Weed infestation is among the main biotic factors affecting growth and yield of various crops. This issue is more drastic for cereal crops like wheat which is the staple food crop of over 2.5 billion population of the world. One the control strategy is the investigated the distribution pattern of weeds under field conditions. In this regard, a survey study was conducted to investigate the distribution pattern of weed species in wheat crop during 2016-18 in district Khanewal, Punjab Pakistan. Thirty-six weed species distributed among fifteen different families collected from the study area. Family Poaceae was dominant with 10 species while family Asteraceae was the second most dominant family with four weed species. Cynadon dactylon was the most frequently found specie with frequency value 79.1% while Paspalum distichum had the highest importance value index (6.96) among the weeds of the study area.