Male Patterns Research Articles

Career patterns and career satisfaction of women and men in Poland in years 1990-2010

ABSTRACT This study aims to identify the career patterns of Polish women (345) and men (305) and to compare their professional and life satisfaction. Career events serve as a basis for identifying three career dimensions: stability, vertical mobility and continuity. Cluster analysis revealed three female and three male career patterns. The most frequently pursued career for women and men is a conventional pattern. Women` careers is characterized by less continuity, mobility, and stability than men's. The results show that the schema of the traditional social role is more often revealed regardless of gender, while the non-traditional schema of social role is revealed in mobile careers of women. Women pursuing discontinuous careers, and men pursuing unstable careers are significantly less satisfied with career than people with a mobile career. Women are the most satisfied with a career typical of traditional male career, which includes no brake in career, job stability and promotions. Men's satisfaction with life depends on their career while women are satisfied regardless of the career pattern.

Seasonal variation in the diurnal activity pattern of Eurasian blackbirds (Turdus merula) in the forest

Camera traps are increasingly used to estimate the density of animals as well as their activity patterns. As camera traps allow monitoring of animals over long periods of time without disturbance, they are especially useful to observe changes in diurnal activity patterns over time. In ornithology, camera trapping is still in its infancy. To our knowledge, no study has yet investigated the activity pattern of a songbird over the full annual cycle. We used camera traps in the Rammert, a small mountainous forest area near Rottenburg in Southwest Germany to monitor the diurnal activity pattern of forest-dwelling Eurasian blackbirds (Turdus merula). As the activity level of animals is known to be affected by day light, we used double-anchoring transformation of day times to account for the variation in sunrise and sunset across the different seasons. By generating activity models, we investigated the pattern of blackbird activity during the four seasons of the year and compared the patterns of male and female birds, respectively. A significant difference between a unimodal activity pattern in spring and a bimodal pattern for the rest of the year was found which might be related to breeding and territorial behaviour in spring. Moreover, we observed that the activity pattern of males and females overlapped greatly but still showed some variation in the number and timing of density peaks.

Characteristics of octopus fishery in Ampana Tojo Una-Una Sea waters

Octopus fishing activity have been established in Ampana Waters and surrounding areas since the 1990s. The goal of this research is to examine the octopus fishery in Ampana waters and its surroundings. The research methodology used in this study are CPUE and Length-based assessment which includes some analysis of catch per unit effort, fishing season, size structure, weigh distribution, length-weight relationship, fishing ground, fishing gear operation and specification, and the marketing flow. CPUE ranges from 7–15 kg/units/day, with an average of 11 kg/units/day; the fishing season runs from August to November; mantle length ranges from 8–22 cmML, and the length mode is 14 cmML; male octopus length ranges from 9–19 cmML, while the female ranges from 8–22 cmML; weight ranges from 300–2500 gr, with the male dominant at 800 gr and the female dominant at 1000 gr. Growth patterns for both males and females are negative allometric. Ampana fishermen fished around Tanjung Api, while Pagimana fishermen fished north of Bunta beach. Fishing equipment used included an octopus fishing rod, hook, and arrow, as well as aggregating devices such as gara-gara and a crustacean-like octopus fishing rod. The fishermen sold their catch to the first collector, who then delivered it to octopus middlemen in Ampana, Pagimana, and Luwuk before transporting it to Makassar.

Metabolic dysfunctions following chronic oral corticosterone are modified by adolescence and sex in mice

Adolescence is a period of development in which shifts in responses to glucocorticoids is well-documented. Obesity and metabolic syndrome are substantial health issues whose rates continue to rise in both adult and adolescent populations. Though many interacting factors contribute to these dysfunctions, how these shifts in glucocorticoid responses may be related remain unknown. Using a model of oral corticosterone (CORT) exposure in male and female mice, we demonstrate differential responses during adolescence (30–58 days of age) or adulthood (70–98 day of age) in endpoints relevant to metabolic function. Our data indicate that CORT resulted in significant weight gain in adult- and adolescent-exposed females and adult-exposed males, but not adolescent-exposed males. Despite this difference, all animals treated with high levels of CORT showed significant increases in white adipose tissue, indicating a dissociation between weight gain and adiposity in adolescent-treated males. Similarly, all experimental groups showed significant increases in plasma insulin, leptin, and triglyceride levels, further suggesting potential disconnects between overt weight gain, and underlying metabolic dysregulation. Finally, we found age- and dose-dependent changes in the expression of hepatic genes important in glucocorticoid receptor and lipid regulation, which showed different patterns in males and females. Thus, altered transcriptional pathways in the liver might be contributing differentially to the similar metabolic phenotype observed among these experimental groups. We also show that despite little CORT-induced changes in the hypothalamic levels of orexin-A and NPY, we found that food and fluid intake were elevated in adolescent-treated males and females. These data indicate chronic exposure to elevated glucocorticoid levels results in metabolic dysfunction in both males and females, which can be further modulated by developmental stage.

Variation of insulin-related peptides accompanying the differentiation of Aphis gossypii biotypes and their expression profiles.

Insulin signaling plays a critical role in regulating various aspects of insect biology, including development, reproduction, and the formation of wing polyphenism. This leads to differentiation among insect populations at different levels. The insulin family exhibits functional variation, resulting in diverse functional pathways. Aphis gossypii Glover, commonly known as the cotton-melon aphid, is a highly adaptable aphid species that has evolved into multiple biotypes. To understand the genetic structure of the insulin family and its evolutionary diversification and expression patterns in A. gossypii, we conducted studies using genome annotation files and RNA-sequencing data. Consequently, we identified 11 insulin receptor protein (IRP) genes in the genomes of the examined biotypes. Among these, eight AgosIRPs were dispersed across the X chromosome, while two were found in tandem on the A1 chromosome. Notably, AgosIRP2 exhibited alternative splicing, resulting in the formation of two isoforms. The AgosIRP genes displayed a high degree of conservation between Hap1 and Hap3, although some variations were observed between their genomes. For instance, a transposon was present in the coding regions of AgosIRP3 and AgosIRP9 in the Hap3 genome but not in the Hap1 genome. RNA-sequencing data revealed that four AgosIRPs were expressed ubiquitously across different morphs of A. gossypii, while others showed specific expression patterns in adult gynopara and adult males. Furthermore, the expression levels of most AgosIRPs decreased upon treatment with the pesticide acetamiprid. These findings demonstrate the evolutionary diversification of AgosIRPs between the genomes of the two biotypes and provide insights into their expression profiles across different morphs, developmental stages, and biotypes. Overall, this study contributes valuable information for investigating aphid genome evolution and the functions of insulin receptor proteins.

LANGUAGE AND GENDER – MALE AND FEMALE LANGUAGE PATTERNS IN THE GREEK SERIES MHN APXIZEIΣ THN MOYPMOYPA

Television is a dominant medium that can influence one’s attitude and social ideologies formation since it reflects sociolinguistic reality and maintains language ideologies and existing social stereotypes. For that reason, we can mention so-called female and male language patterns and their main characteristics, which are to a great extent associated with social power, i.e. the role that an individual has in his social community or community of practice. This paper aims to examine whether the Greek media and more specifically, the Greek TV series promote so-called male and female language patterns, as well as social stereotypes directed towards women. In this paper, we analysed the linguistic behaviour of the protagonists of the Greek series Mην αρχίζεις την μουρμούρα. Qualitative non-experimental research based on the analysis of linguistic behaviour at the level of the characters‘ lexical choice, Vaso and Charis showed that their choice of language patterns is proportional to their social or professional status. In addition, we noticed the presence of certain social stereotypes, while it is evident that the use of stereotypical-female language patterns is associated with a hierarchically lower social position.

Stability and change in male fertility patterns by cognitive ability across 32 birth cohorts.

The relationship between cognitive ability (CA) and childbearing remains unsettled. Using Norwegian administrative registers with population coverage, we study how male lifetime fertility patterns differ across cognitive score groups, and how these changed across the 1950-1981 birth cohorts, covering a period characterized by rapid social and economic change. The analyses reveal systematic differences in fertility and fertility timing across CA groups, with high scoring males having delayed but ultimately higher fertility than lower scoring males. This pattern remains stable over time despite strong trends towards delayed and reduced fertility. The overall positive relationship between CA and fertility is primarily driven by high rates of childlessness in the lowest scoring group, with low-scoring males showing higher rates of parity progression at higher parities.

First report of DNA detection and sex determination in leaked fluid after needle collapse of large equine blastocysts

The current method for sex determination of equine blastocysts involves aspiration of blastocoel fluid followed by PCR, which can be challenging due to the need for specialized equipment and often requires the embryo to be shipped to a separate lab for analysis. In this study, we investigated a new method for sex determination using leakage from manual collapse prior to vitrification, a technique which previously resulted in a pregnancy in our laboratory (1 of 3, unpublished results). We hypothesized that leaked blastocele fluid into the surrounding medium could provide sufficient DNA for amplification and sex determination. From a total of 14 embryos (900 to 1200 μm diameter), 4 were collapsed by micromanipulator-assisted aspiration (Micro group), and 10 were collapsed by manual puncture (Needle group) using a 32-G, penta-point needle (BD Nano, Canada). Additional blastocoel leakage (up to 80% of original volume) was obtained by forced pipetting using a 275 μm flexipet. All manipulations were conducted in TALP without serum. Samples were snap-frozen (30 μl) and stored at -80°C. DNA amplification was performed by multiplex PCR targeted to sex chromosome-linked zinc finger protein genes (ZFx/ZFy) for both sex DNA detection and the Y-encoded testis-specific protein (TSPY) for Y chromosome detection. The primer sequences were ZFx/y-F 5’-ATAATCACATGGAGAGCCACAAGCT-3’, ZFx/y-R 5’-GCACTTCTTTGGTATCTGAGAAAGT–3’, and TSPY-F 5′-GAAGTCAGGCACACCAGTGA-3′, TSPY-R 5′-TAAGGCTGCAGTTGTCATGC-3′, with 445bp and 280bp products, respectively. The reaction was carried out in a 25 μl final volume with 4 μl of PROMEGA Green GOTaq 5X Buffer, 0.2 μM primers, 1 mM MgCl2, 5 μl DNA sample, and 1.25 IU Taq DNA polymerase. The cycle conditions were 4 min 94°C, 40 cycles of 30 sec 94°C, 30 sec 58°C, and 20 sec 72°C followed by final elongation of 1 min 72°C. Equine male and female DNA was purified from whole blood (PROMEGA WizardTM Genomic DNA purification kit) and used as positive controls; female and male band patterns were used as reference. Our results showed that sex was confirmed in 4/4 (100%; 3 male and 1 female) of embryos biopsied by micromanipulation (Micro group) and in 5/10 (50%; 1 male and 4 female) of embryos collapsed by manual needle puncture (Needle group). Although the proportion of sex determination in the manually collapsed group is still low, it could be attributed to sample dilution effects. This report provides proof of concept of DNA detection and sex determination in the medium surrounding manually collapsed embryos without blastocoel aspiration. Our findings suggest that this approach to embryo biopsy for sex determination is promising, but more studies are needed to confirm its effectiveness.

Female age and reproductive stage influence copulation patterns in mountain gorillas’ variable mating system

Variation in mating systems and in individuals’ copulation patterns can have important consequences for the genetic structure of a population and ultimately its evolution. Whilst most gorilla sub-species form single-male, polygynous reproductive groups, mountain gorillas have both single-male and multimale reproductive groups. Considerable research has investigated the mating patterns of males in these multimale groups, but comparatively little is known about females. We investigated the copulation patterns of 71 female mountain gorillas over 13 years. We found that most sexually active, fecundable females in multimale groups had multiple mating partners. Females’ copulation patterns varied based on their reproductive stage, mating most often and with the greatest number of partners when they were pregnant, and least often when they had young dependent offspring. Females copulated throughout pregnancy, with pregnant females copulating more frequently in single-male groups than in multimale groups. Mating also varied with age, with older females mating less often and with fewer partners. Our findings confirm that when females transfer between single-male and multimale groups, they predominantly switch from copulating with one male to copulating with multiple males, and that females are also flexibly adjusting their copulation patterns across their lifetimes. This highlights the considerable variability of the mountain gorillas mating system, particularly from the female perspective, and the importance of accounting for within-species variation in copulation patterns.Significance statementPatterns of mating can vary both between individuals of the same species and within individuals across their lives. These patterns influence the genetic structure of a population. By examining copulation patterns in female mountain gorillas, we demonstrate that whilst females in single-male groups have only one mating partner, females in multi-male groups predominantly have multiple partners across a year. They also alter their copulation patterns across their lifetimes, copulating less often and with fewer partners as they age, and copulating most when pregnant. Our results highlight the flexibility with which female mountain gorillas can adjust their copulation patterns across their lifetimes, as the costs and benefits of copulation shift.

Length-weight relationship and growth of Labeo bata

This study was executed to elucidate the length-weight relationship (LWR) yet the annual condition of the Labeo bata (Hamilton, 1822), an immensely important fishery of Bangladesh. Overall, 447 specimens employed during this study were collected by using traditional fishing gear (cast net). Total length (TL) of each individual was measured closest to 0.1 cm and whole body-weight (BW) nearest to 0.01 g accuracy, respectively. The values of ‘b’ indicated negative allometric growth patterns for males (b= 2.913) whilst an isometric value for females (b= 3.038) and both sexes (b= 2.989), respectively. Furthermore, the Fulton condition factor value was highest for males (0.98) over females (0.97). All of these outcomes will bring advanced scientific knowledge and will be helpful for sustainable management of this least concern fish species (Labeo bata) in the river ecosystem of Bangladesh.

Overview of Avian Sex Reversal.

Sex determination and differentiation are processes by which a bipotential gonad adopts either a testicular or ovarian cell fate, and secondary sexual characteristics adopt either male or female developmental patterns. In birds, although genetic factors control the sex determination program, sex differentiation is sensitive to hormones, which can induce sex reversal when disturbed. Although these sex-reversed birds can form phenotypes opposite to their genotypes, none can experience complete sex reversal or produce offspring under natural conditions. Promising evidence indicates that the incomplete sex reversal is associated with cell autonomous sex identity (CASI) of avian cells, which is controlled by genetic factors. However, studies cannot clearly describe the regulatory mechanism of avian CASI and sex development at present, and these factors require further exploration. In spite of this, the abundant findings of avian sex research have provided theoretical bases for the progress of gender control technologies, which are being improved through interdisciplinary co-operation and will ultimately be employed in poultry production. In this review, we provide an overview of avian sex determination and differentiation and comprehensively summarize the research progress on sex reversal in birds, especially chickens. Importantly, we describe key issues faced by applying gender control systems in poultry production and chronologically summarize the development of avian sex control methods. In conclusion, this review provides unique perspectives for avian sex studies and helps scientists develop more advanced systems for sex regulation in birds.

Song structure of male Northern House Wrens and patterns of song production and delivery across the nesting cycle

Song structure of male Northern House Wrens and patterns of song production and delivery across the nesting cycle

Sex-specific effects of voluntary wheel running on behavior and the gut microbiota-immune-brain axis in mice

Physical exercise has been positioned as a promising strategy to prevent and/or alleviate anxiety and depression, but the biological processes associated with its effects on mental health have yet to be entirely determined. Although the prevalence of depression and anxiety in women is about twice that of men, very few studies have examined whether physical exercise could affect mental health differently according to sex. This study examined, in singly-housed mice, the sex-specific effects of voluntary exercise on depressive- and anxiety-like behaviors as well as on different markers along the gut microbiota-immune-brain axis. Male and female C57BL/6N mice had voluntary access to running wheels in their home-cages for 24 days or were left undisturbed in identical home-cages without running wheels. Behaviors were then examined in the open field, splash, elevated plus maze, and tail suspension tests. Gene expression of pro-inflammatory cytokines, microglia activation-related genes, and tight junction proteins was determined in the jejunum and the hippocampus, while microbiota composition and predicted function were verified in cecum contents. Voluntary exercise reduced anxiety-like behaviors and altered grooming patterns in males exclusively. Although the exercise intervention resulted in changes to brain inflammatory activity and to cecal microbiota composition and inferred function in both sexes, reductions in the jejunal expression of pro-inflammatory markers were observed in females only. These findings support the view that voluntary exercise, even when performed during a short period, is beneficial for mental and intestinal health and that its sex-specific effects on behavior could be, at least in part, related to some components of the gut microbiota-immune-brain axis.

Female preference for rare males is maintained by indirect selection in Trinidadian guppies

When females prefer mates with rare phenotypes, sexual selection can maintain rather than deplete genetic variation. However, there is no consensus on why this widespread and frequently observed preference might evolve and persist. We examine the fitness consequences of female preference for rare male color patterns in a natural population of Trinidadian guppies, using a pedigree that spans 10 generations. We demonstrate (i) a rare male reproductive advantage, (ii) that females that mate with rare males gain an indirect fitness advantage through the mating success of their sons, and (iii) the fitness benefit that females accrue through their "sexy sons" evaporates for their grandsons as their phenotype becomes common. Counter to prevailing theory, we show that female preference can be maintained through indirect selection.

Emergence of Sex Differences in U.S. Adolescent Substance Use

Background: Adolescence is a common time for experimentation with substance use and the emergence of sex differences in substance use patterns. Although similar in early adolescence, male and female substance use patterns historically diverge by young adulthood, with males using more substances than females. We aim to add to current literature by utilizing a nationally representative sample, assessing a broad range of substances used, and focusing on a sentinel period during which sex differences emerge. We hypothesized that certain sex-specific substance use patterns emerge in adolescence. Methods: Data are from the 2019 Youth Risk Behavior Survey (n = 13,677), a nationally representative sample of high school students. Weighted logistic analyses of covariance adjusting for race/ethnicity evaluated males’ and females’ substance use (14 outcomes) by age category. Results: Among all adolescents, more males reported illicit substance use and cigarette smoking than females, whereas more females reported prescription opioid misuse, synthetic cannabis use, recent alcohol use, and binge drinking. Divergence between male and female use usually occurred at 18+ years. Odds of using most illicit substances were significantly greater among males than females at age 18+ years (aORs 1.7–4.47). Among 18+ year-olds, males and females did not differ in electronic vapor product use, alcohol use, binge drinking, cannabis use, synthetic cannabis use, cigarette smoking, or prescription opioid misuse. Conclusions: Sex differences in adolescent use of most but not all substances emerge by age 18+ years. Sex-specific patterns of adolescent substance use may inform specific prevention efforts and identify peak ages for intervention.

Reproductive Biology of Male European Hake (Merluccius merluccius) in Central Mediterranean Sea: An Overview from Macroscopic to Molecular Investigation.

The improvement of scientific knowledge about overexploited fishery resources allow us to provide scientific advice for their management and stock protection. By using a multidisciplinary approach, the aim of the study was to characterize, for the first time in the Central Mediterranean Sea (GSA 17), the reproductive biology of males of M. merluccius, currently highly exploited. A multi-year sampling from January 2017 to December 2019 was performed to exhaustively evaluate the sex ratio of the stock, while the 2018 annual sampling was selected to investigate the reproductive pattern of males. Individuals in spawning conditions were found every month, proving that M. merluccius is an asynchronous species, reproducing all year round, with a seasonal reproductive peak in spring/summer, as indicated by GSI. Five gonadal development stages were defined to fully describe the reproductive cycle of males. The macroscopic and histological L50, respectively 18.6 cm and 15.4 cm, were both below the Minimum Conservation Reference Size (MCRS). According to the mRNA levels, fsh and lh played a significant role during spermiation, whereas the gnrhr2a was involved at the beginning of sexual maturity. In the testis, fshr and lhr reached maximum expression levels before the spermiation. The hormonal stimuli of 11-ketotestosterone and its receptor were significantly higher when the specimen was in reproductive activity.

Exploring the association of physical activity with the plasma and urine metabolome in adolescents and young adults

BackgroundRegular physical activity elicits many health benefits. However, the underlying molecular mechanisms through which physical activity influences overall health are less understood. Untargeted metabolomics enables system-wide mapping of molecular perturbations which may lend insights into physiological responses to regular physical activity. In this study, we investigated the associations of habitual physical activity with plasma and urine metabolome in adolescents and young adults.MethodsThis cross-sectional study included participants from the DONALD (DOrtmund Nutritional and Anthropometric Longitudinally Designed) study with plasma samples n = 365 (median age: 18.4 (18.1, 25.0) years, 58% females) and 24 h urine samples n = 215 (median age: 18.1 (17.1, 18.2) years, 51% females). Habitual physical activity was assessed using a validated Adolescent Physical Activity Recall Questionnaire. Plasma and urine metabolite concentrations were determined using ultra-high-performance liquid chromatography-tandem mass spectroscopy (UPLC-MS/MS) methods. In a sex-stratified analysis, we conducted principal component analysis (PCA) to reduce the dimensionality of metabolite data and to create metabolite patterns. Multivariable linear regression models were then applied to assess the associations between self-reported physical activity (metabolic equivalent of task (MET)-hours per week) with single metabolites and metabolite patterns, adjusted for potential confounders and controlling the false discovery rate (FDR) at 5% for each set of regressions.ResultsHabitual physical activity was positively associated with the “lipid, amino acids and xenometabolite” pattern in the plasma samples of male participants only (β = 1.02; 95% CI: 1.01, 1.04, p = 0.001, adjusted p = 0.042). In both sexes, no association of physical activity with single metabolites in plasma and urine and metabolite patterns in urine was found (all adjusted p > 0.05).ConclusionsOur explorative study suggests that habitual physical activity is associated with alterations of a group of metabolites reflected in the plasma metabolite pattern in males. These perturbations may lend insights into some of underlying mechanisms that modulate effects of physical activity.

A deep transcriptome meta-analysis reveals sex differences in multiple sclerosis

BackgroundMultiple sclerosis (MS), a chronic auto-immune, inflammatory, and degenerative disease of the central nervous system, affects both males and females; however, females suffer from a higher risk of developing MS (2–3:1 ratio relative to males). The precise sex-based factors influencing risk of MS are currently unknown. Here, we explore the role of sex in MS to identify molecular mechanisms underlying observed MS sex differences that may guide novel therapeutic approaches tailored for males or females. MethodsWe performed a rigorous and systematic review of genome-wide transcriptome studies of MS that included patient sex data in the Gene Expression Omnibus and ArrayExpress databases following PRISMA statement guidelines. For each selected study, we analyzed differential gene expression to explore the impact of the disease in females (IDF), in males (IDM) and our main goal: the sex differential impact of the disease (SDID). Then, for each scenario (IDF, IDM and SDID) we performed 2 meta-analyses in the main tissues involved in the disease (brain and blood). Finally, we performed a gene set analysis in brain tissue, in which a higher number of genes were dysregulated, to characterize sex differences in biological pathways. ResultsAfter screening 122 publications, the systematic review provided a selection of 9 studies (5 in blood and 4 in brain tissue) with a total of 474 samples (189 females with MS and 109 control females; 82 males with MS and 94 control males). Blood and brain tissue meta-analyses identified, respectively, 1 (KIR2DL3) and 13 (ARL17B, CECR7, CEP78, IFFO2, LOC401127, NUDT18, RNF10, SLC17A5, STMP1, TRAF3IP2-AS1, UBXN2B, ZNF117, ZNF488) MS-associated genes that differed between males and females (SDID comparison). Functional analyses in the brain revealed different altered immune patterns in females and males (IDF and IDM comparisons). The pro-inflammatory environment and innate immune responses related to myeloid lineage appear to be more affected in females, while adaptive responses associated with the lymphocyte lineage in males. Additionally, females with MS displayed alterations in mitochondrial respiratory chain complexes, purine, and glutamate metabolism, while MS males displayed alterations in stress response to metal ion, amine, and amino acid transport. ConclusionWe found transcriptomic and functional differences between MS males and MS females (especially in the immune system), which may support the development of new sex-based research of this disease. Our study highlights the importance of understanding the role of biological sex in MS to guide a more personalized medicine.

Achieving Gender Equality through Paid and Unpaid Work: An Exploration of Mothers’ Perspectives on Work

The prevailing understanding of work as paid work is reflected in political efforts to achieve gender equality, which include emphasising that women, like men, should increasingly pursue paid work. This exploratory research aims to question whether this idea to align female with male life patterns is conducive to gender equality and to promote new insights based on mothers’ experiences. Our analysis is based on guided interviews with eight Swiss mothers in part-time employment who have at least one child aged three or older, and a working partner in the same household. The interviews show that these mothers do not share the expectation that all mothers should take on the main responsibility for domestic and care work, nor the expectation that all women should be doing full-time paid work. They would like to see greater acceptance and appreciation of different forms of work. This research concludes that gender justice can be understood as a freedom of choice that includes both the right to be doing paid work and the right to have time for domestic and care work—for men and women. Gender equality efforts do not have to be restricted to one form of work, but can leave room for different types of work and the appreciation of them.

Semiochemical Release and Ontogenetic Changes in a Primary Scent Gland of Podisus maculiventris.

The spined shoulder bug, Podisus maculiventris, is a generalist predator studied for its biocontrol potential. Despite our growing understanding of gland development, the conditions that elicit releases are largely unknown. To determine if male age or gland development affects the chemical composition and release behavior, we dissected adult male bugs and profiled the chemical composition of the male DAG 1, 7, and 14 d post-eclosion. To determine if gland development is related to sexual maturity, we counted the number of sperm present in the seminal vesicles at the same time points. Finally, we measured the diurnal release patterns of different aged males and in various male-female combinations. We observed that newly eclosed adults have under-developed glands and male seminal vesicles contained few sperm. One week post-eclosion the DAG contained previously reported semiochemical compounds and males contained many sperm. Mirroring the trend in reproductive maturation and gland development, the number of semiochemical releases increased with age and the majority of releases followed a scotophase pattern unaffected by sexual composition. These findings link male age to 1) dorsal abdominal gland development 2) release behavior and 3) sexual maturity, which will help our understanding of when these olfactory cues are present for other organisms, like prey, to perceive. Given the results, releasing adults that are at least 1week post eclosion will maximize the non-consumptive effects of this biocontrol agent.