Extensive Pattern Research Articles

Origin of the Bohai Sea Basin, North China Craton, and implications for bidirectional back-arc extension in the East Asian continental margin

Abstract. The Bohai Sea Basin in eastern China is located in a back-arc extensional regime due to northwestward subduction of the Philippine Sea Plate and westward subduction of the Pacific Plate underneath the Eurasian Plate. The Bohai Sea Basin and surrounding region experience frequent earthquakes. Previous recognition of the origin of the Bohai Sea Basin was limited by the understanding of the back-arc extensional mode perpendicular to the subduction zone in the eastern Asian continental margin. In this paper, a new model for the genesis of the Bohai Sea Basin is proposed based on the construction of a major fault system and investigation of several main boundaries enclosing the Bohai Sea Basin. We have made field investigations and analyses of tectonic landforms and boundary faults on the northwest coast of the Bohai Sea and eastern and western margins of the Liaodong Peninsula, which revealed left-lateral strike-slip faults along the northwest coast of the Liaodong Bay and western margin of the Liaodong Peninsula. Then, we conducted a geological comparison of the Liaodong and Jiaodong Blocks and surrounding areas, as well as a structural interpretation of an aeromagnetic anomaly map of this region. We propose a right-lateral strike-slip fault between the eastern margin of the Liaodong Block and northwestern margin of the Jiaodong Block. This mode of movement may have resulted from the NE stretching, which is parallel to the subduction zone in the northwestern Pacific margin. Therefore, we suggest that the formation of the Bohai Sea Basin resulted from trench-parallel and trench-perpendicular extension. We speculate that the two-direction extension perpendicular and parallel to the subduction zone should be the basic pattern of the back-arc extension with a spherical geometric effect.

P0170 The presence of creeping fat in a TNBS-induced colitis model is associated with greater acute inflammation and bacterial translocation

Abstract Background The hyperplasia of the mesenteric adipose tissue in Crohn’s disease, known as creeping fat, is associated with transmural lesions and the translocation of microbial antigens. In a rat model of TNBS-induced colitis, 50% of the animals presented adipose hyperplasia. Macroscopic evaluations showed that these animals had a more advanced progression of the disease, and colonoscopy could predict the presence of fat two days before euthanasia (L Clua et al, ECCO, 2024). Methods Colitis was induced using TNBS (n=10; half female), with the animals being sacrificed after five days. Tissue from the lesion along with mesenteric adipose tissue was obtained for histopathological staining and evaluation (haematoxylin-eosin, Masson’s trichrome, and Gram). Samples of intestinal mucosa and mesenteric fat were preserved in RNAlater to assess the expression of inflammatory markers by qRT-PCR. Results were statistically compared between animals with and without creeping fat, including sex. Results In histopathological analysis, using a validated scoring system, animals with creeping fat showed significantly greater severity of acute inflammation at the transmural level, with an increase in cryptitis and a more pronounced and extensive fibrosis pattern. Additionally, colonies of Gram-positive cocci were detected in all animals with creeping fat. In contrast, animals without fat hyperplasia exhibited appreciable adipogenesis in only two individuals, with an average of 105 adipocytes per field, compared to 185 in animals with creeping fat. qRT-PCR revealed higher expression of Il1b, Il6, Il10, Nod2, and Tlr2 in the intestinal mucosa and hyperplastic mesenteric fat of animals with creeping fat. A significant increase in Tnfa was also observed in hyperplastic adipose tissue, while the expression of Tgfbr1 was higher in the intestinal mucosa of animals without creeping fat. No differences were found between sexes. Conclusion The results indicated a more intense acute inflammatory and fibrotic component in animals with creeping fat, consistent with the macroscopic observations. The hyperplasia and inflammation observed in the mesenteric adipose tissue may be provoked by bacterial translocation, whereas animals without hyperplasia present a component influenced by TGF-β1 activity that could help counteract the inflammation. References L Clua, R Suau, M Guasch, D Monfort-Ferré, I Vañó, C Serena, E Domènech, R Bartolí, J Manyé, P022 Development of a novel experimental model for studying creeping fat in Crohn’s Disease, Journal of Crohn’s and Colitis, Volume 18, Issue Supplement_1, January 2024, Pages i275–i276, https://doi.org/10.1093/ecco-jcc/jjad212.0152

Rightward brain structural asymmetry in young children with autism.

To understand the neural mechanism of autism spectrum disorder (ASD) and developmental delay/intellectual disability (DD/ID) that can be associated with ASD, it is important to investigate individuals at an early stage with brain, behavioural and also genetic measures, but such research is still lacking. Here, using the cross-sectional sMRI data of 1030 children under 8 years old, we employed developmental normative models to investigate the atypical development of gray matter volume (GMV) asymmetry in individuals with ASD without DD/ID, ASD with DD/ID and individuals with only DD/ID, and their associations with behavioral and clinical measures and transcription profiles. By extracting the individual deviations of patients from the typical controls with normative models, we found a commonly abnormal pattern of GMV asymmetry across all ASD children: more rightward laterality in the inferior parietal lobe and precentral gyrus, and higher individual variability in the temporal pole. Specifically, ASD with DD/ID children showed a severer and more extensive abnormal pattern in GMV asymmetry deviation values, which was linked with both ASD symptoms and verbal IQ. The abnormal pattern of ASD without DD/ID children showed higher and more extensive individual variability, which was linked with ASD symptoms only. DD/ID children showed no significant differences from healthy population in asymmetry. Lastly, the GMV laterality patterns of all patient groups were significantly associated with both shared and unique gene expression profiles. Our findings provide evidence for rightward GMV asymmetry of some cortical regions in young ASD children (1-7 years) in a large sample (1030 cases), show that these asymmetries are related to ASD symptoms, and identify genes that are significantly associated with these differences.

Topographic Evaluation of Inflammatory Periapical Lesions in the First Molar's Region Using CBCT.

Background: Investigating the pattern of extension in the periapical (PA) inflammatory lesions is important in the treatment plan and prognosis of treatment. Introduction: This study evaluated the topography of PA inflammatory lesions in the first molars using cone-beam computed tomography (CBCT). Methods: In this descriptive study, 197 CBCT images about patients in the age group of 14-77 years were analyzed. The maximum extension of the PA lesion in the three orthogonal planes related to the regions of maxillary and mandibular first molars was measured and reported in millimeters. Measurements were compared based on age, gender, dental arch, and root type. Statistical analysis was performed using percentages, repeated measure ANOVA, paired t-tests, and Pearson correlation coefficient. The significant level was set at 0.05. Results: The highest total mean lesion extensions were in the vertical plane followed by the buccolingual and mesiodistal plane. There was a statistically significant difference between the extension of the PA lesion in the vertical and mesiodistal (p < 0.001), vertical and buccolingual (p =0.001), as well as the mesiodistal and buccolingual planes (p =0.027). In the maxilla and mandible, the highest mean lesion extension was in the vertical, buccolingual, and mesiodistal plane, respectively. According to the root type, there was only a statistically significant difference in lesion extension in the buccolingual plane and between the mesial and distobuccal roots (p =0.030). Conclusion: Given the limitations of the present study, regarding the extension of the PA lesion in the first molar region, the bone structure of the maxilla and mandible follows a precise and delicate pattern. In this regard, future studies in different communities and races should be designed to address this issue in different communities. In addition, CBCT is a reliable imaging method to evaluate the extension of the PA lesion both morphologically and morphometrically.

Fatigue Life of Pre-Cut Seam Asphalt Mixture Composite Beams: A Combined Study of Fatigue Damage Evolution and Reflective Cracking Extension

This study investigated the impact of reflective cracking on the fatigue performance of asphalt pavements after milling and resurfacing under various conditions. Fatigue life was assessed through four-point flexural fatigue tests, while the crack extension pattern of composite beams was analyzed by digital image correlation (DIC) at both macroscopic and microscopic scales. Evaluation parameters such as stress ratios, immersion time, porosity, and types of viscous oils were assessed. A fatigue life prediction model of composite beams was established, accounting for the combined influence of these factors. To enhance the accuracy of determining composite beam failure, the critical fatigue damage was calculated by defining the damage variable in terms of the dynamic modulus. A nonlinear fatigue damage model was proposed, incorporating this critical damage under the combined influence of various factors. Additionally, a modified logistic function model was developed to describe the relationship between crack extension and failure life under different stress ratios, porosities, and viscous layer oil conditions. It was found that the modulus decay curves and the crack extension curves intersected at different stress levels as the life ratio increased. At the intersection, the modulus ratios were consistently around 0.55, marking the transition of the specimen from a stable to an unstable state. Beyond this point, the crack rapidly propagated, leading to a sharp reduction in the modulus until the specimen ultimately failed. Our results provide a basis for timing and conservation decisions.

Test–retest reliability and validity in lower limb proprioception tests using image capture technique in healthy adults

BackgroundProprioceptive assessments in clinical practice remain confined to ordinal scales, with a lack of ratio scales. Quantitative proprioception assessments such as threshold to detect passive motion (TDPM) and joint position sense (JPS) help determine the severity of functional impairments and evaluate treatment efficacy. However, their clinical introduction remains challenging due to the extensive and costly equipment required for their measurement.PurposeTo investigate the test–retest reliability and validity in the simplified TDPM and JPS using 14 image capture techniques in targeting hip, knee, and ankle joints.MethodsThis study included 16 volunteers. The measurements were conducted in individual rehabilitation rooms located within the wards of our hospital. Examiners measured a total of 12 TDPM and JPS, including hip, knee, and ankle joints, each performed three times. Moreover, the multi-joint TDPM, distinguished into lower limb flexion and extension patterns, was additionally performed. These measurements were repeated 1 week later. In statistical analysis, the test–retest reliability and construct validity were evaluated using intraclass correlation coefficients (ICC) and Spearman’s rank correlation coefficients.ResultsThe TDPM, multi-joint TDPM, and JPS (variable data; VE) demonstrated moderate reliability across all tested movement directions (TDPM: ICC 0.52–0.70, limits of agreement [LOA] < 10°; multi-joint TDPM: ICC 0.64–0.73, LOA < 10°; JPS: ICC 0.55–0.78, LOA < 10°). No significant correlation was observed between these tests.ConclusionsTDPM and JPS using image capture techniques can reliably measure proprioception and serve as independent tests to evaluate different aspects of proprioception. These findings contribute to future studies aimed at introducing quantitative proprioception tests into clinical practice and facilitating clinical decision-making for rehabilitation targeting proprioceptive impairment.

Prognostic Effect of Patterns of Local Extension and Satellitosis in cT3 Squamous Cell Carcinoma of the Oral Tongue: A Prospective Observational Study.

The anatomy of the tongue is three-dimensionally complex and is thought to play a central role in the local growth of oral tongue squamous cell carcinoma (OTSCC). Understanding patterns of tumor extension could improve a multimodal therapeutic approach. Thus, the main aim of this study was to provide a histological and microanatomical analysis of surgical specimens after compartmental surgery for OTSCC. The present prospective observational study included primary cT3 OTSCC (according to the eighth edition of the TNM classification) treated in an academic tertiary referral center with curative compartmental surgery, from July 2016 to July 2019. Analysis of histologic macrosections allowed assessment of standard pathologic parameters as well as a detailed analysis of the position of OTSCC cells from microanatomic and topographic standpoints. Of the 28 patients included, 71.4% were males, with a mean age of 64.9 years. Nine (32.1%) patients presented satellitosis, which was always located within the T-N tract. OTSCCs displaying satellitosis had a significantly higher median pathologic depth of invasion (DOI). A radiologic and pathological DOI > 15 mm significantly predicted the presence of satellites. There was a significant relationship between the presence of satellites and both positive lymph nodes and distant metastases. Approximately one-third of cases of intermediate-to-advanced OTSCC are characterized by tumor satellites located in the T-N tract. DOI exceeding 15 mm and the presence of clinically appreciable nodal metastases best predict the presence of satellitosis. Satellite-bearing OTSCC behave more aggressively, with an increased risk of distant metastasis and reduced survival.

The lawc gene emerged de novo from conserved genomic elements and acquired a broad expression pattern in Drosophila.

It has recently become evident that the de novo emergence of genes is widespread and documented for a variety of organisms. De novo genes frequently emerge in proximity to existing genes, forming gene overlaps. Here, we present an analysis of the evolutionary history of a putative de novo gene, lawc, which overlaps with the conserved Trf2 gene, which encodes a general transcription factor in Drosophila melanogaster. We demonstrate that lawc emerged approximately 68 million years ago in the 5'-untranslated region of Trf2 and displays an extensive spatiotemporal expression pattern. One of the most remarkable features of the lawc evolutionary history is that its emergence was facilitated by the engagement of Drosophilidae-specific short highly conserved regions located in Trf2 introns. This represents a unique example of putative de novo gene birth involving conserved DNA regions localized in introns of conserved gene. The observed lawc expression pattern may be due to overlap of lawc with the 5'-untranslated region of Trf2. This study not only enriches our understanding of gene evolution but also highlights the complex interplay between genetic conservation and innovation.

Identification of Fracture Extension Modes During Hydraulic Fracturing in Coalbed Methane Vertical Wells: A Case Study From the Southern Shizhuang Area of the Qinshui Basin, China

ABSTRACTThe accurate identification of fracture extension patterns in hydraulic fracturing can provide important guidance for the optimisation of fracturing parameters. In this paper, factors such as effective hole friction and wellbore flow friction during fracturing are fully considered, and a calculation model of net bottom‐hole pressure of fracturing is constructed. By introducing the change rate of net bottom‐hole pressure and the changing characteristics of the fracturing curve, seven fracture extension modes during hydraulic fracturing in coalbed methane vertical wells are established. The accuracy of the identification method is verified by the fracture monitoring and production results in Shizhuang South Block. The results show that fracture elongation is mainly controlled by in situ stress difference, angle between natural fracture and maximum principal stress, coal tensile strength, fracturing time, proppant and angle between other factors. When the fracture construction parameters are fixed, the smaller the difference between maximum and minimum horizontal principal stresses and the smaller the natural fractures and maximum horizontal principal stresses. When the reservoir potential is similar, the effective extension index is positively correlated with the gas production effect, and the effective extension index can effectively judge the fracturing effect. The higher the proportion of effective extension mode, the longer the extension time and the higher the stable daily gas production. The research results provide a method and reference for clearly identifying the fracture extension and the occurrence conditions of different extension modes in the hydraulic fracturing process.

Analysis of Semeru Volcano Deformation Using Static and Kinematic Solutions with GPS Data 2015-2023

Abstract Semeru Volcano is the highest peak on the island of Java. Semeru is characterized by continuous volcanic and strombolian eruptions that produce eruptive material which might result in casualties and infrastructure damage. One of them is the eruption on December 4, 2021. Therefore, to reduce the impact of future disasters, deformation monitoring using GPS is needed. In this study, we utilize GPS observation data located on Semeru Volcano and process it using GAMIT/GLOBK and TRACK. Processing is done with static solutions, kinematic solutions, and strain to determine the deformation pattern before, during, and after the December 4, 2021, eruption. The processing results of statis solution shows the pattern of displacement to the southeast in accordance with the direction of movement of the Sunda Block, with a range of 21.49 mm/year to 30.96 mm/year. The inSluence of the Sunda Block and other subduction-induced deformations were removed by referenced to CLUM station located 25 km from the volcano. The value of local velocity experienced a velocity of 1.81 mm/year to 7.83 mm/year with varying directions of movement. Kinematic processing using TRACK succeeded in showing the deformation pattern that occurred in the eruption of Semeru Volcano on December 4, 2021. Time Series in the Sirst phase (07:46:59 UTC–07:51:10 UTC) shows an inSlation and extension pattern of 0.054 m at the LEKR and 0.055 m at the SAWR station, followed by a deSlation and compression pattern of −0.018 m at the LEKR and −0.031 m at the SAWR in the second phase (07:51:10 UTC–07:56:00 UTC). The third phase (07:56:00 UTC– 08:03:00 UTC) showed deSlation and compression patterns with values of −0.058 m at the LEKR and −0.003 m at the SAWR. While using strain calculations, in the phase before the eruption, the strain was dominated by compression on the entire mountain body. Meanwhile, the phase after the eruption has a pattern that is divided into two, namely compression on the east side of the mountain and compression-extension on the southeast side of the mountain. These results are expected to contribute to understand the characteristics Semeru Volcano eruption in the future based on geodetic data.

Numerical simulation of hydraulic fracture extension patterns at interfaces of coal-measure rock strata and a new theoretical prediction model

Numerical simulation of hydraulic fracture extension patterns at interfaces of coal-measure rock strata and a new theoretical prediction model

High-Resolution 3D Shear-Wave velocity structure in xiong’an New Area, Beijing (China), revealed by short-period dense seismic array

The 3D shear-wave velocity (Vs) structure in the shallow crustal of Xiong’an New Area can reveal the sediment characteristics and the tectonic features of the blind faults. They are essential for earthquake hazard reduction and geothermal resource exploration in urban areas. The dense array detection method has proved to be an effective technology tool for obtaining the 3D Vs model of the urban area. Using the seismic ambient noise recorded by Xiong’an dense array, we separately conducted the frequency-Bessel transform method and the fast marching technology to estimate short-period (0.4–2.4 s) and long-period (2.5–6 s) fundamental Rayleigh wave phase velocity dispersions. By jointly inverting these dispersions, we finally obtain a fine shallow-deep (0–5 km) 3D Vs model. Our Vs structure correlates well with the tectonic units and reveals the overall stability of the shallow crust structure of the Xiong’an New Area. We extract 3D depths distribution characteristics of the Quaternary and Neogene bottom boundaries from 3D Vs model, which respectively indicates that the Xiongan New Area has been in a relatively stable tectonic position since the Cenozoic. We also provide new evidence to further determine the buried depth and extension patterns of the pre-existing faults or unconformable contact interfaces. Lastly, we analyze and summarize the 3D characteristics of structural sedimentary and potential geothermal exploration areas through conducting comprehensive analysis and interpretation of the regional terrestrial heat flow and geological drilling, which propose a new technical idea for the prospecting and assessing of the geothermal resources based on 3D Vs model.

Polymer-Injection Pilot in Colombian Field Indicates Reduced Carbon Footprint

_ This article, written by JPT Technology Editor Chris Carpenter, contains highlights of paper SPE 218169, “Carbon Footprint and Energy Intensity Assessment for Enhanced Oil Recovery (EOR) Based on Polymer Injection: A Colombian Case Study,” by Henderson I. Quintero, SPE, Pedro L. Solorzano, SPE, and Carolina Barbosa, Ecopetrol, et al. The paper has not been peer reviewed. _ Chichimene is an oil field in eastern Colombia. Since 2014, enhanced oil recovery (EOR) using a water-injection process has been performed successfully in part of this field. A polymer-injection pilot was executed over five injection patterns to optimize this process as a promising strategy for polymer-based EOR in challenging reservoir conditions with high temperatures and heavy oil. Field Background Included in Colombia’s energy-transition strategy are EOR technologies that, through the acceleration of oil production even with low energy consumption, increase the efficiency and carbon intensity of conventional oil-production processes. Several studies in the literature have reported improved energy and carbon indicators as a result of implementing EOR using thermal and chemical methods. Among these, polymer injection promises higher incremental oil production with lower energy consumption. The polymer-flooding (PF) pilot in the Chichimene field was performed to evaluate this technology’s ability to displace the heavy crude oil of the Fm1 formation (350–700 cp). The reservoir is more than 20 km long by 6 km wide and features 250–300 ft of thickness, with permeability between 100 md and 10 D and a depth of approximately 6,200 ft true vertical depth subsea. Polymer injection began at the end of 2015 in an extensive pattern (N1), followed by a smaller pattern (N2) in 2018 after a short water-injection stage. The polymer injection in medium patterns (N3 and N4) and a second small pattern (N5) began in 2019, being considered early chemical EOR because they were performed after the primary production stage. The well spacing of the patterns is 10 and 40 acres, respectively. The producer wells of each pattern are displayed in Table 1 of the complete paper. The PF project injected 21.65 thousand STB of polymer solution. At the end of 2022, incremental oil production was 4.9 billion bbl. At the time of writing, patterns show incremental recovery factors of between 4 and 9%. The reservoir showed strong signals of repressurization, gas/oil ratio reduction, water/oil ratio control, and oil incremental recovery. Energy consumption and carbon intensity were calculated for the oil production baseline case [waterflooding (WF) or primary production] and PF, considering the field’s energy matrix. The results were compared to quantify the effect of PF on these efficiency indicators. Evaluation of Product System Energy consumption was calculated for each phase involved in the processing system: lifting fluids, oil dehydration, water-clarification treatment, water reinjection, and PF. The evaluation did not include solvent or facilities to dilute the crude oil or the pumping energy used to deliver the oil to the pipeline system. Currently, the total volume of water produced is reinjected into the reservoir for pressure maintenance and oil-recovery purposes. Also, part of the produced gas is used in heaters and fire tubes for dehydration, but most is burned in a gas flare.

Anomalous venous collaterals in Apert and Crouzon syndromes and their relationship to ventricle size and increased intracranial pressure.

The exact association between the frequently present anomalous intracranial venous vasculature, emissary collaterals, ventriculomegaly, and increased intracranial pressure (ICP) in children with Apert and Crouzon syndromes remains an enigma. This study aimed to evaluate the association between the aberrant venous system and ventricle size and increased ICP, and to assess the development of the venous structures over time. This retrospective cohort study included all patients with Apert or Crouzon syndrome with available CT venography (CTV) scans of the brain. Anomalous venous vasculature was assessed by the total collateral score (TCS), which scored 9 intra- and extracranial venous structures (TCS range 0-16). Ventricle size (fronto-occipital horn ratio [FOHR]) was measured on the same scan. The presence of increased ICP, a shunt, tonsillar herniation, and head circumference were extracted from electronic patient records and were used as secondary covariates. Subsequent CTV scans were scored when available. Ninety patients were included in this study. The mean TCS was 7.5 ± 2.5, and was comparable for patients with Apert and Crouzon syndromes (mean 8.0 and 7.3, respectively). The presence of an extra abnormal venous structure was associated with an increase of the FOHR of 3.2% (p < 0.01). After dividing the venous structures into intra- and extracranial, a similar association between both and the FOHR was found (4.1% and 2.3%, respectively; p < 0.01). The TCS was similar for patients with normal and increased ICP at the time of the scan. Sixteen patients had dual CTV scans. The median time between both scans was 3.2 years. The presence of collaterals remained stable over time (median ΔTCS = 0.3). Seven patients with functioning shunts also maintained high TCSs (median TCS = 9). In patients with Apert and Crouzon syndromes, a close relationship between venous collaterals and ventricle size was observed, in which a more extensive aberrant venous drainage pattern, both intra- and extracranial, was associated with larger ventricles. Preliminary longitudinal data suggested that the presence of venous collaterals remained constant over time despite effective treatment of increased ICP, indicating reliance on these collaterals even in cases of normal ICP. The authors hypothesize that this dependence on collateral drainage is the result of their aberrant internal venous anatomy and predisposes individuals to increased ICP.

Prediction and Evaluation of Industrial Land Intensive Use: Adding Policy Impact to System Dynamics Model

Nowadays, urban development prioritizes improving quality over mere quantitative expansion, which requires a shift in land use from previous extensive use patterns to a more refined and efficient intensive use pattern. The intensive use of industrial land (IUIL) is a resource-utilization strategy for industrial land that aims at maximizing the dual growth of ecological and economic benefits by minimizing resource consumption and factor costs. In this research, IUIL is deconstructed into four aspects: input intensity, utilization intensity, economic benefits, and environmental benefits. Grounded in a qualitative analysis of the interconnected casual feedback among the various factors influencing IUIL, a system dynamics model with 36 indicators for IUIL evaluation and prediction is constructed. Policy impacts on IUIL from the supply side, utilization side, and supervision side are analyzed and embedded for a scenario simulation based on the theory of the entire life cycle of land use. The simulation shows its efficiency in reporting the variation tendency of industrial land use with varied policy impact strength. The results reveal a dynamic understanding of the evolving patterns of land intensification and present different characteristics of IUIL. This study further proposes policy recommendations to provide experience and references for the authorities to promote high-quality urbanization development in practice.

Bank vole genomics links determinate and indeterminate growth of teeth

BackgroundContinuously growing teeth are an important innovation in mammalian evolution, yet genetic regulation of continuous growth by stem cells remains incompletely understood. Dental stem cells responsible for tooth crown growth are lost at the onset of tooth root formation. Genetic signaling that initiates this loss is difficult to study with the ever-growing incisor and rooted molars of mice, the most common mammalian dental model species, because signals for root formation overlap with signals that pattern tooth size and shape (i.e., cusp patterns). Bank and prairie voles (Cricetidae, Rodentia, Glires) have evolved rooted and unrooted molars while retaining similar size and shape, providing alternative models for studying roots.ResultsWe assembled a de novo genome of Myodes glareolus, a vole with high-crowned, rooted molars, and performed genomic and transcriptomic analyses in a broad phylogenetic context of Glires (rodents and lagomorphs) to assess differential selection and evolution in tooth forming genes. Bulk transcriptomics comparisons of embryonic molar development between bank voles and mice demonstrated overall conservation of gene expression levels, with species-specific differences corresponding to the accelerated and more extensive patterning of the vole molar. We leverage convergent evolution of unrooted molars across the clade to examine changes that may underlie the evolution of unrooted molars. We identified 15 dental genes with changing synteny relationships and six dental genes undergoing positive selection across Glires, two of which were undergoing positive selection in species with unrooted molars, Dspp and Aqp1. Decreased expression of both genes in prairie voles with unrooted molars compared to bank voles supports the presence of positive selection and may underlie differences in root formation.ConclusionsOur results support ongoing evolution of dental genes across Glires and identify candidate genes for mechanistic studies of root formation. Comparative research using the bank vole as a model species can reveal the complex evolutionary background of convergent evolution for ever-growing molars.

The Impact of the EU Carbon Border Adjustment Mechanism on Chinas Exports and Suggestions on Countermeasures

The European Parliament plans to gradually introduce the Carbon Border Adjustment Mechanism in 2023, with a complete implementation set for 2026. As an essential trading partner of the EU, China will shoulder an additional carbon border tariff due to the lack of low-carbon technology and the dependence on extensive development patterns. These extra costs will undermine the profit margins of Chinas carbon-intensive industry sectors, possibly resulting in decreased exports and a restructuring of production. The latest estimate of the exact costs of Chinas embedded carbon exports to the EU is a problem that has received limited academic attention. Using the IPCC carbon emission accounting methodology and non-competitive single-region input-output analysis, this research precisely measures the embedded carbon in Chinas exports to the EU for the nine most affected industries. Furthermore, this study quantifies the impact of the CBAM on Chinas exports of these nine industry sectors. The findings suggest that China should improve the domestic carbon emission trading system and take the initiative to participate in formulating a carbon pricing consultation and dialogue mechanism, mitigating the negative impact of the EU CBAM on exports.

Thalamic functional dysconnectivity in patients with left-hemisphere chronic capsular and pontine stroke.

Through its extensive connection with the cortex, the thalamus constitutes the hub of cortico-subcortical circuits and participants in multi-dimensional functions. However, the differential involvements of thalamic functional connectivity in chronic capsular and pontine stroke are still unknown. The research recruited 66 left-lesion chronic stroke patients, including 46 capsular strokes (CS) and 20 pontine stroke (PS) patients, and 67 normal controls (NC). The thalamic subfields functional connectivities were compared between groups using a two-way repeated analysis of variance (ANOVA), corrected for confounders including age, gender, education and scanners. Spearman partial correlation was used to explore the potential association between altered thalamic FC and clinical variables. The ipsilesional thalamus of CS patients had abnormally decreased FC with widespread cognitive-related areas while increased FC with visual- and somatic-motor areas. In contrast, the ipsilesional thalamus of PS patients mainly demonstrated increased FC in these sensorimotor areas. Even in the contralesional thalamus, we observed similar (with the ipsilesional) but less extensive functional dysconnectivity patterns in both the CS and PS patients (P < 0.05, corrected using family-wise error [FWE] at the voxel level). Finally, we found significant group x subfields interactions on thalamic functional connectivity, where capsular vs. pontine stroke demonstrate varied functional dysconnectivity with specific thalamic subfields. Finally, a weak correlation was found between FC of both ipsilesional/contralesional thalamic subfields and motor, working and verbal memory. The thalamic functional dysconnectivity after chronic stroke are lesion-location and subfields dependent. Moreover, functional dysconnectivity were shown in both the ipsilesional and contralesional thalamus with similar patterns.

Dynamics of Bubble Plumes Produced by Breaking Waves

Abstract Bubble plumes play a significant role in the air–sea interface by influencing processes such as air–sea gas exchange, aerosol production, modulation of oceanic carbon and nutrient cycles, and the vertical structure of the upper ocean. Using acoustic Doppler current profiler (ADCP) data collected off the west coast of Ireland, we investigate the dynamics of bubble plumes and their relationship with sea state variables. In particular, we describe the patterns of bubble plume vertical extension, duration, and periodicity. We establish a power-law relationship between the average bubble penetration depth and wind speed, consistent with previous findings. Additionally, the study reveals a significant association between whitecapping coverage and observed acoustic volume backscatter intensity, underscoring the role of wave breaking in bubble plume generation. The shape of the probability distribution of bubble plume depths reveals a transition toward stronger and more organized bubble entrainment events during higher wind speeds. Furthermore, we show that deeper bubble plumes are associated with turbulent Langmuir number Lat ∼ 0.3, highlighting the potential role of Langmuir circulation on the transport and deepening of bubble plumes. These results contribute to a better understanding of the complex interactions between ocean waves, wind, and bubble plumes, providing valuable insights for improving predictive models and enhancing our understanding of air–sea interactions. Significance Statement This research contributes to understanding bubble plume dynamics in the upper ocean and their relationship with sea state variables. The establishment of a power-law relationship between the bubble penetration depth and wind speed, along with the association between whitecapping coverage and acoustic backscatter intensity, contributes to improved predictive capabilities for air–sea interactions and carbon dioxide exchange. The identification of the potential influence of Langmuir circulation on bubble plume dynamics expands our understanding of the role of coherent circulations in transporting bubble plumes. Additionally, this study presents a clear methodology using commercial sensors such as an ADCP, which can be easily replicated by researchers worldwide, leading to potential advancements in our comprehension of bubble plume dynamics.

CD44 facilitates adhesive interactions in airineme-mediated intercellular signaling.

Specialized cellular protrusions facilitate local intercellular communications in various species, including mammals. Among these, airinemes play a crucial role in pigment pattern formation in zebrafish by mediating long-distance Notch signaling between pigment cells. Remarkably, airinemes exhibit large vesicle-like structure at their tips, which are pulled by macrophages and delivered to target cells. The interaction between macrophages and Delta-ligand carrying airineme vesicles is essential for initiating airineme-mediated signaling, yet the molecular detail of this interaction remains elusive. Through high-resolution live imaging, genetic in vivo manipulations and in vitro adhesion assay, we found that adhesive interactions via the extracellular domain of CD44, a class I transmembrane glycoprotein, between macrophages and airineme vesicles are critical for airineme signaling. Mutants lacking the extracellular domain of CD44 lose their adhesiveness, resulting in a significant reduction in airineme extension and pigment pattern defects. Our findings provide valuable insights into the role of adhesive interactions between signal-sending cells and macrophages in a long-range intercellular signaling.