Deposition Patterns Research Articles

Comparative Morphology of Skeletal Development in Homo sapiens and Raja asterias: Divergent Stiffening Patterns Due to Different Matrix Calcification Processes.

Before calcification begins, the early embryonic and fetal skeletal development of both mammalian Homo sapiens and the chondrichthyan fish Raja asterias consists exclusively of cartilage. This cartilage is formed and shaped through processes involving tissue segmentation and the frequency, distribution, and orientation of chondrocyte mitoses. In the subsequent developmental phase, mineral deposition in the cartilage matrix conditions the development further. The stiffness and structural layout of the mineralized cartilage have a significant impact on the shape of the anlagen (early formative structure of a tissue, a scaffold on which the new bone is formed) and the mechanical properties of the skeletal segments. The fundamental difference between the two studied species lies in how calcified cartilage serves as a scaffold for osteoblasts to deposit bone matrix, which is then remodeled. In contrast, chondrichthyans retain the calcified cartilage as the definitive skeletal structure. This study documents the distinct mineral deposition pattern in the cartilage of the chondrichthyan R. asterias, in which calcification progresses with the formation of focal calcification nuclei or "tesserae". These are arranged on the flat surface of the endo-skeleton (crustal pattern) or aligned in columns (catenated pattern) in the radials of the appendicular skeleton. This anatomical structure is well adapted to meet the mechanical requirements of locomotion in the water column. Conversely, in terrestrial mammals, endochondral ossification (associated with the remodeling of the calcified matrix) provides limb bones with the necessary stiffness to withstand the strong bending and twisting stresses of terrestrial locomotion. In this study, radiographs of marine mammals (reproduced from previously published studies) document how the endochondral ossification in dolphin flippers adapts to the mechanical demands of aquatic locomotion. This adaptation includes the reduction in the length of the stylopodium and zeugopodium and an increase in the number of elements in the autopodium's central rays.

Non-uniform dune development in the presence of standalone beach buildings

Shoreward sand transport and dune development are increasingly influenced by the urbanization of beach-dune systems in the Netherlands. Three topographic datasets, on various spatio-temporal scales, are used to study the effect of standalone buildings on long term local dune development. On the smallest scale, terrestrial laser scans are used to study the geomorphological effects of two sea containers on the beach. On the intermediate scale, the geomorphological effects of a beach pavilion on the local dune development are studied with a 2-year topographic dataset of (bi) monthly permanent laser scans. Finally, 15 yearly airborne lidar scans of the beach-dune system in Noordwijk are used to evaluate the effect of multiple beach pavilions on dune growth variations. The small-scale experiment shows that horseshoe-shaped deposition patterns developed on the leeside of the containers. These depositions follow daily wind changes and leave deposits corresponding to the residual wind direction over the whole measuring period. Similar patterns are found around the larger beach pavilion, but anthropogenic activities like bulldozing and beach shaping make the determination of the effect on dune development harder to discern. Evaluation of the longer-term dataset reveals large variations in dune height and volume around beach pavilions. Dune height/volume increases vary between 1 and 8 m in height and 0–200 m3 in volume. A variability analysis shows that the length scale of alongshore variability in dune height/volume of urbanized dunes can be 10 times smaller than for natural dunes. For about half the beach pavilions, variations in dune height and volume are significantly correlated to the location of beach pavilions but correlation to particular beach pavilion properties is yet inconclusive.

Outburst flood events since the Last Glacial Maximum in the Hutiao Gorge of Jinsha River: Geomorphological evidence from eddy gravel bars

Breaching of natural dams in the Hutiao Gorge of the Jinsha River across the Yulong-Haba Mountains is one of the major geomorphological events in the largest river of Asia. Catastrophic flood events have an important influence on the geomorphological evolution of the First Bend of the Yangtze River since the Last Glacial Maximum (LGM). However, detailed geomorphological evidence, ages, and depositional phases of outburst flooding remain unclear. In this paper, large-scale gravel bars overlying the river terraces and dammed lakes are analyzed within the Daju Basin. The macroscopic geomorphological characteristics of megaflood landforms with large thickness and uniform gravel bars are similar to those of Missoula and Altai megafloods. Morphological analysis shows that eddy gravel bars (EGBs) with rhythmic structure are characterized by a dome-shaped protrusion in the cross section and a gravel mound in the longitudinal section. Each bar with oblique, parallel and massive bedding was analyzed from the horizontal and vertical directions. Three dome-shaped bars clearly show at least three palaeoflood phases. Quartz grain surface macrotextures are mainly V-shaped pits and conchoidal fractures, which exhibits a high-energy environment during the flood. The stratigraphic sequence model of EGBs is dominated by coarse sand and fine gravel rhythmic bedding in high-energy eddy environments and suspended sand deposits in low-energy backwater environments. Such phenomenon reveals megaflood fluctuates, which is also confirmed by geochemical difference. The episode of natural dam break floods triggered by tectonic activity and glacier fluctuation was dated to 20–17 ka during the LGM. Sedimentary pattern of EGBs offers fresh insights into understanding the megaflood geomorphological characteristics of the upper Yangtze River and better identification similar high-energy palaeoflood landforms in the southeast Tibetan Plateau.

Leaf micromorphology and anatomical traits of leaves as potential taxonomic markers for infrageneric classification of<em> Cinnamomum</em> (Lauraceae)

This study aimed to examine the leaf anatomy of the eight species of Cinnamomum (family Lauraceae) that occur in Sri Lanka to evaluate its potential utility in taxonomic delimitation. Leaf surface micromorphology and internal anatomy were studied using standard light and scanning electron microscopy. Infrageneric and interspecific variation in micromorphology of cuticular materials was observed in both abaxial and adaxial surfaces. Both abaxial and adaxial surfaces had undulating, thick cuticles with different patterns of wax deposition. Leaves were hypostomatic with no special arrangement for epidermal pavement cells. Simple, unicellular, unbranched, solitary and non-glandular trichomes were observed in different species on both surfaces of the leaves. Both adaxial and abaxial surfaces in C. dubium and C. capparu-coronde were densely covered with simple, unicellular/ falcate, long and thin trichomes. Transverse sections (TS) of the leaves were different in shape (symmetrical, asymmetrical, irregular, saucer). The midrib contained one open arch central vascular bundle that was different in shape (oval, elongated, irregular, ‘V’shaped, partially dissected into 2 or 3 segments) in different species. The number of collateral vessels was dissimilar between the species and varied in number (±10 - ±17). Isolated or aggregated crystals were observed in different parts of the leaf and oval-to-round schizogenous secretory cells were present in C. capparu-coronde, C. citriodorum and C. dubium. Leaf cuticular features together with the presence/absence of secretory cavities, trichome shape and density, midrib cross-section outline and shape of vascular bundles are taxonomically informative characteristics that can be used to differentiate the eight species of Cinnamomum that occur in Sri Lanka.

PH effects on capture efficiency and deposition patterns in sessile droplet immunoassays: An XDLVO analysis.

Immunosensors are crucial for various applications, with capture efficiency and detection time as key performance parameters. Sessile droplets on functionalized substrates have demonstrated potential as micro-reactors for antibody-antigen binding, reducing detection time and analyte volume due to the presence of convective currents. Tuning the surface charges by adjusting buffer pH can modulate antigen capture efficiency. While the impact of pH has been studied on antibody-antigen binding in flow and non-flow systems, the use of sessile droplets and the specific impact of buffer pH on the capture efficiency of surface-functionalized antibodies remains understudied. Understanding how pH affects capture and deposition patterns is vital for optimizing immunosensor design. Additionally, the mechanisms governing internal flow within the droplet and dominant driving forces require further investigation. We investigated the effect of varying buffer pH on prostate-specific antigen (PSA) capture by anti-PSA functionalized polydimethylsiloxane substrates. Capture efficiency was measured using the Brown-Anson model applied to cyclic voltammetry, validated with electrochemical impedance spectroscopy. pH significantly influenced PSA capture by surface-immobilized anti-PSA IgG. The extended Derjaguin-Landau-Verwey-Overbeek theory explained the interplay between pH and internal flow. Micro-particle image velocimetry (PIV) confirmed internal flow, primarily driven by Marangoni flow from solute concentration gradients. Controlling buffer pH in biosensors offers higher capture efficiency and desired deposition patterns. These insights advance immunosensor design and hold potential for biomedical and diagnostic applications.

Sedimentation and photophoretic levitation of aerosol clusters in the free molecular regime

Sedimentation of fractal aerosol clusters in rarefied gas medium in the dark and under external illumination similar to sunlight is studied in a numerical experiment taking into account orientation effects, depending on fractal dimension varying within a broad range. The calculation of forces and their moments, including friction forces and photophoretic forces, was carried out on the basis of approximation of free molecular gas kinetic regime and previously developed Monte-Carlo algorithms. Calculation involved 10000 clusters, each of them containing 160 primary spherical particles and efficiently absorbing sunlight and IR radiation, similarly to the particles of soot aggregates.The velocity of multi-particle cluster settling in the absence of illumination, when particle temperatures are equal to the temperature of the gaseous environment, is a distinct function of fractal dimension and is rather close to the velocity of settling of single spherical particles. The settling velocity approximation depending on fractal dimension has been obtained.Illumination brings dramatic changes into sedimentation pattern. The range of cluster sedimentation rate variations broadens substantially, which is a manifestation of gravito-photophoretic effect, so that some clusters even start to levitate. The proportion of levitating clusters is essentially dependent on fractal dimension. For soot-like clusters under irradiation similar to sunlight and pressure equal to atmospheric at the altitude of 30 km, the proportion of levitating aggregates is approximately 7%, which is in good agreement with the experimental data. In the dark and under irradiation, the aggregates rotate, and their trajectories are shaped as spirals.In the context of photophoretic phenomena, a result of principle has been obtained: under irradiation with sunlight, gravito-photophoretic levitation of aerosol soot-like aggregates composed of identical primary particles is possible at a pressure corresponding to the stratospheric altitudes. This result points to the possibility of substantial influence of gravito-photophoretic effect on transport and localization of soot aerosol in the Earth’s stratosphere and mesosphere.

Immunolocalization of hordein synthesis and transport in developing barley endosperm.

The spatial accumulation of hordeins in the developing endosperm of barley grains was examined by immunofluorescence microscopy (immunolight microscopy [iLM]) and immunoelectron microscopy (iEM) to establish the timing and subcellular pattern of hordein synthesis and deposition. The pattern seen for hordeins was compared to other abundant grain proteins, such as serpin Z4 and lipid transfer protein 1 (LTP1). Hordein accumulates throughout grain development, from 6 to 37 days post-anthesis (DPA). In contrast, serpin Z4 was present at 6 DPA, but the greatest synthesis and accumulation occurred during the middle of seed development, from 15 to 30 DPA. LTP1 accumulated later in seed development, from 15 to 30 DPA. Hordeins accumulated within the lumen of the endoplasmic reticulum (ER), were exocytosed from the ER membrane, and accumulated in protein bodies, which then fused either with the protein storage vacuoles or with other protein bodies, which also later fused with the protein storage vacuoles. iEM showed hordein, and LTP1 appeared not to traverse the Golgi apparatus (GA). Hordein, LTP1, and serpin Z4 colocalized to the same protein bodies and were co-transported to the protein storage vacuole in the same protein bodies. It is likely that this represents a general transport mechanism common to storage proteins in developing grains.

Reconstruction of the Dynamics of a Catastrophic Crater Lake Outburst Flood, Changbaishan‐Tianchi Volcano

AbstractReconstruction of the catastrophic drainage following the Millennium Eruption (ME) of Changbaishan‐Tianchi volcano in 946 ± 20 CE is of great significance, as it contributes to improving the regional maximum flood record and develop rare flood risk analysis. However, limited knowledge exists concerning the failure mode, magnitude, and transport processes of the outburst flooding. In this work, we present a whole system model that describes the paleohydrology of catastrophic drainage using geological records along the downstream valley. The model encompasses the crater lake dynamics, an approximation of the breach erosion process and flood propagation downstream. The boulder competence method was used to constrain by reasonable flow parameters, while mitigating the uncertainty caused by the ambiguous geological paleostage indicators. Paleohydrologic analysis indicates that at least 1 km3 of water was released from the caldera, with the vertical breach erosion rates as high as 34 m/hr. Volcanic activity during the ME II may have directly contributed to triggering of the flood event. The local hydrodynamic response of the downstream riverbed captures the dynamic migration patterns of sediments across spatio‐temporal scales, offering a comprehensive interpretation of the specific scouring surfaces observed in the geological profile. The analysis of simulated inundation boundaries reveals that not all recorded inundations can be attributed to the crater lake outburst event. Reconstructions of megafloods based on downstream constraints on flood stage, velocity and discharge can help to infer and constrain the dynamics of dam failure mechanisms, and also contribute to our understanding of these complex paleohydrologic events.

Environmental impact assessment based on particulate matter, and chlorophyll content of urban trees

The amount of dust deposited on tree leaves is a cost-effective indicator of air quality. Our aim was to explore the leaf surface deposition, and chlorophyll content of leaves along a road section that started at an intersection, and ended in a less disturbed suburban area in Debrecen, Hungary. We also assessed the impact of meteorological conditions on the amount of deposited dust. Leaf samples were collected in July, and September 2022 from Celtis occidentalis, a frequent species in green urban areas of Debrecen. We found a significant negative correlation between dust deposition, and the distance from the intersection in July. In September, dust deposition decreased considerably compared to July, due to rainfall before the second sampling. Surprisingly, we found a positive correlation between dust deposition and chlorophyll content in July. Our findings suggest that dust deposition on leaves serves as a reliable indicator of traffic intensity, because the excess dust caused by the proximity of vehicle traffic can be detected on the leaf surface. Although, rainfall can disrupt the patterns in dust deposition that have developed over an extended period through wash-off and resuspension. Hence, it is advisable to consider these effects while selecting the sampling time and evaluating the results.

Fractal dimension and its implication to mineral exploration: a case study from the Jonnagiri and Gadag gold deposits in India

Fractal dimensions characterize the spatial distribution pattern of mineral deposits, allowing the mineralization processes beneath and at the surface to be understood. This study considered a mineralized vein along the fracture/shear zone near the Dona Temple in the Jonnagiri gold deposit and borehole data from the Sangli Block in the Gadag gold deposit. The above-mentioned zones were considered using fractal dimensional analysis to constrain the fluid-flow pathways. The box-counting technique was used to measure the fractal dimension of the mineralized vein along the surface from a detailed to a regional scale. The fractal correlation integral method was used to provide the fractal dimension values from the gold assay (in g t −1 ). The box-counting fractal dimension values obtained varied from 1.07 to 1.36, whilst the fractal dimensions obtained from the fractal correlation integral method ranged from 0.03 to 1.97. Higher fractal values signify a fully matured interlinked fracture/fault network with steep fluid-flow pathways, while lower values indicate the converse condition. Further, the study showed that the fractal analysis of the mineralized vein along the fracture/shear zone appeared to correlate with the fault system rather than with the litho-contact. Economic gold deposition occurs mainly along a structure with a steep fractal gradient, which is the target area for economic gold exploration.

Stratigraphic-chemostratigraphic assessment of early dolomitization and associated phosphogenesis of the Ediacaran-Cambrian Salitre Formation, Irecê Basin, Brazil

During the Precambrian − Cambrian transition, the occurrence of phosphogenesis and the rise in atmospheric oxygen levels are closely linked to the proliferation of life. However, the mechanisms associated with these phenomena and their underlying causes are not well understood. The Irecê Basin represents the remnants of intracratonic marine environments that document the Neoproterozoic sea flooding of West Gondwana. This study presents an integrated analysis of sedimentary facies and chemostratigraphy through high-resolution sampling of pristine limestones and dolomites from the Salitre Formation in east-central Brazil. The aim is to explore the relationship between environmental settings and phosphatized stromatolites by combining detailed facies descriptions with isotopic and geochemical data. A total of fifteen lithofacies types are identified and grouped into seven major facies associations. These associations reveal a depositional stacking pattern defined by flooding surfaces and transgressive–regressive cycles, allowing for the division of the Salitre Formation into two depositional sequences. The basal sequence includes sedimentary phosphorite deposits intimately associated with widespread dolomitization levels at the top. Here, we describe a combination of two major processes for the dolomitization model of the Salitre Formation, with a contribution of evaporative environment settings related to a sabkha, and the influence of a meteoric mixing zone. Furthermore, three stages of phosphogenesis have been identified, with two of them interpreted as early diagenetic deposition associated with stromatolites and early cementation, and the last one associated with a late stage of phosphogenesis. The combined analysis of REY patterns, 87Sr/86Sr ratios, and geochemical results suggests a significant continental influence on both dolomitization and phosphogenesis. The identification of depositional settings and stratigraphic patterns for these deposits is crucial for the understanding of phosphogenesis during the Ediacaran, which may have been influenced by the continentalization of the marine environments forced by the intracratonic marine setting and the tectonic evolution at the end of the Neoproterozoic Era.

From Bottom‐Water Production to Warm Water Intrusions—The Cenozoic History of Bottom‐Current Evolution Offshore the Denman‐Shackleton Region, East Antarctica

AbstractThe deep glacial trough and hinterland of the Denman Glacier (East Antarctica) makes the area around the Shackleton ice shelf sensitive to ice loss due to warmer deep water intruding onto the continental shelf in the near future. In addition, the configuration of the ocean currents offshore is an important factor in priming the local and regional vulnerability to warm water intrusions. Here, we use reflection seismic data sets from the Bruce Rise offshore the Denman‐Shackleton region to investigate the Cenozoic history of the ocean bottom current configurations offshore and their influence on the Cenozoic sedimentation patterns of the Denman‐Shackleton region. On the Bruce Rise, sediment drift building, and erosional features indicate three distinct ocean current configurations, (a) the production of dense shelf waters in times of a smaller East Antarctic Ice Sheet (EAIS), (b) periods dominated by a strong Antarctic Slope Current (ASC) and (c) periods with a weak ASC. During the early establishment of the EAIS, the Denman‐Shackleton area contributed to the production of Antarctic Bottom Water, a process which stabilizes the regional ice sheet. With a growing icesheet, the ASC strengthened representing an effective barrier between the continental shelf and the warmer water masses of the deeper ocean during most of the times of an extended EAIS. The transition in the paleoceanographic setting from a strong, erosive ASC, toward a weak ASC increases the vulnerability of the Denman‐Shackleton continental shelf to deep water intrusions as we are observing today.

Pathogen shape: Implication on pathogenicity via respiratory deposition

The shape of environmental aerosols contributes to the discrepancy in their dynamic behavior compared to spherical particles, which have received inadequate consideration. We reported deposition patterns of aerosols and aerosol-transmissible pathogens in real human respiratory systems, taking into account their actual shape, using a validated computational-based model. We found that the shape of the aerosols significantly influenced its deposits and accessibility within the respiratory system, significantly in the tracheobronchial region. As an example, we estimated that over 180 % of differences in deposits in the trachea and bronchi were attributable to pathogens shape, inferring the underlying pathogenicity difference of these regions. These findings, capturing the spatial heterogeneity of pathogens and aerosols deposition in human respiratory system, have major implication for understanding the evolution of aerosol-related disease.

Atmospheric deposition patterns in bulk open field precipitation and throughfall in Aleppo pine forest and black pine forest on the eastern Adriatic coast

The Mediterranean region, with its unique ecological characteristics, is particularly sensitive to global environmental changes, including climate change and impact of air pollution.Although Aleppo pine and black pine forests are the most abundant on the eastern Adriatic coast, atmospheric deposition in these forests is poorly studied. Changes in the chemical composition of precipitation as it passes through the tree canopy can lead to soil and groundwater eutrophication, and soil acidification, which affects plant vitality. In this study, the dynamics of ion deposition in Aleppo pine forest (Pinus halepensis Mill.) and black pine forest (Pinus nigra Arnold) on the eastern Adriatic coast are investigated, focusing on throughfall and bulk open field depositions.The aim of our research was to fill the gaps in understanding the influence of tree canopies on deposition fluxes in two different Mediterranean pine stands and to compare total inorganic nitrogen loads with critical loads. Over a period of two years, bulk open field precipitation and throughfall were sampled, measured and analysed using the ICP Forest methodology.The results indicate significant differences in ion deposition between bulk open field and throughfall, with throughfall showing higher values for almost all ions. The highest enrichment ratio was determined for K+. The comparison of the actual inorganic nitrogen load with the critical nitrogen load for Mediterranean pine forests revealed that the inorganic nitrogen load exceeded the critical load in the Aleppo pine forest. Ion deposition increased in the throughfall compared to bulk precipitation, which can be attributed to the seasonality of precipitation, including leaching and long dry periods. These findings enhance our understanding of ion deposition fluxes in vulnerable Mediterranean pine ecosystems and emphasize the need for long-term research on this topic in the actual changing environmental conditions.

Fibronectin isoforms promote postnatal skeletal development

Fibronectin (FN) is a ubiquitous extracellular matrix glycoprotein essential for the development of various tissues. Mutations in FN cause a unique form of spondylometaphyseal dysplasia, emphasizing its importance in cartilage and bone development. However, the relevance and functional role of FN during skeletal development has remained elusive. To address these aspects, we have generated conditional knockout mouse models targeting the cellular FN isoform in cartilage (cFNKO), the plasma FN isoform in hepatocytes (pFNKO), and both isoforms together in a double knockout (FNdKO). We used these mice to determine the relevance of the two principal FN isoforms in skeletal development from postnatal day one to the adult stage at two months.We identified a distinct topological FN deposition pattern in the mouse limb during different gestational and postnatal skeletal development phases, with prominent levels at the resting and hypertrophic chondrocyte zones and in the trabecular bone. Cartilage-specific cFN emerged as the predominant isoform in the growth plate, whereas circulating pFN remained excluded from the growth plate and confined to the primary and secondary ossification centers. Deleting either isoform independently (cFNKO or pFNKO) yielded only relatively subtle changes in the analyzed skeletal parameters. However, the double knockout of cFN in the growth plate and pFN in the circulation of the FNdKO mice significantly reduced postnatal body weight, body length, and bone length. Micro-CT analysis of the adult bone microarchitecture in FNdKO mice exposed substantial reductions in trabecular bone parameters and bone mineral density. The mice also showed elevated bone marrow adiposity. Analysis of chondrogenesis in FNdKO mice demonstrated changes in the resting, proliferating and hypertrophic growth plate zones, consistent alterations in chondrogenic markers such as collagen type II and X, decreased apoptosis of hypertrophic chondrocytes, and downregulation of bone formation markers. Transforming growth factor-β1 and downstream phospho-AKT levels were significantly lower in the FNdKO than in the control mice, revealing a crucial FN-mediated regulatory pathway in chondrogenesis and bone formation.In conclusion, the data demonstrate that FN is essential for chondrogenesis and bone development. Even though cFN and pFN act in different regions of the bone, both FN isoforms are required for the regulation of chondrogenesis, cartilage maturation, trabecular bone formation, and overall skeletal growth.

Deposition kinetics and adhesion behavior of polydopamine-based capsules on cellulose fibrous substrates

Polydopamine (PDA)-based capsules have been broadly used to functionalize cellulose-based fabrics owing to their enhanced adhesion properties. However, the deposition kinetics and adhesion behavior of PDA-based capsules on the fabrics remain understudied. In this work, the sizes, concentrations, and deposition time of PDA particles, as well as the electrostatic force between PDA particles and the fabrics were investigated to study the deposition kinetics and adhesion behavior of PDA particles on Lyocell fibers. The introduction of electrostatic interaction significantly enhanced both the deposition amount and the uniformity of PDA particles onto the fiber surface. The deposition kinetics of PDA particles on the fibers could be accurately described using a modified collision model, with the coverage of PDA particles conforming to a monolayer deposition pattern. The adhesion behavior of PDA particles was investigated by molecular dynamics simulation and isothermal titration calorimetry. The results indicated the driving forces during the deposition process were mainly hydrogen bonds, electrostatic force and van der Waals force. The van der Waals force was increased by around 106 %, and electrostatic force was even increased by approximately 201 % after the fiber cationization. This study provided a theoretical foundation for achieving controllable and quantitative surface functionalization of cellulose fibrous substrates.

Co-flowering richness has variable effects on pollen quantity and quality limitation in four Clarkia species.

Pollination failure occurs from insufficient pollen quantity or quality. However, the relative contributions of pollen quantity vs quality to overall pollen limitation, and how this is affected by the co-flowering context, remain unknown for most plant populations. Here, we studied patterns of pollen deposition and pollen tube formation across populations of four predominately outcrossing species in the genus Clarkia to evaluate how richness of co-flowering congeners affects the contribution of pollen quantity and quality to pollen limitation. We partition variation in pollen deposition and pollen tube production across individuals, populations and species to identify the main sources of variation in components of reproductive success. We further quantify the relative contribution of pollen quantity and quality limitation to the reproductive success of the four Clarkia species using piecewise regression analyses. Finally, we evaluate how variation in the number of co-flowering Clarkia species in the community affects the strength of pollen quality and quality limitation. Across all contexts, pollen deposition and the proportion of pollen tubes produced varied greatly among individuals, populations, and species, and these were not always correlated. For instance, C. xantiana received the smallest pollen loads yet produced the highest proportion of pollen tubes, while C. speciosa exhibited the opposite pattern. Yet, co-flowering richness had variable effects on the strength of pollen quantity and quality limitation among populations. Specifically, breakpoint values, which are an indicator of overall pollen limitation, were two times larger in the four-species community compared with one and two-species communities for two Clarkia species, suggesting that pollen limitation can increase with increasing richness of co-flowering congeners. Our results reveal a complex interplay between quantity and quality of pollen limitation and co-flowering context that may have different evolutionary outcomes across species and populations.

Enhancing prognostic guidance in renal light-chain amyloidosis: a new staging system incorporating pathological characters.

Advancements in treatment regimens have led to improved outcomes in renal Immunoglobulin light-chain amyloidosis. Nevertheless, a subset of patients may still experience renal adverse events despite achieving hematologic very good partial response or better. This discrepancy may be attributed to the deposition pattern of amyloid in renal tissue. To enhance prognostic assessment, a staging system that incorporates both pathological characteristics and clinical indicators should be developed. Patients newly diagnosed through renal biopsy between January 1, 2017, and December 31, 2022, were included. The renal pathology of patients was evaluated according to amyloid score (AS). Risk factors for end-stage renal disease or renal progression were identified by thecompeting risk model, then to develop a renal staging system. The Concordance index (C-index), internal cross-validation and Decision Curve Analysis (DCA) were used to evaluate the performance of the new staging system. 74 patients were included, and 16 (21.6%) patients had end-stage renal disease or renal progression within 24.7 (11.9, 50.7) months. AS and estimated glomerular filtration rate (eGFR) were identified as independent risk factors and the staging system based on them, which the C-index was 0.81 (95%CI, 0.73-0.89), had greater improvement than previous staging systems. The internal cross-validation and DCA also confirmed its great clinical benefits. The AS demonstrated its prognostic significance in Chinese patients, and the novel renal staging system based on AS and eGFR may provide great prognostic guidance for these patients.

Quaternary environment and climate change reconstruction from geochemical and geoarchaeological evidences of paleosols in east-central Barind, NW Bangladesh

This research focuses on analyzing paleosols, major and trace element geochemistry, organic carbon levels, and geo-archaeological aspects of Quaternary deposits investigated in two sedimentary successions located in the east-central Barind region of NW Bangladesh. Several factors that influence the paleosol development can be used to decipher the paleoenvironment and paleoclimatic conditions of the study area. Sedimentary succession of the studied sections have been grouped into two broad categories i.e., Gray unit/Newer Alluvium (NA) of Late Holocene and Red unit/Older Alluvium (OA) of Early Pleistocene age. Some geoarchaeological evidences have been found at the bottom of the gray unit and the top of the red unit in Durgadaha (DD) section indicating the existence of paleo-settlement at about ∼1300 years BP. The age is determined by the relative dating of several artifacts found at the paleo-settlement surface. Interpretation of several field characteristics and geochemical parameters i.e., clayeyness, salinization, base loss, calcification, leaching (Ba/Sr), aeolian input (Zr/Al), CIA, CIW, CIA-K, etc. revealed that the paleosols in gray unit are weakly developed; whereas, paleosols in red unit are relatively moderate, strong to very strongly developed. In the red unit, the MAP and MAT range from 1000 ± 181 mm to 1478 ± 181 mm and 9° ± 4.4°–14° ± 4.4 °C respectively. On the other hand, MAT ranges from 23.1° ± 0.6 °C to 28.3° ± 0.6 °C in gray unit paleosols. Depending on the depositional pattern and estimated MAT, five short-term climatic cycles (i.e., alternating phases of dry and wet) have been recognized in gray/newer alluvium units during the last 1300 years. The demise of paleo settlement (1300 years BP) due to the abrupt climatic change towards a dry and cooler phase where the MAT was estimated as 23.1° ± 0.6° which is at least 3 °C lower than the present. This study also revealed that the estimated MAP and MAT are more analogous to paleoclimatic records of the Asian regions.

Changes in marine sedimentation patterns in the northeastern South China Sea in the past 35,000 years

In the continental margin of the northeastern South China Sea, the sea level fluctuations since the Last Glacial Maximum have profoundly impacted the sedimentary environment. Our sub-bottom profiler data show a sedimentation process change from deposition to erosion during the Last Glacial Maximum. After the widespread erosion, the sedimentation process returned depositional throughout the Holocene, probably due to the rise of the sea level. This sedimentary process results in a widespread sedimentary unconformity in the continental slope, providing a benchmark for the end of the Last Glacial Maximum. Analyzing the sediment cores, we affirm that the change in current intensity is the primary factor controlling the sedimentary environments. The current intensities strengthened during the eustatic lowstand and weakened during the highstand periods, leading to alternating erosional and depositional processes. The widespread distribution of the erosive surface represents a regional-scale change in the sedimentary environment instead of a local event.