Remote patient monitoring using mobile devices can be a cost-effective, time-saving solution limiting patient mobility. Direct observation of therapy (DOT) of tuberculosis patients in Malaysia usually involves traveling to the healthcare facility. Due to the contagious nature of the disease, who comes into contact with patients is at risk of developing it. Additionally, DOT is time–consuming and costly for patients. A smartphone application for remote tuberculosis patient monitoring called video directly observed therapy (VDOT) can be a cost-effective and safer way of patient monitoring. However, before implementing this technology, the user readiness assessment helps policymakers' decision-making. This pre-implementation phase study aims to ascertain users' intent to utilize the VDOT app. The current study examines users' readiness using a modified Unified Theory of Acceptance and Use of Technology (UTAUT). The cross-sectional survey took place in Selangor and the federal territory of Kuala Lumpur, Malaysia. A total of 220 questionnaires were delivered to prospective users, including patients and family members. 68% percent of the questioners were deemed usable and were evaluated using SPSS. The study's findings indicate that users intend to adopt the VDOT app for tuberculosis remote monitoring. Performance expectancy, social influence, facilitating conditions, and trust influence users' behavioral intention to adopt the VDOT app in Malaysia.