The patterns of puffing activity in the salivary gland polytene chromosomes have been studied during the late larval and prepupal stages of the medfly Ceratitis capitata. A total of 128 loci, with significant changes in puffing activity during this developmental period, were assigned to the five autosomes of the medfly. Two waves of puffing activity were observed, the first during the late larval stage and the second during the prepupal development. Overall puffing activity can be divided into four groups, group-IV activity being most conspicuous with 58 active loci. The major changes in puffing activity take place around jumping, a characteristic event occurring about 6 h before puparium formation, at puparium formation, and during midprepupal development. The overall puffing activity shows a positive correlation to the ecdysone titer in the hemolymph, suggesting that most of the changes in the activity of the puffs during the late larval and prepupal stages of the medfly may be regulated by ecdysone.Key words: polytene chromosomes, puffing patterns, ecdysone, Ceratitis capitata.