An experimental study was conducted to explore sediment particle motion in an open channel and its relationship to bedform characteristics. High-definition submersed video cameras were utilized to record images of particle motion over a dune’s length scale. Image processing was conducted to account for illumination heterogeneity due to bedform geometric irregularity and light reflection at the water’s surface. Identification of moving particles using a customized algorithm was subsequently conducted and then the instantaneous velocity distribution of sediment particles was evaluated using particle image velocimetry. Obtained experimental results indicate that the motion of sediment particles atop dunes differs depending on dune geometry (i.e., two-dimensional or three-dimensional, respectively). Sediment motion and its relationship to dune shape and dynamics are also discussed.