Variation Partitioning Research Articles

Pathogenic Fungi Communities in Contrasted Yam Field Soils are Dominated by The Genus Fusarium in Centre Côte d’Ivoire

Below-ground pathogenic fungi of yam, a globally important food crop are shown to be responsible of field and storage diseases and as such are important threats to yam health. However, yam soil pathogenic fungi community composition and soil determinants remain unclear. Here, we explored the effects of soil physicochemical characteristics on soil fungal phytopathogens in contrasted yam fields. Illumina miseq was used to characterize pathogenic fungi communities in yam field soils. In this work, we hypothesise that pathogenic community composition could be linked to specific physicochemical properties within yam contrasted soils. Principal Coordinate Analysis (PCoA) and variance partitioning were used to identify the soil properties that significantly influence pathogenic fungi community compositions within the three sites in a yam production zone. The results have shown that despite the fact that the three sites exhibited contrasted soils grouped in two types according to physicochemical parameters, four ubiquitous genera including Fusarium, Penicillium, Aspergillus and Trichoderma were identified within pathogenic fungi communities in yam field soils, with Fusarium as the most dominant core genus. Soil type determined the distribution of pathogenic fungi communities in yam field soils, and this effect was attributed to soil properties related to yam soil cation exchange capacity (CEC) and silt content as well as three micronutrients including Na, N and total phosphorus.

Riparian structural vegetation types exhibit differential responses to local community drivers

Abstract Riparian vegetation consists of different Structural Vegetation Types (SVTs): woodland, shrubland, and herbaceous communities. Since each SVT may respond differently to abiotic and biotic drivers, our study aimed to disentangle the independent and shared effects of these drivers on distinct SVTs. Using a stratified random sampling approach, we collected 144 plots in a Mediterranean river of central Italy. We applied RDA and variation partitioning to assess the relative importance of local abiotic (lateral gradient, anthropogenic disturbances, environmental factors) and biotic (alien species) drivers on species richness, Shannon diversity index, and community composition of each SVT. After accounting for spatial autocorrelation, abiotic factors emerged as the main filters shaping riparian community assembly. Additionally, our results showed similar responses in the diversity of woodland and herbaceous SVTs to the independent effects of local drivers. In terms of community composition, only woodlands responded to the effects of environment, lateral gradient, and alien species. Consequently, for conservation purposes, different riparian SVTs should be managed separately, as they may respond differently to local drivers. Finally, the shared effects of certain drivers suggest that management measures addressing only a single group of factors are likely less effective.

Abstract WP93: Cross-Platform Proteomics and Machine Learning Algorithms Nominate Biomarkers of Atrial Fibrillation in Stroke Patients: An Exploratory Study

Introduction: Atrial fibrillation (AFib) is a major risk factor for ischemic stroke (IS). AFib diagnosis is critical to optimize secondary prevention and reduce the recurrent stroke risk. Objectives: We undertook an exploratory cross-platform proteomics study to discover plasma biomarkers of AFib diagnosis in patients with IS or transient ischemic attack (TIA). Methods: We used clinical and proteomics data of stroke patients aged ≥18 years lodged within a prospective plasma repository from 2010 to 2014. We collected blood from each patient at hospital admission before administering any therapeutic intervention. Our outcome was differentially expressed levels of proteins in stroke patients with AFib compared to patients without AFib. We performed aptamer-based proteomics using the plasma 7K SomaScan assay. We identified the differentially expressed proteins using (i) ±1.5-fold change and unadjusted p-value <0.05 cut-offs, (ii) Boruta random forest-based machine learning algorithm, and (iii) 30% or more variation explained by AFib in variance partitioning analysis (VPA). We selected the top proteins that were identified using two of the three selection approaches and conducted multivariable adjusted analyses. We conducted internal validation on the same samples using the PeptiQuant Plus biomarker assessment kits (BAK-270) for targeted protein quantitation. Results: We included 60 patients with IS/TIA (mean age 62.9 years, 50% males) classified into 11 AFib and 49 no AFib (Figure 1). SomaScan quantified 7307 protein targets including 6373 unique proteins. We identified 171 differentially expressed proteins in stroke patients with AFib compared to no AFib. After adjusting for age, sex, diabetes, and coronary artery disease in the multivariable analysis, we identified 53 top proteins independently associated with AFib in stroke patients (adjusted p<0.05) (Figure 2). In the validation phase, we quantified 216 proteins using the BAK-270 platform, of which 185 proteins were overlapping with SomaScan. Using BAK-270, we validated increased levels of IGFBP2, B2M, and COL18A1 and decreased levels of CNDP1, AHSG, and SERPINA4 in stroke patients with AFib compared to no AFib (Figure 3). Conclusions: Our exploratory study highlights the potential of plasma proteomics as a valuable tool for discovering protein biomarkers to discriminate IS/TIA patients with AFib compared to no AFib. Further longitudinal studies with adequate sample sizes are needed to support these findings.

Current intensity and hydraulic retention time play differential roles in functional gene expression or electron transfer pathways in a pyrite-filled three-dimensional biofilm electrode reactor (P3DBER).

This study developed a pyrite-filled three-dimensional biofilm electrode reactor (P3DBER) to treat nitrate wastewater with a low carbon/nitrogen ratio. Meanwhile, the joint effect of current intensity (CI) and hydraulic retention time (HRT) on the performance of the P3DBER was investigated. Results indicated that under the optimal conditions (CI = 30 mA, HRT = 4.9 h), the total inorganic nitrogen removal efficiency (TINRE) reached a maximum of 93.5 ± 1.4%, with a low electrical consumption of 0.075 kW·h/g TIN. Increasing CI under different HRTs significantly enhanced the nitrogen removal capacity of the P3DBER. However, at high CI (30 mA), prolonging HRT did not further improve the nitrogen removal efficiency. The introduction of pyrite not only increased the types of electron donors but also could effectively maintain the stability of pH in the P3DBER. Variation partitioning analysis (VPA) showed that CI had a greater impact on the microbial community/functional genes than HRT. In addition, network analysis demonstrated a strong interconnection among microorganisms/functional genes within the P3DBER. This study offers valuable information for optimizing the operating parameters of the P3DBER.

Accelerated stochastic processes of plankton community assembly due to tidal restriction by seawall construction in the Yangtze River Estuary.

Seawall construction has complex ecological impacts. However, the ecological mechanisms within plankton communities under tidal restriction resulting from seawall construction remain unexplored. Using environmental DNA (eDNA) metabarcoding, this study examined the impact of seawall construction on the assembly process of planktonic eukaryote and bacteria communities from the unrestricted area and the tide-restricted area in the Chongming Dongtan Nature Reserve of Yangtze River Estuary. While environmental heterogeneity did not exert a significant influence on alpha diversity of plankton, it had a significant impact on community structure. Variation partitioning analysis (VPA) and neutral community model indicated that neither environmental nor spatial factors were predominant drivers of plankton community composition and structure, instead, they were influenced by stochastic processes. Moreover, it was observed that the relative significance of stochastic processes in the tide-restricted area exceeded that in the unrestricted area. High habitat uniformity and water connectivity resulting from seawall construction may facilitate homogenization and spread among high-abundance groups. The results have significant implications for understanding the mechanisms underlying succession and composition, and for improving ecological assessment and remediation efforts in areas impacted by tidal restriction.

Dissolved phosphorous through dry-wet-dry transitions in a small-dammed river basin: integrated understanding on transport patterns, export controls, and fate.

An integrated understanding of dissolved phosphorous (DP) export mechanism and controls on export over dry and wet periods is crucial for riverine ecological restorations in dammed river basins considering its high bioavailability and retention rates at dams. Riverine DP transport patterns (composition, sources, and transport pathways), export controls, and fate were investigated over the 2020 wet season (5 events) and dry seasons before and after it (2 events: dry(before) and dry(after)) in a semi-arid, small-dammed watershed to comprehend the links between terrestrial DP sources and aquatic DP sinks. Close spatiotemporal monitoring of the full range of phosphorous and total suspended solids (TSSs) and subsequent analyses (hysteresis, hierarchical partitioning, and coefficient of variation) provided the basis for the study. Total-DP (TDP) shared 13-39% (25%) of total-P (TP) through storms, dissolved organic-P (DOP) shared 6-21% (12%), and phosphate-P shared (PO4-P) 7-22% (13%). DP forms displayed strong connections with discharge trends across the wet season, and marked changes in the shares were reported over dry-wet and wet-dry transitions. The DOP fraction of TDP increased from 4% in dry(before) baseflow to 64% at the end of the wet season. The DOP flux increment in stormflow was 20 folds compared to dry(before) baseflow, while that of PO4-P was 2 folds. DOP displayed the least spatial source heterogeneity with minimum anthropogenic pressure on inherent fluxes. DOP originated from overland and near-stream soil sources and was transported via surface runoff and soil water runoff, respectively. Across the wet season, the attrition of overland DOP sources and activation of near-stream soil DOP sources through strengthened hydrological connectivity governed the seasonal DOP trends. Surface and groundwater runoff pathways were important for PO4-P delivery during stormflow; nonetheless, wastewater treatment plant (WWTP) effluent was the main PO4-P source under both baseflow and stormflow regimes, followed by near-stream traditional agriculture lands. The interaction patterns of small dam systems with DP inputs through dry-wet periods were explained. The riverine PO4-P fluxes were profoundly impacted by in-stream biogeochemical and physical processes and small dam systems, while riverine DOP fluxes were relatively less influenced.

Secrets of the Goo: the Genome assembly of the Pacific Banana Slug, Ariolimax columbianus.

The Pacific banana slug, Ariolimax columbianus, is endemic to the forests of the Pacific Northern West. Found throughout coastal foothills and mountains of California, the hermaphroditic molluscs Ariolimax spp. are niche-constrained, hyper-localized, and phenotypically diverse. The evolutionary history and more recent population history and environmental conditions leading to their phenotypic and genetic variation are not understood. To facilitate such research, we present the first high-quality de novo genome assembly of A. columbianus as part of the California Conservation Genomics Project (CCGP). Pacific Biosciences HiFi long reads and Omni-C chromatin-proximity sequencing technologies were used to produce a de novo genome assembly, consistent with the standard CCGP genome assembly protocol. This assembly comprises 401 scaffolds spanning 2.29 Gb, represented by a scaffold N50 of 94.9Mb, a contig N50 of 3.7Mb, and a benchmarking universal single-copy ortholog (BUSCO) completeness score of 93.9%. Future work will use the A. columbianus genome to study the population structure of Ariolimax spp. across California to understand patterns of population structure, genetic diversity and the broader ecological connections with their habitat. This data will contribute to the CCGP, expanding the knowledge about the partitioning of genomic variation across the different ecoregions of California.

Response of plant life forms and soil physical properties to near-natural restoration measures in alpine grassland, Tibetan plateau.

Near-natural restoration measures enhance the stability of plant life forms in degraded grasslands, facilitating the natural succession of plant communities. In this study, we investigated the effects of three natural restoration measures on the alpine meadows of the northeastern Tibetan Plateau: banned grazing (BG), rest grazing (RG), traditional grazing (TG), and continuous grazing (CG). We utilized redundancy analysis (RDA), variation partitioning(VP), hierarchical partitioning (HP), and partial least squares pathway modeling (PLS-PM) to dissect the quantitative relationships between the distribution of plant life forms and soil physical properties under these restoration measures. The results indicated the following: 1) Under each restoration measure, the distribution of life form plants were predominantly characterized by the highest number of hemicryptophytes, followed by geophytes, with the least number of therophytes. We found that the BG treatment had the highest hemicryptophyte height, coverage, aboveground biomass, and importance value, while the CG treatment had the lowest. 2) After near-natural restoration, the soil bulk density (BD) was decreased. The soil moisture characteristics (MC) were increased including soil saturated water content(SSWC), capillary water holding capacity (CWHC), field water capacity (FWC). And capillary porosity (CP) and non-capillary porosity (NP) were increased. 3) VP analysis revealed that MC, BD, and CP together explained 57.4% of the variation in plant life forms communities. 4) The hemicryptophytes benefited from restoration measures and increased CP. In contrast, the decrease in BD negatively affected geophytes. In summary, restoration measures reduce BD by enhancing MC and increasing CP, which affects the distribution of plant life forms. This finding reveals the important role of soil physical properties in plant survival strategies during alpine meadow restoration.

Longitudinal and temporal variation in fish assemblage structure along lowland prairie Neotropical streams and relationships with environmental variables

The structure of fish assemblages in lotic systems usually varies from headwaters to the mouth, related to geomorphological and physicochemical changes. We evaluated the main processes that structure the fish assemblages on spatial, seasonal, and decadal (2009-2010; 2019-2020) scales and relationships with environmental variables in two low-impacted Neotropical Pampean streams tributaries to the Río de la Plata estuary (RdlP). Environmental variables related to the longitudinal variation in the fish assemblage were depth, macrophyte cover, and altitude, showing higher values in the headwater streams compared to downstreams. Species richness increased towards lower stream sectors due to the migration of riverine species from the RdlP. Beta diversity partitioning analysis showed that the greatest proportion of variability was explained by the turnover component in both the longitudinal and seasonal analyses. The headwater streams showed a more stable composition, consisting of species of the order Characiformes associated with aquatic macrophytes, while the downstream streams recorded greater variability due to the entry of migrant Siluriformes from the RdlP, highlighting the essential ecological function of Pampean streams for RldP fish assemblages. The decadal comparison showed that the longitudinal structure is persistent in the long term regardless of the study period. Therefore, the consistent pattern of longitudinal variation in fish assemblages and its explanatory variables have applicability in the conservation and biomonitoring of lowland streams, serving as reference for recovery efforts.

Model‐based variance partitioning for statistical ecology

Model‐based variance partitioning for statistical ecology

Impacts of Future Climate Change and Xiamen’s Territorial Spatial Planning on Carbon Storage and Sequestration

The intensification of climate change and the implementation of territorial spatial planning policies have jointly increased the complexity of future carbon storage changes. However, the impact of territorial spatial planning on carbon storage under future climate change remains unclear. Therefore, this study aims to reveal the potential impacts of future climate change and territorial spatial planning on carbon storage and sequestration, providing decision support for addressing climate change and optimizing territorial spatial planning. We employed the FLUS model, the InVEST model, and the variance partitioning analysis (VPA) method to simulate carbon storage under 15 different scenarios that combine climate change scenarios and territorial spatial planning for Xiamen in 2035, and to quantify the individual and combined impacts of territorial spatial planning and climate change on ecosystem carbon sequestration. The results showed that (1) by 2035, Xiamen’s carbon storage capacity is expected to range from 32.66 × 106 Mg to 33.00 × 106 Mg under various scenarios, reflecting a decrease from 2020 levels; (2) the implementation of territorial spatial planning is conducive to preserving Xiamen’s carbon storage, with the urban development boundary proving to be the most effective; (3) carbon storage is greatly affected by climate change, with RCP 4.5 more effective than RCP 8.5 in maintaining higher levels of carbon storage; and (4) the influence of territorial spatial planning on carbon sequestration consistently exceeds that of climate change, particularly under high-emission scenarios, where the regulatory effect of planning is especially significant.

Effect of Environmental Factors on Macrobenthic Community Structure in Chishui River Basin

Tributaries flowing naturally play an important role in maintaining the biodiversity of aquatic organisms in dammed rivers. The Chishui River is currently the only undeveloped first-level tributary and an important habitat for aquatic organisms in the upper reaches of the Yangtze River. Understanding the distribution of the community structure of macrobenthos in the Chishui River and its influencing factors is crucial for the conservation and restoration of aquatic biodiversity in both the Chishui River and the Yangtze River. This study analyzes the community structure characteristics of macrobenthos in the Chishui River using four indicators, i.e., Margalef richness index, EPT taxon richness (the number of taxa in the pollution-sensitive Ephemeroptera, Plecoptera, and Trichoptera), Simpson dominance index, and Shannon diversity index, examining differences among different types of environmental factors (physical, chemical, and biological) in the upper, middle, and lower reaches. Subsequently, RDA (Redundancy Analysis) is used to analyze the main influencing factors of different types of environmental factors on macrobenthic community structure. VPA (Variance Partitioning Analysis) is employed to assess the relative importance of different types of environmental factors and their joint effects on the characteristics of macrobenthic community structure. The results indicate that physical environmental factors explain 68.7% of the variation in macrobenthic community structure indicators, chemical environmental factors explain 79.3%, and biological environmental factors account for 36.2%. The interaction among chemical, biological, and physical factors is the most significant explanatory variable, accounting for 41.7% of the variation in macrobenthic community structure characteristics. For EPT taxon number and Shannon diversity index, the interaction among chemical, biological, and physical factors is also the most important explanatory variable, accounting for 42.1% and 42.5% of the variation. For the Margalef richness index and Simpson dominance index, the interaction between chemical and physical factors is the most significant, accounting for 45.0% and 85.3% of the variation. Therefore, the impact of multiple environmental factors on aquatic organisms should not be overlooked, and attention should be paid to the contributions of various environmental factors in the conservation of macrobenthos in the Chishui River Basin.

Seasonal Variations in the Structure and Function of the Gut Flora in Adult Male Rhesus Macaques Reared in Outdoor Colonies.

The seasonal variations that occur in the gut microbiota of healthy adult rhesus monkeys kept in outdoor groups under conventional rearing patterns and how these variations are affected by environmental variables are relatively poorly understood. In this study, we collected 120 fecal samples from 30 adult male rhesus monkeys kept in outdoor groups across four seasons and recorded the temperature and humidity of the housing facilities, as well as the proportions of fruit and vegetables in their diet. A 16S rRNA sequencing analysis showed that the alpha diversity of the gut microbiota of the rhesus monkeys was higher in winter and spring than in summer and autumn. A principal coordinate analysis (PCoA) further demonstrated notable seasonal variations in the composition and functionality of the gut microbiota in the rhesus monkeys. The phyla Firmicutes and Bacteroidetes and the genus Prevotella 9 were the significantly dominant groups in all 120 fecal samples from the rhesus monkeys. A linear discriminant analysis (LDA) effect size (LEfSe) analysis (LDA > 4) indicated that at the phylum level, Firmicutes was significantly enriched in winter, Bacteroidetes was significantly enriched in summer, and Proteobacteria and Campylobacter were significantly enriched in spring. At the genus level, Helicobacter and Ralstonia were significantly enriched in spring; Prevotella 9, Streptococcus, and Prevotella were significantly enriched in summer; and UCG_005 was significantly enriched in autumn. The beneficial genera Lactobacillus, Limosilactobacillus, and Ligilactobacillus and the beneficial species Lactobacillus johnsonii, Limosilactobacillus reuteri, Ligilactobacillus murinus, and Lactobacillus amylovorus all showed the same seasonal trend; namely, their average relative abundance was markedly greater during the winter months compared to other seasons. Compared with other seasons, carbohydrate metabolic function was significantly upregulated in winter (p < 0.01), amino acid metabolic function was relatively increased in spring, and energy metabolic function and the metabolic function of cofactors and vitamins were significantly downregulated in winter and relatively upregulated in summer. A variance partitioning analysis (VPA) and redundancy analysis (RDA) showed that the proportions of fruits and vegetables in the diet, but not climatic factors (temperature and humidity), significantly influenced the seasonal changes in the gut microbiota. These variations were related to changes in the proportions of fruits and vegetables. This research presents novel findings regarding the influence of external environmental factors on the gastrointestinal environment of rhesus monkeys.

Associations of Perceived School and Year Group Climate with Mental Health Among Children Aged 7-to-11 Years

Abstract Schools are an important setting for interventions to improve mental health. There is growing evidence that school climate – sometimes expressed as the perceptions that children have about the relationships, safety, values, and beliefs within their school – can impact child mental health. Poor child mental health is associated with feelings of distress as well deficits in functioning. However, while most studies have focused on school climate, climate at lower levels of nesting, including year group, may be important. Cross-sectional data on emotional and behavioural difficulties from 32,606 children in primary schools in Wales (ages 7–11, year groups 3–6) were collected via a school survey, delivered online and within the classroom environment to all children who consented, and analysed using multilevel modelling. Models were then extended to consider how aggregated measures of year group and school climate are associated with mental health outcomes. The unadjusted variance partition coefficients (VPCs) indicated that 2.8% of the variance in emotional difficulties and 3.2% in behavioural difficulties were attributable to differences in the year group, while school-level differences represented 2.4% and 3.5%, respectively. More positive year group and school climate were associated with more positive mental health outcomes. School and year group climate are independently associated with primary school children’s mental health. Interventions to support mental health should aim to optimise the whole school climate, as well as climate within year group clusters.

Immigration reduces selection in water microbial community assembly.

To investigate the influence of immigration on the selection in structuring local water bacterial communities, we conducted a new community assembly experiment using microcosms filled with sterile original water medium under outdoor conditions. We collected air particulate matter from dust pooled from samples collected at 10 locations across ~20 km in a warm temperate region in Linfen City (northern China). The immigration rates were increased by introducing air particulate matter into the microcosms. The diversity, structure, and composition of the bacterial community in the water were assessed using 16S rRNA gene amplicon sequencing on the 13th and 60th days after the start of the experiment. Our results showed that increasing immigration did not lead to significant changes in the overall diversity of the total bacterial community on the 13th day. However, on the 60th day, diversity significantly increased. The variation explained by the environment substantially decreased from the 13th to the 60th day. The amount decreased from the control to the high immigration treatments, with a range of 65.0 to 29.8% on the 13th day and 34.0 to 15.4% on the 60th day. The dominant phyla differed significantly. In the early stage, Proteobacteria (69.6%) accounted for a higher relative average abundance, while Firmicutes (4.6%), Cyanobacteria (6.0%), Planctomycetota (8.1%), Verrucomicrobiota (2.0%), and Halobacterota (0.9%) were more abundant in the late stage. Additionally, the late stage had an average of 33 phyla, compared to 15 phyla in the early stage. All the results suggested a minimal role of dispersal limitation in structuring water bacterial communities in the early stage, whereas, in the late stage, the bacterial communities might experience dispersal swamping in our study area. Variance partitioning indicated that throughout the experiment, increasing immigration weakened the signal of environmental selection in the water microbial community assembly. These results expand our understanding of the impact of immigration on environmental selection and provide insights into the varying importance of dispersal and selection on microbial community assembly at different stages of succession.

Niche Partitioning and Intraspecific Variation of Thaumarchaeota in Deep Ocean Sediments.

Deep-sea sediments contain a large number of Thaumarchaeota that are phylogenetically distinct from their pelagic counterparts. However, their ecology and evolutionary adaptations are not well understood. Metagenomic analyses were conducted on samples from various depths of a 750-cm sediment core collected from the Mariana Trench Challenger Deep. The abundance of Thaumarchaeota and archaeal amoA generally decreased with depth, except for an unexpected peak midway through the core. The thaumarchaeotal metagenome-assembled genomes were classified into diverse phylogenetic clusters associated with amoA-NP-γ, amoA-NP-θ, and amoA-NP-δ of ammonia-oxidising Thaumarchaeota and non-ammonia-oxidising lineages. The most abundant group was within amoA-NP-γ, which is usually found in coastal and shallow habitats, indicating potential niche expansion from marine shallow to hadal environments. This benthic group showed within-species genomic variations compared to the previously identified Hadal water group, suggesting microdiversification of hadal Thaumarchaeota along with niche separation between benthic and pelagic environments. Evolutionary adaptations associated with the benthic-to-pelagic transition included reduced genome size, loss of motility/cell adhesion, altered energy metabolism, and different mechanisms for substrate acquisition and regulation (e.g., ammonium). These findings offer new insights into the evolution of hadal Thaumarchaeota and demonstrate, for the first time, intraspecies-level genomic variation in Thaumarchaeota related to the benthic-versus-pelagic niche partitioning in the deep ocean.

Plan of Care Visits: Implementation During Hospitalization and Association With 30-Day Readmissions in a Large, Integrated Health Care System.

Plan of Care of Visits (POCV), including the patient, nurse, and hospital provider were implemented across an integrated health system to improve provider-patient communication during hospitalization and patient outcomes. To assess POCV adoption after implementation, patient characteristics assosites were classified as teachsites were classified as teachsites were classified as teachsites were classified as ciated with POCV completion, and association of POCV with 30-day readmissions. This retrospective cohort study utilized electronic medical record (EMR) data of 237,430 adult patients discharged to home from 11 hospitals from January 2020 to December 2022. POCV completion was a discrete EMR variable. POCV adoption was estimated monthly by hospital as proportion of patients with at least 1 POCV during hospitalization, with variation among hospitals measured using the Variance Partition Coefficient (VPC). Multivariable logistic regressions assessed factors associated with POCV completion and POCV association with 30-day readmission. POCV adoption increased from 69% to 94% (2020-2022) and varied by 50% across hospitals (VPC 0.50, 95% CI: 0.29-0.70). Odds of a discharge-day POCV were lower among older patients (≥65 vs. 18-34y, OR 0.81, CI: 0.79-0.83), and higher among female (OR 1.06; CI: 1.04-1.07), Asian (vs. White, OR 1.13; CI: 1.06-1.21), Hispanic (OR 1.09; CI: 1.05-1.13), and surgical patients (vs. medical, OR 1.33; CI: 1.30-1.35). Patients completing discharge-day POCV had lower 30-day readmission odds (2022 OR 0.76, CI: 0.73-0.79). Patients with POCV on ≥75% of hospital days had similar readmission odds trends. POCV implementation was successful, and POCV completion was associated with fewer 30-day readmissions. Future work should focus on increasing POCV adoption while reducing hospital variation.

Pulling nutrients from Mo-polluted soil by arbuscular mycorrhizal fungi extraradical mycelia is quenching thirsty with poison.

As an extension of plant root system, arbuscular mycorrhizal fungi (AMF) extraradical mycelium (ERM) can break the limitation of rhizosphere and play an important role in plant nutrient acquisition. However, it remains unclear whether ERM is smart enough to pick out nutrients while avoiding poison, or is unable to pick out nutrients and have to absorb poisons together. Therefore, the present study employed a compartment device to separate the mycelia from roots, aiming to explore the nutrient absorption pathways of mycelia in molybdenum (Mo) pollution soil after inoculation with AMF in maize and vetch plants. The results showed that mycelia increased the absorption of nutrients in maize and vetch plants, while reducing the biomass of the plants. In addition, mycelia increased the Mo concentration of plants. The highest contribution of mycelia to Mo concentration in plants reached 114.06%. For different plants, maize tends to immobilize Mo in roots, while vetch tend to transport Mo to shoots under Mo stress. Additionally, variance partitioning analysis proved that the contribution of nutrients to biomass was the largest, with the interpretation rates reaching 65.09% and 47.26% in maize and vetch plants, respectively. The decrease in plant biomass was due to the toxic effects of Mo. Furthermore, the negative effect of the interaction of Mo, mycelia and nutrients on biomass further proved that the nutrients absorption of mycelia under Mo-polluted soil may be carried out in the way of quenching thirsty with poison.

Linking new national active biomonitoring data with stream macroinvertebrate communities suggests large-scale effects of toxic contamination on freshwater ecosystems.

Since recent years, an increasingly large number of toxic chemicals enters watercourses threatening freshwater biodiversity. But ecological studies still poorly document the quantitative patterns linking exposure to complex mixture of toxic chemicals and species communities' integrity in the field. In this context, French monitoring authorities have recently deployed at a national scale in situ biotests using the feeding inhibition of the crustacean Gammarus as toxicity indicator. In this paper, we conjointly exploit this new type of biomonitoring dataset and ecological data for macroinvertebrates to gain information about the structuring influence of toxicity on aquatic communities. Especially, we used multivariate analyses with variation partitioning for testing the hypothesis that toxicity (feeding inhibition index) can explain variations in the taxonomical composition between 76 stations on French streams while, for different spatial scales, estimating the confounding influences of other environmental and spatial factors. Our results showed that changes in the toxicity indicator were significantly associated with specific changes in the taxonomic composition of stream macroinvertebrate communities. That association was weakly confounded with the effects of environmental and spatial factors, especially at the largest spatial scale considered. That taxon turnover linked to toxicity was associated with reduced richness at the community scale, and the replacement of native taxa by alien taxa. Overall, our study thus supports the hypothesis that toxic contamination modifies the structure of stream communities and ergo threatens aquatic biodiversity.

Deer Browsing Increases Stem Slenderness and Crown Irregularity and Modifies the Effects of Light Gradients on Architecture of Forest Tree Saplings.

Browsing by ungulates is commonly assumed to target the upper parts of sapling crowns, leading to reduced vertical growth or even growth cessation. However, the extent to which browsing induces shifts in resource allocation toward lateral growth remains unclear. This study explores the impact of browsing intensity (BI) and light availability on the architectural traits of six temperate tree species, focusing on height-diameter ratio (H/D), crown slenderness (CL/CW), and crown irregularity (CI) across sapling height classes. Browsing pressure and architectural responses varied across height groups, reflecting diverse adaptive strategies. BI was weakly but negatively correlated with sapling height, indicating that even tall saplings (> 2 m) experience browsing, particularly in the lower crown. H/D consistently increased with BI across all height classes, with stronger effects in medium and tall saplings. Light influenced H/D differently between browsed and unbrowsed saplings: unbrowsed saplings showed reduced H/D only under high light conditions, while browsed saplings exhibited consistent reductions regardless of light levels. CL/CW was negatively but insignificantly affected by BI. Light increased CL/CW in unbrowsed saplings across all height classes but decreased it in browsed short and medium saplings, suggesting a 'pruning' effect of browsing that altered competition dynamics. Species-specific analysis of Fagus sylvatica revealed an increase in CL/CW with BI, reflecting unique adaptive responses. CI increased significantly with BI across all height classes, with the strongest effects in medium and tall saplings. Light reduced CI in browsed short saplings but had inconsistent effects on unbrowsed individuals. Variation partitioning showed that light explained most variation in H/D and CL/CW for shorter saplings, while BI predominantly influenced CI in taller ones. By integrating the effects of browsing and light, this study provides insights into juvenile tree adaptations and resilience under ecological stressors, advancing our understanding of tree growth strategies in challenging environments.