Pearl Millet In India Research Articles

First report of Curvularia spicifera (≡ Bipolaris spicifera) causing spathe blight and leaf spot disease of pearl millet (Cenchrus americanus) in India.

Cenchrus americanus (L.) Morrone (Poaceae), is an important millet crop cultivated mainly in arid and semiarid regions and is a staple food grain for millions of people. During 2021 July surveys in the pearl millet fields in Mysore (12°30'55" N; 76°56'54" E), Karnataka, India, plants showed spathe blight and leaf spot disease with an overall incidence ranging from 5 - 8% in the 15 hectares surveyed. Infected leaves appeared brown, and lesions extended to the sheath. Some spathes were also found infected with similar symptoms. Diseased leaves and spathes were collected (n = 5 each) for pathogen identification. Samples were cut into small pieces (0.5 cm2), sterilized with sodium hypochlorite (2%, v/v), and blotted dried. The associated fungal pathogen was isolated on potato dextrose agar (PDA) medium amended with Streptomycin (40 mg/L) and incubated at 28 ºC for 1 week. Colonies were grey, fluffy, cottony with an irregular margin, undulate and dark brown in the back of the plate. Conidiophores were pale brown, erect, slightly curved, septate, unbranched, verruculose and measured 27.1 - 94 µm in length × 2.3 - 4.5 µm in width (n = 20). Conidiogenous cells were brown, subcylindrical, irregularly shaped, and conidia were straight, mainly elliptical, dark brown smooth, with two to three septa, with measurements of 11.1 - 26.4 µm by 5.7 - 14.3 µm (n = 50). Based on morphological characters, the pathogen was identified as Curvularia sp. Two representative isolates (UOMPM1 & UOMPM2) were molecularly identified. The total genomic DNA was extracted with a CTAB method, and ITS, GAPDH and tef-1α loci were amplified using primers ITS1/ITS4 (White et al., 1990), GPD1/GPD2 (Berbee et al., 1999) and EF1983F/EF-2218R (Schoch et al., 2009) respectively. ITS sequence had 100% similarity (706/706bp) with reference sequence C. spicifera (MH863648; HF934915 & HF934916); tef-1α sequence had 100% (933/933bp) identity with C. spicifera (KM062878, KJ939505), and the GAPDH sequence was 99.8% identical to that of Curvularia sp. (MG979055), and C. spicifera (MH809681). Combined dataset of concatenated sequence (ITS-GAPDH-tef-1α) was used in a phylogenetic analysis and revealed that the isolates were in a common clade with the isolate of Curvularia spicifera (CBS 274.52) thus, confirming the identity of the isolated pathogen as C. spicifera. The sequences obtained in the present study were deposited in the GenBank (ITS: OQ253406, OQ253407; LSU: OQ253429, OQ253430; GAPDH: OQ263372, OQ263373 & TEF: OQ263374, OQ263375). Pathogenicity test was carried out by inoculating (foliar /whole plant spray) 60 healthy pearl millet plants (45-days old), grown in field plot with spore suspension (105 conidia/ml). Control plants (n=20) were treated with sterile water. The experiments were conducted in triplicates and repeated twice. Development of disease symptoms was recorded on 41 plants, and all control plants remained healthy. The identity was confirmed after re-isolation as C. spicifera based on cultural and molecular sequence analysis. To our knowledge, this is the first report of C. spicifera causing a leaf spot and spathe blight disease of pearl millet in India. This disease seriously affects grain production, and effective disease management strategies need to be investigated.

Characterization of the Pearl Millet Cultivation Environments in India: Status and Perspectives Enabled by Expanded Data Analytics and Digital Tools

The cultivation of pearl millet in India is experiencing important transformations. Here, we propose a new characterization of the pearl millet production environment using the latest available district level data (1998–2017), principal component analysis, and large-scale crop model simulations. Pearl millet cultivation environment can be divided in up to five environments (TPEs). The eastern part of the country (Rajasthan, Haryana, Uttar Pradesh) emerges as the only region where pearl millet cultivation has grown (+0.4 Kha/year), with important yield increase (+51 kg/ha/year), and potential surplus that are likely exported. Important reductions of pearl millet cultivated area in Gujarat (−4.5 Kha/year), Maharashtra and Karnataka (−4 Kha/year) are potentially due to economy-driven transition to other more profitable crops, such as cotton or maize. The potential rain increase could also accelerate this transition. With R2∈ [0.15–0.61], the tested crop models reflected reasonably well the pearl millet production system in the A1 (North Radjasthan) and AE1 (South Rajastan and Haryana) TPEs covering the largest area (66%) and production share (59%), especially after the use of a new strategy for environment and management parameters calibration. Those results set the base for in silico system design and optimization in future climatic scenarios.

Use of balanced fertilization for achieving higher productivity of pearl millet and soil health under pearl millet based cropping systems

In India, Pearl millet grown in 7.41 million ha area with an average production of 10.3 million tonnes. Analysis of 1030 soil samples from major pearl millet growing areas representing 10 districts indicated widespread multi-nutrient deficiency involving 2 to 6 nutrients including NK, NKS, NKB, NPKS, NKSZn and NPKSZnB. Incidence and expansion of such multi-nutrient deficiencies in Indian soils owing to inadequate and unbalanced nutrient input through fertilizers is considered one of the major constraints in enhancing productivity of pearl millet. In this context, different nutrient supply options were evaluated with pearl milet-based cropping systems. In all these experiments, SSNM proved superior to state recommendation and farmers’ fertilizer practice in terms of annual pearl millet yields, nutrient recovery, soil fertility restoration and net economic returns. Site-specific nutrient management (SSNM) seems to be one of effective ways to improve nutrient supplying capacity of soil and enhance the productivity of pearl millet in India.&nbsp

How can machine learning help in understanding the impact of climate change on crop yields?

Ordinary least squares linear regression (LR) has long been a popular choice among researchers interested in using historical data for estimating crop yield response to climate change. Today, the rapidly growing field of machine learning (ML) offers a wide range of advanced statistical tools that are increasingly being used for more accurate estimates of this relationship. This study compares LR to a popular ML technique called boosted regression trees (BRTs). We find that BRTs provide a significantly better prediction accuracy compared to various LR specifications, including those fitting quadratic and piece-wise linear functions. BRTs are also able to identify break points where the relationship between climate and yield undergoes significant shifts (for example, increasing yields with precipitation followed by a plateauing of the relationship beyond a certain point). Tests we performed with synthetically simulated climate and crop yield data showed that BRTs can automatically account for not only spatial variation in climate–yield relationships, but also interactions between different variables that affect crop yields. We then used both statistical techniques to estimate the influence of historical climate change on rice, wheat, and pearl millet in India. BRTs predicted a considerably smaller negative impact compared to LR. This may be an artifact of BRTs conflating time and climate variables, signaling a potential weakness of models with excessively flexible functional forms for inferring climate impacts on agriculture. Our findings thus suggest caution while interpreting the results from single-model analyses, especially in regions with highly varied climate and agricultural practices.

Morphology and Influence of Abiotic Factors on Magnaporthe grisea

The present study entitled “Studies on Magnaporthe grisea incitant of blast disease of pearl millet [Pennisetum glaucum (L.) R. Br.]” was conducted during the kharif 2019 at research farm, Department of Plant Pathology, CCS Haryana Agricultural University, Hisar. Pearl millet blast disease is a devastating fungal disease causing considerable yield losses. Blast of pearl millet incited by Magnaporthe grisea is the most widespread and destructive disease of pearl millet in India and other pearl millet growing area of the world. This disease is a major factor limiting full exploitation of high yield potential hybrids in India. In the present investigation, culture medium plays an important role in growth and sporulation of fungus. Among 5 media tested, rice meal agar was found most effective for all the 3 isolates of pearl millet blast were tested. Combined effect of temperature and relative humidity also play an important role in growth and spore germination of fungus. Among five combination of temperature and relative humidity, 30℃ temperature with 100 % relative humidity was found most effective for spore germination.

Multi-location evaluation of fungicides for managing blast (Magnaporthe grisea) disease of forage pearl millet in India

Blast caused by Magnaporthe grisea has emerged as a major threat in recent years to forage pearl millet in India. Due to lack of resistant cultivars to blast disease, the disease can be best managed through fungicides. However, no comprehensive multi-location study in managing blast disease in forage pearl millet has been conducted in India. Therefore, field trials for two consecutive years (2019 and 2020) were conducted at four geographically different locations (Ludhiana, Palampur, Jhansi and Bhubaneswar) of India. Carbendazim, tebuconazole + trifloxystrobin, tricyclazole, chitosan and neem oil were tested for their efficacy through seed treatment alone or seed treatment followed by two foliar sprays at 15 days interval. Results showed the superiority of tebuconazole + trifloxystrobin as seed treatment followed by two foliar sprays in reducing the blast disease area under disease progress as well as rate of infection at Ludhiana, Jhansi and Palampur. Tricyclazole as seed treatment followed by two foliar sprays was found superior over others in reducing the area under disease progress curve as well as rate of infection at Bhubaneswar. Reduction in blast disease area under disease progress curve and rate of infection by applying these treatments helped in achieving a significant increase in the green fodder yield at the tested locations. Through this study, an effective strategy for location specific management of blast disease of forage pearl millet has been formulated.

Economics of Biofuel Production: A Case of Sorghum and Pearl Millet in India.

Reduction of fossil fuels at an alarming rate has attracted increasing attention to blending biofuels worldwide. India's energy demand is expected to grow at an annual rate of 4-5 times over the next couple of decades. With self-sufficiency levels in crude oil becoming a distant dream, there is growing interest to look out for alternative fuels and the biofuels are an important option for policy makers in India. In this context, this paper reviews the experiences in India in the last two decades with respect to biofuel cultivation and its impact on land use, environment, and the livelihoods of rural communities. The objective of this paper is to assess the economics of biofuel production using Sorghum and Pearl millet feedstocks in India using a Life Cycle Analysis (LCA) approach. Baseline study was conducted during the year 2013 in the Madhya Pradesh state of India covering five districts and 333 sample farmers to understand the farmers perception about the various issues related to the production of biofuels using Indian staple food crops Sorghum and Pearl millet. Empirical data from the multi-locational trials conducted during the years 2014-2015 and 2015-2016 in farmers' fields was used to conduct the LCA analysis. Sorghum and Pearl millet feedstocks which are rain-fed crops are considered for bioethanol production with different pretreatment methods. Net Energy Ratio (NER), Net Energy Balance (NEB), Net Carbon Balance (NCB), and % Carbon reduction were some of the key parameters used for analysis and the results are evaluated based on the environmental impacts through the Life Cycle Assessment at 5% blending. Findings reveal that, dilute alkali pretreatment process is most energy intensive due to consumption of alkali consumption. Whereas dilute acid pretreatment has higher conversion efficiency than the other pretreatment processes which is due to higher glucan and xylan conversion efficiencies.The study concludes that Sorghum feedstock is more energy intensive than Pearl millet feedstock due to higher water requirement and yield. Biofuels, either conventional or advanced, should not been couraged without a comprehensive outlook on the overall impact that will ultimately have on the society, environment, or on the countries' energy security. Efforts should be made toward encouragement of research and development in the field as well as in formulating a comprehensive and effective biofuel policy for India.

Pathogenic variability amongst Indian isolates of Magnaporthe grisea causing blast in pearl millet

Blast disease caused by Magnaporthe grisea is a major biotic constraint to the production of pearl millet in India. The pathogen is highly variable in nature, but isolates are mostly specific in their host range. Pathogenic variation among 80 M. grisea isolates collected from major pearl millet growing states in India was studied on pearl millet host differentials (ICMB 93333-P1, ICMB 95444, ICMB 97222-P1, ICMB 01333, ICMB 02444, ICMR 06444, 863B-P2, ICMR 06222, ICMR 11003 and IP 21187-P1) under greenhouse conditions. Based on reaction of isolates on host differentials (virulent = score ≥ 4 and avirulent = score ≤ 3 on a 1-to-9 scale), 80 isolates were clustered into 14 pathogenic groups. Based on the virulence profiling, isolates Pg 138, Pg 186, Pg 204, and Pg 232 representing different pathogenic groups, and collected from different states have been selected for greenhouse screening of pearl millet lines for blast resistance.

Production, returns & policy initiatives for pearlmillet in India

Production, returns & policy initiatives for pearlmillet in India

Markets, institutions and policies: A perspective on the adoption of agricultural innovations

Successful adoption of agricultural innovations depends not just on the right technology but also on markets, institutions, and policies. We illustrate this argument with four case studies of agricultural innovations in the semi-arid tropics, two with high and two with low adoption. We show that the success of both hybrid pearl millet in India and dual-purpose cowpea in Nigeria depended on identifying market demand correctly and on innovative institutions to overcome constraints in the production and delivery of improved seed. Conversely, the low adoption of improved varieties of pigeon pea in Malawi and conservation agriculture in Zimbabwe reflect uncertain market conditions, misunderstood demand and the lack of sustainable institutions for input delivery. The results highlight how variations in the enabling conditions may influence the fate of agricultural innovations.

Market integration of pearl millet in India

Market integration of pearl millet in India

Draft genome sequence of Sclerospora graminicola, the pearl millet downy mildew pathogen

Sclerospora graminicola pathogen is the most important biotic production constraints of pearl millet in India, Africa and other parts of the world. We report a de novo whole genome assembly and analysis of pathotype 1, one of the most virulent pathotypes of S. graminicola from India. The draft genome assembly contained 299,901,251bp with 65,404 genes. This study may help understand the evolutionary pattern of pathogen and aid elucidation of effector evolution for devising effective durable resistance breeding strategies in pearl millet.

Biofortification in Millets: A Sustainable Approach for Nutritional Security.

Nutritional insecurity is a major threat to the world’s population that is highly dependent on cereals-based diet, deficient in micronutrients. Next to cereals, millets are the primary sources of energy in the semi-arid tropics and drought-prone regions of Asia and Africa. Millets are nutritionally superior as their grains contain high amount of proteins, essential amino acids, minerals, and vitamins. Biofortification of staple crops is proved to be an economically feasible approach to combat micronutrient malnutrition. HarvestPlus group realized the importance of millet biofortification and released conventionally bred high iron pearl millet in India to tackle iron deficiency. Molecular basis of waxy starch has been identified in foxtail millet, proso millet, and barnyard millet to facilitate their use in infant foods. With close genetic-relatedness to cereals, comparative genomics has helped in deciphering quantitative trait loci and genes linked to protein quality in finger millet. Recently, transgenic expression of zinc transporters resulted in the development of high grain zinc while transcriptomics revealed various calcium sensor genes involved in uptake, translocation, and accumulation of calcium in finger millet. Biofortification in millets is still limited by the presence of antinutrients like phytic acid, polyphenols, and tannins. RNA interference and genome editing tools [zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)] needs to be employed to reduce these antinutrients. In this review paper, we discuss the strategies to accelerate biofortification in millets by summarizing the opportunities and challenges to increase the bioavailability of macro and micronutrients.

Assessment of Diversity in Commercial Hybrids of Pearl Millet in India

Availability of diverse cultivars is essential in order to meet necessity of regional adaptation to various climatic conditions and to fulfil the farmers’ need of differential preference of various phenotypic traits in pearl millet. The present study attempted to quantify the degree of diversity in commercial hybrids of pearl millet and to understand the relationship among various phenotypic and quality traits. A total of 122 commercial hybrids were evaluated at three diverse locations. Results showed large variation for flowering time (42-58 days), tillering (1.1-4.4 panicles/plant), individual grain size (7.6-17.3 mg), plant height (185-268 cm), panicle length (20-33 cm) and grain yield (35-72 q/ha). Clustering of hybrids resulted in 7 distinct clusters, which highlighted the successful efforts of national programme of pearl millet improvement toward genetic diversification of hybrids. These results are discussed as to how this reported diversity has helped in extending the hybrid technology in some of the most difficult and diverse production ecologies. Phenology, tillering and height had significant influence on yields of hybrids. Taller hybrids provided higher yield. Hybrids with lesser duration and shorter height produced more tillers and tillering decreased with the increase in panicle length. The correlation of grain yield with Fe and Zn concentration in grains was not significant suggesting that both these micronutrients can be improved without significantly compromising on grain yield.

Relating Iron Intake to Iron Status and Cognitive Functioning, Results from a 6-month Efficacy Trial Involving Biofortified Pearl Millet in India

Relating Iron Intake to Iron Status and Cognitive Functioning, Results from a 6-month Efficacy Trial Involving Biofortified Pearl Millet in India

Genetic Improvement of Pearl Millet in India

Pearl millet [Pennisetum glaucum (L.) R. Br.] is grown widely in the arid and semi-arid tropical regions in Indian subcontinent and African continent under the most adverse agro-climatic conditions where other crops like sorghum and maize fail to produce economic yields. Its grains are valued as human food while its dry stover makes important livestock ration in crop–livestock farming system. Enormous progress has been made in the genetic improvement of pearl millet in India during last several decades. This paper presents an overview of strategies followed in genetic improvement of pearl millet, assesses the impact of this research on crop productivity and presents its future prospects in climate-change scenario for providing food and nutritional security. The genetic improvement programme evolved strongly starting from selection in local and traditional material and reaching development of high-yielding hybrids with in-built resistance to diseases and tolerance to climatic stresses like drought and heat. The major approach in hybrid breeding has been to strategically utilize germplasm from Africa and Indian subcontinent with the result that a large number of genetically diverse hybrids have been developed with different combinations of phenotypic traits that are important for adaptation to different ecological regions. The genetic diversification of hybrids has proved very critical to contain downy mildew epidemics which had threatened hybrid technology per se in mid-1970s. A great deal of work has been done on understanding the epidemiology of different diseases and crop response to moisture stress that helped in developing disease-resistant and stress-tolerant cultivars. More than 100 cultivars with a combination of diverse phenotypic traits have been released in the last 25 years, providing a wide range of choice to farmers in different production ecologies of the crop. These cultivars have been widely adopted by Indian farmers with the result that the crop productivity has gone up from 305 kg ha−1 during 1951–1955 to 998 kg ha−1 during 2008–2012, registering a 227 % improvement which assumes greater significance given that more than 90 % of pearl millet is grown as rainfed and often on marginal lands. In future, pearl millet is likely to play a greater role in providing food and nutritional security. Pearl millet would also be an excellent genomic resource for isolation of candidate genes responsible for tolerance to climatic and edaphic stresses for accelerating further genetic improvement of this crop as well as for their possible deployment in the genetic improvement of other crops.

Pathogenic Variation in the Pearl Millet Blast Pathogen Magnaporthe grisea and Identification of Resistance to Diverse Pathotypes.

Blast, also known as leaf spot, caused by Pyricularia grisea (teleomorph: Magnaporthe grisea), has emerged as a serious disease affecting both forage and grain production in pearl millet in India. Pathogenic variation was studied in a greenhouse using 25 M. grisea isolates collected from four major pearl-millet-growing states in India (Rajasthan, Haryana, Maharashtra, and Uttar Pradesh) on 10 pearl millet genotypes (ICMB 02444, ICMB 02777, ICMB 06444, ICMB 93333, ICMB 96666, ICMB 97222, ICMB 99444, 863B, ICMR 06222, and ICMB 95444). Differential reactions to the test isolates were recorded on ICMB 02444, ICMB 93333, ICMB 97222, 863B, and ICMR 06222. The 25 isolates were grouped into five different pathotypes based on their reaction types (virulent = score ≥ 4 and avirulent = score ≤ 3 on a 1-to-9 scale). For the identification of resistance sources, a pearl millet mini-core comprising 238 accessions was evaluated under greenhouse conditions against five M. grisea isolates (Pg118, Pg119, Pg56, Pg53, and Pg45) representing the five pathotypes. Of 238 accessions, 32 were found to be resistant to at least one pathotype. Resistance to multiple pathotypes (two or more) was recorded in several accessions, while three accessions (IP 7846, IP 11036, and IP 21187) exhibited resistance to four of the five pathotypes. Four early-flowering (≤50 days) blast-resistant mini-core accessions (IP 7846, IP 4291, IP 15256, and IP 22449) and four accessions (IP 5964, IP 11010, IP 13636, and IP 20577) having high scores (≥7) for grain and green fodder yield potential and overall plant aspect were found to be promising for utilization in pearl millet improvement programs. Identification of five pathotypes of M. grisea and sources of resistance to these pathotypes will provide a foundation for breeding for blast resistance in pearl millet in India.

Inheritance of foliar blast resistance in pearl millet (Pennisetum glaucum)

With 1 tableAbstractFoliar blast, caused by Pyricularia grisea (Cooke) Sacc, has recently emerged as a serious disease of pearl millet in India. To study the inheritance of resistance to this disease, two resistant restorer lines (ICMR 06222 and ICMR 07555) and two susceptible maintainer lines (ICMB 95444 and ICMB 89111) were selected on the basis of foliar blast reaction in tests conducted under field and greenhouse conditions. Each of the two resistant parents was crossed with two susceptible parents to generate four sets of F1s, F2s and their backcrosses with both resistant and susceptible parental lines. These were evaluated for disease reaction with artificial inoculation under both field and greenhouse conditions. The disease reaction of the F1s, and the segregation patterns of resistance in the F2s and backcross generations, showed that resistance to foliar blast in pearl millet is controlled by a single dominant gene.

Influence of dosage, storage time and temperature on efficacy of metalaxyl-treated seed for the control of pearl millet downy mildew

Metalaxyl (Apron 35WS) as a seed treatment has been used extensively to control downy mildew (caused by Sclerospora graminicola) in pearl millet in India. However, the extent of disease control has varied across cultivars, years and locations. We investigated the effects of fungicide dosage, storage time and storage temperature of metalaxyl-treated seed on disease incidence in four pearl millet lines having varying levels of resistance. A linear relationship was found between fungicide dosage (0.5, 1.5 and 2 g a.i. kg−1 seed) and reduction in disease incidence up to 40 days after emergence in all the lines. The normal fungicide dose (2 g a.i. kg−1 seed) protected the crop for up to 20, 40 and 50 days after emergence in highly susceptible (7042S), moderately susceptible (4042R), and moderately resistant (ICMP 451) lines, respectively. However, the quarter and half the normal dosage of fungicide provided protection only up to 20 days after emergence in 7042R and 40 days after emergence in ICMP 451. Storage duration of metalaxyl-treated seed (2 g a.i. kg−1) up to 9 months at 25 ± 2°C did not affect fungicide efficacy. Storage temperatures (5, 25 and 40°C) and duration (30, 60 and 90 days) of metalaxyl-treated seed (2 g a.i. kg−1) showed differential effects in two pearl millet lines 7042S and 843B with downy mildew incidence being significantly lower in 7042S than in 843B. Metalaxyl-treated seed of 7042S and 843B stored at 40°C for different durations showed phytotoxic effects and it was more pronounced in 843B stored for 60 and 90 days where seed germination was inhibited in pot soil.

The impact of social networks on hybrid seed adoption in India

AbstractThis article adds to the literature about the impact of social networks on the adoption of modern seed technologies among smallholder farmers in developing countries. The analysis centers on the adoption of hybrid wheat and hybrid pearl millet in India. In the local context, both crops are cultivated mainly on a subsistence basis, and they provide examples of hybrid technologies at very different diffusion stages: while hybrid wheat was commercialized in India only in 2001, hybrid pearl millet was launched in 1965. The analysis is based on surveys of wheat and millet farmers in the state of Maharashtra. Comprehensive data on farmer characteristics and social interactions allow for identifying individual networks, thereby improving upon previous research approaches that employed village‐level variables as proxies for network effects. Using econometric models, we find that individual social networks play an important role for technology adoption decisions. While village‐level variables may be used as suitable proxies at later diffusion stages, they tend to underestimate the role of individual networks during early phases of adoption.