Pasteurized Apple Juice Research Articles

Alicyclobacillus spp. in the Fruit Juice Industry: History, Characteristics, and Current Isolation/Detection Procedures

The first Alicyclobacillus spp. was isolated in 1982, and was originally thought to be strictly limited to thermophilic and acidic environments. Two years later, another Alicyclobacillus sp., A. acidoterrestris, was identified as the causative agent in spoilage of commercially pasteurized apple juice. Subsequent studies soon found that Alicyclobacillus spp. are soilborne bacteria, and do not strictly require thermophilic and acidic environments. Alicyclobacillus spp. posess several distinct characteristics; the major one is their ability to survive commercial pasteurization processes and produce off-flavors in fruit juices. The fruit juice industry has acknowledged Alicyclobacillus spp. as a major quality control target microorganism.Guaiacol and halophenols were identified as the offensive smelling agent in many Alicyclobacillus spp. related spoilage. Though the exact formation pathway of these off-flavors by Alicyclobacillus spp. are not yet identified, studies report that the presence of Alicyclobacillus spp. in the medium may be a major contributor to the formation of these off-flavors.Many identification methods and isolation media were developed in the last two decades. However, most of these methods were developed specifically for A. acidoterrestris, which was the first identified off-flavor producing Alicyclobacillus. However, recent studies indicate that other species of Alicyclobacillus may also produce guaiacol or the halophenols. In this respect, all Alicyclobacillus spp. should be monitored as potential spoilage bacteria in fruit juices.This article includes an overall review of the history of Alicyclobacillus spp., characteristics, suggested off-flavor production pathways, and commonly used identification methods for the currently identified Alicyclobacillus spp.

Evaluation of Salmonella typhimurium, Yersinia enterocolitica and Staphylococcus aureus counts in apple juice with cinnamon, by conventional media and thin agar layer method

Pasteurized apple juice (pH 3.64) with cinnamon (0% and 0.3%) was inoculated with Salmonella typhimurium, Yersinia enterocolitica or enterotoxigenic Staphylococcus aureus at 10 4 cfu ml −1, and stored at 5°C and 20°C. Counts on tryptic soy agar (TSA), selective medium and thin agar layer (TAL) were determined at 1 h and 1, 3, 7 and 14 days. The TAL method (selective medium overlaid with TSA) was for recovery of injured cells. In general, the sensitivity of pathogens to cinnamon in descending order was Staph. aureus, Y. enterocolitica and S. typhimurium. Staph. aureus (1.2 log cfu ml −1 at 3 days) and Y. enterocolitica (0.3 log cfu ml −1 at 1 day) were the most acid-sensitive at 5°C and 20°C, respectively. At some points of storage, the killing effect was significantly ( P<0.05) more pronounced at 20°C for the three pathogens, with c. 4 log cfu ml −1 reductions in 1 day in Y. enterocolitica-inoculated samples with and without cinnamon, and in Staph. aureus-inoculated samples with cinnamon. Counts on TSA and TAL did not differ significantly ( P>0.05) in any case. So, both media showed similar effectiveness in recovering damaged cells of the three pathogens. Cinnamon inactivates important foodborne pathogens in apple juice and thus enhances the safety of this product.

Microbiological quality of walnut kernels and apple juice concentrate

In the present study, we have evaluated the microbiological quality of walnut kernels and pasteurized apple juice concentrate and the application of PCR for quality control of these important horticultural products. PCR assays for the detection of Bacillus cereus, Salmonella, Escherichia coli and E. coli O157:H7 were standardized using minimum time for each step of the reaction. The protocols were effective for their detection in these products after pre-enrichment for 6–12 h. 2, 68 and 30% of the samples of walnut kernels were respectively found satisfactory, acceptable and unsatisfactory on the basis of their viable count. Only 15% of the samples of pasteurized apple juice concentrate were found to possess the desired viable count of less than 100 c.f.u./ml. The predominant contaminants of walnut kernels were found to be the species of Bacillus, Klebsiella, Enterobacter and Staphylococcus. Samples of apple juice concentrate were predominantly found contaminated with species of Bacillus, Staphylococcus and Micrococcus. However, B. cereus, Salmonella and E. coli were also isolated from some of the samples of walnut kernels. Bacillus cereus was also obtained from some of the samples of pasteurized apple juice concentrate in high numbers. Among the moulds Penicillium, Aspergillus, Cladosporium, Rhizopus and Mucor were isolated from these products.

Inactivation of Listeria monocytogenes Scott A 49594 in Apple Juice Supplemented with Cinnamon

Normal (pH 3.7) and adjusted (pH 5.0) pasteurized apple juice containing cinnamon (0, 0.1, 0.2, and 0.3%) was inoculated with Listeria monocytogenes Scott A 49594 at 104 CFU/ml and stored at 5 and 20°C for 7 days. Counts on tryptic soy agar (TSA), modified Oxford (MOX) medium, and thin agar layer (TAL) were determined at 1 h and 1, 3, and 7 days. The TAL method (MOX medium overlaid with TSA) was used for the recovery of injured cells. In apple juice, both at normal and adjusted pH, with any doses of cinnamon, no L. monocytogenes (a 4.6-log CFU/ml reduction) was detected after 1 h of storage at both temperatures, and no growth occurred at any points of storage. Therefore, cinnamon by itself (regardless of pH) had a pronounced killing effect. A further enrichment step with brain heart infusion agar showed that L. monocytogenes was completely inactivated in apple juice stored at 20°C, except in pH 5.0 samples with 0.1% of cinnamon. The TAL method was as effective as TSA in recovering injured cells of L. monocytogenes. Cinnamon considerably inactivates L. monocytogenes in apple juice and thus enhances the safety of this product.

Combination of Carbon Dioxide and Cinnamon to Inactivate Escherichia coli O157:H7 in Apple Juice

ABSTRACT: Pasteurized apple juice with CO2 (0, 1, and 4%) and cinnamon (0 and 0.3%) was inoculated with Escherichia coli O157:H7 at 104 CFU/mL, and stored at 5 and 20 °C. Counts on nonselective and selective media, and thin agar layer (TAL; selective medium overlaid with nonselective medium) were determined at 1 h and 1, 3, 7, and 14 d. Inactivation was greater at 20 °C. Samples with 1 and 4% CO2, alone and combined with cinnamon, presented &lt; 0.7 log CFU/mL in 3 d. Counts in apple juice inoculated at 102 CFU/mL, a low‐level E. coli O157:H7 contamination, were nondetectable at 3 d. The TAL method was as effective as nonselective medium to recover injured cells.

Enhanced Recovery of Salmonella from Apple Cider and Apple Juice with Universal Preenrichment Broth

A comparison was made of the relative efficiencies of Universal Preenrichment (UP) broth and lactose broth for the recovery of a variety of Salmonella serovars from pasteurized and unpasteurized apple cider and pasteurized apple juice. Bulk portions of juice were contaminated with single Salmonella serovars at high and low levels of 0.4 and 0.04 CFU/mL, respectively. The juice was aged for a minimum of 5 days at 2-5 degrees C. On the day analysis was initiated, each of 20 test portions (25 mL) of the contaminated juice was preenriched in UP broth and in lactose broth. The Bacteriological Analytical Manual Salmonella culture method was followed thereafter. For pasteurized apple cider, UP broth recovered significantly (p < 0.05) more Salmonella-positive test portions than did lactose broth (112 and 75, respectively). For unpasteurized apple cider, UP broth recovered significantly more Salmonella-positive test portions than did lactose broth (326 and 221, respectively). For pasteurized apple juice, UP broth recovered more Salmonella-positive test portions than did lactose broth (93 and 81, respectively). However, this difference was not statistically significant. These results indicate that UP broth should replace lactose broth for the analysis of pasteurized and unpasteurized apple cider and pasteurized apple juice.

SENSORY AND PHYSIO-CHEMICAL PROPERTIES OF MEMBRANE FILTERED APPLE JUICES

Shelf-stable apple juices were prepared using two ceramic and four polymeric tubular membranes of varying pore sizes and evaluated using color matching and triangle tests. Juices from 9 kDa and 20 kDa membranes were different in color and aroma/flavor from the other membranes, the characteristics of which did not differ from each other. A full sensory profile was obtained for experimental juices produced using a 0.02 μm ceramic membrane and from commercial pasteurized apple juice. Experimental juices were prepared from fresh and stored apples with and without ascorbic acid. Twelve judges evaluated color; cooked/caramelized, appley, fruity and green aromas and seven flavor attributes (cooked/caramelized, appley, fruity, green, sweet, sour, astringence). Analysis of variance and principal component analysis revealed that membrane filtered juices lacked the cooked/caramelized aroma and had a green flavor compared with the commercial apple juice. Membrane filtered juices prepared from freshly harvested apples had less appley and fruity aroma and flavor, but were more sour and astringent than juices prepared from stored apples. Ascorbic acid treatment significantly reduced the yellow color and increased the astringence of juices.

Determination of D-Malic Acid in Apple Juice by Liquid Chromatography: Collaborative Study

Eleven laboratories collaboratively studied a liquid chromatographic (LC) method for determination of D-malic acid in apple juice. The mobile phase consisted of mM L-valine and 8 mM copper acetate adjusted to pH 5.5 with NaOH. The UV detector was set at 330 nm, and a single reversed-phase LC column was used. Seven paired samples containing various amounts of D-malic acid ranging from 0 to 188 mg/100 mL of 12 Brix pasteurized apple juice were tested by each collaborator. Repeatability and reproducibility coefficients of variation ranged from 1.0 to 3.5% and 7.7 to 11.7%, respectively, within the range of 26 to 188 mg D-malic acid/100 mL of 12 Brix apple juice. The collaborative study results demonstrated that the method could quantitate the economic adulteration of apple juice with DL-malic acid at lower levels than those reported with previous methods. The LC method for determination of D-malic acid in apple juice has been adopted first action by AOAC INTERNATIONAL.

Bicarbonate inhibition of Saccharomyces cerevisiae and Hansenula wingei growth in apple juice

The ability of sodium bicarbonate to inhibit growth of Saccharomyces cerevisiae and Hansenula in apple juice was investigated. Sodium bicarbonate at concentrations of 0.06, 0.12, and 0.24 M was added to pasteurized apple juice that was then inoculated with 10 3 cfu/ml of either yeast. Growth of both yeasts was inhibited by 0.12 M sodium bicarbonate when incubation was at 4°C; 0.24 M sodium bicarbonate caused a slow die off of yeast. At 18°C, H. wingei became more sensitive and died in the presence of 0.12 M sodium bicarbonate, but S. cerevisae became resistant to 0.24 M sodium bicarbonate. These results could not be attributed to bicarbonate-induced pH elevation or sodium. Potassium and ammonium bicarbonate were also inhibitory, implicating bicarbonate ion as the antimicrobial agent.