Particulate Organic Matter Fractions Research Articles

Fractionation of Inorganic Phosphorus in Cold Temperate Forest Soils: Associating Mechanisms of Soil Aggregate Protection and Recovery Periods after Forest Fire Disturbance

The soil aggregate is the fundamental unit of soil structure. The fractionation characteristics and influencing factors of phosphorus (P) in soil aggregates inherently link its geochemical characteristics and recycling mechanism. This work investigated the fractionation characteristics of inorganic P in cold temperate forest soils and studied the impacts of recovery periods after forest fires and soil aggregate protection mechanisms on P fractionation. Our results showed that the TP, active P, stable P, and total organic carbon (TOC) contents varied with increasing recovery years after forest fire disturbance. The TP content in the coarse particulate organic matter fraction (cPOM) exhibited an increasing trend with the number of recovery years. Redundancy analysis (RDA) and correlation analysis indicated that TOC played a crucial role in influencing the dynamics of P fractionation during the recovery process. The order of TP levels in different soil aggregate fractions was as follows: μClay > dClay > LF > cPOM > dSilt > μSilt > iPOM, with significant contributions from the cPOM and dSilt fractions. The ranking of P fractions in bulk soils was as follows: ACa-P > Fe-P > Oc-P > Or-P > De-P > Al-P > Ex-P. The protective mechanism of soil aggregates had a more significant effect on TOC than TP, with the order of protective abilities being: Phy×biochem-protected > Biochem-protected > Phy-protected > Non-protected mechanism. TOC and recovery years emerged as critical factors influencing the dynamics of different P fractions during post-fire recovery. Soil aggregate protection mechanisms demonstrated significantly higher effects on TOC than on TP. This study provides insights into the fractionation mechanisms of P in the soil–forest ecosystem of the Greater Khingan Mountains, contributing to the sustainable development and utilization of cold temperate forest ecosystems.

Upper limit of mineral-associated organic carbon in temperate and sub-tropical soils: How far is it?

Soil organic carbon (SOC) is the main terrestrial C pool and is roughly divided into stable mineral-associated organic carbon (MAOC) and particulate organic matter carbon (POC). However, the sequestration capacity of MAOC remains unquantified. Currently, soils are considered to have a limited capacity to accumulate MAOC, primarily due to the presence of fine fractions, i.e. silt and clay particles. However, it is unlikely that saturation will occur in all soils due to land use. Therefore it is necessary to investigate whether soils from different latitudes and their silt and clay fractions that comprise MAOC are saturated. We selected 71 soil samples that were physically fractionated from a Mexican inventory of vegetation with a wide range of SOC (4–186 g kg−1 soil) and clay (126–777 g kg−1 soil) contents. Soils were grouped into non-volcanic soils and allophanic Andosols. In the first group, we found a positive and highly significant linear relationship between SOC and MAOC contents, but no upper limit was detected. We found a common slope of 0.86 (R2 = 0.97, p < 0.01), i.e. 86% of the SOC was contained in MAOC irrespective of soil types and management, a value very similar to that in other studies. Conversely, for allophanic Andosols, which had SOC content twice that of non-volcanic soils, a second-order polynomial function (R2 = 0.98, p < 0.01) was established, the upper limit of which exceeded 186 g SOC kg−1 for a MAOC of 134 g kg−1. This was also supported by an increasing polynomial function of particulate organic carbon (POC) as the SOC increased. In both types of soils, maximum MAOCs were found between 700 and 800 g kg−1 of silt and clay in medium-textured soils. The amount of silt and clay particles was limited by SOC accumulation in the silt fraction as clay became saturated. These results are influenced by the mass proportion of silt and clay that follows a unique pattern in allophanic Andosols and non-volcanic soils. An investigation of a comprehensive database comprising 21,315 soil profiles sampled 0–30 cm across Mexico showed that most soils are below 186 g SOC kg−1 the bending point above which the upper limit does exist.

Management and liming-induced changes in organo-Al/Fe complexes and amorphous mineral-associated organic carbon: Implications for carbon sequestration in volcanic soils

Amorphous mineral-associated organic carbon (AMAOC) and organo-Al/Fe complexes (Cp) play crucial roles in soil carbon (C) sequestration in volcanic soils. This study investigated the effects of land use management and liming on the C sequestration capacity of a young allophanic Andisol and old halloysitic-kaolinitic volcanic Ultisol sampled at 0–10 and 10–20 cm depth. This study was designed to test two hypotheses: (1) management and liming affect the C sequestration capacity of Andisol by reducing Cp and increasing AMAOC to offset Cp losses, and (2) they do not significantly affect the carbon sequestration capacity of Ultisol because of its more crystalline mineral forms and reduced amount of Cp. This study was conducted using a selective dissolution technique and density fractionation with agricultural soils and forests as control soils. After 43 years of intensive management and liming, the soil organic carbon (SOC) content decreased by 32% in the topsoil compared to the original native forest soil (178 ± 2 g kg−1 soil), mainly in the particulate organic matter (POM) fraction, which decreased by 74% compared to the native forest soil (61 ± 9 g kg−1 soil). However, Cp decreased only by 40% compared to the forest soil (62 ± 1 g kg−1 soil) from pHKCl 4.8–5.0, and AMAOC increased in 17% in the topsoil and 64% in the subsoil, supporting the first hypothesis of this study. In the cultivated Ultisol, SOC decreased by 35% due to the POM fraction, and mineral-associated organic C (MAOC) decreased by 37% compared to the forest soil (38 ± 7 g kg−1 soil). These results partially support the second hypothesis because the crystalline clay minerals from which MAOC originated remained unchanged and were strongly correlated with SOC. The mass balance of both soils indicated that the stable C losses from native forest soils were < 14%. These results highlight the importance of different C sequestration mechanisms to compensate for SOC losses in the young allophanic Andisol and old volcanic Ultisol. Our findings imply that both mechanisms can be used to estimate the C sequestration capcity in similar soil types.

Shifts in controls and abundance of particulate and mineral-associated organic matter fractions among subfield yield stability zones

Abstract. Spatiotemporal yield heterogeneity presents a significant challenge to agricultural sustainability efforts and can strain the economic viability of farming operations. Increasing soil organic matter (SOM) has been associated with increased crop productivity, as well as the mitigation of yield variability across time and space. Observations at the regional scale have indicated decreases in yield variability with increasing SOM. However, the mechanisms by which this variability is reduced remain poorly understood, especially at the farm scale. To better understand the relationship between SOM and yield heterogeneity, we examined its distribution between particulate organic matter (POM) and mineral-associated organic matter (MAOM) at the subfield scale within nine farms located in the central United States. We expected that the highest SOM concentrations would be found in stable, high-yielding zones and that the SOM pool in these areas would have a higher proportion of POM relative to other areas in the field. In contrast to our predictions, we found that unstable yield areas had significantly higher SOM than stable yield areas and that there was no significant difference in the relative contribution of POM to total SOM across different yield stability zones. Our results further indicate that MAOM abundance was primarily explained by interactions between crop productivity and edaphic properties such as texture, which varied amongst stability zones. However, we were unable to link POM abundance to soil properties or cropping system characteristics. Instead, we posit that POM dynamics in these systems may be controlled by differences in decomposition patterns between stable and unstable yield zones. Our results show that, at the subfield scale, increasing SOM may not directly confer increased yield stability. Instead, in fields with high spatiotemporal yield heterogeneity, SOM stocks may be determined by interactive effects of topography, weather, and soil characteristics on crop productivity and SOM decomposition. These findings suggest that POM has the potential to be a useful indicator of yield stability, with higher POM stocks in unstable zones, and highlights the need to consider these factors during soil sampling campaigns, especially when attempting to quantify farm-scale soil C stocks.

Enhanced Soil Carbon Stability through Alterations in Components of Particulate and Mineral-Associated Organic Matter in Reclaimed Saline–Alkali Drainage Ditches

Soil carbon content and stability are primarily influenced by the stabilization of particulate organic matter (POM) and mineral-associated organic matter (MAOM). Despite extensive research on the stabilization processes of POM and MAOM carbon components under various land-use types, the investigation into stabilization processes of soil carbon remains limited in saline–alkali soils. Therefore, we collected soil samples from different positions of saline–alkali drainage ditches at four reclamation times (the first, seventh, fifteenth, and thirtieth year) to determine their carbon content and physicochemical properties. Moreover, POM and MAOM fractions were separated from soil samples, and Fourier transform infrared spectra (FTIR) were used to investigate changes in their chemical composition. The results showed that with increasing reclamation time, the soil total carbon and soil organic carbon (SOC) contents significantly increased from 14 to 15 and 2.9 to 5.5 g kg−1, respectively. In contrast, soil inorganic carbon content significantly decreased from 11 to 9.6 g kg−1. Notably, the changes in soil carbon components following the increasing reclamation time were primarily observed in the furrow sole at a depth of 20–40 cm. While the SOC content of the POM fraction (SOCPOM) decreased significantly, the SOC content of the MAOM fraction (SOCMAOM) increased significantly. These alterations were largely dominated by drainage processes after reclamation instead of a possible conversion from SOCPOM to SOCMAOM. FTIR results revealed that MAOM was greatly influenced by the reclamation time more than POM was, but the change in both POM and MAOM contributed to an increase in soil carbon stability. Our findings will deepen the comprehension of soil carbon stabilization processes in saline–alkali drainage ditches after reclamation and offer a research framework to investigate the stability processes of soil carbon components via alterations in POM and MAOM fractions.

Effects of biobased fertilisers on soil physical, chemical and biological indicators – a one-year incubation study

Soil quality is declining in Europe and globally due to agricultural practices and climate change. The European market for novel biobased fertilisers (BBFs) is growing and the new European Union fertiliser regulation promotes their use. However, knowledge about the effects of many novel BBFs on soil quality is currently very limited. In a one-year laboratory incubation experiment, this study aimed to test the effect on biological (microbial biomass carbon (C)), physical (clay dispersibility and water-holding capacity) and chemical (pH, cation exchange capacity (CEC), total C and C in soil size fractions (&lt;250, 50–250 and &gt;50 μm)) soil quality indicators of 10 BBFs applied at two different rates on two soil types: an Arenosol and a Luvisol. The set-up also included a soil that was subjected to long-term annual application of the compost used in the incubation. The application of BBFs generally improved soil quality, with the compost material improving soil quality most, followed by a plant-based fertiliser and a biogas digestate. The effect of BBF application on CEC, total C and particulate organic matter (POM) was related to the amount of total C added with the BBF. Furthermore, the effect on total C and POM fractions was also related to easily decomposable C added with the BBF. Comparing the single accelerated application with annual application under field conditions indicated that the long-term incubation trial is a reasonable predictor of compost long-term effects in the field. Whether this applies to BBFs with very different properties remains to be shown.

Soil carbon and nitrogen after eight years of rotational grazing in the Nebraska Sandhills meadows

Grassland provides many ecosystem services; therefore, sustainable management practices of grassland are crucial for maintaining and enhancing its ecosystem health and resilience. Rotational grazing at a high stocking density (a.k.a., ultrahigh stocking density) is purported to sequester greater amounts of carbon (C) in grassland soils than rotational grazing at low stocking densities. This study was conducted in the Nebraska Sandhills meadows for eight years to evaluate how rotational grazing with different stocking densities can affect soil C and total nitrogen (TN) in bulk soils, soil organic matter fractions, and sequestration rate. The grazing management included a high stocking density with one grazing cycle (MOB), a 4-pasture rotation system with one grazing cycle at a low stocking density (4PR1), and no grazing (CNT). Results showed that soil C and N contents were higher in the total particulate organic matter fraction than in the soil mineral-associated organic fraction at the 0–10 cm depth and visa verse at the 10–20 cm depth. Grazing management did not affect C and N contents in the bulk soils (SOC averaged 28.19 g C kg−1 and TN averaged 2.67 g N kg−1 at the 0–10 cm depth). Both MOB and 4PR1 grazing treatments recorded similar C and N contents in all SOM fractions. However, the MOB grazing treatment increased C and N contents in the macro-aggregate occluded particulate and dissolved organic matter fractions compared to the CNT. The 4PR1 grazing treatment increased soil C and N contents in the dissolved organic matter and mineral-associated organic matter fractions and increased SOC sequestration rate compared to the CNT. Rotational grazing at a low stocking density for one cycle appears to enhance long-term SOC accumulation in these Sandhills meadows soil.

Biochar reduced the mineralization of native and added soil organic carbon: evidence of negative priming and enhanced microbial carbon use efficiency

Biochar has been widely recognized for its potential to increase carbon (C) sequestration and mitigate climate change. This potential is affected by how biochar interacts with native soil organic carbon (SOC) and fresh organic substrates added to soil. However, only a few studies have been conducted to understand this interaction. To fill this knowledge gap, we conducted a 13C-glucose labelling soil incubation for 6 months using fine-textured agricultural soil (Stagnosol) with two different biochar amounts. Biochar addition reduced the mineralization of SOC and 13C-glucose and increased soil microbial biomass carbon (MBC) and microbial carbon use efficiency (CUE). The effects were found to be additive i.e., higher biochar application rate resulted in lower mineralization of SOC and 13C-glucose. Additionally, soil density fractionation after 6 months revealed that most of the added biochar particles were recovered in free particulate organic matter (POM) fraction. Biochar also increased the retention of 13C in free POM fraction, indicating that added 13C-glucose was preserved within the biochar particles. The measurement of 13C from the total amino sugar fraction extracted from the biochar particles suggested that biochar increased the microbial uptake of added 13C-glucose and after they died, the dead microbial residues (necromass) accumulated inside biochar pores. Biochar also increased the proportion of occluded POM, demonstrating that increased soil occlusion following biochar addition reduced SOC mineralization. Overall, the study demonstrates the additional C sequestering potential of biochar by inducing negative priming of native SOC as well as increasing CUE, resulting in the formation and stabilization of microbial necromass.Graphical

The transition between coastal and offshore areas in the North Sea unraveled by suspended particle composition

Identifying the mechanisms that contribute to the variability of suspended particulate matter concentrations in coastal areas is important but difficult, especially due to the complexity of physical and biogeochemical interactions involved. Our study addresses this complexity and investigates changes in the horizontal spread and composition of particles, focusing on cross-coastal gradients in the southern North Sea and the English Channel. A semi-empirical model is applied on in situ data of SPM and its organic fraction to resolve the relationship between organic and inorganic suspended particles. The derived equations are applied onto remote sensing products of SPM concentration, which provide monthly synoptic maps of particulate organic matter concentrations (here, particulate organic nitrogen) at the surface together with their labile and less reactive fractions. Comparing these fractions of particulate organic matter reveals their characteristic features along the coastal-offshore gradient, with an area of increased settling rate for particles generally observed between 5 and 30 km from the coast. We identify this area as the transition zone between coastal and offshore waters with respect to particle dynamics. Presumably, in that area, the turbulence range and particle composition favor particle settling, while hydrodynamic processes tend to transport particles of the seabed back towards the coast. Bathymetry plays an important role in controlling the range of turbulent dissipation energy values in the water column, and we observe that the transition zone in the southern North Sea is generally confined to water depths below 20 m. Seasonal variations in suspended particle dynamics are linked to biological processes enhancing particle flocculation, which do not affect the location of the transition zone. We identify the criteria that allow a transition zone and discuss the cases where it is not observed in the domain. The impact of these particle dynamics on coastal carbon storage and export is discussed.

Land use determines the composition and stability of organic carbon in earthworm casts under tropical conditions

Environmental conditions play an important role in controlling the fate of organic carbon (OC) in earthworm casts. However, the mechanisms that lead to the destabilization of OC in casts deposited in different land use systems are poorly understood. In this study, we investigated the impact of land use on the fate and composition of particulate organic matter (POM) and mineral-associated organic matter (MAOM) in earthworm casts under tropical conditions. We conducted a 400-day field exposure experiment in a woodland and a meadow in northern Vietnam with earthworm casts and soil aggregates without trace of earthworm activity (reference soil) under natural rainfall conditions. We analyzed the element content, stable carbon isotope composition and mid-infrared signatures of the two types of materials after 9 and 400 days of field exposure. The results showed that the casts were initially enriched in OC as compared to the reference soils, with the MAOM fraction accounting for over 90% of the earthworm-induced OC accumulation. The POM fraction occluded in casts consisted of fresh plant material and disappeared during the 400 days of field exposure. Enrichment and composition of OC differed between woodland and meadow casts. POM and MAOM showed contrasting persistence in the two land use systems. While woodland casts showed the highest potential to stabilize OC, the actual amount of cast OC stabilized for more than 400 days was more important under meadow. The processes affecting cast OC dynamics were contrasting in both systems. Under woodland, cast OC destabilization was most probably related to microbial degradation, while under meadow, OC accumulation was most probably related to root activity. Our study highlights that the impact of earthworms on the origin, composition, and fate of cast OC in tropical environments is strongly influenced by land use.

Grazing intensity and nitrogen fertilization timing to increase soil organic carbon stock and nitrogen in integrated crop-livestock systems

ABSTRACT Integrated crop-livestock systems (ICLS) foster synergistic relationships to increase nitrogen (N) cycling and soil organic carbon (SOC) accrual in agricultural setups. This study evaluated how the grazing intensity and N fertilization (rates and timing) affect both SOC and N fractions, and soil organic matter chemical composition in an ICLS managed under no-tillage in an Oxisol, six years after initiation. The ICLS was compared to a nearby pasture (PA) and a native forest (NF). The treatments consisted of two grazing intensities: Low Sward Height (LH) and High Sward Height (HH) were maintained with high and low stocking rates, respectively. The HH varied between 0.20 and 0.60 m, and LH between 0.10 and 0.30 m according to the plant forage species throughout the experiment. Fertilization using 200 kg ha -1 N-urea, not splitting up, was conducted at two timings, either at the winter pasture establishment (autumn), about 35 days after sowing or during the summer cash crop cycle (spring). Total N amount per year, including both phases, pasture and cash crop was the same for all treatments. The SOC and N contents were assessed in soil and particulate organic matter (POM), while carbon (C) and N stocks were specifically determined in the soil. Soil organic matter composition was characterized by FTIR. The combination of HH and N fertilization during the pasture phase increased the content of C from 36.1 to 39.9 ± 0.7 g C kg -1 and of N from 2.7 to 3.2 ± 0.1 g N kg -1 . The SOC stocks varied from 37.3 to 41.1 ± 0.7 Mg C ha -1 , and the N stocks from 2.1 to 3.3 ± 0.1 Mg N ha -1 at 0.0-0.10 m soil layer. The SOC content of the POM and the soil organic matter chemical composition determined by FTIR were mainly affected by the grazing intensity. The HH led to an increased in C content within the POM fraction, reaching values of 51.6 ± 1 and 49.2 g C kg -1 , respectively to N crop fertilization and N pasture fertilization. Land-use changed how organic functional groups were stored in soil organic matter fractions. The NF had a greater abundance of aliphatic and phenol in the MAOM, while pasture and ICLS systems had greater aliphatic in the POM fraction. In ICLS, SOC accrual was positively associated with more recalcitrant organic functional groups of phenol, aromatic, and carbonyl C-O. The HH increases SOC accrual, while N-fertilization on pasture ensures adequate nutrition of plants and animals during the winter ICLS phase, at the same time as providing greater residual N for subsequent cash crops through enhanced catalyzed by ruminants. Therefore, grazing and fertilization management strategies should be considered to promote sustainable agriculture intensification with ICLS.

Macrofauna amplify plant litter decomposition and stabilization in arctic soils in a warming climate

The soil organic carbon (SOC) pool of the Arctic region is currently protected by low temperatures, but is likely to decrease due to greater organic matter (OM) decomposition under a warmer climate. Negative feedback for climate warming can, however, be reversed by SOC accrual as climate warming leads to shifts in arctic vegetation (from grass to shrub) and soil faunal (introduction of macrofauna) communities affecting plant-soil C allocation. To decipher these contrasting effects, we performed a laboratory experiment with soils from dry tundra to test the interacting effects of plant litter quality (high-quality grass litter vs. the intermediate- and low-quality litter of shrubs) and soil fauna functional grouping (micro-, meso- and macrofauna [millipede]) on the processes of litter decomposition and OM stabilization.Our findings showed that macrofauna largely promoted decomposition of shrub litter, while soil micro- and mesofauna were mainly responsible for the decomposition of grass litter. Our study thus confirmed that, when introduced and established in a warmer Arctic, macrofauna may become an important agent in shrub litter decomposition. Our data also showed that with shrub litter, higher C content was stabilized as particulate OM (POM) in aggregates, whereas in grass litter and low-quality shrub litter, higher C content was stabilized as mineral-associated OM (MAOM). Both these effects were larger in the presence of macrofauna and with a higher abundance of fungi. This suggests that consequent shrub OM stabilization in occluded POM and MAOM fractions will be carried out jointly by macrofauna and fungi, which will probably lead to more efficient OM stabilization in Arctic soils than in the case of grass litter OM stabilization by micro- and mesofauna and bacteria. In conclusion, our study suggests that vegetation changes and the introduction of macrofauna in a warming climate will most probably lead to higher OM stabilization in Arctic soils.

Distribution of Soil Organic Carbon Density Fractions in Aggregates as Influenced by Salts and Microbial Community

Understanding soil organic carbon (SOC) at the aggregate level is crucial for soil health in secondary-salinized greenhouse development. Nevertheless, the specific patterns and contributions of ion content and microbial communities on SOC density fractions at the aggregate level remain unclear in secondary-salinized soil. We investigated variations in salts [electrical conductivity (EC) and ions] and microbial communities across various aggregate classes in both a 16-year-use greenhouse and open-field soils. We also examined SOC density fractions, including the light fraction (LF), the heavy fraction of particulate organic matter (POM), and mineral-associated organic matter (MOM) across different aggregates. The findings revealed that a lower Ca2+/K+ along with elevated EC levels (average 2.49 mS cm−1) reduced the macroaggregate percentage in greenhouse compared to open-field conditions, with a lower EC of 0.58 mS cm−1. Bacterial diversity and community composition exhibited no variation across different aggregate sizes at both sites. Conversely, fungal diversity and relative abundance (primarily dominated by Ascomycota of 78.50%) substantially increased in microaggregates (&lt;0.25 mm) compared to macroaggregates (&gt;0.25 mm). Macroaggregates exhibited a higher proportion of LF and MOM (3.3–18.2%, 24.9–34.5%, and 2.9–4.0% for LF, MOM, and POM, respectively) than microaggregates. Correlation and redundancy analyses revealed that fungal diversity, particularly the relative abundance of Ascomycota in aggregates &lt; 0.25 mm, significantly and positively influenced (p &lt; 0.05) the proportion of MOM carbon in terms of the overall SOC (J-type). This study provides valuable insights into the distribution patterns of SOC within the secondary salt-affected soils.

Distribution of soil organic carbon between particulate and mineral-associated fractions as affected by biochar and its co-application with other amendments

The application of biochar to soils, either alone or combined with other amendments, represents a management practice aimed at storing carbon (C) while enhancing soil fertility. However, the long-term effects of biochar application on soil organic C protection against microbial decomposition are uncertain. This study investigated, in a 9-year-long-term field experiment, the protection of organic C by minerals and iron (Fe) in soils amended with biochar alone or combined with either municipal solid waste compost or sewage sludge. Particulate (not protected by minerals) and mineral-associated organic matter fractions were separated, quantified, and finally the Fe-mediated protection mechanisms were examined by Fe K-edge extended X-ray absorption fine structure spectroscopy. With respect to the unamended control soil, soils amended with biochar, especially when combined with municipal solid waste compost and sewage sludge, had 3 times greater content of particulate organic C. Biochar combined with municipal solid waste compost and sewage sludge also increased mineral-associated organic C content (1.5×), although the magnitude of the effect was smaller than for the particulate organic C fraction. The contents of Fe(III)-organic matter complexes in particulate and mineral-associated organic matter fractions of the amended soils were similar to those of the unamended soils. Hematite represented the main Fe oxide phase in the particulate organic matter fraction of all the soils as well as in the mineral-associated organic matter fraction of the unamended soils, whereas ferrihydrite was more abundant in the mineral-associated organic matter fraction of the amended soils. As a whole, the obtained results in general, and the positive effect on the mineral-associated organic matter possibly mediated by ferrihydrite occurrence in particular, highlight the potential of biochar, alone or in combination with other amendments, as a strategy to store and preserve C in soils.

Evidence of Potential Organo-Mineral Interactions during the First Stage of Mars Terraforming

Future space missions to Mars will depend on the development of bioregenerative life support systems. Mars regolith contains most of the nutrients needed for plant growth, but not organic matter (OM). Although Mars simulants have been deeply characterized and tested as growing media, no data are available about their possible modification occurring during terraforming, including the interaction of exogeneous OM with iron (Fe) oxides, particularly abundant in Mars regolith. The aim of this study was to investigate the mineral transformation and the OM evolution occurring in the early stages of the terraforming process. Potato was grown for 99 days on Mojave Mars Simulant MMS-1, alone (R100) and mixed with a compost 70:30 v:v (R70C30), and on a fluvial sand, alone (S100) and mixed with compost (S70C30), for comparison. Bulk (BK) and potato tubero/rhizo-sphere (RH) soils were fractionated to obtain particulate OM (POM) and mineral-associated OM (MAOM). Bulk samples and corresponding fractions were characterized for total nitrogen and organic carbon (C) and analyzed by Fe K-edge X-ray absorption near-edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) spectroscopy. Organic C increased by 10 and 25 times in S70C30 and R70C30, respectively, compared to S100 and R100. Most of the organic C accumulated in the POM fraction of both growing substrates, while its content in the MAOM was 3 times higher in R70C30 than in S70C30. No significant differences between BK and RH were found. Finally, ferrihydrite mediated exogenous OM stabilization in regolith-based substrates, while Fe(III)-OM complexes were detected exclusively in sand-based growing media. Understanding mechanisms and testing potential sustainable practices for creating Mars regolith similar to terrestrial soil will be fundamental to sustain food crop production on Mars.

Deciphering the role of particulate organic matter in soil nitrogen transformation in rice–rapeseed and rice–wheat rotation systems

Crop rotation affects the decomposition of soil organic matter (SOM) and thereby alter the composition of SOM fractions. It remains unclear how different SOM fractions impact soil nitrogen (N) transformation in various rotation systems. The aim of this study was to ascertain the role of particulate organic matter (POM)-a labile SOM fraction-in soil N transformation under various crop rotations. A paired plot experiment was conducted under two common cropping patterns, i.e., rice–rapeseed rotation (RR) vs. rice–wheat rotation (RW). Soil chemical composition and organic matter fraction before rice transplanting were compared between RR and RW systems after four years of crop rotations (2017–2021). With the same N inputs, the rice yield and N uptake under RR were 16.4 % and 13.2 % higher than those under RW, respectively. Compared with RW, RR resulted in higher carbon (C) and N contents in soil POM, despite minimal differences in total SOM. A larger potentially mineralizable N pool and a higher N mineralization rate occurred under RR than under RW, based on the results of soil net mineralization experiment. When POM was incubated alone, its contribution to potentially mineralizable N was 65.1 % and 61.3 % in RR and RW soils, respectively. Infrared spectroscopy revealed that in contrast with RW, RR promoted the accumulation of organic matter with high bioavailability (e.g., amides, carbohydrates, polysaccharides) in soil POM. This might be responsible for the higher gross mineralization and nitrification rates but lower gross immobilization rate under RR than under RW. Consequently, RR not only increased the contents of POMC and POMN but also improved the quality of POM fraction in soils. Findings of the present study demonstrate that POM plays a distinct role in soil N mineralization in various rotation systems. The discrepancy in POM content and composition resulting from various crop rotations leads to differences in soil N mineralization, which in turn affects the N supply and rice yield.

Insight into the effect of particulate organic matter on sludge granulation at the low organic load: Sludge characteristics, extracellular polymeric substances and microbial communities response

This paper investigated the effect of particulate organic matter (POM) on sludge granulation under low organic load. The results showed that POM promoted the formation of aerobic granular sludge (AGS) with a chemical oxygen demand (COD) fraction of 25%, and POM also enhanced the sludge settleability and biomass retention. However, when the COD fraction of POM increased to 50% and 75%, the AGS performance deteriorated. The analysis of extracellular polymeric substances revealed that the POM (accounted for ≤ 50% of COD in the influent) suppressed the secretion of extracellular protein. Analysis of the microbial community showed that species diversity was lower in the POM-fed system, with Rhodocyclaceae being the predominant bacteria responsible for carbon source degradation. Additionally, molecular ecological network analysis demonstrated that when the COD fraction of the POM exceeded 50%, the connectivity and modularity between microbial species decreased, which may explain the sludge performance deterioration.

Priming effects decrease with the quantity of cover crop residues – Potential implications for soil carbon sequestration

Meta-analyses suggest a global potential of cover crops to increase soil organic carbon (SOC) stocks, yet with a large variation across studies, which underlines the need to understand the effect of cover crops on carbon (C) sequestration under specific soil and climate conditions. We studied the C sequestration potential from cover crops, based on a Danish long-term field experiment (LTE) initiated in 1997, where SOC and C in the fractions of particulate organic matter (POM) and mineral associated organic matter (MAOM) was measured to 1-m depth. Next, we performed a mesocosm study where the fate of 14C-labeled cover crop residues (fodder radish, Raphanus sativus L.) and SOC priming were traced in two texturally similar soils from the LTE with different SOC concentrations (2.0 vs. 2.6% SOC). The results showed that cover cropping for up two decades had negligible effect on SOC in POM and MAOM fractions. Yet, the mesocosm study showed considerable overall SOC increases (20–25% of added C) when the cover crop C input exceeded rates of 0.2–0.3 mg C g−1 in the two soils. This was due to a combination of new SOC formation and priming effects shifting from positive to negative. The input rates of 0.2–0.3 mg C g−1 correspond to the C input from cover crops with an aboveground yield of approximately 0.7–1.1 Mg dry matter ha−1, which is a level not always achieved at the field site. The combined observations from the field and mesocosm study suggest that SOC buildup was not constrained by soil C saturation, but rather by low cover crop productivity and/or positive priming effects. Therefore, agricultural management practices (e.g., species choice and sowing time) should be adopted to achieve a sufficient cover crop C input to secure that the positive priming effect is not exceeding the rate of SOC formation.

Nitrogen addition increases the glucose-induced priming effect of the particulate but not the mineral-associated organic carbon fraction

Nitrogen availability affects the mineralization of soil organic matter differently depending on soil type and soil layer. It is unknown whether such differences result from different responses of individual carbon fractions to N supply. This study examined the priming effect (PE) on the decomposition of particulate organic matter (POM) and mineral-associated organic matter (MAOM) fractions in response to N addition. Two soils, one from a woodland and the other from a cropland, were fractionated to obtain two POM fractions differing in C:N ratio and two MAOM fractions differing in mineral C protection. Two fractions of each soil were incubated for 14 days after the initial supply of 13C-glucose and Ca(NO3)2 at 0, 20 and 60 mg N kg−1. Glucose addition induced a stronger PE per unit soil organic C in the MAOM than POM, particularly in the woodland soil (6.2 vs. 0.8 mg C g−1 C). The glucose-stimulated increases in the abundances of bacteria, fungi, and four functional genes (LMCOs, ureC, npr and chiA) were also larger in the MAOM than POM. The PE was similar in the MAOM (C:N 11.6–12.4) from the two soils and was not affected by N addition. By contrast, N addition increased the PE in the POM (C:N 21.6) of the woodland soil (e.g., by 7-fold at 60 mg N kg−1), along with marked increases in the abundances of fungi, the four functional genes, and the ratio of fungi to bacteria. Compared with the woodland soil, the addition of 60 mg N kg−1 to the POM of the cropland soil (C:N 16.9) stimulated the PE to a lesser extent (by 30%). These results suggest that N input may have a greater potential in stimulating the PE in soils with a higher proportion of POM with a greater C:N ratio.

Molecular composition and possible transformations of labile soil organic matter fractions in Mediterranean arable soils: Relevance and implications

With the increased global interest in sequestering carbon in soil, it is necessary to understand the composition of different pools of soil organic matter (SOM) that cycle over suitably short timeframes. To explore in detail the chemical composition of agroecologically relevant yet distinct fractions of SOM, the light fraction of SOM (LFOM), the 53-μm particulate organic matter (POM), and the mobile humic acid (MHA) fractions were sequentially extracted from agricultural soils and characterized using both 13C cross polarization magic angle spinning nuclear magnetic resonance (CPMAS NMR) spectroscopy and also Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). The NMR results showed a decrease in the O-alkyl C region assigned to carbohydrates (51–110 ppm) and an increase in the aromatic region (111–161 ppm) proceeding from the LFOM to the POM and then to the MHA fraction. Similarly, based on the thousands of molecular formulae assigned to the peaks detected by FT-ICR-MS, condensed hydrocarbons were dominant only in the MHA, while aliphatic formulae were abundant in the POM and LFOM fractions. The molecular formulae of the LFOM and POM were mainly grouped in the high H/C lipid-like and aliphatic space, whereas a portion of the MHA compounds showed an extremely high (17–33, average of 25) double bond equivalent (DBE) values, corresponding to low H/C values of 0.3–0.6, representative of condensed hydrocarbons. The labile components appeared most pronounced in the POM (93% of formulae have H/C ≥ 1.5) similar to the LFOM (89% of formulae have H/C ≥ 1.5) but in contrast to the MHA (74% of formulae have H/C ≥ 1.5). The presence of both labile and recalcitrant components in the MHA fraction suggests that the stability and persistence of soil organic matter is influenced by a complex interaction of physical, chemical, and biological factors in soil. Understanding the composition and distribution of different SOM fractions can provide valuable insights into the processes that govern carbon cycling in soils, which can help inform strategies for sustainable land management and climate change mitigation.