Patharnakh’ (PN) (Pyrus pyrifolia Burm. Nakai) and ‘Punjab Beauty’ (PB) [Pyrus communis L. × Pyrus pyrifolia Burm. Nakai] are the leading low-chill pear cultivars of the subtropics of India. Diurnal temperature and relative humidity during the fruit harvest period are high, which considerably affects the shelf life of fruits. Fruits of ‘PN’ and ‘PB’ pear harvested at physiological maturity were stored for 16 days at ambient temperature, and the effects of the storage day interval on physical and qualitative parameters were studied. Both cultivars showed reductions in fruit firmness, physiological weight loss, total soluble solids, and juice acid content during storage. Activities of fruit softening enzymes such as polygalacturonase (PG), pectin methylesterase (PME), and cellulase were enhanced, whereas those of superoxide dismutase (SOD) were reduced during storage. Fruit firmness was negatively correlated with polygalacturonase, pectin methylesterase, PPO, and cellulase enzymes in both cultivars. In both cultivars, the increased browning of the fruit during storage was negatively correlated with total phenolic content and positively correlated with polyphenol oxidase activity during storage. ‘Patharnakh’ and ‘Punjab Beauty’ fruits maintain desirable quality parameters up to 6–12 days and 4–8 days, respectively, during storage at ambient conditions.