Pasak Bumi Root Research Articles

Unveiling the Anticancer Potential of Pasak Bumi (Eurycoma Longifolia Jack) Root Extract in Prostate Cancer Treatment.

Prostate cancer remains a significant global health concern, necessitating the exploration of novel therapeutic avenues to enhance treatment efficacy and mitigate adverse effects. Objective This study delves into the potential anticancer properties of Pasak Bumi (Eurycoma longifolia Jack) root extract, a traditional Southeast Asian medicinal plant, against prostate cancer. The research employs a multifaceted approach, encompassing molecular and cellular analyses to unravel the intricate mechanisms underlying Pasak Bumi's effects on prostate cancer cells. Primary focus is given to the PTEN/P13k/Akt pathway, a critical regulator of cell survival and apoptosis. Various concentrations of Pasak Bumi root extract are applied to prostate cancer cell lines, and the impact on apoptosis, cell proliferation, and key molecular targets is assessed. Preliminary findings reveal that Pasak Bumi root extract induces apoptosis in prostate cancer cells, evidenced by downstream molecular events associated with programmed cell death. The extract demonstrates concentration-dependent effects, with higher concentrations exhibiting more pronounced anticancer activity. Moreover, Pasak Bumi root extract appears to modulate the PTEN/P13k/Akt pathway, providing a potential mechanistic link to its anticancer effects. Discussion: The study's significance lies in its contribution to the evolving landscape of natural compounds as anticancer agents, particularly in the context of prostate cancer. Pasak Bumi's traditional use as a medicinal plant, coupled with emerging scientific evidence, underscores its potential translational value. The observed modulation of the PTEN/P13k/Akt pathway aligns with the current understanding of prostate cancer pathogenesis, offering a plausible explanation for Pasak Bumi's anticancer effects. This research sheds light on the promising anticancer potential of Pasak Bumi root extract against prostate cancer. Further exploration of its molecular interactions, synergy with conventional therapies, and efficacy at different stages of cancer progression is warranted. The findings present Pasak Bumi as a nature-inspired candidate for prostate cancer treatment, warranting continued investigation into its therapeutic applications. As the scientific community endeavors to enhance cancer treatment modalities, Pasak Bumi emerges as a captivating subject in the pursuit of effective and minimally invasive prostate cancer therapies.

Construction of biology magazine based on the antidiabetic test of pasak bumi roots

The enrichment program is an additional effort or activity given to students who have achieved complete learning with the aim of increasing students' understanding and insight into the material being or has been studied. In carrying out the enrichment program, teachers can use enrichment materials as learning resources, one of which is a biology magazine. This study aims to develop a biology magazine as an enrichment material to increase students' understanding and insight into the hormone system sub-material. This type of research is an R&D research that uses an adaptation of the 4D Thiagarajan model, namely define, design, and development. The data sources for this research consisted of 36 students from Christian High School Immanuel Pontianak and SMA Santo Fransiskus Asisi Pontianak, 3 biology teachers from these schools, and 3 lecturers of Biology Education FKIP UNTAN. The results of the analysis of student needs indicate that it is necessary to develop a biology magazine in order to motivate students to increase their understanding and insight. The results of the feasibility test can be seen that biological magazines are suitable for use as material for enrichment of the hormone system sub-material, this is indicated by the Aiken's V value obtained 0.79.

POTENTIAL OF PASAK BUMI (E. LONGIFOLIA JACK) ROOT AS AN ANTICANCER AGENT FOR PROSTATE ADENOCARCINOMA CELLS PC3

The article describes the potential of Pasak Bumi root as an anticancer agent for the prostate by inhibiting proliferation of PC3 cells. Using the roots of Pasak Bumi E. longifolia extracted with ethanol solvent. Prostate cancer PC-3 cell culture was obtained from independent androgen prostate adenocarcinoma that had bone metastases as the research subject. This study was an in vitro experimental study with a post-test control group design. Analysis of PC3 cell viability used the MTT assay method. ANOVA test results showed that p-value (Sig.) at 0.011 and smaller than α = 0.05. Therefore, it can be concluded that there is a significant difference in the viability of adenocarcinoma cells in the administration of ethanol extract Pasak Bumi root with different concentrations. Our research showed the result that ethanol extract of Pasak Bumi root has the ability to inhibit the proliferation of PC-3 prostate cancer cells. The higher the ethanol extract concentration of the Pasak Bumi root, the lower the viability of PC-3 cells. Our research proposed the ethanol extract of Pasak Bumi root can be used as an anticancer agent for the prostate through a proliferation inhibition mechanism.

Anticancer Activity of Pasak Bumi Root Extract (Eurycoma longifolia Jack) on Raji Cells.

<b>Background and Objective:</b> The use of the roots of the pasak bumi (<i>E. longifolia</i> Jack) to treat cancer has been studied widely, however, the scientific basis of these plants used as an anticancer drug is widely unknown. The purpose of this study was to examine the anticancer activity of ethyl acetate and non-ethyl acetate fractions of pasak bumi roots in Raji cells. <b>Materials and Methods:</b> The cytotoxicity test is using the direct cell count method with trypan blue staining. The growth inhibition is using doubling time analysis of Raji cells. Observation of the apoptotic events of Raji cells used ethidium bromide staining, while observing the expression of p53 protein in Raji cells was done by immunohistochemical staining. <b>Results:</b> The results of the cytotoxicity and doubling time test showed that the activity of the non-ethyl acetate fraction was greater than that of the roots of pasak bumi. The lower concentration of non-ethyl acetate fraction of pasak bumi roots was able to delay the multiplication time of Raji cells which was greater than that of ethyl acetate. The results of the cytotoxicity and doubling time test showed that the activity of the non-ethyl acetate fraction was greater than that of the roots of pasak bumi. <b>Conclusion:</b> It can be concluded that the ethyl acetate and non-ethyl acetate fractions of the roots of pasak bumi have cytotoxic and antiproliferative activity on Raji cells, however they cannot induce apoptosis in Raji cells. The death of Raji cells is through the mechanism of inhibiting Raji cell proliferation as evidenced by an increase in p53 protein expression.

Prediction of the potency of active compounds in pasak bumi root as the treatment for prostate cancer through phosphatase and tensin homolog (PTEN) pathway

Prediction of the potency of active compounds in pasak bumi root as the treatment for prostate cancer through phosphatase and tensin homolog (PTEN) pathway

Effect of Active Compound of Pasak Bumi Root (Euricoma longifolia, Jack) as an inhibitor of CDK2 methylation: In Silico Study

Active compound of Pasak Bumi’s root, a native plant of Indonesia, is used as antitumor by triggering cell apoptosis and reactivating the silence tumor suppressor gene caused by hypermethylation, inhibiting cancer cell proliferation. The aim of this study is to know the role of quassinoid from Pasak Bumi’s root (Eurycoma longifolia Jack) as an inhibitor of CDK2 methylation in silico. This is a descriptive study. CDK2 samples are obtained from Protein Data Bank (RSCB.org) with ID3SWR, samples of natural quassinoid are obtained from PUBCHEM NCBI and controlled by Sunitinib (®Sutent). Autodock Vina program PyRx 0.8 is used to analyze Molecular Docking. The process of analyzing molecular interactions is carried out using the LigandScout V.2.0 program. Visualization process are carried out using LigandScout V.2.0 program. The affinity of quassinoid and sutinab to CDK are -6.1 and -9.4, respectively. The more negative the binding affinity value, the better the ability of the compound (ligand) to bind to the receptor (macromolecules). From this case, Sutinab has better value compared to quassinoid. Target protein analysis using HITPICK shows quassinoid’s target predictor is JUN protein. Protein interaction analysis are obtained, and the compound is using stitch. JUN protein and Sunitib could bind with CDK2. The conclusion of this study is Sutinib has greater affinity compared to quassinoid.

Efek Ekstrak Etanol Akar Pasak Bumi (Eurycoma Longifolia, Jack) terhadap Ekspresi HRAS pada Organ Hati Tikus Galur Sprague Dawley pada Pemberian Doxorubicin

Doxorubicin chemotherapy agents cause serious side effects in normal cells, especially in the liver and heart caused by free radicals. RAS is an important component of the signal transduction pathway that controls cell proliferation, differentiation and survival. The pasak bumi root (Eurycoma longifolia, Jack) has been reported to have acted as an antioxidant and hepatoprotector. This study aims to determine the effect of giving ethanol extract of pasak bumi root on HRAS expression in healthy mice given doxorubicin. This research was conducted by dividing the test animals into 7 groups, namely doxorubicin control group 4.67 mg / kgBB, group II control ethanol extract of pasak bumi roots 200 mg / kgBB, group III, IV and V were the treatment groups given doxorubicin 4.67 mg / kgBW with ethanol extract of the pasak bumi root, each 50 mg / kgBB; 100 mg / kgBB; 200 mg / kgBW, group VI solvent control and group VII healthy control. The study was continued by painting using an immunohistochemical method to see the expression of HRAS in each experimental group. Data analysis was performed by calculating per cent expressions, then proceed with statistical tests using SPSS. The results of statistical tests showed that there were significant differences between the doxorubicin control group (18.56 � 1.85) and the treatment of group 5 (10.43 � 1.71) and there were no significant differences between the doxorubicin control group (18.56 � 1, 85) with group 3 treatment (17.80 � 4.23) and group 4 treatment (16.70 � 1.97). From the results of the study concluded that the ethanol extract of pasak bumi roots at a dose of 200 mg / KgBB can reduce the expression of HRAS in the administration of doxorubicin

The Effect of Pasak Bumi (Eurycoma longifolia, Jack) Roots Ethanol Extract against Hematology Profile of Healthy Volunteers

The roots of Eurycoma longifolia, Jack has a lot of beneficial activity on human health. The use of Eurycoma longifolia, Jack roots as a traditional medicine should be made in the form of a dosage that is more effective, safe, and had no side effects, especially on hematology. Easy preparations are capsule. This study aimed at identifying the effect of ethanol extract capsule of Eurycoma longifolia, Jack roots on hematology of healthy volunteers. Healthy volunteers were assigned in the study, each of which were 10 male and 10 female who met the inclusion criteria. Both groups were given ethanol extract capsule of Eurycoma longifolia, Jack roots for 14 days, with the dose of 300 mg extract once a day, after meal, at night. Hematology examination was performed on day 0; 14 and 42. Results were compared statistically using Repeated ANOVA and Friedman tests. The result of the study indicated that the average value of hematology at the examination day of 0; 14; and 42, there were significant differences in MCV and leukocyte parameters in healthy male volunteers (p<0.05). MCH, LED and monocyte parameters in healthy female volunteers had a significant difference of (p<0.05). However, the parameter values were still within the normal range. Monitoring results showed that some of the side effects were frequent urinary, dizziness, nausea, sweating, insomnia, and increasing appetite on healthy male volunteers. Meanwhile, on healthy female volunteers, there were frequent urinary, insomnia, constipation. Having ethanol extract capsule of the Eurycoma longifolia, Jack roots did not affect the hematology parameters of healthy male and female volunteers, but it caused some side effects.

INFLUENCE OF THE COMBINATION OF PASAK BUMI ROOT AND PROPOLIS EXTRACTS ON PARASITEMIA LEVELS IN MICE INFECTED WITH PLASMODIUM BERGHEI

Objective: Malaria infection remains a global concern due to increasing resistance to artemisinin-based combination therapy. This study examinedthe antimalarial effects of propolis extract alone and in combination with pasak bumi root extract.Methods: In the study, 30 mice were divided into six groups including two control groups, two groups of mice treated with propolis aloneat concentrations of 90 and 180 mg/kg body weight (BW), and two combination groups of mice treated with 90 or 180 mg/kg BW propolis incombination with 60 or 75 mg/kg BW pasak bumi, respectively. Plasmodium berghei 2% was injected into each mouse, and blood smears wereprepared after 8 days to assess parasitemia.Results: The results revealed no significant difference in parasitemia levels between the positive control and the two combination groups (p=0.136 and0.289, respectively). However, superior growth inhibition (GI) results were observed in the combination groups (97.97% and 97.83%, respectively)than in the propolis monotherapy groups, whereas better outcomes were observed in the positive control group (98.63% GI) than in the propolismonotherapy groups (23.88% and 51.66%, respectively).Conclusion: These results illustrate that combination therapy is superior to propolis monotherapy in inhibiting parasitemia.

THE EFFECT OF PASAK BUMI ROOTS TOWARDS BLOOD GLUCOSE LEVEL IN GLUCOSE-LOADED MICE

The aim of this research is to evaluate the effect of pasak bumi roots (Eurycoma longifolia Jack) towards the blood glucose level in glucose-loaded mice. The blood glucose-lowering effects were tested using Oral Glucose Tolerance Test (OGTT) method. The mice were given with infusion of pasak bumi roots at the doses of 0.83; 1.67; 3.33 g/kgBW and methanol extract of pasak bumi roots at the doses of 102; 210; 420 mg/kgBW. All treatments were conducted orally, 30 minutes before the administration of glucose (2 g/kgBW). The blood glucose levels were measured at 0 minute before the administration of glucose and at 15, 30, 60, 90, and 120 minutes after the administration of glucose. Blood samples were obtained through the mice tail’s vena lateralis using glucometer. The blood glucose levels result which were obtained at the 0 until 120 minutes were calculated to obtain AUC. AUV values of each treatment group were analyzed statistically. Based on the results of the research, it can be concluded that the methanol extract of pasak bumi roots has blood glucose-lowering effect at the doses of 210 and 420 mg/kgBW, but infusion of pasak bumi roots does not have effects on lowering the blood glucose level in the glucose-loaded mice.

The determination of metal content, microbial contamination and dissolution assessment of the ethanol extract of pasak bumi root

The Eurycoma longifolia is bitter therefore, to address this problem, it is prepared in a capsule form. The capsule dosage form must has the parameters of good quality, thus expected to increase the potential use of biodiversity and the interest of the community in consuming the medicine from the natural materials. The purpose of this research is to get the capsule product of ethanol extract of the Eurycoma longifolia root of quality through the test of total plate value and yeast yeast contamination, the determination of the metal content of Pb, Hg, Cd and the level of flavonoids released in the capsule dissolution test of ethanol extract Eurycoma longifolia root. The result of this research shows that the dissolution efficiency (DE 12 0 ) capability of ethanol extract of Eurycoma longifolia root is 81,04±2,38%. Test of heavy metal content in capsule of of ethanol extract of Eurycoma longifolia root found level of Pb equal to 4,132 ppm and not detected content of Cd and Hg in capsule. This indicates that the capsule meets the requirements set by BPOM RI. Tests for total plate numbers (ALT) and yeast capsule ethanol extract of the E. longifolia root were each obtained data of 70 x 10 2 colonies / g and 4 x 10 2 colonies / g. It also shows that the capsules meet the requirements by BPOM RI.

PENGARUH PEMBERIAN EKSTRAK AKAR PASAK BUMI (EURYCOMA LONGIFORA JACK) TERSTANDAR TERHADAP GAMBARAN HISTOPATOLOGIK TESTIS TIKUS WISTAR

The root of pasak bumi (Eurycoma longifolia, Jack) is one of plant from Indonesia known as aphrodisiac. The study about its standardized extract as aphrodisiac was show increasing libido, testosterone level, FSH and LH on male rats. This study was conducted to evaluate testosterone level and histopathological changes of the testes of male rats of standardized extract of pasak bumi root. There are 50 old male rats which ages 3-4 months old divided to five groups. Group I as negative control was administered aquadestilata. Group II as positive control was administered testosterone (Andriol®). Group III, IV and V ware given standardized extract of pasak bumi root at the dose 50, 100 and 200 mg/kg body weight Respectively. The extract was given orally twice a day for six days and forty nine days and then the testes was taken out on 7th and 50th day. The histopathological of the testes was evaluated using quantitative parameter by the presence of testes weight, number and diameter of Leydig cells. Testosterone level was taken on 7th and 50th day used ELISA method. The results were analyzed using parametric test, one way ANOVA (P<0,05), post hoc Dunnet (2-sided) (P < 0,05). The result of this study shows that the standardized extract of pasak bumi root was not effect on histopathological changes of the testes but it could increased the level of testosterone m at the dose 100 and 200 mg/kg body weight. Key word : Eurycoma longifolia, Jack, aphrodisiac, histopathological testes testosteron

Evaluation of The Effect of Pasak Bumi (Eurycoma longifolia Jack) to Increase Androgen Levels

Abstract—Background:The ability of reproductive disorders in men and experimental male rat is characterized among other things by a decrease in androgen such as testosterone levels. Materials of pasak bumi root (Eurycoma longifolia Jack) increased libido behavior of experimental male rat. Libido associated with higher levels of testosterone, it is therefore necessary to evaluate the effect of pasak bumi on androgen levels such as testosterone. Methods: Male rats at 9.00 am treated controls and pasak bumi, then at 14.00 pm tempted with estrus female rats 10 minutes. Then, both control and pasak bumi blood samples were collected from male rat heart on day 1st and day 3rd at 14.15 pm. Measurement of testosterone levels were calculated using radioimmunoassay (RIA). Results: An increase in serum testosterone levels in the blood on day-3rd administration pasak bumi boiled water 18 mg/200 g dose significantly different than the control within day 1st to 3rd. The average levels of testosterone administration control (boiled water of aquades) day 1st = 0.50 ng/ml, day 3rd = 2.46 ng / ml, increased markedly on pasak bumi treatment day 1st = 4.00 ng / ml and day 3 = 9.73 ng / ml, (Duncan test, α=0.05). Conclusions: Testosterone levels can be increased markedly after the consumption of the pasak bumi boiled water for 3 days.Keywords—Pasak bumi, a dose of 18 mg/200 g, testosterone.

Evaluation of The Effect of Pasak Bumi (Eurycoma longifolia Jack) to Increase Androgen Levels

Abstract—Background:The ability of reproductive disorders in men and experimental male rat is characterized among other things by a decrease in androgen such as testosterone levels. Materials of pasak bumi root (Eurycoma longifolia Jack) increased libido behavior of experimental male rat. Libido associated with higher levels of testosterone, it is therefore necessary to evaluate the effect of pasak bumi on androgen levels such as testosterone. Methods: Male rats at 9.00 am treated controls and pasak bumi, then at 14.00 pm tempted with estrus female rats 10 minutes. Then, both control and pasak bumi blood samples were collected from male rat heart on day 1st and day 3rd at 14.15 pm. Measurement of testosterone levels were calculated using radioimmunoassay (RIA). Results: An increase in serum testosterone levels in the blood on day-3rd administration pasak bumi boiled water 18 mg/200 g dose significantly different than the control within day 1st to 3rd. The average levels of testosterone administration control (boiled water of aquades) day 1st = 0.50 ng/ml, day 3rd = 2.46 ng / ml, increased markedly on pasak bumi treatment day 1st = 4.00 ng / ml and day 3 = 9.73 ng / ml, (Duncan test, α=0.05). Conclusions: Testosterone levels can be increased markedly after the consumption of the pasak bumi boiled water for 3 days.Keywords—Pasak bumi, a dose of 18 mg/200 g, testosterone.

PENGARUH PEMBERIAN KOMBINASI FRAKSI ETIL ASETAT AKAR PASAK BUMI DAN DOXORUBICIN TERHADAP BERAT BADAN DAN JUMLAN NODUL TIKUS SPRAGUE DAWLEY BETINA YANG DIINDUKSI 7,12 DIMETILBENZ(α) ANTRASEN (DMBA)

Pasak Bumi roots contain quasinoid compounds that have carcinogenesis inhibitory, imunomudulator, antiulcer, and antimalaria effects. This study aims to determine the effect of a combination of ethyl acetate fraction of Pasak Bumi roots and doxorubicin on DMBA-induced white mice by weight and palpation observation. The mice were divided into 8 groups. Each group consisted of 7 female Sprague Dawley mice. Group 1 (normal) was only given food and drink, Group 2 was given fraction of ethyl acetate of Pasak Bumi roots (100 mg/kg body weight, Group 3 (positive control) was given doxorubicin (1.17 mg/kg body weight) 1 times a week for 5 weeks, Group 4 was given DMBA (20 mg/kg) two times a week for 5 weeks, Groups 5 was given DMBA (20 mg/kg) + doxorubicin (1.17 mg/kg), Group 6 was given DMBA (20 mg / kg) + ethyl acetate fractions of Pasak Bumi roots (100 mg / kg), Group 7 was given DMBA (20 mg / kg) + doxorubicin (1.17 mg / kg) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg) and Group 8 doxorubicin ( 1.17 mg / kg body weight) + ethyl acetate fraction of Pasak Bumi roots (100 mg / kg). Observations were made on the basis of body weight of mice and tumor incidence by palpation at week 6 of treatment. The result of this research shows that the administration of ethyl acetate fraction of Pasak Bumi roots and doxorubicin combination can increase body weight of the mice and decrease the number of tumors compared to single doxorubicin.

EFEK FRAKSI ETIL ASETAT EKSTRAK ETANOL AKAR PASAK BUMI (Eurycoma longifolia, Jack) TERHADAP AKTIVITAS FAGOSITOSIS MAKROFAG SECARA IN VITRO

Eurycoma longifolia has been used as a remedy for detoxification, aphrodisiaca, anticancer and immunistimulant. The quassinoids of pasak bumi root extract, have antioxidant activity and immunomodulatory activity by increasing IL-12. This study aims to determine the effect of the ethyl acetate fraction of ethanolic extract of pasak bumi root on macrophages phagocytic activity in vitro. Macrophages were isolated from peritoneal fluid of male Balb/Cmice, and thenwere cultured for 24 hours in 24 well plate. The ethyl actetate fraction ethanolic extract of pasak bumi root with concentration of 10, 50, and 100 µg/ml, lipopolysaccharides and dimethyl sulfoxide were added, and incubated for 1.5 hours. The phagocytosis tests was carried out by adding latex with density 5x104 /well and incubated for 1.5 hours at incubator (5% CO2,37o C). Macrophage were then stained by Giemsa. The results showed that the percentage of active phagocytic cells treated with ethyl actetate fraction of ethanolic extract of pasak bumi root of 10, 50 and 100 µg/ml, lipopolysaccharides, and dimethyl sulfoxide were 95.4%, 90%, 85.4%, 83.4%, and 79% respectively. Phagocytic capacity at treatment levels of 10, 50, and 100 µg/ml, lipopolysaccharides,and dimethyl sulfoxide were 469; 439.8; 360.8; 204.6; and 147.6 respectively. The result showed thatreatment of ethyl acetate fraction of ethanolic extract of pasak bumi root can increase the phagocytic activity of macrophages in vitro.

Habitat Preferences, Distribution Pattern, and Root Weight Estimation of Pasak Bumi (Eurycoma longifolia Jack.)

Pasak bumi (Eurycoma longifolia Jack) is one of non timber forest products with “indeterminate” conservation status and commercially traded in West Kalimantan. The research objective was to determine the potential of pasak bumi root per hectare and its ecological condition under natural habitat. Root weight of E. longifolia Jack was estimated using simple linear regression and exponential equation with stem diameter and height as independent variables. The results showed that the individual number of the population was 114 with the majority in seedling stage with 71 individuals (62.28%). The distribution was found in clumped pattern. Conditions of the habitat could be described as follows: daily average temperature of 25.6oC, daily average relative humidity of 73.6%, light intensity of 0.9 klx, and red-yellow podsolic soil with texture ranged from clay to sandy clay. The selected estimator model for E. longifolia Jack root weight used exponential equation with stem height as independent variable using the equation of Y= 21.99T0,010 and determination coefficient of 0.97. After height variable was added, the potential of E. longifolia Jack minimum root weight that could be harvested per hectare was 0.33 kg.

Lymphocyte Proliferation Activity MTT -Test of Ethanolic Extract of Pasak Bumi Root (Eurycoma longifolia Jack) On Induced 7,12-Dimethylbenz[A] Antracene (DMBA) Female Sprague Dawley Rat

Pasak bumi root (Eurycoma longifolia Jack) contains quasinoid. Quasinoid potentially has antitumor/anticancer, anti-parasitic, and immunostimulatory activity. The aim of this research was to study the lymphocyte proliferation activity MTT-test of ethanol extract of Eurycoma longifolia Jack root on induced by 7,12-dimethylbenz[a] antracene (DMBA) female Sprague Dawley rat. The test was done at 6 groups of 10 SD rat each. Each groups was administered orally with DMBA (Group I) dose of 20 mg/kg bw, corn oil (Group II), baseline (Group III), and ethanol extract of pasak bumi root dose 12.6; 25.2; and 50.4 mg/kg bw (Group IV,V,and VI). Ethanol extract is given for 5 weeks. At the second week five rats were sacrificed to isolate the lymphocytes of spleen then rats are injected orally with DMBA 2 times a week for 5 weeks. At the eight until twenty fourth week all groups were just administered by aquades. Measurement of spleen relative weight and isolation of the lymphocyte spleen were done at second week and the last of experiment. Lymphocyte proliferation activity were measured by MTT-reduction method. Data were analyzed statistically by analyzes of variance (anova) continued by LSD test and Kruskall Wallis continued by Mann Whitney test. The result showed that limphocyte proliferation activity MTT of ethanol extract of E. longifolia root doses of 12.6; 25.2; and 50.4 mg/kg bw for 14 days prior to DMBA-induced are 339.35 ± 8.43;61.20 ± 6.27;310.28 ± 6.81 and dose 50,4 mg/kg bw after DMBA-induced is 122.108 ± 11.426 .Keyword: E. longifolia root, spleen, lymphocyte cell, 7,12-Dimetilbenz (a) antracene (DMBA)

The influence of Pasak Bumi (Eurycoma longifolia Jack.) root infusa to male white mouse libido

Pasak Bumi ( Eurycoma Longifolia Jack.) is one of forest plant which have been used as aphrodisiac for many generation by the people in hinterland Kalimantan. The study was aimed to gain information of influence of pasak bumi root infusa to male white mouse libido. The research was pure experimental applying completely randomized design. Sixteen male white mice was divided into 2 groups which first group (negative control) was treated with aquadest by oral and the second group treated with pasak bumi roots infusa which its dosage 100 mg / 100 g body weight of male white mice by oral once a day for 21 days. .Data of introduction, climbing, and coitus analysed with one way ANOVA and General Linear Measure model with 95% confidence level. The result indicated that usage period of pasak bumi root infusa (Eurycoma Longifolia Jack.) improving male white mice Iibido, which was showed by the increament amount of introduction and climbing of male white mice to female white mice. Key words: Eurycoma longifolia roots, libido, introduction, climbing, coitus