In vivo measurements of patellofemoral joint contact area are scarce. Patellofemoral contact area has been measured in living people under static conditions with the knee held at fixed angles between 0 and 60° of flexion. No previous study to our knowledge has measured patellofemoral contact area in vivo during dynamic activity. The aim of this study was to measure and compare patellofemoral joint contact area in healthy people across a range of daily activities. Mobile biplane X-ray imaging was used to measure 3D tibiofemoral and patellofemoral kinematics in level walking, downhill walking, stair ascent, stair descent, and open-chain (non-weightbearing) knee flexion and knee extension. The kinematic data were combined with magnetic resonance imaging to determine patellofemoral joint contact area at each time point during each activity. The knee flexion angle explained, respectively, 83%, 80%, and 72% of the variability in the total, lateral, and medial patellofemoral contact areas measured across all participants and all activities. Total, lateral, and medial patellofemoral contact areas increased from 0 to 60° of knee flexion and then decreased as the flexion angle increased further, up to ~ 120°. Patellofemoral contact area was less sensitive to the type of activity and hence joint load. The lateral patellofemoral contact area was larger than the medial patellofemoral contact area throughout the range of knee flexion in all activities (p < 0.001). Knowledge of patellofemoral contact area during daily activities like walking improves our understanding of patellofemoral joint biomechanics and will assist in validating computational models of the patellofemoral joint.
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