Patients In Isfahan Research Articles

An analysis and survey of interleukin-10 receptor mutation in inflammatory bowel disease (IBD) in the first Iranian IBD cohort

Abstract Background Early-onset inflammatory bowel disease (IBD) is classified into Crohn’s disease (CD), ulcerative colitis (UC) and unclassified disorders, which has a chronic, relapsing course and can result in substantial long-term morbidity. IBD is a multifactorial disorder with genetic susceptibility, immunological predisposition and environmental triggers. The objective of this study was to generally determine the prevalence of IL10R mutation in IBD patients in Isfahan, Iran. We performed sequencing of all exons in IL10RA and IL10RB in a cohort of IBD patients and healthy controls. Methods Total DNA contents of 76 patients and 50 healthy controls were extracted from whole blood and polymerase chain reaction (PCR) amplifications and sequencing of whole exons in IL10R were performed. Results Overall, we determined 13 single nucleotide polymorphisms (SNPs) in all IL10R genes. Of them, rs3135932 and rs2229113 of the IL10RA1 gene, in exons 4 and 7, respectively, were significantly associated with IBD occurrence in patients. Conclusions Our results also confirmed that early-onset IBD could be attributed to a synergistic effect of several variant alleles of the genes encoding IL10 receptors. These variants, alone, could only give rise to a sub-clinical manifestation of IBD.

An Investigation of the Effects of the Acceptance and Commitment Therapy in Groups on the Cognitive Strategies of Emotion Regulation and Self-Control in Coronary Heart Disease Patients.

Coronary heart diseases are amongst the most common and severe diseases; also, the prevalence and emergence of these diseases are largely affected by psychological and social factors. The present study was conducted in order to investigate the effects of acceptance and commitment therapy in groups on the cognitive strategies of emotion regulation and self-control in patients with cardiovascular diseases.The present work is a quasi-experimental research with a pretest-posttest design and a control group.The statistical population of this study included all patients with coronary heart disease aged 35-55 years who referred to hospitals, health centers, and cardiovascular clinics of Isfahan in the spring of 2018. Among these patients, 30 patients were selected based on the inclusion criteria of the study using a convenience sampling method. They were then divided randomly into two experimental and control groups, with 15 participants in each group. The patients in the experimental group participated in eight 60-minute sessions of the Hayes’ Acceptance and Commitment Therapy (ACT) (2002). However, no intervention was applied to the control group. The participants responded to the research questionnaires in two phases. The questionnaires included the Garnefski’s Cognitive Emotion Regulation Questionnaire (CERQ) (2001) and the Tangji’s self-control questionnaire (2004). The research data were analyzed using covariance analysis in SPSS 24.The results of the covariance analysis indicated that the group-based acceptance and commitment therapy exerted a significant effect on the total score of the two strategies, i.e., the positive strategy of the cognitive regulation of emotions and the negative strategy of the cognitive regulation of emotions (p<0.05). However, no significant difference was observed in terms of self-control (p> 0.05).The findings of the present study showed that the treatment intervention resulted in increased use of acceptance strategies, positive re-focus, and re-focus, as well as reduced use of rumination and other-blaming strategies in cardiovascular patients in Isfahan.

Evaluation of metastasis and 5-year survival in oral squamous cell carcinoma patients in Isfahan (2001–2015)

Frequency analysis of metastasis in patients with oral squamous cell carcinoma (OSCC) helps to partly anticipate the upcoming chance of metastasis in the patients following primary diagnosis. This study was conducted to determine the evaluation of metastasis and 5-year survival in OSCC. This was a retrospective, descriptive study. A total of 96 OSCC patients were studied. Demographic data and clinical view of lesion as well as histopathological grade of patients were recorded in previously prepared forms of pathology centers. These findings were then evaluated in relation to the 5-year survival. The obtained data were fed into SPSS and analyzed by Chi-square test, t-test, one-way ANOVA, and Scheffé test. A significant level of α = 0.05 was used. The 5-year survival of patients was 41.7%. From among the patients, 44% were found to have local recurrence and 36% were reported to have distant metastasis. There was no significant association between the 5-year survival rate and primary grade (P = 0.105). However, it revealed a significant relationship between the 5-year survival rate and distant metastasis (P = 0. 001). This study showed that the 5-year survival is dependent on the primary site of involvement, local recurrence, regional and distant metastasis, and mean age. However, gender (P = 0.89), grade (P = 0.105), and stage (P = 0.242) have no effect on the 5-year survival of patients.

Genetic Variation in Intergenic and Exonic miRNA Sequence and Risk of Multiple Sclerosis in the Isfahan Patients

MicroRNAs (miRNAs), have been documented to perform a key role in the pathogenesis of multiple sclerosis (MS), a chronic inflammatory and autoimmune disease. Recent studies have shown that single nucleotide polymorphism in the sequence of the miRNA may change their production and expression which can lead to miRNA dysfunction and pathogenicity. Some studies have reported the relationship between miRNA polymorphism and the increased risk of autoimmune disease. This study was conducted to investigate the association between mir155 rs767649, mir196a2 rs11614913 and mir23a rs3745453 polymorphism and the risk of multiple sclerosis in the Iranian MS patients in Isfahan. A population of 80 patients and the same number control were selected. After DNA extraction, genotyping was performed through tetra amplification refractory mutation system-PCR method (T ARMS PCR). The frequencies of TT, TC and CC genotypes of mir23a were 46, 35 and 20% in MS patients and 42, 14 and 24 in healthy subjects respectively. These results showed that individuals carrying the genotypes of rs3745453 TC had a 2.3-fold increased risk of MS (OR=2.3, p=0.048). There was no significant difference between genotypes and allele frequency of mir155 and mir196a2 in patients and healthy controls (p>0.05). Our findings specified that CT heterozygosity in mir23a gene significantly related with risk of MS. Unlike mir155 and mir196a2, mir23a rs3745453 may have contributed to the etiology of MS in Isfahan patients. However, extensive studies are required to gain more reliable and authentic results.

Social Support and Depression in Iranian Cancer Patients: the Role of Demographic Variables.

Introduction: Depression is the most frequent mental disorder in cancer patients, which is, unfortunately, less noticeable. Despite extensive researches, we only have scant knowledge about the influence of social support on depression in cancer patients. The aim of this study is to examine the relationship between perceived social support and depression and demographic variables on cancer patients. Methods: This is a descriptive correlational study on two hundred cancer patients in Isfahan, Iran. The study subjects were selected through convenient sampling method and data were collected using questionnaires (demographic questionnaire, Multidimensional Scale of Perceived Social Support (MSPSS) and Beck Depression Scale (BDS). Data were analyzed by SPSS software (Frequency distribution, Chi-Square and Spearman correlation). Results: The findings showed that social support and depression in cancer patients are significantly correlated with each other (r = - 0.785, P <0.001). Furthermore, the findings indicated that there are statistically significant correlations between degrees of education with depression and also between the numbers of children with social support and depression in cancer patients. For all analyses, was considered significant. Conclusion: In conclusion, we suggest that interventions to alleviate depression in cancer patients may be designed with consideration of demographic characteristics especially the level of education and the number of supporters of the patient to increase the quality of life in cancer patients and minimize the negative consequences of cancer diagnosis.

Investigation of Class I, II, and III Integrons Among Acinetobacter Baumannii Isolates from Hospitalized Patients in Isfahan, Iran.

This study aimed to determine the prevalence of class I, II, and III integrons among clinical Acinetobacter baumannii isolates collected from hospitalized patients. This cross-sectional study was conducted at two teaching hospitals in Isfahan, Iran, from October 2015 to October 2016. A total of 147 non-duplicate A. baumannii isolates were collected from clinical specimens and identified as A. baumannii using standard microbiological methods and confirmed by genotyping. Antimicrobial susceptibility was determined using disc diffusion method, and the presence of integron genes was performed using the polymerase chain reaction. Out of 147 confirmed A. baumannii isolates, 97.3% of isolates were extensive drug-resistant (XDR) and 2.7% were multidrug-resistant (MDR). Class I and II integrons were detected in 63.9% and 78.2% of the A. baumannii, respectively. Class III integron was not detected in any of the isolates. Our results show a high prevalence of classes I and II integrons which may play a key role in the acquisition of MDR and XDR phenotype among A. baumannii isolates in our region. Therefore, use of appropriate infection control in clinical settings and implementation of treatment strategies is necessary for our hospitals.

Evaluation of Biofilm Formation and Frequency of Multidrug-resistant and Extended Drug-resistant Strain in Pseudomonas aeruginosa Isolated from Burn Patients in Isfahan.

Background:Pseudomonas aeruginosa is a biofilm-forming bacterium which can result in serious health problems, particularly in burn patients. Biofilm has been assumed to protect the bacteria from environmental fluctuations such as antimicrobial agent. Mucoid strains generate extensive levels of the alginate exopolysaccharide, which is an important factor of its biofilm.Materials and Methods:Totally, 100 isolates of P. aeruginosa has been gathered from wound infections of burn patients. Polymerase chain reaction of exoA gene has been carried out to confirm the bacteriologic identification of isolates. The biofilm-forming capacity has been specified by capsule staining and microtiter plate test as qualitative and quantitative determination, respectively. Antimicrobial susceptibility of the isolates has been specified by disk diffusion method.Results:All the isolates carried the exoA gene. The antibiotic resistance was imipenem (90%); levofloxacin (93%); aztreonam (87%); piperacillin-tazobactam (85%); tobramycin (92%); polymyxin b (PB) (2%); and ceftazidime (CAZ) (32%). Totally, multidrug-resistant (MDR) and extended drug-resistant (XDR) isolates were 19% and 75%, respectively. Fortunately, pan drug-resistant (PDR) strain has not been observed. The assessment of biofilm formation has shown that 7% of the isolates were nonbiofilm (N), weak (W) 67%, moderate (M) 22%, and strong (S) 4%.Conclusions:As a result, the findings of this survey indicated that PB and CAZ were the most effective antibiotics against P. aeruginosa, which of course indicate a serious problem about the emergence of the PDR strains. There was no relationship between the patterns of biofilm production and antibiotic susceptibility, but high frequency of MDR/XDR and biofilm producer strains has been detected.

Predictive Factors of Treatment Outcomes for Hospital Care in Children with Acute Methadone Poisoning.

Objective:The trend of methadone toxicity in children and adolescents seems to be increasing in Iran since it is used as a legal measure of the treatment for opioids addiction in methadone maintenance therapy clinics. In the present study, we describe the clinical and demographical characteristics of acute methadone toxicity in a cohort of pediatric poisoned patients in Isfahan, Iran and discussed the predictive factors for their treatment outcomes.Methods:In this 4-year cross-sectional study which was performed from 2013 to 2016 in a referral university hospital (Isfahan, Iran), medical records of the demographic and admission time clinical characteristics of all in-patients aged <18 years with acute methadone poisoning were abstracted and analyzed. According to the outcomes of hospital care and treatment, patients were divided as survived without medical complication and patients survived with at least one medical complication or death.Findings:A total number of 157 (79 male) children and adolescents with a mean age of 105.4 ± 6.1 months were hospitalized and included in the study. A total of 145 (92.4%) patients survived and discharged from the hospital without any medical complication. Pupil size, respiratory rate, and level of consciousness were predictive factors for the outcome of death or medical complications.Conclusion:It seems that methadone poisoning in children and adolescents is more commonly accidental in school-aged boys (6–12 years old) and it occurs mostly with the syrup dosage form, especially when one of the parents or people who live with the child has an addiction history and if the patients' house located in lower socioeconomic class area of Isfahan city (Iran).

Epidemiology and Clinical Features of Guillain-Barre Syndrome in Isfahan, Iran.

Background:Guillain-Barre syndrome (GBS) is an immune-mediated peripheral neuropathy. We compared clinical, laboratory characteristics, and disease course of GBS subtypes in a large group of Iranian patients in Isfahan.Materials and Methods:We collected data from patients who were admitted to Alzahra referral university Hospital, Isfahan, Iran with a diagnosis of GBS. In this population-based cross-sectional research, characteristic of 388 cases with GBS between 2010 and 2015 were studied.Results:The current study recruited 388 patients with GBS including 241 males (62.1%) and 147 females (37.9%) with a mean age of 42.78 ± 21.34. Patients with polyradiculopathy had the highest mean age of 55.12 ± 20.59 years, whereas the least age was seen in acute motor axonal neuropathy (AMAN) with the mean of 36.30 ± 18.71 years. The frequency of GBS witnessed the highest frequency in spring with 113 cases (29.1%) and winter with 101 cases (26%). Patients' electrodiagnostic findings indicated that the highest frequency pertained to AMSAN with 93 cases (24%), whereas the least frequent diagnosis was acute Polyradiculopathy with 8 cases (2.1%). Most of the patients did not have any infections (53.6%) and among patients with infections, AMSAN had the highest frequency (22.9%) and finally, patients with AMSAN and AMAN had a higher length of stay.Conclusion:The study demonstrated incidence, sex distribution, preceding infection, and surgery similar to previous studies. However, our data differs from a study in Tehran that showed acute inflammatory demyelinating polyradiculoneuropathy is more prevalent than other types and we found a seasonal preponderance in cold months, particularly in axonal types.

Relationship between self-care behaviors and health literacy in hypertensive patients in Isfahan

Introduction: Adherence to self-care behaviors among hypertensive patients is a major factor in hypertension management. Moreover, health literacy has a considerable role in controlling this condition. Hence, this study aimed to determine the self-care behaviors and their relationship to health literacy in patients with hypertension in Isfahan.

Frequency of Human Papilloma Virus Genotypes in Patient Samples From Isfahan (Iran) Using Polymerase Chain Reaction Reverse Dot Blot Method

Background: Human papilloma virus (HPV) is the most common sexually transmitted viral infection worldwide. On the basis of HPV clinical associations, this disease is divided to 2 types; high risk and low risk. high risk HPV (HR HPV) is the causative agent of cervical intraepithelial Neoplasia (CIN) and cervical cancer. low risk HPV (LR HPV) is the causative agent of diseases, such as anogenital warts and oral papillomas. Polymerase chain reaction (PCR) is one of the molecular diagnostic methods for detection of HPV DNA that has many advantages. Objectives: This study aimed at exploring the frequency of HPV genotypes using polymerase chain reaction reverse dot blot (PCR RDB) method on cervix and wart samples obtained from patients in Isfahan, Iran. Methods: The population included 111 females and 7 males. These samples were collected during 2011 and 2013. DNA was extracted from these samples and PCR was performed using reverse dot blot (RDB) method to detect HPV types. Results: With this molecular diagnostic method, different HPV types were detected in 45 samples out of 118. In 18 samples, co-infection with different HPV types was detected. Data analysis exhibited a significant correlation between age and HR HPV infection (P value 0.05). In this study, among all HPV types, HPV 16 was detected more frequently than the other HPV types. Conclusions: The PCR-RDB method has the ability to diagnose HPV infection by screening Pap smear samples and to determine the risk factor of detected HPV genotypes. This study showed that HPV16 was 1 of the most common agents in Iran, similar to other countries.

Effects of the self-management program on the quality of life among kidney-transplant patients in Isfahan's Hazrat Abolfazl Health and Medical Charity in 2015

Introduction: After transplantation, patients require lifelong follow-up care. The present research was conducted to explore effects of the self-management program on the quality of life among renal-transplant patients in Hazrat Abolfazl Health and Medical Charity in Isfahan in 2015. Materials and Methods: This randomized controlled clinical trial had 2 groups involving 3 stages, namely, before, right after, and 3 months after the intervention. It was conducted on 72 kidney-transplant patients. The participants having inclusion criteria were selected using the simple sampling method and were randomly placed in the experimental and control groups. Each group included 36 participants. The chronic disease self-management program (CDSMP) workshop was presented for the experimental group in a 2½-h session held once a week for 6 months. The control group attended a training session on diet. To collect data, the standard Kidney Transplant Questionnaire was used. Data analysis was performed using SPSS. Results: After the intervention, the means of the quality of life among the patients in the experimental group were 3.82 ± 1.03, 4.49 ± 0.81, and 5.12 ± 0.62 before, right after, and 3 months after the intervention, respectively (P 0.05). Conclusion: Due to the positive effect of the CDSMP on the quality of life among the kidney-transplant patients, it is recommended that authorities accept the self-management program much more.

Assessment of Severity of Malnutrition in Peritoneal Dialysis Patients via Malnutrition: Inflammatory Score.

Background:Regarding to the complications of malnutrition in dialysis patients, using an easy and reliable method for evaluating of malnutrition is important in patients with the end-stage renal disease. Based on the effect of inflammatory factors in malnutrition, A new scale has been designed which is called malnutrition–inflammatory scale (MIS). We designed current study to assess the severity of malnutrition in peritoneal dialysis patients in Isfahan via MIS.Materials and Methods:In this cross-sectional MIS was used for evaluation of malnutrition. MIS includes 10 components: dry weight changes, dietary intake, functional capacity, comorbidity, muscle wasting and loss of subcutaneous fat as well as body mass index (BMI), serum albumin level and total iron binding capacity (TIBC). Each component has four levels of severity from 0 (normal) to 3 (severely abnormal). All analyses were performed using Statistical Package for Social Sciences version 20 (SPSS 20) and P < 0.05 were considered statistically significant.Results:Results showed no significant difference in MIS between male and female participants. Mean of minimum inhibitory concentration in this study was calculated about 4.1 (MIS <9) which means no or mild malnutrition. Significant correlation between MIS and weight (P < 0.001), BMI (P < 0.001), TIBC (P < 0.001), triglyseride (P = 0.04) and arm circumference (P < 0.001) was seen.Conclusion:We suggest That MIS is being used as a valuable tool for prevention of fatal outcomes in chronic dialysis patients.

Neuromyelitis optica spectrum disease characteristics in Isfahan, Iran: A cross-sectional study.

Background:Neuromyelitis optica spectrum disease (NMOSD) is a severe autoimmune demyelinating disorder of the central nervous system that throughout epidemiological data, it has not been completely determined. The aim of this study was to assess characteristics of NMOSD patients in Isfahan as one of the most prevalent cities for multiple sclerosis in Iran.Materials and Methods:Forty-five patients diagnosed as neuromyelitis optica (NMO) disease through 5 years enrolled in this study. Demographics and characteristics of disease such as Expanded Disability Status Scale (EDSS) score, disease duration, clinical symptoms, laboratory data, and magnetic resonance imaging findings (including T1, T2, and flair protocols) were recorded. NMO-immunoglobulin G serology assay was done in all of the patients by ELISA test.Results:Female to male ratio was 5.4:1. The mean age of disease onset was 29.8 ± 11.2 years. NMO antibody was positive in 24.4% of patients. The presenting symptoms were optic neuritis (55.5%), transverse myelitis (40%), and brainstem symptoms (4.5%). The interval between the first and second attack was 19.28 ± 31.27 months (range: 1 month to 17 years). The mean EDSS score of the patients was 2.8 ± 2.25. Frequency of long-extending cervical plaque was higher among men than women (85.7% vs. 57.9%).Conclusion:Based on this study, the mean age of NMOSD onset among Isfahan population was considerably lower than other studies, and there was higher frequency of long-extending cervical lesion among male patients which needs more consideration in further studies.

Comment on "Prevalence of Occult Hepatitis B Virus Infection in Hemodialysis Patients in Isfahan, Iran".

Comment on "Prevalence of Occult Hepatitis B Virus Infection in Hemodialysis Patients in Isfahan, Iran".

The Effect of Contextual Factors on Health Information–Seeking Behavior of Isfahan Diabetic Patients

ABSTRACTThe aim of this study was to examine the effect of contextual factors on the health information–seeking behavior of diabetic patients in Isfahan. This is an applied study using the survey method. Three hundred sixty-two patients were selected using stratified random sampling. Longo’s information-seeking behavior questionnaire was used to collect data and SPSS 20 software was used to analyze data. Pearson correlation index showed that by controlling age, there was a direct relation between longevity of diagnosis and some information-seeking factors (P < .05, r > 0). One-way analysis of variance (ANOVA) test showed that the average score of some factors of diabetic patient’s information-seeking behavior during pregnancy are significantly higher than patients with prediabetes and diabetes (P < .05). Independent t test showed a statistically significant difference between information-seeking behavior of patients with a family history of diabetes and patients without a family history of diabetes (P < .05). Therefore, due to the importance of health care in diabetic pregnant women and prediabetic patients, necessary steps need to be taken to provide these patients with suitable information sources. Also, investigating the factors affecting health information–seeking behavior of patients with chronic disease will help understand and satisfy their information needs, leading to the improvement of the patients’ self-care and prevent complications.

Prevalence of occult hepatitis B virus infection in hemodialysis patients in Isfahan, Iran.

Background:The absence of a detectable hepatitis B surface antigen (HBsAg) with or without hepatitis B core antibody (anti-HBc) or hepatitis B surface antibody (anti-HBs) in the presence of hepatitis B virus-DNA (HBV-DNA) is defined as occult HBV infection. This study was aimed to evaluate the prevalence of occult HBV infection in patients receiving hemodialysis (HD) in Isfahan, Iran.Materials and Methods:This cross sectional study was done on 400 patients without acute or chronic HBV infection with end-stage renal disease undergoing regular HD. Blood samples were collected prior to the HD session, and serological markers of viral hepatitis B included HBsAg, anti-HBs and anti-HBc were measured using standard third generation commercially available enzyme immunoassays kit, then samples of positive anti-HBc and negative anti-HBs were tested for HBV DNA using quantitative real-time polymerase chain reaction techniques. Data were analyzed by SPSS using t-test and Chi-square test.Results:The mean age of patients was 51.6 ± 11.2 years. Anti-HBc positive was observed in 32 (8%) of 400 studied patients with negative HBsAg. Of 32 patients with anti-HBc positive, 15 were males and 17 were females with mean age of 49.7 ± 12.6 years. Among 32 patients with anti-HBc positive, 10 patients were negative for anti-HBs. All of 10 patients were negative for HBV DNA. The prevalence of occult HBV infection was 0%.Conclusions:The prevalence of occult HBV infection in HBsAg negative patients undergoing HD was 0% and look to be among the lowest worldwide. So, occult HBV infection is not a significant health problem in HD patients in this region.

The environmental risk factors in multiple sclerosis susceptibility: A case-control study.

Background:Multiple sclerosis (MS) is a demyelinating and degenerating disease which involves central nervous system. Environmental risk factors have a key role in MS susceptibility. Here we aim to investigate different risk factors effect on MS susceptibility in a large population of MS patients in Isfahan, Iran.Materials and Methods:This study is a cross-sectional hospital-based study, which was conducted on a large group of MS patients registered in Kashani hospital and a control group from normal healthy population. Demographic data, age at onset of the disease, history of viral infections, vaccination, history of trauma to head, recent stressful events, alimentation, familial history, method of delivery (caesarean section, normal vaginal delivery), disability score and history of smoking were gathered using a designed questionnaire.Results:Totally 536 MS patient with the mean age of 34.37 ± 9.22 and 399 individuals from healthy population with the mean age of 32.53 ± 9.91 were recruited. Significant difference in history of measles infection (control = 15.5%, case = 22.4%, P = 0.009), consumption of dairy products (case = 56.6%, control = 67.5%, P = 0.01) and major stressful life events (case = 62.2%, control = 52.7%, P < 0.05) between these two groups were demonstrated.Conclusion:A significant relation between stress, history of infection and milk consumption was reached that highlights the importance of environmental risk factors in MS pathogenesis.

An investigation into the factors effective in the consent of families with brain-dead patients candidates for organ donation in Isfahan, Iran in 2012-13.

Background:Studies have shown that, with regard to social, cultural, and institutional contexts, several factors affect family decision-making on organ donation. This study aimed to investigate the effective factors in organ donation by family members with brain-dead patients.Materials and Methods:This was a descriptive-comparative study in which a researcher-made questionnaire was used to collect data. The reliability of the questionnaire was obtained as 0.81 using Cronbach's alpha. The study sample consisted of 85 members of families with brain-dead patients in Isfahan, Iran in 2012–13. The collected data were analyzed using the Statistical Package for the Social Sciences version 20.0, and the level of significance was considered as <0.05.Results:The obtained results indicated that factors such as age, marital status, level of education, and cause of brain death did not have any effect on their families consent, whereas factors such as gender, duration of hospitalization, having an organ donation card, personal view of the brain-dead patient, and the number of patient's children had a significant relationship with the consent on organ donation. In addition, the care and treatment team were effective in family decisions regarding organ donation.Conclusions:In general, the necessary culture and increasing the population awareness and their knowledge can be a positive step in this regard and may bring about an easy and rapid acceptance of organ donation by the involved families.

Detection of Parvovirus B19 Infection in Thalasemic Patients in Isfahan Province, Iran.

Background:Parvovirus B19, a member of the Erythrovirus genus of Parvoviridae family, causes various clinical illnesses including infectious erythema, arthropathy, hydrops fetalis or congenital anemia, and transient aplastic crises. The B19 virus can be transmitted through respiratory secretions, blood products, and blood transfusion.Objectives:The aim of this study was to detect the B19 virus in thalassemia patients in Isfahan, Iran.Patients and Methods:The prevalence of parvovirus B19 infection was compared between thalassemia major patients and healthy subjects. Plasma samples were collected from 30 thalassemia patients from Isfahan, Iran. Thirty patients without any blood complications were considered as the control group. After DNA extraction from the plasma samples, polymerase chain reaction was performed for parvovirus B19 detection.Results:The parvovirus B19-specific nucleotide sequence was detected in 6 patients (20%). None of the samples obtained from the 30 control subjects tested positive for B19.Conclusions:In this study B19-Parvovirus infection were detected in patients with hematologic disorders in comparison with control subjects. Screening of patients with a high risk of parvovirus B19 infection can considerably reduce the incidence and prevalence of B19 infection.