Peanut Milk Research Articles

Effects of germinated and ungerminated grains on the production of non-dairy probiotic-fermented beverages

Non-dairy probiotic-fermented beverages were produced from germinated and ungerminated barley, highland barley and rice, which were germinated for 48–96 h at 30ºC, then dried, baked, ground, mixed and blended in a ratio of 3:2:1. Varying amounts of the mixture (0, 2, 4, 6, and 8 g) were added to soybean milk, peanut milk or coconut milk. Distilled water was used as a control. Sucrose (4 g) was added to each solution, followed by inoculation with Lactobacillus plantarum strain ZJ5 for 6 h. After fermentation, the pH, acidity, bacterial counts and antioxidant activity were measured and sensory evaluations were performed. Increase in the concentration of grain mixture to 8 g increased the pH, acidity, probiotic count, antioxidant content and polyphenol content values as well. The antioxidant capacity of germinated and ungerminated grain probiotic beverages was 1.08–1.26 mM and 0.69–1.08 mM, while the total phenolic content was 1.10–3.91 mM and 1.03–2.79 mM, respectively. The sensory evaluation scores of coconut beverages were higher than those of other beverages. Probiotic beverages containing 6 g of cereal mixture had the highest acceptability scores. Beverages containing germinated, as opposed to ungerminated, grains had higher nutritional value and better sensory qualities. The results highlighted the importance of germinated grains to improve the quality of non-dairy probiotic-fermented beverages.

In vitro antioxidant, anti-glycation, and bile acid-lowering capacity of peanut milk fermented with Lactiplantibacillus plantarum Kinko-SU4

Plant-based milk-like products from soybeans and other legumes and nuts have been explored worldwide, owing to their nutritional and functional characteristics. This study was conducted to develop new functional food materials from peanut (Arachis hypogaea) milk (PM) with desirable health functions to mitigate lifestyle and age-related diseases. The antioxidant, anti-glycation and bile acid-lowering properties of PM fermented with lactic acid bacteria Lactiplantibacillus plantarum Kinko-SU4 (FPM) were determined in vitro. L. plantarum Kinko-SU4 lowered the pH level from 6.4 to 4.3, 3.9, and 3.7 at 10, 24, and 48 h, respectively. The lactic acid concentration was 4.4 mg/mL after 48 h of incubation. The starter degraded the dissolved proteins in PM, including Ara h 1, one of the peanut allergens. Although the total phenolic content was 36% lower in FPM than in unfermented PM, O2− radical-scavenging capacity was high in FPM. Anti-glycation in a bovine serum albumin-fructose model and the bile acid-lowering capacities of PM were distinctly increased following fermentation. The result of this study infers that PM fermented with L. plantarum Kinko-SU4 can be considered a desirable food material to prevent and ameliorate chronic lifestyle diseases, particularly in the elderly.

우유 대체 식물성 기반 우유 유사체 개발에 관한 현황과 미래

Following the COVID-19 pandemic, many people are increasingly becoming interested in health and environmental issues. Therefore, the sale of vegan or vegetarian products has been increasing over the last few years, as well as interest in non-dairy plant-based milk that can replace cow’s milk. Furthermore, the global food industry has developed an interest in such products, considering the recent changes in consumer trends. In Korea, various products are being launched annually due to the increasing interest in non-dairy plant-based milk. However, research with regard to the quality and type of products produced in Korea is still at the preliminary stage when compared to those in the United States and Europe. Therefore, the present review has summarized non-dairy plant-based milk analogues based on the following key aspects. First, the types of non-dairy plant-based milk analogues and their production technologies (in the order of almond milk > cocoa milk > coconut milk > hemp milk > kidney bean milk > oat milk > peanut milk > rice milk, and soy milk). Second, the current status and future prospects for non-dairy plant-based milk analogues. Third, recent trends and future challenges associated with the production and quality improvement of non-dairy plant-based milk analogues. Fourth, the current status and outlook of the non-dairy plant-based milk analogue market in Korea. In conclusion, the present review could provide the food industry with valuable information regarding non-dairy plant-based milk analogues to facilitate the development of related products. Data were obtained from previously published studies.

Impact of Different Processing Methods on Physico-chemical Characteristics and Antioxidant Activity of Peanut Milk

Background: Peanut milk was prepared by different processing methods like fresh, blanching, soaking, roasting and germination methods and analyzed its physico chemical properties like pH, TSS, acidity, viscosity and colour value and also analyzed its antioxidant activity in both the selected local and CO 6 peanut varieties and studied the effect of processing methods on physico chemical properties, organoleptic characteristics and antioxidant activity. Methods: Local and CO 6 peanut variety were selected for preparation of peanut milk by five different processing methods like fresh, blanching (121°C 15 psi for 2 mins), soaking for 3 hrs, roasting (roasting for 5 mins followed by soaking 3 hrs) and germination (8 hrs) pretreatments were done. Then grinded (1:3 ratio of kernel to water), filtered by using muslin cloth, slurry was separated. Then the peanut milk was homogenized for 5 mins and double pasteurized (85°C for 15 mins). Result: The present study results reported that physico chemical properties of peanut milk (local and CO 6 variety) pH value varied between 6.3-6.9, TSS 11-13°Bx, viscosity 4.80-5.00 (Cp/100 ml), acidity 0.04-0.08 (g%), colour value L*a*b was higher (91.02, 0.97, 12.47) in control peanut milk from both the local and CO 6 variety respectively. Antioxidant activity was significantly higher in peanut milk developed from roasting method (roasting for 5 mins followed by soaking 3 hrs) in both the peanut varieties. Antioxidant activity of the peanut milk is also important for maintaining the shelf life of the product and prevent oxidative damage in human being.

Characterization and stability of peppermint oil emulsions using polyglycerol esters of fatty acids and milk proteins as emulsifiers.

Three peppermint oil emulsions using polyglycerol esters of fatty acids-casein (PGFE-CN), polyglycerol esters of fatty acids-sodium caseinate (PGFE-NaCN), and polyglycerol esters of fatty acids-whey protein isolate (PGFE-WPI) as emulsifiers were fabricated, and the droplet size, zeta potential, viscosity, and stability of emulsions were determined. The experimental results showed that the emulsion containing PGFE-CN has relatively smaller droplet size of 231.77± 0.49nm. No significant changes were observed on the average particle size, polydispersity index and zeta potential during 4-week of storage, indicating that the emulsions kept stable against pH, salt ion, freeze-thaw, and storage. Fourier transform infrared spectrometer (FTIR) results showed that the electrostatic interaction occurs between CN and PGFE in the emulsion. The confocal laser scanning microscope (CLSM) was used to observe the microstructure of the emulsion, proving that droplets were evenly distributed throughout the aqueous phase by PGFE-CN emulsifier. The protein-stabilized emulsions can be used as potential carriers for the delivery of the lipophilic nutrients such as peppermint oil. PRACTICAL APPLICATION: PGFE-CN emulsifier can be directly added to the beverage systems containing oil or protein, such as coconut milk, peanut milk, and walnut milk. It can enhance the stability of beverage, prevent the precipitation, stratification, and oil floating, improve the homogeneity of the system and therefore extend the shelf life.

Study of Making Yogurt From Peanut (Arachis hypogaea L.) With The Addition of Coconut Water and Skim Milk

Abstract- Plant-based functional foods has been highly developed in recent years. Peanut (Arachis hypogaea L.) can be used as functional drink that substitute for cow's milk. This study aimed to determine the effect of ratio (peanut milk and coconut water) and skim milk concentration on the quality of peanut yogurt. Yogurt was made by mixing peanut milk with coconut water and adding skim milk. The ratio of peanut milk and coconut water (50% : 50% and 75% : 25% v/v) and skim milk concentration (0%, 1% and 2% w/v). Fermentation was carried out at room temperature for 12 hours using commercial yogurt starter culture. Peanut yogurt analysis included of total LAB, pH, total acid and organoleptic. The data obtained were tabulated and presented descriptively. The best peanut yogurt was obtained in the ratio of peanut milk and coconut water (75%: 25%) and the addition of 2% skim milk which showed total LAB, pH and total acid were 9.2 x 109 CFU/mL, 2.8 and 0.37% respectively.

Amino acid-enriched plant-based RUTF treatment was not inferior to peanut-milk RUTF treatment in restoring plasma amino acid levels among patients with oedematous or non-oedematous malnutrition

Ready-to-use therapeutic food (RUTF) with adequate quality protein is used to treat children with oedematous and non-oedematous severe acute malnutrition (SAM). The plasma amino acid (AA) profile reflects the protein nutritional status; hence, its assessment during SAM treatment is useful in evaluating AA delivery from RUTFs. The objective was to evaluate the plasma AAs during the treatment of oedematous and non-oedematous SAM in community-based management of acute malnutrition (CMAM) using amino acid-enriched plant-based RUTFs with 10% milk (MSMS-RUTF) or without milk (FSMS-RUTF) compared to peanut milk RUTF (PM-RUTF). Plasma AA was measured in a non-blinded, 3-arm, parallel-group, simple randomized controlled trial conducted in Malawi. The RUTFs used for SAM were FSMS-RUTF, MSMS-RUTF or PM-RUTF. A non-inferiority hypothesis was tested to compare plasma AA levels from patients treated with FSMS-RUTF or MSMS-RUTF with those from patients treated with PM-RUTF at discharge. For both types of SAM, FSMS-RUTF and MSMS-RUTF treatments were non-inferior to the PM-RUTF treatment in restoration of the EAA and cystine except that for FSMS-RUTF, methionine and tryptophan partially satisfied the non-inferiority criteria in the oedematous group. Amino-acid-enriched milk-free plant-source-protein RUTF has the potential to restore all the EAA, but it is possible that enrichment with amino acids may require more methionine and tryptophan for oedematous children.

TMT-labeled quantitative proteomic analysis to identify proteins associated with the stability of peanut milk.

Peanut milk benefits human health mainly due to its high protein content and suitable amino acid composition. To reveal the molecular mechanism affecting the quality of peanut milk, tandem mass tag (TMT)-labeled proteomic analysis was applied to identify the proteome variation between two peanut cultivars that produced peanut milk with the best and worst stability. A total of 478 differentially abundant proteins (fold change >1.2 or <0.83, P < 0.05) were identified. Most of these proteins were located in the cytoplasm and chloroplasts. Correlation analysis showed that RNA recognition motif (RRM) domain-containing protein (17.1kDa) had a negative relationship with the sedimentation rate of peanut milk and that 22.0kDa class IV heat shock protein was negatively correlated with the creaming index (P < 0.05). Bioinformatic analysis showed that the molecular function of RRM domain-containing protein (17.1kDa) was associated with RNA binding and nucleotide binding, and 22.0kDa class IV heat shock protein was involved in the pathway of protein processing in the endoplasmic reticulum. Overall, the differentially abundant proteins in the biological metabolic pathway might offer some potential markers to guide future peanut breeding, especially for the production of peanut milk. © 2021 Society of Chemical Industry.

Drying kinetics and quality of tray dried peanut milk residue

The research was aimed to prepare muffins using groundnut milk residue. Groundnut or Peanut (Arachis hypogaea) is one of the most consumed legumes in the world. Groundnut is also a prominent source of plant-based milk. The groundnut milk was prepared by the conventional method with 1:2 ratio of water. The residue was tray-dried at different temperatures (60, 70, 80°C) to study the drying kinetics. Physical and chemical properties of optimized groundnut milk residue powder were analysed. Muffins were developed using milk residue. Quality parameters and sensory analyses were also done for the product obtained from milk residue. The results indicated that groundnut milk residue dried at 70ºC had less water absorption capacity and more water solubility index. Also, the powder retained colour when dried at 70°C. The experimental data were fitted to five mathematical models. Wang and Singh's model was found to have the best fit with R2 value of 0.9932. The increase in temperature promotes drying at a faster rate.

Optimization of nondairy curd using response surface methodology

Bambusa arundinacea, being grown naturally in a forest is wild in nature. Bambusa arundinaceaseeds are collected from forest areas and being sold as Bamboo rice which is highly nutritious andencompasses many medicinal properties. Ingestion of proteins present in dairy milk is capable ofcausing cow’s milk allergy. An elimination diet is a dietary protocol adopted to overcome this issue byexcluding protein from dairy foods. As a replacement for the missed out protein, the current studyfocused to formulate a Non-dairy curd using a suitable plant-based starter. The primary objective of theexploration is to amend the process and ingredients used to formulate a non-dairy curd. A quadraticmodel was executed to inter-relate the independent variable[Bamboo rice milk[X,ml]and Peanutmilk[X,ml] ] towards the response Titrable acidity [Y1, %], Total solids [Y2, %], and pH [Y3] for theperfect development of the curd by Central Composite Design as a tool. The R2 value of the responseswere significant with the high-level degree of 0.9365, 0.9610, and 0.8710 respectively. Independentvariables had persuasive results on the dependent variables [p is less than 0.05] which matched thestatistical model. The results of the optimized combination of process ingredients were 79.9 ml Bamboorice milk, 31.57ml peanut milk while the optimal response values of Titratable acidity, Total solids, andpH were 0.106%, 7.73%and 4.52 with the highest desirability index of 1 respectively. Hence the studytherefore concluded that RSM was successfully adopted for the optimal level of plant-based curdproduction ingredients.

EFFECT OF DIFFERENT PROCESSING METHODS ON THE NUTRITIONAL COMPOSITION AND ANTINUTRITIONAL FACTORS OF PEANUT MILK

Comparative effects of different processing methods like blanching, soaking, roasting and germination methods on nutritional composition and antinutritional factors in peanut milk were investigated. Local and CO 6 peanut varieties were selected for peanut milk preparation and subjected to different processing methods. Peanut milk was extracted from fresh (control), blanched (2 mins), soaked (3 hrs), roasted (roasting 5 mins followed by soaking 3 hrs) and germinated (8 hrs) peanuts. Peanuts were washed, ground, slurry separated, filtered, homogenized, double pasteurized and stored. Different processing methods influenced the nutritional composition of local and CO 6 variety peanut milk. The nutritional composition like total solids, protein, fat, calcium and iron content of the different processed peanut milk were analyzed. The total solid content of the peanut milk does not change in soaking (13.98 and 15 g/ 100 mL), germination method (13.99 and 15.26 g/100 mL), less reduction in roasting method (13.96 and 15.20 g/ 100 mL), more significant reduction in blanching method (12.36 and 13.97 g/ 100 mL) in local and CO 6 variety of peanut milk respectively were recorded. Protein content was increased in germination ((6.1 and 6.4 g/ 100 mL), soaking (6.0 and 6.3 g / 100 mL), roasting method (5.6 and 5.8 g / 100 mL) and reduction in blanching method (4.1 and 5.0 g / 100 mL) in local and CO 6 variety peanut milk respectively. Fat content was significantly reduced in control (3.82 and 3.54 g / 100 mL), blanching method (3.63 and 3.32 g / 100 mL), soaking method ( 3.80 and 3.55 g/ 100 mL) and germination method (3.60 and 3.50 g/ 100 mL) in local and CO 6 variety peanut milk. Calcium and iron content was greatly reduced in blanching and lesser reduction in roasting method. Antinutritional factors like phytic acid, tannin, trypsin inhibitor, oxalate and phenol was greatly reduced in roasting and germination methods. It was observed that the nutritional properties were highly conserved and the antinutritional factor was greatly reduced in the roasting method.

Production of Peanut Milk and Its Functional, Physiochemical, Nutritional and Sensory Characteristics

This study was aimed to investigate the functional properties of peanut seeds, and physicochemical, nutritional and sensory analysis of processed peanut milk. Standard methods were used in this research to determine the functional properties and proximate composition, while minerals were determined by a flame photometer and colorimeter. Firstly, the functional proprieties of the whole and defatted kernel of peanut seeds were studied, and the best results were found in the defatted sample. Peanut milk samples were divided into four samples depends on the percentage of powder milk addition (3, 6, 9 and 12%) as well as the control sample without addition. The pH, total soluble solids, viscosity, and density of samples were (6.2-6.5, 6-16%, 3-8 mPa/s, and 1.01-1.05 g/cm3, respectively), increased with the increasing of milk powder. About the proximate analysis, the highest values of ash, protein, fat, total carbohydrates (0.8, 6.3, 6.3, and 7.5%, respectively) were found in the sample of 12% powder milk. Sodium, potassium, and calcium in the samples (3, 6, 9 and 12%) were found in the range 38-58, 55-89, and 10-18 mg/100 g, respectively. Sensory evaluation results revealed great acceptance for peanut milk made with 3% powder milk addition, while we're not found a significant difference in the color and taste of all samples and control. This study proved the possibility of using Sudanese peanut as a milk substitute which was found to be a good source of nutrients. This study suggested using peanut milk as a milk substitute, and in yogurt manufacture, future work should aim at studying optimum storage conditions and stable packaging requirements.

Effect of Storage on Physio-Chemical Properties of Peanut Yogurt

Peanuts may be consumed in a variety of processed forms like roasted, raw and processed etc. andrepresent as a multimillion dollar crop worldwide with many potential dietary benefits as it contains highprotein and health effective oils. Objective: The present investigation was planned to evaluate thephysio-chemical properties of peanut milk yogurt by the addition of different concentration of peanut milk(0 %, 10 %, 20 % and 30 %), skimmed milk liquid (60 %, 70 %, 80 %, and 90 %), skimmed milk powder (9 %)and sugar (1 %). Methods: The physio-chemical tests (pH, acidity, moisture, ash, fat, protein, syneresis,and viscosity) were examined after every 5 days of interval for a period of 15 days at 4 ºC. Results: Theresults of physio-chemical analysis revealed that pH, ash, fat, protein and viscosity decrease duringstorage period where as acidity, moisture and rate of syneresis increased during storage. Treatment T1(10 % peanut milk) was comparatively best for manufacturing of peanut milk yogurt followed by T2 (20 %peanut milk + 70 % skimmed milk liquid + 9 % skimmed milk powder + 1 % sugar) while peanut milk yogurtfrom (30 % peanut milk + 60 % skimmed milk liquid + 9 % skimmed milk powder + 1 % sugar) had the lowestdegree of firmness. Conclusions: It was noticed that correlation among fat, total solids and proteincontents in peanut milk affect the extent of serum separation and pH of yogurt. The storage hadsignificant effects on all physio-chemical parameters. Treatments had significant effect on all physiochemicalparameters.

Study on Standardisation and Quality Evaluation of Peanut Milk by Different Processing Methods

Peanut (Araches hypogea) is an important oilseed crop originated from South Africa, while India representing one of its leading producer nearly 14% of world peanut production. Now a days some of the lifestyle changes and medical issues like cow’s milk allergy, lactose intolerance and hypercholesterolemia people were shifted into plant based nondairy beverages. Peanut milk was developed from two different peanut varieties viz., TNAU CO-6 and local variety. Proximate composition of local and TNAU CO 6 variety of peanut was analyzed and the carbohydrates, protein, fat was high in TNAU CO 6 peanut variety and its value was 26.7 (g/100g), 27.8 (g/100g), 38(g/100g) when compared to local variety, its value was 25.2 (g/100g), 24.7 (g/100g), 39(g/100g) respectively. Peanut milk was extracted by five different processing method: fresh soaking, blanching, roasting and germination methods in both local and TNAU CO 6 variety. Peanut milk prepared without any treatments and processing was considered as a control peanut milk. Among the different treatment, the best treatment was selected based on sensory scores in each processing methods of both peanut varieties. Among these different treatments blanching method B1 (2mins) was best in local variety, B2(3 mins) was best in CO 6 variety. In roasting method R2 (roasting for 5 mins and soaking for 3 hrs), soaking method S2(3 hrs soaking), germination method G1(8 hrs germinated) was best treatment in both the selected peanut varieties. Based on the observation and sensory evaluation, the result showed that among these five processing methods roasting method was the best method for the peanut milk extraction and its physiochemical properties were analyzed in both the varieties of prepared peanut milk among these CO 6 variety peanut milk had good result and better acceptability.

Pengaruh Varietas dan Lama Fermentasi Terhadap Kualitas Kefir Susu Kacang Tanah (Arachis Hypogaea)

Kefir is one fermented product that has a taste, a yogurt-like consistency color and has a distinctive yeast aroma. Peanuts are economically ranked second after soybeans. As peanut food has important nutritional benefits in the human nutriency of high protein, minerals and essential fatty acids such as linoleic and oleic acids. Peanut milk contains a very high amino acid almost equivalent to the protein content of animal milk and the price is relatively cheap This study aims to determine the effect of varieties and length of fermentation on the quality of peanut milk kefir. The research design used was Completely Randomized Design (RAL) consisting of 2 factor V peanut varieties consisting of 2 levels (Hypoma 1 and Singa) and long L fermentation factor consisting of 3 levels (21, 24 and 27 hours), each of which was repeated 3 times. The observed observations were pH, total acid, alcohol content, protein content, fat content, Total Plate Count (TPC), Texture Profile Analysis (TPA). Treatment of varieties and duration of fermentation of peanut milk kefir have significant effect on pH value, total acid, fat content, Total Plate Count (TPC), significantly affect alcohol content and no significant effect on protein content, Texture Profile Analysis (TPA), organoleptic aroma, taste and texture. Quality of peanut milk kefir produced on lion varieties with 24 hours fermentation time of total acid 6.43%, alcohol content 2.84%, protein content 2.80%, Total Plate Count (TPC) 30.67 x 103 CFU and Adhesiveness 1.324 g/s.

Determination of Volatile Compounds in Nut-Based Milk Alternative Beverages by HS-SPME Prior to GC-MS Analysis.

A reliable Headspace-Solid Phase Microextraction (HS-SPME) method was developed for the determination of polar volatile components of commercial nut-based milk alternative drinks prior to Gas Chromatography–Mass Spectrometry (GC-MS) analysis. Under the optimum extraction conditions, a divinylbenzene (DVB)/Carboxen™ CAR)/polydimethylsiloxane (PDMS) fiber was used and 2 mL of sample was heated at 60 °C for 40 min under stirring, without salt addition. Ten compounds from different chemical classes (heptane, a-pinene, toluene, 2-methylpyrazine, 3-heptanone, heptanal, 2-octanone, 1-heptanol, benzaldehyde and 1-octanol) were chosen as model analytes for quantification. Limits of detection and limits of quantification were found to be 0.33–1.67 ng g−1 and 1–5 ng g−1, accordingly. Good linearity, precision and accuracy were obtained as well as a wide linear range. The proposed method was successfully applied to various beverages including almond milk, walnut milk, peanut milk and almond chocolate milk. More than 70 volatile compounds were detected in the different samples. Most of the detected volatiles were aldehydes, ketones and alcohols. This technique can be used for the determination of volatile compounds in nut-based beverages, to detect compositional changes during storage and technological treatment used for their production.

The Effect of Varying Starter Culture Content on the Characteristics of Peanut Yogurt Produced from Peanut Milk

Presently, a considerable number of people go for plant milk substitutes due to various reasons such as medical reasons or lifestyle choices. Medical reasons include lactose intolerance, cow's milk allergy and cholesterol issues.Life style choices such as vegetarian orvegandiet concerns about growth hormones or antibiotic residues in cow's milk also influence these choices. The possibility of producing fermentedpeanutbever age (Peanut yoghurt) from lactose free milk-like beverage (peanut milk) was studied. Plant milk substitutes (such as peanut milk) are suspensions of dissolved and disintegrated plant materials in water resembling cow's milk. Peanut yogurt with varying amount of starter culture was successfully produced by fermenting peanut milk. The increase in starter culture content was done in an attempt to reduce the nutty flavour which is an undesirable characteristic in the peanut milk product. The peanut yogurt improved gradually as the starter culture content increased. The peanut yogurt was subjected to physicochemical, proximate, micro biological analysis and sensory evaluation. The peanut yogurt with the highest starter culture content was discovered to have the highest viscosity value and better aroma than the other variations, it also had low fat content and high protein and carbohydrate content. Sensory evaluation of peanut yoghurt using the nine-point hedonic scale with the help of fifteen panelists showed that as the starter culture increased peanut yogurt with acceptable quality attributes was achieved. Therefore, peanut yogurt can be a successful alternative to milk yoghurt manufacture in regions with high peanut production.

The impact of roasting, high pressure homogenization and sterilization on peanut milk and its oil bodies

The effect of roasting, high-pressure homogenization HPH and thermal treatment on peanut milk and its oil bodies OBs was evaluated. Full-fat peanut milk samples were obtained using aqueous extraction method followed by HPH 150 or 300 MPs and pasteurization or sterilization. Roasting has a pronounced effect on the appearance, functional properties and the OBs protein membrane of peanut milk. The HPH significantly affected the microstructure, particle size, rheology and the color of raw and roasted peanut milk. Where, the size was significantly reduced, consistency index (k) decreased and the flow behavior index (n) increased with the increase in homogenization pressure. However, the raw peanut milk samples became more sensitive to sterilization. On the other hand, the roasted peanut milk samples subjected to homogenization were particularly stable to sterilization. Further, a combination of roasting, HPH and thermal treatment can provide a stable product with better flavor and without additives.

Culture-free, highly sensitive, quantitative detection of bacteria from minimally processed samples using fluorescence imaging by smartphone

A critical unmet need in the diagnosis of bacterial infections, which remain a major cause of human morbidity and mortality, is the detection of scarce bacterial pathogens in a variety of samples in a rapid and quantitative manner. Herein, we demonstrate smartphone-based detection of Staphylococcus aureus in a culture-free, rapid, quantitative manner from minimally processed liquid samples using aptamer-functionalized fluorescent magnetic nanoparticles. The tagged S. aureus cells were magnetically captured in a detection cassette, and then fluorescence was imaged using a smartphone camera with a light-emitting diode as the excitation source. Our results showed quantitative detection capability with a minimum detectable concentration as low as 10 cfu/ml by counting individual bacteria cells, efficiently capturing S. aureus cells directly from a peanut milk sample within 10 min. When the selectivity of detection was investigated using samples spiked with other pathogenic bacteria, no significant non-specific detection occurred. Furthermore, strains of S. aureus from various origins showed comparable results, ensuring that the approach can be widely adopted. Therefore, the quantitative fluorescence imaging platform on a smartphone could allow on-site detection of bacteria, providing great potential assistance during major infectious disease outbreaks in remote and resource-limited settings.

Synthesis of gold-palladium nanowaxberries/dodecylamine-functionalized graphene quantum dots-graphene micro-aerogel for voltammetric determination of peanut allergen Ara h 1

The paper reports synthesis of gold-palladium nanowaxberries(AuPd NWs)/dodecylamine-functionalized graphene quantum dots(D-GQDs)-graphene micro-aerogel(GMA). D-GQDs was used as a solid particle surfactant for stabilizing Pickering emulsion of toluene-in-graphene oxide aqueous dispersion. Graphene oxide sheets in the aqueous phase are reduced by hydrazine hydrate, diffused into the toluene droplet and self-assembled into graphene oxide micro-gels. Followed by freeze-drying, thermal annealing and hybridized with AuPd NWs. The as-prepared AuPd NWs/D-GQDs-GMA shows an unique three-dimensional structure with the size of microns. The small size and strong polarity make it can be directly dispersed in ethanol to form stable dispersion for sensor preparation. The hybrid of GMA, D-GQDs and AuPd NWs greatly improves the electron transfer, electroactive surface area and ion diffusion. The architecture of conductor/semiconductor/conductor achieves to a significant amplification of detection signal. The DNA biosensor based on the AuPd NWs/D-GQDs-GMA exhibits ultrasensitive differential pulse voltammetric (DPV) response towards peanut allergen Ara h 1. The DPV signal linearly increases with increasing DNA concentration in the range of 1.0 × 10−22-1.0 × 10−17 M with the detection limit of 4.7 × 10−23 M (S/N = 3). The analytical method was successfully applied to voltammetric determination of peanut allergen Ara h 1 in peanut milk beverage.