Termination Patterns Research Articles

Relationship between functional structures and horizontal connections in macaque inferior temporal cortex

Horizontal connections in anterior inferior temporal cortex (ITC) are thought to play an important role in object recognition by integrating information across spatially separated functional columns, but their functional organization remains unclear. Using a combination of optical imaging, electrophysiological recording, and anatomical tracing, we investigated the relationship between stimulus-response maps and patterns of horizontal axon terminals in the macaque ITC. In contrast to the “like-to-like” connectivity observed in the early visual cortex, we found that horizontal axons in ITC do not preferentially connect sites with similar object selectivity. While some axon terminal patches shared responsiveness to specific visual features with the injection site, many connected to regions with different selectivity. Our results suggest that horizontal connections in anterior ITC exhibit diverse functional connectivity, potentially supporting flexible integration of visual information for advanced object recognition processes.

Functional anatomy and topographical organization of the frontotemporal arcuate fasciculus

Traditionally, the frontotemporal arcuate fasciculus (AF) is viewed as a single entity in anatomo-clinical models. However, it is unclear if distinct cortical origin and termination patterns within this bundle correspond to specific language functions. We use track-weighted dynamic functional connectivity, a hybrid imaging technique, to study the AF structure and function in two distinct datasets of healthy subjects. Here we show that the AF can be subdivided based on dynamic changes in functional connectivity at the streamline endpoints. An unsupervised parcellation algorithm reveals spatially segregated subunits, which are then functionally quantified through meta-analysis. This approach identifies three distinct clusters within the AF - ventral, middle, and dorsal frontotemporal AF - each linked to different frontal and temporal termination regions and likely involved in various language production and comprehension aspects. Our findings may have relevant implications for the understanding of the functional anatomy of the AF as well as its contribution to linguistic and non-linguistic functions.

Aglycone Analysis and Quantitative Tracking of Correlated Terminal Glycan Pair on Spermatozoa.

Mammalian sperm glycans directly mediate several key life events. However, previous studies have not focused on two key factors that regulate these processes, the terminal glycan pattern and the anchoring sites. Herein, we group the capping monosaccharide sialic acid (Sia) and its capping substrates galactose/N-acetylgalactosamine (Gal/GalNAc) into a "correlated terminal glycan pair" (glycopair) and, for the first time, reveal the differences in the aglycone pattern of this pair on spermatozoa using glyco-selective in situ covalent labeling techniques. Sia is mainly found in glycoproteins, whereas terminal Gal/GalNAc is mainly found in glycolipids. We quantitatively track the dynamic changes of the glycopair during sperm epididymal migration and find that the Sia capping ratio decreases with the increased expression of the glycopair; caudal upswim spermatozoa also show a lower Sia capping ratio than down spermatozoa. We thus propose two new parameters reflecting the terminal glycoforms of spermatozoa, which can well distinguish the maturity of spermatozoa. By fluorescence imaging of the glycopair in different regions of the sperm, we find that different parts of the sperm contribute differently to the overall glycan changes.

A prospective observational study on the anatomical variations of recurrent laryngeal nerve and its application in the prevention of injury during thyroid surgery in a tertiary care centre

BackgroundThyroid gland is the largest and most vascular structure among the endocrine glands. The recurrent laryngeal nerve and the superior laryngeal nerve are closely related to the thyroid gland; these nerves control voice and are vital in breathing. Anatomical preservation of the recurrent laryngeal nerve (RLN) is the gold standard in thyroid surgery. Visual identification of RLN has decreased the rates of permanent RLN palsy during thyroid and parathyroid operations. Prior knowledge about the branching patterns will help in predicting and preventing injury to the nerve during thyroid surgery.Aim and objectivesThe aim of the study is to observe the terminal branching pattern of recurrent laryngeal nerve before its entry into the larynx.MethodsAn observational study with 50 participants who fulfilled the inclusion and exclusion criteria were enrolled in the study. After a complete evaluation and pre-anaesthetic checkup, the patients were operated and intraoperative findings of RLN branching were recorded and compared for the study.ResultsIn our study, out of the 50 patients who underwent thyroid surgery during the course of the study, right-sided RLN branching was demonstrated in 25 patients (21 right hemithyroidectomy and 4 total thyroidectomy). Ten patients had RLN bifurcation, 7 had trifurcation, 5 had numerous branches, and 3 with no branching pattern were noted. Similarly, out of 29 patients (25 left hemithyroidectomy and 4 total thyroidectomy) in whom left-sided RLN branching was studied, bifurcation was seen in 10 patients, trifurcation in 12 patients, and multiple branching was observed in 7.ConclusionThe anatomical variations seen in RLN may increase the risk of vocal cord palsy due to its injury. Anatomical variations are categorized according to the original direction of the RLN and the number of terminal branches in the larynx. This classification aids in the identification and preservation of its branches, hence facilitating a safer thyroidectomy.

How Children Stop Adult Perpetrators From Unwanted Touching Within an Institutional Setting: Termination Patterns During Sexual Contact Stage.

This qualitative study analyzes victims' narratives of male-on-male child molestation within the Boy Scouts of America. The focus of previous literature on sex crime victims mostly centered on how to avoid sex offenders based on the offenders' behaviors and effective victims' treatment plans to alleviate their posttrauma as a result of sexual abuse. Yet studies have not provided detailed descriptions of when and how to resist sexual advances to children when such incidents are in progress within youth-oriented institutions. Few have examined victim narratives to discover the dynamics of child molestation, such as physical and verbal interactions between the two parties while sexual touching is occurring. The author used victims' narratives to identify how boy victims responded to unwanted sexual advances by scout leaders within the Boy Scouts of America. Based on qualitative-oriented analysis, this study presents verbal and physical resisting patterns of male victims and the effectiveness of such actions in terminating sexual advances. The findings suggest practical and potential intervention strategies so children know how to stop adult perpetrators from developing unwanted touching within youth-centric institutions.

Carbon dioxide emission equivalent calculation and inter-sectoral transfer pattern of different water use terminals in China

In the context of global climate change, all industries have put forward the requirements for carbon emission reduction. The effective use of water resources is the key to achieving carbon emission reduction, so it is particularly important to review the carbon dioxide emission equivalents of water resource utilization behaviors (CEE-WRUBs) of water users. However, existing research on CEE-WRUBs across various water sectors remains inadequate. Therefore, this study integrates the enhanced CEEA method, LMDI decomposition model, and IOA method to analyze CEE-WRUBs across diverse water use terminals. Then seeks their main driving factors and their transfer pattern among different industries, which are crucial to the realization of the global carbon neutrality objective. The results showed that: (1) China's CEE-WRUBs show a significant downward trend. Industrial water use emerges as the primary source of CEE, but the CEE-WRUBs of most industrial water terminals show a fluctuating downward trend. Grains' water use behavior (WRUBs) absorbed the largest CEE, reaching 14,698 Mt in 2020. (2) the water efficiency effect emerges as the predominant driver behind the increase in CEE-WRUBs most of the time, and holding a prominent position. The carbon emission intensity effect primarily steers the reduction of CEE-WRUBs. (3) the largest net outflow sector of WRUBs embodied carbon in 2007–2020 was transformed from basic material heavy industry (−153.54 Mt in 2007) to agriculture (−128.26 Mt in 2020). Most of the WRUBs embodied carbon of agriculture flows into light industry, while most of the WRUBs embodied carbon of basic material heavy industry flows into finishing heavy industry and construction. The methods and results of this study provide a potential reference for investigating the regional water-carbon relationship and advancing the global carbon neutrality objective.

Extratemporal Facial Nerve Interconnections and Trunk's Variability: A Systematic Review with Meta-Analysis.

The present systematic review with meta-analysis is a significant contribution to the understanding of the morphological variability of the facial nerve (FN) extratemporal segment, i.e., the facial trunk (FT) variability, its division, and terminal branching patterns. The study also provides a comprehensive overview of the clinical significance of the FN extracranial division. Four online databases were utilized to conduct the systematic review according to evidence-based anatomy guidelines. A meta-analysis of the studies included was carried out using R programming software. The combined prevalence of the FN variants was calculated, along with subgroup and cumulative analysis. From the systematic review, 29 studies were retrieved as eligible for our initial purpose. However, 19 studies followed the same classification system and were selected for the meta-analysis, with a total sample of 2453 nerves. The most common pattern of the FN morphology was the FT bifurcation (typical pattern), with a pooled prevalence of 94.1% and a single interconnection (IC) between the temporofacial and cervicofacial branches (23.1% pooled prevalence). Two ICs between these branches were the rarest pattern (8.9% pooled prevalence). Our findings underscore the extensive morphological variability of the FN extratemporal anatomy, which has led to confusion among researchers. While several classification systems have been developed, none accurately represent the typical and variant anatomy. Our meta-analysis provided a small range between 8.9-23.1% for the rarest and most common pattern; thus, diversity is the rule. Therefore, it is not safe to conclude the typical morphology of FN extratemporal anatomy for its whole distribution before the FT's division (proximally) and its terminal branches (distally). Nevertheless, the bifurcation of the FT can be considered the typical morphology, and it is far more constant than the distal branching pattern. These findings have significant implications for surgical procedures, particularly parotidectomy, where surgeons must exercise utmost caution due to the potential clinical implications of FN injury.

Semaphorin7A patterns neural circuitry in the lateral line of the zebrafish

In a developing nervous system, axonal arbors often undergo complex rearrangements before neural circuits attain their final innervation topology. In the lateral line sensory system of the zebrafish, developing sensory axons reorganize their terminal arborization patterns to establish precise neural microcircuits around the mechanosensory hair cells. However, a quantitative understanding of the changes in the sensory arbor morphology and the regulators behind the microcircuit assembly remain enigmatic. Here, we report that Semaphorin7A (Sema7A) acts as an important mediator of these processes. Utilizing a semi-automated three-dimensional neurite tracing methodology and computational techniques, we have identified and quantitatively analyzed distinct topological features that shape the network in wild-type and Sema7A loss-of-function mutants. In contrast to those of wild-type animals, the sensory axons in Sema7A mutants display aberrant arborizations with disorganized network topology and diminished contacts to hair cells. Moreover, ectopic expression of a secreted form of Sema7A by non-hair cells induces chemotropic guidance of sensory axons. Our findings propose that Sema7A likely functions both as a juxtracrine and as a secreted cue to pattern neural circuitry during sensory organ development.

Semaphorin7A patterns neural circuitry in the lateral line of the zebrafish.

In a developing nervous system, axonal arbors often undergo complex rearrangements before neural circuits attain their final innervation topology. In the lateral line sensory system of the zebrafish, developing sensory axons reorganize their terminal arborization patterns to establish precise neural microcircuits around the mechanosensory hair cells. However, a quantitative understanding of the changes in the sensory arbor morphology and the regulators behind the microcircuit assembly remain enigmatic. Here, we report that Semaphorin7A (Sema7A) acts as an important mediator of these processes. Utilizing a semi-automated three-dimensional neurite tracing methodology and computational techniques, we have identified and quantitatively analyzed distinct topological features that shape the network in wild-type and Sema7A loss-of-function mutants. In contrast to those of wild-type animals, the sensory axons in Sema7A mutants display aberrant arborizations with disorganized network topology and diminished contacts to hair cells. Moreover, ectopic expression of a secreted form of Sema7A by non-hair cells induces chemotropic guidance of sensory axons. Our findings propose that Sema7A likely functions both as a juxtracrine and as a secreted cue to pattern neural circuitry during sensory organ development.

The Anatomy of the Thoracic Duct and Cisterna Chyli: A Meta-Analysis with Surgical Implications.

Background: The thoracic duct (TD) and the cisterna chyli (CC) exhibit a high degree of variability in their topographical and morphometric properties. Materials and Methods: PubMed, Scopus, Embase, Web of Science, Cochrane Library, and Google Scholar were searched to identify all studies that included information regarding the morphometric and topographical characteristics of the TD and CC. Results: The most frequent location of the TD termination was the left venous angle, with a pooled prevalence of 45.29% (95% CI: 25.51-65.81%). Moreover, the TD terminated most commonly as a single vessel (pooled prevalence = 78.41%; 95% CI: 70.91-85.09%). However, it divides into two or more terminating branches in approximately a quarter of the cases. The pooled prevalence of the CC was found to be 55.49% (95% CI: 26.79-82.53%). Conclusions: Our meta-analysis reveals significant variability in the anatomy of the TD and CC, particularly regarding TD termination patterns. Despite the predominance of single-vessel terminations, almost a quarter of cases exhibit branching, highlighting the complexity of the anatomy of the TD. These findings demonstrate the importance of detailed anatomical knowledge for surgeons to minimize the risk of accidental injury during head and neck, as well as thoracic surgeries. Our study provides essential insights that can enhance surgical safety and efficacy, ultimately improving patient outcomes.

Variations in the Termination of the Popliteal Artery: A Multidetector Computed Tomography Angiography (CTA)-Based Retrospective Study.

Comprehension of the intrucate anatomy and variations in the termination of the popliteal artery (PA) is increasingly essential for endovascular interventionists, plastic surgeons, vascular surgeons, and orthopedic surgeons, due to the rise in procedures like embolectomy, vascular grafting, free fibular flap surgery, and high-tibial osteotomy. Few studies from India have reported on the variant anatomy of PA termination, and none have used 128-slice tomography. This study aimed to observe the terminal branching pattern of the PA and the morphology of its terminal branches using 128-slice computed tomography angiography (CTA) and to analyze its relation to gender and laterality. A retrospective review of CTA images of 181 lower extremities from 100 patients (137 males and 44 females), aged five to 75 years, was conducted. The usual type I-A pattern was found in 75.69% of cases, while 24.31% exhibited variant patterns. Type III was the most common variation observed (19.34%), with type III-A being the most prevalent (11.05%). Types II-B and II-C were not observed. Among 84 bilaterally examined cases, 19.05% had unilateral variations and 15.48% had bilateral variations, with 8.33% showing bilaterally similar variations and 7.14% dissimilar variations. No significant difference in branching patterns was found between genders or sides. The mean length of the tibial-peroneal trunk (TPT) in the type I-A pattern was 3.00 ± 0.99 cm (right side: 3.21 ± 1.02 cm; left side: 2.82 ± 0.93 cm; males: 2.9 ± 1.00 cm; females: 3.37 ± 0.85 cm), with statistically significant differences between sides and genders. In the type II-A pattern, the mean TPT length was 7.16 ± 3.75 cm. An exceptionally long TPT (12.97 cm) was noted in one case of the III-B pattern. There is a high prevalence of variation in the termination pattern of the PA. Knowledge of these variations is crucial for any interventions in this region to avoid postoperative vascular complications and reduce patient suffering.

Differential cholinergic innervation of lemniscal versus non-lemniscal regions of the inferior colliculus

The inferior colliculus (IC), a midbrain hub for integration of auditory information, receives dense cholinergic input that could modulate nearly all aspects of hearing. A key step in understanding cholinergic modulation is to identify the source(s) and termination patterns of cholinergic input. These issues have not been addressed for the IC in mice, an increasingly important model for study of hearing. We examined cholinergic inputs to the IC in adult male and female mice. We used retrograde tracing and immunochemistry to identify three sources of cholinergic innervation of the mouse IC: the pedunculopontine tegmental nucleus (PPT), the laterodorsal tegmental nucleus (LDT) and the lateral paragigantocellular nucleus (LPGi). We then used Cre-dependent labeling of cholinergic neurons in normal-hearing ChAT-Cre mice to selectively label the cholinergic projections to the IC from each of the cholinergic sources. Labeling of cholinergic projections from the PPT and LDT revealed cholinergic axons and boutons terminating throughout the IC, with the ipsilateral projection being denser. Electron microscopic examination showed that these cholinergic axons can form traditional synaptic junctions with IC neurons. In separate experiments, selective labeling of cholinergic projections from the LPGi revealed bilateral projections to the IC. The LPGi axons exhibited relatively equal densities on ipsilateral and contralateral sides, but on both sides the terminations were largely restricted to the non-lemniscal regions of the IC (i.e., the dorsal cortex, lateral cortex and intercollicular tegmentum). We conclude first that cholinergic axons can form traditional synapses in the IC. In addition, lemniscal and non-lemniscal regions of the IC receive different patterns of cholinergic innervation. The lemniscal IC (IC central nucleus) is innervated by cholinergic neurons in the PPT and the LDT whereas the non-lemniscal “shell” areas of the IC are innervated by the PPT and LDT and by cholinergic neurons in the LPGi. Data AvailabilityData will be made available on request.

Evolving fill‐and‐spill patterns across linked early post‐rift depocentres control lobe characteristics: Los Molles Formation, Argentina

ABSTRACTInherited rift topography controls the sediment routing, timing of sand supply, and sedimentary linkage of early post‐rift depocentres. Exhumed examples of early post‐rift turbidite systems are rare and previous studies have examined the evolution of individual depocentres; in contrast, the detailed evolution of early post‐rift turbidite systems across multiple depocentres has never been documented. Current fill‐and‐spill models do not detail the stratigraphic architecture and evolution of sedimentological characteristics of multiple intraslope fans developed across topography, including bed type distributions. Here, the evolution of three intraslope fans that developed across two early post‐rift depocentres is documented along an 18 km long transect in the southern Neuquén Basin, Argentina. The relative chronology of sand supply in depocentres is constrained with new U–Pb ages, and sediment source areas with provenance analysis. The early post‐rift intraslope fans record progradation of the system and progressive sedimentary linkage of post‐rift depocentres, transverse to local syn‐rift structures, with sediment routing subparallel to the cratonic basin margin. The large‐scale stratigraphic architecture of intraslope fans indicates an evolution as a fill‐and‐spill system, with initial confinement through flow stripping and overspill to spillover with erosion and bypass across a transverse topographic high separating the depocentres. Changes in early post‐rift intraslope fan characteristics, including thickness, sandstone content, lobe complex stacking patterns, stratal termination patterns and bed type distribution, record changing confinement through time within a depocentre, and spatially across depocentres. The strong spatial and vertical stratigraphic variability of transitional flow deposits and hybrid event beds reflects enhanced erosion, sediment bypass and flow transformation across transverse relief between the two depocentres during the spillover phase. These findings advance current understanding of early post‐rift turbidite systems and refine fill‐and‐spill models, which will help the prediction of spatial and vertical changes in rock quality and connectivity in subsurface hydrocarbon reservoirs and CO2 storage sites.

Examining the dependence of southward shift of westerly anomalies on El Niño intensity: implications for varied El Niño termination patterns

Examining the dependence of southward shift of westerly anomalies on El Niño intensity: implications for varied El Niño termination patterns

A Review on Foliar Sclereids in Angiosperms

A review based on the data on foliar sclereids retrieved from earlier as well as recent publications has been prepared. An analysis of the account indicates that about 430 genera belonging to 110 families spread over 47 orders have foliar sclereids. The majority of taxa exhibit diffuse pattern of distribution of sclereids and terminal pattern has been recorded in 45 genera belonging to 25 families. Their morphogenic importance is also referred.

A Single Atrial Extrastimulation Resetting His Bundle During Supraventricular Tachycardia to Differentiate Atrial Tachycardia

BackgroundCatheter ablation is the curative treatment for paroxysmal supraventricular tachycardia (SVT). However, atrial tachycardia (AT) diagnosis is often challenging, especially when SVT is terminated by pacing. ObjectivesThis study sought to develop a novel method for AT diagnosis. MethodsA total of 147 SVTs including 28 ATs, 87 atrioventricular nodal re-entrant tachycardias, and 32 orthodromic reciprocating tachycardias were prospectively studied. Single atrial extrastimulation was performed at the proximal coronary sinus from a coupling interval 20 milliseconds shorter than the tachycardia cycle length and gradually decreased until the His bundle (HB) was first reset and further until the SVT was terminated. The response of the SVT during the first HB resetting and the termination pattern were examined. ResultsIn 27 of 28 ATs, tachycardia was unaffected when HB resetting whereas, in atrioventricular nodal re-entrant tachycardias or orthodromic reciprocating tachycardias (non-AT), tachycardia was simultaneously reset when HB resetting or was terminated with an atrio-Hisian block. When the coupling interval was further shortened for cases in which tachycardia persisted, all 33 SVTs with tachycardia termination with atrio-Hisian block were non-ATs, whereas 5 ATs and 7 non-ATs were terminated with Hisian-atrial block. The sensitivity, specificity, and positive and negative predictive values of the pattern of tachycardia that was unaffected when HB resetting for AT diagnosis were 96%, 100%, 100%, and 99%, respectively. Those of the pattern of tachycardia termination with atrio-Hisian block for non-AT diagnosis were 92%, 100%, 100%, and 42%, respectively. ConclusionsSingle atrial extrastimulation from the proximal coronary sinus during tachycardia was useful and effective for AT diagnosis.

The anterior saphenous vein. Part 2. Anatomic considerations in normal and refluxing patients. Endorsed by the American Vein and Lymphatic Society, the American Venous Forum, and the International Union of Phlebology

BackgroundThe term Anterior Accessory of the Great Saphenous Vein suggests this is a branch tributary vein despite this vessel’s anatomic features of a truncal vein. A multisocietal group suggested to designate this the anterior saphenous vein (ASV). This study was aimed to evaluate its ultrasound anatomy in normal and varicose limbs. MethodsThe clinical anatomy of the ASV was evaluated by narrative review of the literature. Additionally, the course of the ASV was evaluated in 62 limbs with no evidence of venous disease and 62 limbs with varicosities. ResultsThe ASV length, patterns of origin and termination are reported in both normal and patients with varicose veins. Discussion of the patterns is supported by the narrative review of the literature. ConclusionsThe ASV must be considered a truncal vein and its treatment modalities should be the same that for the great and small saphenous veins rather than a tributary vein.

The Anterior Saphenous Vein. Part 2. Anatomic considerations in normal and refluxing patients. Endorsed by the American Vein and Lymphatic Society, the American Venous Forum and the International Union of Phlebology.

The term Anterior Accessory of the Great Saphenous Vein suggest this is a branch tributary vein despite this vessel's anatomic features of a truncal vein. A multisocietal group suggested to designate this the Anterior Saphenous Vein (ASV). This study was aimed to evaluate its ultrasound anatomy in normal and varicose limbs. The clinical anatomy of the ASV was evaluated by narrative review of the literature. Additionally, the course of the ASV was evaluated in 62 limbs with no evidence of venous disease and 62 limbs with varicosities. The ASV length, patterns of origin and termination are reported in both normal and patients with varicose veins. Discussion of the patterns is supported by the narrative review of the literature. The ASV must be considered a truncal vein and its treatment modalities should be the same that for the great and small saphenous veins rather than a tributary vein.

Comprehensive analysis of splenic artery variations using computed tomography angiography: development of the IPALGEA classification system.

The splenic artery, an essential component of abdominal vascular anatomy, exhibits significant variations with clinical implications in surgical and radiological procedures. The lack of a standardized classification system for these variations hinders comparative studies and surgical planning. This study introduces the IPALGEA classification system, based on computed tomography angiography (CTA) findings, to address this gap. A retrospective analysis was conducted on 302 patients who underwent CTA at a tertiary university hospital between August 2021 and January 2022. The study focused on the evaluation of splenic artery variations, including the origin, course, terminal branching patterns, and the relationship between the inferior polar artery and the left gastroepiploic artery. The IPALGEA classification was developed to standardize the reporting of these variations. The study highlighted a significant prevalence of splenic artery variations, with the most common pattern being a superior course relative to the pancreas. The IPALGEA classification effectively categorized these variations, emphasizing the relationship between the inferior polar artery and the left gastroepiploic artery. The findings revealed that the bifurcation distance of the celiac trunk varied significantly between genders and that the presence of an inferior polar artery correlated with a shorter hilus distance. The IPALGEA classification offers a comprehensive and standardized approach to categorize splenic artery variations. This system enhances our understanding of abdominal vascular anatomy and has significant implications for surgical and radiological procedures, potentially reducing surgical complications and improving patient outcomes.

A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons

The brain controls nearly all bodily functions via spinal projecting neurons (SPNs) that carry command signals from the brain to the spinal cord. However, a comprehensive molecular characterization of brain-wide SPNs is still lacking. Here we transcriptionally profiled a total of 65,002 SPNs, identified 76 region-specific SPN types, and mapped these types into a companion atlas of the whole mouse brain1. This taxonomy reveals a three-component organization of SPNs: (1) molecularly homogeneous excitatory SPNs from the cortex, red nucleus and cerebellum with somatotopic spinal terminations suitable for point-to-point communication; (2) heterogeneous populations in the reticular formation with broad spinal termination patterns, suitable for relaying commands related to the activities of the entire spinal cord; and (3) modulatory neurons expressing slow-acting neurotransmitters and/or neuropeptides in the hypothalamus, midbrain and reticular formation for ‘gain setting’ of brain–spinal signals. In addition, this atlas revealed a LIM homeobox transcription factor code that parcellates the reticulospinal neurons into five molecularly distinct and spatially segregated populations. Finally, we found transcriptional signatures of a subset of SPNs with large soma size and correlated these with fast-firing electrophysiological properties. Together, this study establishes a comprehensive taxonomy of brain-wide SPNs and provides insight into the functional organization of SPNs in mediating brain control of bodily functions.