This paper describes the effect of optical light on the generation and manipulation of microvortex flow named ‘twin opposing microvortex’ (TOMV) flow. This opto-electrohydrodynamic (OEHD) technique combines optical light, i.e. infrared (IR) laser (1064 nm), with non-uniform AC electric fields generated from a pair of indium tin oxide (ITO) electrodes. When the IR laser beam passes through the electric fields, a rapid and three-dimensional (3D) vortex flow is generated in a microchamber. When the laser beam passes through the electric fields, especially the exposed ITO electrode, the direction of the TOMV flow as well as its strength are controlled. With an AC signal of 107 kHz and various voltages below a peak-to-peak voltage of 10 V, laser power is varied up to 1.5 W and the path of a laser beam relative to the electrode (300 μm long and 16 μm wide) is manipulated. The maximum in-plane velocity outside the electrode region was obtained by micron-resolution particle image velocimetry (μPIV). When the laser beam passes through the left or right side of the lower electrode, the TOMV flow field rotates counterclockwise or clockwise, respectively. Applying optical light on an ITO electrode creates in situ and on-demand microvortex flow, which increases the feasibility of OEHD technique in various biological and chemical applications (e.g., mixing and delivering nanofluids in microfluidic devices).