Patuletin Research Articles

Nanomedicine: Patuletin-conjugated with zinc oxide exhibit potent effects against Gram-negative and Gram-positive bacterial pathogens.

With the emergence of drug-resistance, there is a need for novel anti-bacterials or to enhance the efficacy of existing drugs. In this study, Patuletin (PA), a flavanoid was loaded onto Gallic acid modified Zinc oxide nanoparticles (PA-GA-ZnO), and evaluated for antibacterial properties against Gram-positive (Bacilluscereus andStreptococcus pneumoniae)and Gram-negative (Samonella entericaand Escherichia coli) bacteria. Characterization of PA, GA-ZnO and PA-GA-ZnO' nanoparticles was accomplished utilizing fourier-transform infrared spectroscopy, efficiency of drug entrapment, polydispersity index, zeta potential, size, and surface morphology analysis through atomic force microscopy. Using bactericidal assays, the results revealed that ZnO conjugation displayed remarkable effects and enhanced Patuletin's effects against both Gram-positive and Gram-negative bacteria, with the minimum inhibitory concentration observed at micromolar concentrations. Cytopathogenicity assays exhibited that the drug-nanoconjugates reduced bacterial-mediated human cell death with minimal side effects to human cells. When tested alone, drug-nanoconjugates tested in this study showed limited toxic effects against human cells in vitro. These are promising findings, but future work is needed to understand the molecular mechanisms of effects of drug-nanoconjugates against bacterial pathogens, in addition to in vivo testing to determine their translational value. This study suggests that Patuletin-loaded nano-formulation (PA-GA-ZnO) may be implicated in a multi-target mechanism that affects both Gram-positive and Gram-negative pathogen cell structures, however this needs to be ascertained in future work.

Preparation and Characterization of Patuletin-Loaded Chitosan Nanoparticles with Improved Selectivity and Safety Profiles for Anticancer Applications

Objective. This study aimed to investigate the preparation of patuletin-encapsulated chitosan nanoparticles (PT-CS-NPs) using the ionic gelation method, evaluate their potential as anticancer agents, and invent a new method of differential pulse voltammetric analysis for patuletin (PT). Methods. Computational studies were conducted to assess the affinity of PT for chitosan, confirming a promising interaction. PT-CS-NPs were synthesized based on the computational outputs, resulting in nanoparticles with an angular structure and an average size of 6.168 nm. The cytotoxicity of PT and PT-CS-NP was evaluated in breast and colon cancer cell lines, and selectivity indices were calculated to assess safety profiles. The electrochemical behavior of PT was also investigated using the Britton–Robinson buffer. Results. PT-CS-NP exhibited both potent cytotoxicity and a favorable safety profile, showing the most active and safest pattern of cytotoxicity among the tested compounds. The electrochemical oxidation of PT was observed at 0.531 V vs. Ag/AgCl at pH 4.0.0. The concentration of PT showed a linear relationship with the corresponding peak current over the range of 10.0 x 10 − 9 : 10.0 x 10 − 5 M M, with a minimum limit of detection of 3.4 × 10(−9)M. The proposed method successfully measured PT, with a relative standard deviation below 2%. Conclusion. The preparation of PT-CS-NP via the ionic gelation method resulted in angular nanoparticles with a promising anticancer activity and safety profiles. The electrochemical behavior of PT was characterized, and a reliable method for PT quantification was established.

Antihypertensive effect of patulitrin and other constituents from Tagetes patula L. (French marigold) in acute L-NAME induced hypertensive rats

The work is aimed to evaluate the blood pressure reducing effect of constituents from methanol extract and associated constituents of Tagetes patula flowers in normotensive and L-NAME induced hypertensive rats. The HPLC analysis of methanol extract of Tagetes patula flowers (JFM) resulted in the quantitative identification and percent comparison of four phenolic constituents, protocatechuic acid (PA), methyl protocatechuate (MPA), patulitrin (TRIN) and patuletin (PAT). All the extracts, fractions and compounds examined showed significant blood pressure lowering activity. Patulitrin (TRIN) which has emerged as the major constituent (15.33%) of T. patula flowers showed significant 30% and 68% fall in blood pressure in normotensive and L-NAME induced hypertensive rats respectively. The patuletin (PAT), which is an aglycone of TRIN displayed high percentage (84%) of antihypertensive activity. Further, comprehensive and advanced studies on these constituents may result in preparation of an effective blood pressure lowering medicine with active precious rare flavonoids, patuletin and patulitrin.

Flavonoids of Artemisia spicigera

23 wild Artemisia species grow in Turkey (1,2) and some of them have not yet been investigated chemically. This report is one of the series on the chemical investigations of the Turkish Artemisia species (3-8). Seven flavonoids (hispidulin, apigenin, luteolin, quercetin, apigenin 7- glucoside, patuletin 3-glucoside and mtin) have been isolated from the aerial parts of A.spicigera. Key Words: Artemisia spicigera, flavonoids.

Flavonoid glycosides of Kalanchoe spathulata

A new glycoside, patuletin 3,7-di- O-rhamnoside, together with patuletin, quercetin, quercetin 3- O-glucoside-7- O-rhamnoside, kaempferol and kaempferol 3- O-rhamnoside were identified from leaves and flowers of Kalanchoe spathulata.

Flavonoids from Brickellia chlorolepis and B. dentata

Eleven flavonoids including three new glycosides were isolated from Brickellia chlorolepis and one new and nine known flavonoids were obtained from B. dentata. The new glycosides from B. chlorolepis are 6-methoxykaempferol 3-rhamnoglucoside, spinacetin 3-rhamnogalactoside and veronicafolin 3-rhamnoglucoside. The known compounds identified from B. chlorolepis are patuletin, casticin, artemetin, eupatolitin 3-galactoside, quercetin 3-rhamnogalactoside, rutin, isorhamnetin 3-galactoside and eupatin 3-SO 3Ca 1 2 . B. dentata contains the new glycoside eupalitin 3-galactoside and nine known compounds: pectolinarigenin, salvigenin, eupafolin, cirsiliol, eupatorin, eupatolitin, eupatolitin 3-glucoside, eupatolitin 3-galactoside and eupatin.

Constitution of patuletin

Constitution of patuletin