This research analyses 24 years of oil extraction in blocks 16 and 67 of the Yasuní National Park (YNP) in the Amazonian Forest of Ecuador, one of the most biodiverse spaces in the world and with the current presence of ancient indigenous communities. As a novel contribution, we have carried out a Life-Cycle Assessment (LCA) that quantifies the footprints associated with the extraction, transportation, refining, distribution and final uses of the oil in four different scenarios (oil for asphalt use, electricity, marine fuel and passenger car transport). This study also sheds light on the energy return at the point of use of different oil-derivatives, and complements this with a qualitative analysis of the social, cultural and environmental implications for the Waorani communities. We conclude that the environmental burdens of the extraction process in blocks 16 and 67 in 2015 were greater than those of countries such as the United States, Saudi Arabia and Indonesia, based on the analysis of 11 impact categories. The blocks' operation is the most unfavourable for the categories of Terrestrial Acidification Potential (TAP), Global Warming Potential (GWP), Terrestrial Ecotoxicity Potential (TEP) and Ecosystem Quality Loss Potential (EQL), with increments of 804.15 %, 105.36 %, 506.29 % and 210.73 %, respectively, in relation to the average of the rest of the extraction systems analysed. Specifically, the present case study shows 75.18 % higher impacts in the blocks addressed, when compared to the Ecuadorian average. During the period 1999-2022, the carbon emissions associated with the oil extraction in these blocks have increased by 139.01%. It has been detected a neo-colonial economic behaviour: while the Ecuadorian state received 21% of the sales, the Spanish government and the oil companies received, on average, 38% and 41% of the per-litre average fuel price, respectively. Thus, 79% of the income stayed in the Global North. We conclude that, on average, 19.64 % of the impacts associated with crude oil production and consumption occur in the Amazonian region of the YNP, depending on the fuel used and the consumption mechanism. For the Global Warming Potential (GWP) impact category, the extraction process carries, on average, 34.51 % of the weight in all of the life-cycle impacts, depending on the consumption scenario. It was also estimated that to be able to use 0.33 kWh of electricity from fuel combustion, 0.47 kWh of energy for goods transport and 0.20 kWh for passenger transport, an investment of 1 kWh is required, with an average extended EROI of 1:3.33. According to the qualitative analysis performed, it has been concluded that the main local impacts are related to the obstacles in environmental monitoring and information, the economic dependence of the communities on the oil extraction company, and cultural transformations; impacts that are not easily quantifiable or detectable using other methodologies. The combination of the qualitative analysis and LCA showed that the neo-colonial economic distribution did not compensate the social and environmental impacts of the oil extraction occurred in the YNP.