Since its discovery, nesfatin-1 (N1) has been recognised as an anorexigenic agent potentially related to obesity pathogenesis and development, including its modulatory effect on the brain's reward system and eating behaviours. As the results from human studies examining the relation between N1 serum levels, body mass index (BMI), and metabolic status are scarce and inconclusive, we aimed to investigate the association between serum N1 levels and obesity categories, obesity-related complications, and disturbed eating behaviour. We studied 110 patients with obesity divided into obesity categories according to their BMI and metabolic status. N1 was measured in a fasting state (N10) and 2h after a glucose load (N12) and correlated with anthropometric measurements, serum analysis, and the presence of selected obesity-related complications. Neither N10 nor N12 correlated significantly with obesity; however, N10 tended to be high in patients with a high BMI. A positive correlation was observed among N12, fat-free mass (p = 0.022), and muscle mass (p = 0.02). We found positive correlations between N10 and N12 with aspartate aminotransferase (p = 0.012 and p = 0.022, respectively) and alanine aminotransferase (p = 0.027 and p = 0.006, respectively). Patients with dyslipidaemia had significantly higher N10 (p = 0.03) and N12 (p = 0.049) levels. Neither N10 nor N12 correlated significantly with disturbed eating behaviour; however, low N10 levels were associated with a hedonic eating pattern (p = 0.03). N1 may be involved in the pathogenesis of obesity and obesity-related complications; however, owing to the complex mechanisms of its secretion and action, further clinical and experimental research is needed.
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