Parts Of Artemisia Vulgaris Research Articles

In vitro Allelopathic Effects of Plant Extracts on Germination of Vicia fabia (partim) Seeds

The experiment evaluated the effect of plant extracts on germination and contamination of Vicia fabia seed “Bobas” variety of horse beans. The materials used for the studies were aqueous extracts made of 40 plant species. The studies aimed to indicate those plant species, which extracts limited their contamination, simultaneously having a stimulating impact on seeds germination. The germination capability of seeds stimulated extracts from green parts of Satureja hortensis, Zea mays stigmata, Verbascum thapsiforme flowers. The number of seeds colonised by microorganisms was limited by extracts from Zea mays stigmata, Taraxacum officinale roots, Urtica dioica leaves. The analysis of the germination capability of seeds and their contamination by germs showed that extracts prepared in the form of brew had the most favourable effect. All forms of extracts from green parts of Artemisia vulgaris,Taraxacum officinale roots,Urtica dioica leaves had the most favourable effect on germination of horse bean seeds and at the same time on limitation of their contamination.

Efficacy of Some Plant Extracts on the Biological Aspects of the Two Spotted Spider Mite Tetranychus urticae Koch (Acari: Prostigmata: Tetranychidae)

Our local traditional herbal and medical plant may be interduce alternative bio material alter the biology of the red spider mite Tetranychus urticae Koch. The present study was conducted to determine the direct and residual effects of the two natural plant extracts, aerial parts of Artemisia vulgaris L. and the seeds of Acacia concinna (Willd.) using soxhelt extractor, against the two spotted spider mite, Tetranychus urticae Koch, at the laboratory controlled conditions, 30±2C° and 70±5% RH. Two concentrations, 0.25% & 0.5% were utilized to study its effects on the biological aspects of T. urticae. The results confirmed the effective influence of these extracts on mite life aspects, which altered and prolonged the mite life cycle, and that reduced the number of generation/year. Moreover, the used extracts also achieved of high mortality percentages, reached 65.52% and 51.72% in pre-oviposition period and oviposition period respectively, when treated with the highest concentration of Artemisia extract. Reduction in hatchability achieved 61.11%, 57.11%, 54.11% and 52.11% for Artemisia 0.25% & 0.5% and Acacia 0.25% & 0.5% respectively.

Variability in the Essential Oils from Aerial Parts of Artemisia vulgaris L. Grown in Uttarakhand (India)

The present study aims to determine the variability in the chemical composition of the essential oils from the aerial parts of Artemisia vulgaris L. grown in different locations of Uttarakhand Himalaya. The essential oils were obtained through hydro-distillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). In the oils from two locations (Jhanjhar and Kaditalla), α-thujone was found as the major constituent, ranging from 14.40–21.66 % followed by artemisia ketone (6.77–8.64 %), trans-caryophyllene (6.22–6.94 %) and 1,8-cineole (4.75–5.13 %); however in Chakrata region, artemisia ketone (29.38 %) was determined as a principal constituent followed by p-cymene (7.60 %) and yomogi alcohol (5.48 %), while α-thujone found to be only 3.10 %. Essential oils from all the locations were dominated by oxygenated monoterpenoids (52.04–59.24 %), whereas oxygenated sesquiterpenoids found to be 23.08–24.05 % in Jhanjhar and Kaditalla locations and only 2.44 % in the oil from Chakrata.

Preliminary Studies on the Antibacterial and Antioxidative Potentials of Hydroalcoholic Extract from the Whole Parts of Artemisia vulgaris L.

A B S T R A C T The present study assessed the antibacterial, cytotoxicity and antioxidative capacity of hydroalcoholic extract from the whole parts of Artemisia vulgaris (AV) using various in vitro assay models. Lipid Peroxidation (LPO), Protein Glycation (PG), Xanthine Oxidases (XO) and the stable 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) radical scavenging assays were used to evaluate the antioxidative capacity of AV extract. The Total Phenolic Content (TPC) was estimated based on Folic Ciocalteau reagent whilst, the phenolic compounds were characterized by HPLC- DAD. Here, the cytotoxicity nature of AV extract was evaluated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reagent on HepG2 hepatocarcinomal cells. Furthermore, the antibacterial potential was determined by using agar dilution method against our panel of bacterial strains. A mild antibacterial activity was exhibited against P. vulgaris ATCC 6830, E. faecalis ATCC 29212, S. mercescens ATCC 9986, S. aureus OK1 and OK3 having MIC value of 0.4 mg mLG 1 but less active towards other test bacteria. Similarly, the AV extract demonstrated a moderate inhibition of DPPH* (IC50: 0.976 µg mLG 1 ), LPO (IC50: 360 µg mLG 1 ), XO (IC50: 112.51 µg mLG 1 ) and PG (IC50: 968.4 µg mLG 1 ). The TPC was found to be 28.04±0.05 mg GAE gG 1 , which corresponds to the HPLC fingerprint. The AV extract exhibited non-toxic effect towards mammalian cells having an IC50 value of 246.32±5.21 µg mLG 1 . The data showed that Artemisia vulgaris had a mild antibacterial and antioxidative capacity despite its low phenolic composition and at least in part support its medicinal uses in folklore medicinal system.

Fumigant toxicity of essential oil of Mugwort (Artemisia vulgaris L.) against three major stored product beetles

Application of plants essential oil for the evaluation of their fumigant toxicity and insecticidal properties is the goal of many researches. In this study, aerial parts of Artemisia vulgaris L. were subjected to hydrodistillation using a Clevenger-type apparatus, and the chemical composition of the volatile oils was studied by gas chromatography–mass spectrometry. Alpha-Pinene (23.56) was the main component of the essential oil. Insecticidal activity of the oil was evaluated against Tribolium castaneum (Herbst), Callosobruchus maculatus (F.) and Rhizopertha dominica (F.) after 24, 48 and 72 h. After 24-h exposure time, C. maculatus was more susceptible (LC50 = 52.47 μl/l air) and T. castaneum was more tolerant (LC50 = 279.86 μl/l air) than other species. LT50 values were indicated using highest concentration of LC50 tests for three species. In general, mortality increased as the doses of essential oil and exposure time increased. These results proposed that A. vulgaris oil might have potential as a control agent against T. castaneum, R. dominica and especially C. maculates in storages.

Evaluation of Analgesic and Anti-inflammatory Activities of Aerial Parts of Artemisia vulgaris L. in Experimental Animal Models

A methanolic extract of aerial parts of Artemisia vulgaris was studied for anti-inflammatory action by carrageenin-induced rat paw edema. The analgesic activity was tested by tail flick method and hot plate method in albino rats and mice. The methanolic extract of Artemisia vulgaris in doses of 200, 400 and 800 mg/ml showed 71.3, 72.2 and 74.00 % inhibition of paw edema respectively at the end of three hour. In the hot plate (hot plate maintained at 55°C) and tail flick model, the methanolic extract of Ar. vulgaris in the above doses increased the pain threshold significantly also administration of A. vulgaris showed dose dependent action in all experimental animal models. The plant had Saponins, flavonoids, Sesquiterpenoids, Oils, Phenols and Tannins. The results of the present study suggest that A. vulgaris has potent analgesic and anti-inflammatory activities.

Essential Oil Composition of the Aerial Parts of Two Artemisia Species (A. vulgaris and A. absinthium) from East Anatolian Region

The aerial parts of Artemisia vulgaris and A. absinthium (Asteraceae) were hydrodistilled to produce the oils in the yields of 0.5 % (v/w) and 0.8 % (v/w) respectively. The oils were analyzed by GC and GC-MS. Thirty five and Thirty one components were identified representing 87.1 %and 90.8 % of the oils, respectively. The main compounds of A. vulgaris were β-thujene (36.0 %), α-thujene (13.6 %), chrysanthenon (5.6 %), piperitone oxide (4.1 %) and caryophyllene oxide (3.1 %) whereas chamazulene (28.6 %) (-) spathulenol (7.4 %), 1-phellandrene (6.1 %), β-calacorene (5.6 %), sabinene (5.5 %), cis-epoxyocimene (4.5 %) and 1H-indene (3.9 %) were the major constituents of A. absinthium. The results discussed with the genus patterns.