Paternal Influence Research Articles

Does paternal care influence mate preference? Male and female mating behavior in Threespine Stickleback ecotypes that differ markedly in parental care.

Reproductive isolation can occur due to divergence in sexual selection for particular traits. For example, differences in mate preference associated with body size can play an important role in divergence between groups. The importance of mate preference for population divergence may be influenced by other aspects of a mating system, such as the requirement for parental care. In Nova Scotia, Canada, two ecotypes of marine Threespine Stickleback occur sympatrically: a "common" ecotype wherein males provide parental care, and a "white" ecotype that does not exhibit paternal care. The goal of our study was to examine differences in male mate preference between white and common stickleback males to test the prediction that males who invest more in parental care may be more selective about their mates. Because of the link between size and fecundity in this species, we predict that males that invest in parental care should prefer large females, while males that do not provide care will not exhibit preference for larger female size. We found that common male stickleback preferred larger-bodied females of both ecotypes, while white males showed a preference for larger-bodied common females. Secondarily, we assessed whether females differed in their willingness to mate with males of different sizes and ecotypes. Common female stickleback had a higher response rate toward smaller white males, which may be associated with their relatively high courtship rates. Counter to previous studies on these ecotypes that suggest that mating is completely assortative, interecotype matings occurred in half of the observed spawning events. This observation, coupled with the results that males may prefer females based mainly on size and females respond to males who court more rigorously regardless of their ecotype, may lend insight into recent genetic evidence for hybridization in the wild.

Associations of parental attitudes and health behaviors with children’s screen time over four years

BackgroundParents are significantly important in shaping the screen use of children within a family system. This study aimed to examine the associations of Chinese children’s screen time (ST) over four years with parents’ attitudes toward their own screen use and physical activities (PA) and health behaviors including their ST, PA, cigarette smoking, and alcohol drinking.MethodsThe current study utilized data from two waves (2011 and 2015) of the China Health and Nutrition Survey (CHNS), including 1,941 mother-father-child triads in 2011 and 2,707 mother-father-child triads in 2015 (with children aged 0-17-years-old). The ST of children and the parental attitudes and health behaviors were measured via self-report or proxy-report (for children under 6 years old) questionnaires. Pool-OLS regression models were used to assess the associations of parental attitudes and health behaviors with the ST of children. Moderation models were built to assess whether these associations depended on the gender, age, and family income of children, as well as whether paternal and maternal influences were moderated by the other parent. A multilevel cross-lagged panel model (CLPM) was used to assess parental influences on children’s ST over four years.ResultsPaternal ST (β = 0.09, p < 0.001), maternal ST (β = 0.10, p < 0.001), and paternal alcohol drinking (β = 0.30, p < 0.05) were positively associated with children’s ST. In addition, maternal smoking had a positive association with girls’ ST (β = 0.53, p < 0.05). Moreover, the association between maternal ST and children’s ST was observed to decline as family income increased (β = -0.03, p < 0.001). Paternal ST had a larger positive association with children’s ST when the ST of mothers exceeded 14 h/week (β = 0.06, p < 0.05). Furthermore, lagged associations were found between paternal attitudes toward PA (β = -1.63, p < 0.05) or maternal cigarette smoking (β = 1.46, p < 0.05) and children’s ST measured four years later.ConclusionChildren establish a healthy lifestyle within the family system. From the perspective of the healthy family climate, the current study suggests that future programs for reducing children’s ST should be built through an integrative approach with special attention to parental attitudes and health behaviors.

The Unique Role of Father Involvement in Child Socioemotional Development

The role of fathers in the modern households has significantly changed, prompting scholarly interest in the uniqueness of paternal influences. The early childhood development literature is thereby no longer limited to focus solely on the effects of maternal behaviors. In particular, father involvement is regarded to have distinct mechanisms of influence on children's early social-emotional development that relatively independent of motherhood. However, there is not even a consistent definition of father involvement yet, and the current literature has received little attention within the pathways of how its influence on early childhood development. To bridge this gap, this paper aims to explore aspects of father involvement and the mechanisms of its impact on children's social-emotional behavior. Grounded on attachment theory and activation relationship theory, this paper presents that father involvement can build high-quality father-child attachments both through sensitive affection engagement and the challenges and activations that common in physical play, thus having a unique impact on children's social-emotional behavior that is different from that of their mothers. This paper can serve as an inspiration for future directions in father involvement research and has potential practical implications for policymakers and families with newborns. There remains ambiguities and gaps in the research on the parenting literature, such as there are very few studies have yet considered the dynamic and reciprocal nature of the father-child relationship, which need to be addressed by future research.

Characterisation of the Paternal Influence on Intergenerational Offspring Cardiac and Brain Lipid Homeostasis in Mice.

There is growing evidence that poor paternal diet at the time of conception increase the risk of offspring developing a range of non-communicable metabolic diseases, such as obesity, diabetes and cardiovascular disease, in adulthood. We hypothesise that a paternal low protein-high carbohydrate diet perturbs offspring tissue lipid abundance through both sperm and seminal plasma-mediated mechanisms. To test our hypothesis, we fed male C57BL/6 mice either a control normal protein diet (NPD; 18% protein) or an isocaloric low protein diet (LPD; 9% protein) for a minimum of 8 weeks. We generated offspring through artificial insemination, in combination with vasectomised male mating. Using this approach, we derived offspring from either NPD or LPD sperm but in the presence of NPD or LPD seminal plasma. Using high resolution mass-spectrometry, we found that offspring derived from either LPD sperm or seminal fluid displayed perturbed cardiac and brain lipid abundance from just three weeks of age, typically associated with the altered abundance of tissue triglycerides. We also observed the differential sex-specific patterns of lipids between the control and experimental offspring's hearts and brains. These observations indicate that poor paternal diet at the time of conception affects offspring cardiac and brain lipid profiles in an age-, sex- and generation-specific manner.

Paternal determinants of early embryo development

Existing research has primarily focused on investigating the impacts of the maternal environment, female fertility phenotype, and genetics on pregnancy loss in dairy cattle. Recently, attention has been directed toward understanding the role the sire has on embryo quality and viability. Studies have shown there is a paternal influence on early pregnancy loss, but the specific mechanisms impacting pregnancy establishment and maintenance remain unclear. Despite clear differences that sires have on pregnancy outcomes, there is a lack of evidence regarding specifically how sires influence pregnancy. Sperm characteristics, such as motility, concentration, and morphology, have been extensively studied, but further research is needed to understand what makes one sire more or less fertile than another sire and how this affects pregnancy. To effectively address pregnancy loss, a deeper understanding of the processes involved from fertilisation to blastocyst formation is essential, particularly for understanding early pregnancy loss.

Paternal epigenetic influences on placental health and their impacts on offspring development and disease.

Our efforts to understand the developmental origins of birth defects and disease have primarily focused on maternal exposures and intrauterine stressors. Recently, research into non-genomic mechanisms of inheritance has led to the recognition that epigenetic factors carried in sperm also significantly impact the health of future generations. However, although researchers have described a range of potential epigenetic signals transmitted through sperm, we have yet to obtain a mechanistic understanding of how these paternally-inherited factors influence offspring development and modify life-long health. In this endeavor, the emerging influence of the paternal epigenetic program on placental development, patterning, and function may help explain how a diverse range of male exposures induce comparable intergenerational effects on offspring health. During pregnancy, the placenta serves as the dynamic interface between mother and fetus, regulating nutrient, oxygen, and waste exchange and coordinating fetal growth and maturation. Studies examining intrauterine maternal stressors routinely describe alterations in placental growth, histological organization, and glycogen content, which correlate with well-described influences on infant health and adult onset of disease. Significantly, the emergence of similar phenotypes in models examining preconception male exposures indicates that paternal stressors transmit an epigenetic memory to their offspring that also negatively impacts placental function. Like maternal models, paternally programmed placental dysfunction exerts life-long consequences on offspring health, particularly metabolic function. Here, focusing primarily on rodent models, we review the literature and discuss the influences of preconception male health and exposure history on placental growth and patterning. We emphasize the emergence of common placental phenotypes shared between models examining preconception male and intrauterine stressors but note that the direction of change frequently differs between maternal and paternal exposures. We posit that alterations in placental growth, histological organization, and glycogen content broadly serve as reliable markers of altered paternal developmental programming, predicting the emergence of structural and metabolic defects in the offspring. Finally, we suggest the existence of an unrecognized developmental axis between the male germline and the extraembryonic lineages that may have evolved to enhance fetal adaptation.

Moving from the Periphery: Latinx Fathers’ Influence on Their Daughters’ Persistence in Engineering

ABSTRACT The influential role of familía remains a crucial aspect to understand Latinxs’ success in postsecondary schooling. Rather than relying on a deficit lens, this study employs an asset-based lens to gain insight into the paternal influence that assists Latinas’ persistence to degree attainment in engineering. More specifically, this study examines the crucial role that fathers play in the familía as it pertains to Latina persistence in engineering programs. A conceptual framework of Community Cultural Wealth and Latinx Cultural Pedagogics undergird this case study. The themes that emerged from data analysis include (a) Fathers Living Vicariously Through Their Daughters, (b) Fathers Serving as Career Role Models and (c) Fathers Providing Unconditional Support. The authors also offer recommendations for future research.

Kangaroo Fathers Modulate Maternal Control of Offspring Sex but Not Postpartum Maternal Expenditure.

AbstractWhen sons and daughters have different fitness costs and benefits, selection may favor deviations from an even offspring sex ratio. Most theories on sex ratio manipulation focus on maternal strategies and sex-biased maternal expenditure. Recent studies report paternal influences on both offspring sex ratio and postpartum sex-biased maternal expenditure. We used long-term data on marked kangaroos to investigate whether and how paternal mass and skeletal size, both determinants of male reproductive success, influenced (a) offspring sex in interaction with maternal mass and (b) postpartum sex-biased maternal expenditure. When mothers were light, the probability of having a son increased with paternal mass. Heavy mothers showed the opposite trend. A similar result emerged when considering paternal size instead of mass. Postpartum maternal sex-specific expenditure was independent of paternal mass or size. Studies of offspring sex manipulation or maternal expenditure would benefit from an explicit consideration of paternal traits, as paternal and maternal effects can modulate each other.

Intention to Breastfeed and Paternal Influence on Pregnant Mothers Exclusively Using Marijuana Compared with Other Substances.

Objective: To determine intention to breastfeed (ITBF) rates among mothers exclusively using marijuana (eMJ) compared with electronic cigarettes (eEcig), tobacco products (eTob), or multisubstances (MS), nonusers (NU), and the influence of paternal presence and paternal substance use. Study Design: Cross-sectional study of parental survey responses merged with electronic birth certificates. Accounting for clinical and social determinants of health, analyses of ITBF included (1) all mothers, (2) single mothers, and (3) mothers with fathers. Results: Among all mothers (n = 1,073), eMJ, eTob, and MS users had lower odds of ITBF compared with NU. Only eMJ users had lower odds of ITBF for those without paternal presence. However, in those mothers with a paternal presence, odds of ITBF were similar to NU for eMJ, eTob, and MS users when accounting for paternal factors, including paternal substance use. Conclusion: Women exclusively using MJ have lower ITBF compared with NU. However, paternal presence mitigated this effect, independent of parental MJ use. The presence of fathers may represent a unique predictor for increased ITBF in MJ using mothers.

The Legacy of Parental Obesity: Mechanisms of Non-Genetic Transmission and Reversibility.

While a dramatic increase in obesity and related comorbidities is being witnessed, the underlying mechanisms of their spread remain unresolved. Epigenetic and other non-genetic mechanisms tend to be prominent candidates involved in the establishment and transmission of obesity and associated metabolic disorders to offspring. Here, we review recent findings addressing those candidates, in the context of maternal and paternal influences, and discuss the effectiveness of preventive measures.

SIGNIFICANT EFFECT OF PATERNAL AGE ON CUMULATIVE LIVE BIRTH RATES (CLBR) PER INSEMINATED OOCYTE, EMBRYO TRANSFER (ET) AND EMBRYO TRANSFERRED IN IVF-ICSI WITH AUTOLOGOUS OOCYTES

In recent years, there has been an increase of the average paternal age at which the first child is conceived. Studies addressing paternal age and reproductive outcomes frequently show controversial results, probably because of the wrong statistical approach. We aimed to assess paternal age influence on reproductive outcomes in terms of CLBR per inseminated oocyte, ET and embryo transferred until the first live birth (LB) is achieved in autologous IVF-ICSI to provide with a more precise estimation after considering the overall contribution of the embryo cohort.

Situating the Father: Strengthening Interdisciplinary Collaborations between Sociology, History and the Emerging POHaD Paradigm.

(1) Background: Albeit the main focus remains largely on mothers, in recent years Developmental Origins of Health and Disease (DOHaD) scientists, including epigeneticists, have started to examine how a father’s environment affects disease risk in children and argued that more attention needs to be given to father’s health-related behaviors for their influence on offspring at preconception (i.e., sperm health) as well as paternal lifestyle influences over the first 1000 days. This research ushers in a new paternal origins of health and disease (POHaD) paradigm and is considered a welcome equalization to the overemphasis on maternal influences. Epigeneticists are excited by the possibilities of the POHaD paradigm but are also cautious about how to interpret data and avoid biased impression of socio-biological reality. (2) Methods: We review sociological and historical literatures on the intersection of gender, food and diet across different social and historical contexts to enrich our understanding of the father; (3) Results: Sociological and historical research on family food practices and diet show that there are no “fathers” in the abstract or vacuum, but they are differently classed, racialized and exist in socially stratified situations where choices may be constrained or unavailable. This confirms that epigeneticists researching POHaD need to be cautious in interpreting paternal and maternal dietary influences on offspring health; (4) Conclusions: We suggest that interdisciplinary approach to this new paradigm, which draws on sociology, history and public health, can help provide the social and historical context for interpreting and critically understanding paternal lifestyles and influences on offspring health.

Black Fathers Rising: A QuantCrit Analysis of Black Fathers’ Paternal Influence on Sons’ Engagement and Sense of School Belonging in High School

ABSTRAC This QuantCrit analysis considers Black fathers’ influence on their sons’ engagement and sense of belonging in urban intensive and emergent high schools. Canonical correlations show a significant relationship between Black fathers’ paternal influence and their sons’ engagement and sense of belonging in school. We discuss two QuantCrit interpretation strategies to contextualize and recenter race via Black fathers’ experiential knowledge. Within the proper social-cultural context, these results illuminate a unique paternal educational habitus among Black fathers, which focuses on high levels of academic engagement with a family-centric locus of influence on their sons’ sense of belonging.

O-297 Does advanced paternal age influence live birth rate independent of woman’s age: analysis of 18, 825 fresh IVF/ICSI cycles from a national (HFEA) database

Abstract Study question Does advanced paternal age (APA) impact live birth rates (LBRs) in IVF/ICSI treatment independent of female partner’s age? Summary answer LBRs decline with paternal age ≥40 years, but not when female partner is &amp;lt; 35 or ≥ 40 years. What is known already APA, particularly over 40 years, has been shown to be associated with declining sperm parameters, impaired embryo development, aneuploidy, compromised pregnancy outcomes and mental health abnormalities in the offspring in IVF/ICSI cycles. However, no significant effect of APA on pregnancy outcomes has been noted in donor oocyte cycles, indicating possible positive effect of female age counteracting negative male age-related IVF/ICSI outcomes. Data on whether female and male age influence each other, or are independently related to LBR, are limited and inconclusive. Study design, size, duration Retrospective analysis of 18, 825 autologous IVF or ICSI with fresh single embryo transfer (SET) cycles from the UK’s national anonymised registry, published online by the Human Fertilisation and Embryology Authority (HFEA). Study period: 2 years starting from 1st January 2017 to 31st December 2018. Participants/materials, setting, methods Couples underwent IVF/ ICSI with fresh SET due to unexplained or tubal infertility. Cycles with male factor, donor sperm and preimplantation genetic testing were excluded. Female age was grouped to &amp;lt; 35 years, 35-39 years and 40-44 years; male age to &amp;lt; 35 years (control group), 35-39 years, 40-44 years, 45-50 years, 51-55 years and &amp;gt;55 years. Chi-square to compare binominal data; multiple logistic regression to find correlation of male and female age on LBR adjusting confounders. Main results and the role of chance Overall, LBR per SET declined from 39.1% in men &amp;lt;35 years (control group) to 25.9% (p &amp;lt; 0.0001), 23.0% (p &amp;lt; 0.0001), 22.9% (p &amp;lt; 0.0001) and 25.4% (p = 0.002) in 40-44, 45-50, 51-55 and &amp;gt;55 years, respectively. However, in the subgroups of women &amp;lt;35 years, the LBRs were no different whether men were of &amp;lt; 35 (41.3%), 40-45 (40.8%) or &amp;gt; 55 (41.7%) years. Similar trend was observed in women aged 40-44 years: LBRs were 12.3%, 11.9%, and 17.4% in men aged &amp;lt;35, 40-44, and &amp;gt;55 year, respectively. Only in women aged 35-40 years, LBRs dropped from 32.8% in men aged &amp;lt;35 years down to 27.9% (p = 0.004), 28.1% (p = 0.04), 28.7% (p = 0.33) and 25% (p = 0.22) in 40-44, 45-50, 51-55 and &amp;gt;55 years, respectively. The findings remained the same with ICSI only cycles. Individually, male age, female age, treatment-type (IVF or ICSI) and day of transfer were significantly correlated with LBR. Overall, after adjusting for confounders, both male (p = 0.01) and female’s age (p &amp;lt; 0.0001) remained independently related to LBRs.; male age did not correlate with LBRs in women aged &amp;lt;35 years or 40-44 years. Limitations, reasons for caution Information on ovarian reserve and treatment protocols were not available. However, this may have little impact, given large population studied. Exclusion of male infertility might have reduced the ‘effect-size’ but was important to eliminate non-age-related male factors, in order to assess only men’s age on the pregnancy outcome. Wider implications of the findings Oocytes in women &amp;lt;35 appear to reduce the negative impact of older sperm on LBR, but not in women 35-39 years of age. This is useful information when counselling couples and in fertility education setting. Further research is needed to clarity the mechanism underlying our findings. Trial registration number Does advanced paternal age influence live birth rate independent of woman’s age: analysis of 18, 825 fresh IVF/ICSI cycles from a national (HFEA) database

The intergenerational transmission of gender: Paternal influences on children's gender attitudes

AbstractObjectiveThis study provides the first systematic longitudinal analysis of the influence of paternal involvement in family life—across childhood and adolescence—on the gender‐role attitudes of children by the age of 14 or 15.BackgroundRecent research suggests that, in post‐industrial societies, paternal involvement in family life is increasing. Although previous studies of paternal involvement have considered paternal influences on children's cognitive or socio‐emotional development, such studies have not yet addressed paternal influences on children's attitudes toward gender. Relatedly, previous studies on the intergenerational transmission of gender attitudes have analyzed maternal influences, but have neglected the significance of paternal influences. This study engages both strands of the research by analyzing the effects of paternal behaviors on children's attitudes toward gender roles.MethodMultivariate linear regressions models were estimated on data from the Longitudinal Study of Australian Children (LSAC); a survey with biannual observations over 10 years for 2796 children born between 1999 and 2000.ResultsFathers' time spent on childcare during childhood was associated with gender‐egalitarian attitudes in children by the age of 14 or 15. The most powerful predictor of children's gender‐role attitudes, however, was the amount of time fathers spent on housework during children's adolescence, both absolute and relative to the amount of time mothers spent on housework. Fathers' unpaid labor at home was as relevant for children's gender‐role attitudes as mothers' paid labor in the workforce. These results held after controlling for maternal domestic behaviors and for the gender‐role attitudes of both parents.ConclusionFather involvement in childcare and housework during childhood and adolescence play an important role in shaping children's gender‐egalitarian attitudes.

In the name of the Gothic father: François Truffaut’s L’Histoire d’Adèle H. (1975)

ABSTRACT The reception of François Truffaut’s 1975 film L’Histoire d’Adèle H., dealing with the obsession and descent into madness of the daughter of Victor Hugo, has recurrently focused on its critical engagement with Romantic melodrama, as well as its literary and ‘romanesque’ dimensions. This article argues that the term ‘romanesque’ has camouflaged the film’s active engagement with both cinematic and literary Gothic heritage. This engagement is shown to be inextricably linked to dynamic tensions of paternal influence. Both Truffaut as director and Adèle Hugo as film character actively renegotiate the Father’s artistic heritage of violent passions and dark metaphysical forces, with differing results. The article proposes a double analysis: (a) a close reading of the film’s Gothic tropes, shedding light on Truffaut’s intertextual dialogue with Hitchcock, his favourite ‘Gothic’ father and (b) an examination of the character Adèle H.’s intertextual performance, in which she contends with the powerful Gothico-Romantic myth embodied by the literary patriarch Victor Hugo. This will lead to extended insight into Truffaut’s brand of filmic Gothic as a vehicle of haunted intensity and on his distinctive exploration of the torments of female subjectivity, haunting and neurosis.

Nutrient content of sorghum hybrid lines between Gadam and hard coat tannin sorghum cultivars.

Sorghum is an important food crop in the world that exhibits a predominant role in fulfilling the nutritional requirements, particularly in low‐income group populations of marginal areas in Kenya. It is a principal source of proteins, carbohydrates, fats, and crude fibers (CFs), which are important nutrients necessary for human development and health. Reduced tannin in sorghum grains is desirable since it affects the availability of nutrients. This study aimed at assessing the nutrient content in filial generation one (F1) developed between Gadam (sorghum), which is low in tannin and hard coat tannin (sorghum) cultivars. The nutrient content analyses were carried out from samples collected in a completely randomized design experiment. Crude protein (CP) and tannin content were analyzed using the modified Kjeldahl method and vanillin‐HCl methanol method, respectively, whereas moisture, fat, CF, ash, and carbohydrate contents were determined using Association of Official Analytical Chemists methods. Data collected were subjected to analysis of variance using R statistical software. Among the F1s, Kari/Mtama‐1 x Gadam recorded the highest CP value of 10.390%. This differed significantly from Gadam x Kari/Mtama‐1 which recorded CP content of 9.770%. Kari/Mtama‐1 x Gadam recorded the highest fat and moisture contents of 2.299% and 8.600%, respectively. The highest CF content of 3.433% was recorded in Gadam x Serena. Gadam x Kari/Mtama‐1 recorded the highest ash content of 1.619%, whereas the highest carbohydrate (84.503%) and tannin content (0.771 mg/g) means were recorded in Seredo x Gadam. Results demonstrated that the choice of maternal and paternal parent influence CP, CF, and carbohydrate contents. Among the F1s, tannin content ranged from 0.106 to 0.771 mg/g compared to 0.953 to 1.763 mg/g recorded in Serena and Seredo (hard coat seeded cultivars). This is an indication that tannin can be downregulated through hybridization.

Familial transmission of attention allocation towards one's own and a peer's body: An eye-tracking study with male adolescents and their fathers.

Previous research provides evidence of maternally transmitted body-related attentional biases in female adolescents. In contrast, it remains unclear whether a familial transmission of body-related attentional biases also exists within father-son dyads. Therefore, the current study examined n = 42 male adolescents and their fathers with respect to direct and indirect paternal influences on body-related attention patterns and specific body-related concerns in sons. Besides completing specific body image questionnaires, participants were shown pictures of their own and a respective peer’s body, while their eye movements were tracked. The fathers additionally viewed the body pictures of their own son and an adolescent peer. Contrary to the assumed direct and indirect paternal transmission processes, the sons’ body-related attention patterns were not significantly associated with the perceived amount of paternal body-related feedback, with the fathers’ attention patterns towards their own son’s and the adolescent peer’s body, or with the fathers’ attention patterns towards their own and the adult peer’s body. Similarly, no significant associations were found between direct or indirect paternal influences and the sons’ drives for muscularity and thinness, body dissatisfaction, and muscularity-related body-checking behavior. Comparing the present findings with previous research indicating a maternal transmission of body-related attentional biases and body-related concerns in female adolescents, alternative (not gender-linked) familial transmission processes, e.g., via one’s own mother, or a comparatively higher relevance of other sociocultural influences, e.g., via peers or the media, might be assumed for male adolescents.

Gene Expression in Embryos From Norwegian Red Bulls With High or Low Non Return Rate: An RNA-Seq Study of in vivo-Produced Single Embryos.

During the last decade, paternal effects on embryo development have been found to have greater importance than previously believed. In domestic cattle, embryo mortality is an issue of concern, causing huge economical losses for the dairy cattle industry. In attempts to reveal the paternal influence on embryo death, recent approaches have used transcriptome profiling of the embryo to find genes and pathways affected by different phenotypes in the bull. For practical and economic reasons, most such studies have used in vitro produced embryos. The aim of the present study was to investigate the differences in the global transcriptome of in vivo produced embryos, derived from sires with either high or low field fertility measured as the non-return rate (NRR) on day 56 after first AI of the inseminated cows. Superovulated heifers (n = 14) in the age span of 12–15 months were artificially inseminated with semen from either high fertility (n = 6) or low fertility (n = 6) bulls. On day seven after insemination, embryos were retrieved through uterine flushing. Embryos with first grade quality and IETS stage 5 (early blastocyst), 6 (blastocyst) or 7 (expanded blastocyst) were selected for further processing. In total, RNA extracted from 24 embryos was sequenced using Illumina sequencing, followed by differential expression analysis and gene set enrichment analysis. We found 62 genes differentially expressed between the two groups (adj.p-value<0.05), of which several genes and their linked pathways could explain the different developmental capacity. Transcripts highly expressed in the embryos from low fertility bulls were related to sterol metabolism and terpenoid backbone synthesis, while transcripts highly expressed in the high fertility embryos were linked to anti-apoptosis and the regulation of cytokine signaling. The leukocyte transendothelial migration and insulin signaling pathways were associated with enrichments in both groups. We also found some highly expressed transcripts in both groups which can be considered as new candidates in the regulation of embryo development. The present study is an important step in defining the paternal influence in embryonic development. Our results suggest that the sire’s genetic contribution affects several important processes linked to pre-and peri implantation regulation in the developing embryo.

Recurrence of postpartum hemorrhage, maternal and paternal contribution, and the effect of offspring birthweight and sex: a population-based cohort study

PurposeThis study examines individual aggregation of postpartum hemorrhage (PPH), paternal contribution and how offspring birthweight and sex influence recurrence of PPH. Further, we wanted to estimate the proportion of PPH cases attributable to a history of PPH or current birthweight.MethodsWe studied all singleton births in Norway from 1967 to 2017 using data from Norwegian medical and administrational registries. Subsequent births in the parents were linked. Multilevel logistic regression was used to calculate odds ratios (ORs) with 95% confidence intervals (CI) for PPH defined as blood loss > 500 ml, blood loss > 1500 ml, or the need for blood transfusion in parous women. Main exposures were previous PPH, high birthweight, and fetal sex. We calculated adjusted population attributable fractions for previous PPH and current high birthweight.ResultsMothers with a history of PPH had three- and sixfold higher risks of PPH in their second and third deliveries, respectively (adjusted OR 2.9; 95% CI 2.9–3.0 and 6.0; 5.5–6.6). Severe PPH (> 1500 ml) had the highest risk of recurrence. The paternal contribution to recurrence of PPH in deliveries with two different mothers was weak, but significant. If the neonate was male, the risk of PPH was reduced. A history of PPH or birthweight ≥ 4000 g each accounted for 15% of the total number of PPH cases.ConclusionA history of PPH and current birthweight exerted strong effects at both the individual and population levels. Recurrence risk was highest for severe PPH. Occurrence and recurrence were lower in male fetuses, and the paternal influence was weak.