Pavlovian Research Articles

Detection of Hbv Dna By Direct Pcr Protocol And Nested Pcr Protocol And Comparing Them. In Chronic Patients and Healthy Carriers of Hepatitis B Virus.

Hepatitis B virus is a major public health problem, and the outcome of infection depends on the effectiveness of the virus's interaction with the host, and in particular on the strength of the natural and conditioned response of humoral and cellular immunity. This study was conducted to assess the clinical status, genetic immunity and viral (viral) traits of Iraqi patients with chronic hepatitis B virus and healthy carriers of hepatitis B surface antigen, as well as to show the relationship between the immune status and the chronic condition of the disease. The study included (60) patients with chronic hepatitis B virus type (B) and (60) healthy hepatitis B surface antigen carriers who attended the Teaching Hospital for Gastrointestinal and Liver Diseases in Nasiriya City Al-Hussein Teaching Hospital, Thalassemia Center and the Central Blood Bank in Nasiriya Citysouthern of Iraq. for the period from the first period. From February 2022 until the end of February 2022, the average age of patients infected with chronic hepatitis B virus was 64.5 years, and the average age of healthy carriers of hepatitis B surface antigen was 35. 1 year, and the ratio of males to females was (12.2:) for chronic patients and (13.6:) for carriers of the disease. As for the deoxyribonucleic acid, it was (100%) in chronic patients and healthy carriers using the Nested type polymerase chain reaction, and at a rate of (30.0%) and (46.7%) in chronic patients and healthy carriers, respectively using the polymerase chain reaction technique. direct with a significant difference between the two interactions. The current results confirm that a nested PCR protocol to detect HBV DNA is a more sensitive way of detecting HBV in Iraq CHB patients and carriers. Recent advanced techniques such as nucleic acid assays, quantitative assays for viral load and RT-PCR for Iraqi viral hepatitis laboratories are recommended.

Asymptotic expansion for convection-dominated transport in a thin graph-like junction

We consider for a small parameter [Formula: see text] a parabolic convection–diffusion problem with Péclet number of order [Formula: see text] in a three-dimensional graph-like junction consisting of thin curvilinear cylinders with radii of order [Formula: see text] connected through a domain (node) of diameter [Formula: see text] Inhomogeneous Robin type boundary conditions, that involve convective and diffusive fluxes, are prescribed both on the lateral surfaces of the thin cylinders and the boundary of the node. The asymptotic behavior of the solution is studied as [Formula: see text] i.e. when the diffusion coefficients are eliminated and the thin junction is shrunk into a three-part graph connected in a single vertex. A rigorous procedure for the construction of the complete asymptotic expansion of the solution is developed and the corresponding energy and uniform pointwise estimates are proved. Depending on the directions of the limit convective fluxes, the corresponding limit problems [Formula: see text] are derived in the form of first-order hyperbolic differential equations on the one-dimensional branches with novel gluing conditions at the vertex. These generalize the classical Kirchhoff transmission conditions and might require the solution of a three-dimensional cell-like problem associated with the vertex to account for the local geometric inhomogeneity of the node and the physical processes in the node. The asymptotic ansatz consists of three parts, namely, the regular part, node-layer part, and boundary-layer one. Their coefficients are classical solutions to mixed-dimensional limit problems. The existence and other properties of those solutions are analyzed.

CK2 negatively regulates the extinction of remote fear memory

Cognitive behavioral therapy, rooted in exposure therapy, is currently the primary approach employed in the treatment of anxiety-related conditions, including post-traumatic stress disorder (PTSD). In laboratory settings, fear extinction in animals is a commonly employed technique to investigate exposure therapy; however, the precise mechanisms underlying fear extinction remain elusive. Casein kinase 2 (CK2), which regulates neuroplasticity via phosphorylation of its substrates, has a significant influence in various neurological disorders, such as Alzheimer’s disease and Parkinson’s disease, as well as in the process of learning and memory. In this study, we adopted a classical Pavlovian fear conditioning model to investigate the involvement of CK2 in remote fear memory extinction and its underlying mechanisms. The results indicated that the activity of CK2 in the medial prefrontal cortex (mPFC) of mice was significantly upregulated after extinction training of remote cued fear memory. Notably, administration of the CK2 inhibitor CX-4945 prior to extinction training facilitated the extinction of remote fear memory. In addition, CX-4945 significantly upregulated the expression of p-ERK1/2 and p-CREB in the mPFC. Our results suggest that CK2 negatively regulates remote fear memory extinction, at least in part, by inhibiting the ERK-CREB pathway. These findings contribute to our understanding of the underlying mechanisms of remote cued fear extinction, thereby offering a theoretical foundation and identifying potential targets for the intervention and treatment of PTSD.

Ionic Diffusive Nanomemristors with Dendritic Competition and Cooperation Functions for Ultralow Voltage Neuromorphic Computing.

Realization of dendric signal processing in the human brain is of great significance for spatiotemporal neuromorphic engineering. Here, we proposed an ionic dendrite device with multichannel communication, which could realize synaptic behaviors even under an ultralow action potential of 80 mV. The device not only could simulate one-to-one information transfer of axons but also achieve a many-to-one modulation mode of dendrites. By the adjustment of two presynapses, Pavlov's dog conditioning experiment was learned successfully. Furthermore, the device also could emulate the biological synaptic competition and synaptic cooperation phenomenon through the comodulation of three presynapses, which are crucial for artificial neural network (ANN) implementation. Finally, an ANN was further constructed to realize highly efficient and anti-interference recognition of fashion patterns. By introducing the cooperative device, synaptic weight updates could be improved for higher linearity and larger dynamic regulation range in neuromorphic computing, resulting in higher recognition accuracy and efficiency. Such an artificial dendric device has great application prospects in the processing of more complex information and the construction of an ANN system with more functions.

Impact of enriched environment on motor performance and learning in mice

Neuroscience heavily relies on animal welfare in laboratory rodents as it can significantly affect brain development, cognitive function and memory formation. Unfortunately, laboratory animals are often raised in artificial environments devoid of physical and social stimuli, potentially leading to biased outcomes in behavioural assays. To assess this effect, we examined the impact of social and physical cage enrichment on various forms of motor coordination. Our findings indicate that while enriched-housed animals did not exhibit faster learning in eyeblink conditioning, the peak timing of their conditioned responses was slightly, but significantly, improved. Additionally, enriched-housed animals outperformed animals that were housed in standard conditions in the accelerating rotarod and ErasmusLadder test. In contrast, we found no significant effect of enrichment on the balance beam and grip strength test. Overall, our data suggest that an enriched environment can improve motor performance and motor learning under challenging and/or novel circumstances, possibly reflecting an altered state of anxiety.

Choosing What You Like or Liking What You Chose? Sampling’s Impact on Evaluation and the Role of Idiosyncratic Reactions to Valent Stimuli

Recent work incorporating autonomy into an evaluative conditioning procedure provided evidence of a sampling decision effect in which high-autonomy participants positively shifted their evaluations of frequently sampled conditioned stimuli (CSs), regardless of whether they were consistently paired with positive or negative unconditioned stimuli (USs). The current work modified this paradigm by also measuring participants’ evaluations of the sampled USs. Two experiments replicate the sampling decision effect for neutral CSs in a new variant of the paradigm while ruling out the alternative possibility that the effect is driven by idiosyncratic variance in participants’ reactions to the USs. In addition, Experiment 2 suggests that the sampling decision effect does not extend to the paired, valent stimuli. Together, these results further suggest that it is the act of sampling a stimulus more frequently that predicts a positive evaluative shift toward it.

Free Vibration of Annular Plates Constrained by Translational and/or Rotational Springs on Outer and Inner Edges

This paper presents comprehensive lists of natural frequencies for vibration of thin isotropic annular plates with outer and inner edges elastically constrained by translational and rotational springs. A method is extended from a previously presented Ritz approach to include effects of the springs. In numerical examples, sixteen models are considered to cover all general cases of locating translational and/or rotational springs on outer and/or inner edges in addition to classical boundary conditions of free and simple supported edges. After convergence study and comparison test in specific cases are made to validate solution accuracy, frequency parameters of the sixteen models are summarized and frequency variations with increasing spring stiffness are illustrated.

An Analytical Solution for the Bending of Anisotropic Rectangular Thin Plates with Elastic Rotation Supports

The mechanical analysis of thin-plate structures is a major challenge in the field of structural engineering, especially when they have nonclassical boundary conditions, such as those encountered in cement concrete road slabs connected by transfer bars. Conventional analytical solutions are usually limited to classical boundary conditions—clamped support, simple support, and free edges—and cannot adequately describe many engineering scenarios. In this study, an analytical solution to the bending problem of an anisotropic thin plate subjected to a pair of edges with free opposing elastic rotational constraints is found using a two-dimensional augmented Fourier series solution method. In the derivation process, the thin-plate problem can be transformed into a problem of solving a system of linear algebraic equations by applying Stoke’s transform method, which greatly reduces the mathematical difficulty of solving the problem. Complex boundary conditions can be optimally handled without the need for large computational resources. The paper addresses the exact analytical solutions for bending problems with multiple combinations of boundary conditions, such as contralateral free–contralateral simple support (SFSF), contralateral free–contralateral solid support–simple support (CFSF), and contralateral free–contralateral clamped support (CFCF). These solutions are realized by employing the Stoke transformation and adjusting the spring parameters in the analyzed solutions. The results of this method are also compared with the finite element method and analytical solutions from the literature, and good agreement is obtained, demonstrating the effectiveness of the method. The significance of the study findings lies in the simplification of complex nonclassical boundary condition problems using a simple and reliable analytical method applicable to a wide range of engineering thin-plate structures.

Counterirritation by pain inhibits the responsiveness to aversive loud tones: the role of state anxiety and state fear triggered in the NPU paradigm

Aim of the study The application of a noxious stimulus reduces the perception and responsiveness to other pain stimuli. This inhibition can be experimentally assessed with a method called ‘counterirritation’. The question arises if counterirritation acts also on the perception and responsiveness to aversive but non-nociceptive stimuli (e.g., loud tones). Since aversive stimulation is often associated with state anxiety or state fear, we investigated in addition the modulatory effects of these emotions on counterirritation. Material and Methods 51 subjects participated in our study. We presented tones with aversive loudness (105 dB), first alone then during counterirritation (immersion of the hand in a hot water bath of 46 °C) to assess inhibition of loudness perception and responsiveness. Influences of state anxiety and state fear on counterirritation were investigated by using the Neutral-Predictable(fear)- Unpredictable(anxiety) Paradigm (NPU), which is based on classical conditioning. Loudness ratings (perception of the aversive tones) and startle reflex (defensive reaction to aversive tones) were assessed. Results Counterirritation reduced startle reflex amplitudes, but not the loudness ratings. Although state anxiety and state fear were successfully induced, counterirritation remained unaffected. Conclusions Our study showed that pain inhibits the responsiveness to aversive stimuli (loud tones). Thus, the postulate that ‘pain inhibits pain’ might be better changed to ‘pain inhibits aversiveness’. Consequently, our findings may also question the assumption of a clear pain specificity in inhibitory action as assumed by theoretical approaches like ‘conditioned pain modulation’ (CPM). Furthermore, counterirritation appeared one more time resistant to the influence of negative emotions.

Reactivation of encoding ensembles in the prelimbic cortex supports temporal associations

Fear conditioning is encoded by strengthening synaptic connections between the neurons activated by a conditioned stimulus (CS) and those activated by an unconditioned stimulus (US), forming a memory engram, which is reactivated during memory retrieval. In temporal associations, activity within the prelimbic cortex (PL) plays a role in sustaining a short-term, transient memory of the CS, which is associated with the US after a temporal gap. However, it is unknown whether the PL has only a temporary role, transiently representing the CS, or is part of the neuronal ensembles that support the retrieval, i.e., whether PL neurons support both transient, short-term memories and stable, long-term memories. We investigated neuronal ensembles underlying temporal associations using fear conditioning with a 5-s interval between the CS and US (CFC-5s). Controls were trained in contextual fear conditioning (CFC), in which the CS-US overlaps. We used Robust Activity Marking (RAM) to selectively manipulate PL neurons activated by CFC-5s learning and Targeted Recombination in Active Populations (TRAP2) mice to label neurons activated by CFC-5s learning and reactivated by memory retrieval in the amygdala, medial prefrontal cortex, hippocampus, perirhinal cortices (PER) and subiculum. We also computed their co-reactivation to generate correlation-based networks. The optogenetic reactivation or silencing of PL encoding ensembles either promoted or impaired the retrieval of CFC-5s but not CFC. CFC-5s retrieval reactivated encoding ensembles in the PL, PER, and basolateral amygdala. The engram network of CFC-5s had higher amygdala and PER centralities and interconnectivity. The same PL neurons support learning and stable associative memories.

Social buffering in rats reduces fear by oxytocin triggering sustained changes in central amygdala neuronal activity

The presence of a companion can reduce fear, but the neural mechanisms underlying this social buffering of fear are incompletely known. We studied social buffering of fear in male and female, and its encoding in the amygdala of male, auditory fear-conditioned rats. Pharmacological, opto,- and/or chemogenetic interventions showed that oxytocin signaling from hypothalamus-to-central amygdala projections underlied fear reduction acutely with a companion and social buffering retention 24 h later without a companion. Single-unit recordings with optetrodes in the central amygdala revealed fear-encoding neurons (showing increased conditioned stimulus-responses after fear conditioning) inhibited by social buffering and blue light-stimulated oxytocinergic hypothalamic projections. Other central amygdala neurons showed baseline activity enhanced by blue light and companion exposure, with increased conditioned stimulus responses that persisted without the companion. Social buffering of fear thus switches the conditioned stimulus from encoding “fear” to “safety” by oxytocin-mediated recruitment of a distinct group of central amygdala “buffer neurons”.

Acute MK-801 increases measures of both sign-tracking and goal-tracking in male Sprague-Dawley rats

Sign-tracking is a Pavlovian conditioned approach behavior thought to be important in understanding cue-driven relapse to drug use, and strategies for reducing sign-tracking may have some benefit in preventing relapse. A previous study successfully employed the NMDA receptor antagonist MK-801 in preventing the development of sign-tracking (but not goal-tracking) in a conditioned approach task. In this study, we focused on whether MK-801 would have similar effects on previously established sign-tracking behavior. MK-801 was administered after training in a standard sign−/goal-tracking task using a retractable lever as a conditioned stimulus and a sucrose pellet as unconditioned stimulus. It was found that MK-801 increased measures of both sign- and goal-tracking in subjects who had previously learned the task. The NMDA receptor appears to play a complex role in governing behavior related to sign-tracking.

The effects of a retrieval cue on renewal of conditioned responses in human appetitive conditioning

Contextual renewal of reward anticipation may be one potential mechanism underlying relapse in eating and substance use disorders. We therefore tested retrieval cues, a method derived from an inhibitory retrieval-based model of extinction learning to attenuate contextual renewal using an appetitive conditioning paradigm. A pilot study was carried out in Experiment 1 to validate a differential chocolate conditioning paradigm, in which a specific tray was set up as a conditioned stimulus (CS) for eating chocolate (unconditioned stimulus, US). Using an ABA renewal design in Experiment 2, half of the participants were presented with a retrieval cue in the acquisition phase (group AC) and the other half in the extinction phase (group EC). Presentation of the retrieval cue in the EC was associated with reduced renewal of US-expectancy, while there was a clear renewal effect for US-expectancy in the AC. One limitation was the difference in cue presentations between both groups due to the number of trials in acquisition and extinction. Experiment 3 therefore aimed at replicating the results of Experiment 2, but with fewer cue presentations for the EC to match the AC. No significant group differences were observed indicating no effect of the retrieval cue. Theoretical and clinical implications in light of the differing results are discussed.

Associative Visuomotor Learning Using Transcranial Magnetic Stimulation Induces Stimulus-Response Interference.

Classical conditioning states that the systematic co-occurrence of a neutral stimulus with an unconditioned stimulus can cause the neutral stimulus to, over time, evoke the same response as the unconditioned stimulus. On a neural level, Hebbian learning suggests that this type of learning occurs through changes in synaptic plasticity when two neurons are simultaneously active, resulting in increased connectivity between them. Inspired by associative learning theories, we here investigated whether the mere co-activation of visual stimuli and stimulation of the primary motor cortex using TMS would result in stimulus-response associations that can impact future behavior. During a learning phase, we repeatedly paired the presentation of a specific color (but not other colors) with a TMS pulse over the motor cortex. Next, participants performed a two-alternative forced-choice task where they had to categorize simple shapes and we studied whether the shapes' task-irrelevant color (and its potentially associated involuntary motor activity) affected the required motor response. Participants showed more errors on incongruent trials for stimuli that were previously paired with high intensity TMS pulses, but only when tested on the same day. Using a drift diffusion model for conflict tasks, we further demonstrate that this interference occurred early, and gradually increased as a function of associated TMS intensity. Taken together, our findings show that the human brain can learn stimulus-response associations using externally induced motor cortex stimulation. Although we were inspired by the Hebbian learning literature, future studies should investigate whether Hebbian or other learning processes were also what brought about this effect.

Mouse Model of Chronic Social Stress-Induced Excessive Pavlovian Aversion Learning-Memory.

Increased experience of aversive stimuli/events is a psychological-neurobiological state of major importance in psychiatry. It occurs commonly in generalized anxiety disorder, post-traumatic stress disorder, and major depression. A sustained period of exposure to threat (chronic stressor) is a common risk factor, and a major symptom is generalized excessive perception of, and reactivity to, aversive stimuli. In rodents, Pavlovian aversion learning and memory (PAL, PAM), quantified in terms of the conditioned defensive behavior freezing, is an extensively studied behavioral paradigm, and well understood in terms of underlying neural circuitry. In mice, chronic social stress (CSS) is a 15-day resident-intruder paradigm in which C57BL/6 adult males are exposed continuously and distally to dominant-aggressive CD-1 male mice (sustained threat) interspersed with a brief daily period of proximal attack (acute threat). To ensure that physical wounding is minimized, proximal attacks are limited to 30 to 60 s/day and lower incisor teeth of CD-1 mice are blunted. Control (comparison) mice are maintained in littermate pairs. The CSS and CD-1 mice are maintained in distal contact during subsequent behavioral testing. For PAL, CSS and control (CON) mice are placed in a conditioning chamber (context) and exposed to a tone [conditioned stimulus (CS)] and mild, brief foot shock [unconditioned stimulus (US)]. For PAM, mice are placed in the same context and presented with CS repetitions. The CSS mice acquire (learn) and express (memory) a higher level of freezing than CON mice, indicating that CSS leads to generalized hypersensitivity to aversion, i.e., chronic social aversion leads to increased aversion salience of foot shock. Distinctive features of the model include the following: high reproducibility; rare, mild wounding only; male specificity; absence of "susceptible" vs "resilient" subgroups; behavioral effects dependent on continued presence of CD-1 mice; and preclinical validation of novel compounds for normalizing aversion hypersensitivity with accurate feedforward prediction of efficacy in human patients. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Chronic social stress (CSS) Basic Protocol 2: Pavlovian aversion learning and memory (PALM).

Contingency Reversal in Conditioned Fear Learning: The Moderated Mediation Model of Intolerance of Uncertainty and Instruction.

The study aimed to examine the roles of anxiety and intolerance of uncertainty (IU) in conditioned fear learning under an uncertain context induced by the contingency reversal of the association between the conditioned stimulus and the unconditioned stimulus (CS-US). The study sample comprised 53 participants, randomly divided into two groups: a non-instruction group and an instruction group. The experimental procedure encompassed five stages: pre-acquisition, acquisition, generalization, reversal acquisition, and reversal generalization. Our study primarily focused on analyzing a moderated mediation model. In the instructed group, we observed that the reversed fear generalization response was directly influenced by the pre-reversal fear generalization response, while also being indirectly mediated by the IU factor. However, in the non-instructed group, we did not find a significant mediating effect of IU. Moreover, we noted that the mediation of IU was contingent on the instructional information. It is noteworthy that anxiety did not exhibit a discernible role in conditioned fear within the uncertainty condition in our study. The findings provide novel insights into fear-related phenomena, emphasizing the intricate interplay between individual traits and fear generalization under conditions of uncertainty. They contribute to understanding the mechanisms of emotional and cognitive interactions in uncertain conditions.

HIV Interacts with Posttraumatic Stress Disorder to Impact Fear Psychophysiology in Trauma-Exposed Black Women.

The prevalence of posttraumatic stress disorder (PTSD) among people living with HIV (PLWH) is higher than in the general population and can impact health behaviors. The influence of HIV on PTSD psychophysiology requires further investigation due to implications for the treatment of PTSD in PLWH. Utilizing fear-potentiated startle (FPS), we aimed to interrogate the influence of PTSD and HIV on fear responses. Women (18-65 years of age) recruited from the Women's Interagency HIV Study in Atlanta, GA (n = 70, 26 without HIV and 44 with HIV), provided informed consent and completed a semistructured interview to assess trauma exposure and PTSD symptom severity. Participants also underwent an FPS paradigm to assess fear acquisition and extinction: Psychophysiological indices that measure how individuals learn new fear and then subsequently attempt to suppress this fear. Women with PTSD, who did not have HIV, exhibited a greater startle response compared to women without PTSD or HIV during late acquisition to both the danger cue, reinforced conditioned stimulus (CS+, p = 0.013)), and the safety cue, non-reinforced conditioned stimulus (CS-, p = 0.046)), whereas women living with HIV (WLH) and PTSD demonstrated blunted fear responses compared to women with PTSD only. During extinction, WLH comorbid with PTSD exhibited an increased fear response during the extinction period in comparison to all other groups (p = 0.023). Women without PTSD demonstrated a reduction in the fear response during extinction regardless of HIV status. Our findings indicate that HIV further modifies fear psychophysiology in WLH with comorbid PTSD, highlighting the importance of considering HIV status in conjunction with PTSD treatment.

Learning and Neural Activity: Beyond Localization

Learning is classified into two types: "classical conditioning," which modifies simple reflexes, and "operant conditioning," which modifies complex voluntary behaviors. The neural circuits underlying these two types differ significantly. During the learning process of operant conditioning tasks, various changes in firing rate and firing synchrony of neurons can be observed across multiple brain regions. Additionally, neuronal firing rate and synchrony in several brain regions can be voluntarily controlled through operant conditioning. Consequently, it is evident that neurons in widespread brain regions have the potential for plastic changes to facilitate learning. It may be suggested that the learning of complex voluntary behaviors is underlined by widespread dynamic changes in neural activity and is not restricted to only a few brain regions.

On the Hinch–Kim dualism between singularity and Faxén operators in the hydromechanics of arbitrary bodies in Stokes flows

We generalize the multipole expansion and the structure of the Faxén operator in Stokes flows obtained for bodies with no-slip to generic boundary conditions, addressing the assumptions under which this generalization is conceivable. We show that a disturbance field generated by a body immersed in an ambient flow can be expressed, independently on the boundary conditions, as a multipole expansion, the coefficients of which are the moments of the volume forces. We find that the dualism between the operator giving the disturbance field of an nth order ambient flow and the nth order Faxén operator, referred to as the Hinch–Kim dualism, holds only if the boundary conditions satisfy a property that we call Boundary-Condition reciprocity (BC-reciprocity). If this property is fulfilled, the Faxén operators can be expressed in terms of the (m, n)th order geometrical moments of the volume forces (defined in the article). In addition, it is shown that in these cases, the hydromechanics of the fluid-body system is completely determined by the entire set of the Faxén operators. Finally, classical boundary conditions of hydrodynamic applications are investigated in light of this property: boundary conditions for rigid bodies, Newtonian drops at the mechanical equilibrium, porous bodies modeled by the Brinkman equations are BC-reciprocal, while deforming linear elastic bodies, deforming Newtonian drops, non-Newtonian drops, and porous bodies modeled by the Darcy equations do not have this property. For Navier-slip boundary conditions on a rigid body, we find the analytical expression for low order Faxén operators. By using these operators, the closed form expressions for the flow past a sphere with arbitrary slip length immersed in shear and quadratic flows are obtained.

Casimir wormhole solutions in [formula omitted] gravity

In this paper, we study the Casimir effect on the wormhole geometry in f(R,Lm) gravity. We derive the field equations for the generic f(R,Lm) function by assuming static and spherically symmetric Morris–Thorne wormhole metric. Then we consider two non-linear f(R,Lm) models, specifically, f(R,Lm)=R2+Lmβ and f(R,Lm)=R2+(1+αLm)Lm where α and β are free parameters. We derive the shape functions for wormholes by utilizing the Casimir effect and examining their existence. Subsequently, we analyse the obtained wormhole solutions for each scenario, assessing the energy conditions at the wormhole throat with a radius of r0. Our findings reveal that for some arbitrary quantities, there is a violation of classical energy conditions at the wormhole throat. Additionally, we delve into the behaviour of the equation of state (EoS) for each case. Furthermore, we explore the stability of the Casimir effect wormhole solutions by employing the generalized Tolman–Oppenheimer–Volkoff (TOV) equation. Finally, we utilize the volume integral quantifier to determine the amount of exotic matter required near the wormhole throat for both models.