Biomaterial-associated infection for Ti–Mo alloy needs to be solved imminently, a functionally-graded porous Ti–15Mo-xCu alloys (x = 3, 7, 11, and 15 wt %) were developed by powder metallurgy. The effects of the Cu content on corrosion resistance, antibacterial properties, and cytocompatibility of the Ti–15Mo-xCu alloys were investigated. The results show the Ti–15Mo-xCu alloys were composed of β-Ti, α-Ti, and Ti2Cu phases. With the addition of Cu element, the Ti2Cu phase of the Ti–15Mo-xCu alloys mainly distributed at the grain boundaries, generating a micro-galvanic cell effect, which led to a decrease in the corrosion resistance of the Ti–15Mo-xCu alloys, compared with the Ti–15Mo alloy. Stable passivation was induced by the oxide films consisting of TiO2, MoO3, and Cu2O on the surface of the alloy during the corrosion process. The antibacterial properties increased with increasing Cu content. The antibacterial rate of the Ti–15Mo-xCu alloy with 7–15 wt % Cu content was greater than 90% against Escherichia coli, revealing good antibacterial properties. Moreover, the biocompatibility of the Ti–Mo–Cu alloy exhibited a downward trend after the culturing when the Cu content ranged from 11 to 15 wt %. Overall, the Ti–15Mo–7Cu alloy exhibited good corrosion resistance, antibacterial properties, and cytocompatibility, revealing its great potential in clinical applications.