studies concerning this subject in order to identify the risks of antibiotic resistance to offer some solutions (if any) that can minimize this problem at Iraqi hospitals. Methodology: This cross sectional study was conducted from May to October 2019. The sample was 128 patients (their specimens were cultured and antibiotic sensitivity was tested) especially toward a group of antibiotic. Results: At the end of this study the result showed that a higher group of resistance especially to penicillin group and a lower rate to azithromycin, another finding was the bacteria which infect (admitted in patients) were resistant to multiple drugs than those which infect (out patients). Conclusion: A high resistance to penicillin group, Staphylococcus aureus was found to be the most resistant microorganism as culture results showed.

Peptide barcode of multidrug-resistant strains of Neisseria gonorrhoeae isolated from patients in Thailand.

The emergence of multidrug-resistant strains of Neisseria gonorrhoeae constitutes a serious threat to public health. The present study aimed to investigate peptidome-based biomarkers of multidrug-resistant N. gonorrhoeae, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography tandem mass spectrometry (LC-MS). The peptide barcode database of multidrug resistant N. gonorrhoeae was generated from the whole-cell peptides of 93 N. gonorrhoeae isolated from patients in Thailand. The dendrogram of 93 independent isolates of antibiotic-resistant N. gonorrhoeae revealed five distinct clusters including azithromycin resistance group (AZ), ciprofloxacin resistance group (C), ciprofloxacin and penicillin resistance group (CP), ciprofloxacin and tetracycline resistance group (CT), ciprofloxacin, penicillin and tetracycline resistance group (CPT). The peptidomes of all clusters were comparatively analyzed using a high-performance liquid chromatography-mass spectrometry method (LC-MS). Nine peptides derived from 9 proteins were highly expressed in AZ (p value < 0.05). These peptides also played a crucial role in numerous pathways and showed a strong relationship with the antibiotic resistances. In conclusion, this study showed a rapid screening of antibiotic-resistant N. gonorrhoeae using MALDI-TOF MS. Additionally, potential specific peptidome-based biomarker candidates for AZ, C, CP, CT and CPT-resistant N. gonorrhoeae were identified.

Therapeutic efficacy of the semi-synthetic penicillin group for intestinal infection in piglets

Therapeutic efficacy of the semi-synthetic penicillin group for intestinal infection in piglets