Background: The conjunctivitis, known generally as pink eyes, is the inflammation of the inner eyelids, as well as the entire surface of the eye´s outermost part, which makes it get a reddish-pink look. The symptoms may involve pain, burning and itching, most often when an allergic cause is implicated. Method: The present work considered 306 scenarios of human blepharitis, cultured swabs were plated on MacConkey agar and Blood agar used streak technique and incubated at 37°C in a 24-hour experiment. Bacterial isolates were pointed based on their appearance, gram-staining, direct microscopy and biochemical tests comprising of catalase, oxidase, mannitol fermentation and hemolysis. Enterosystem 18 R system and the Vitek-2 compact system were used for the confirmation of bacterial species. Microplate method was used to test for biofilm formation of the bacterial isolates. Results: Antibiotic resistance test of isolates, however, reflected relatively high resistance among them, with Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumoniae, Moraxella catarrhalis and Klebsiellapneumoniae all being 100% resistant to amoxicillin. Klebsiella pneumonia and E. coli isolates showed a 90% resistance to moxifloxacin, and to ciprofloxacin by Staphylococcus aureus isolates. Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, and Streptococcus pneumoniae exhibited significant biofilm production, whereas the other isolated species showed lower biofilm-forming tendencies.