Particle Image Velocimetry Research Articles

Direct Measurements of Dust Settling Velocity Under Low‐Density Atmospheres Using Time‐Resolved Particle Image Velocimetry

AbstractDust dynamics influence planetary atmospheres. However, the settling velocity of dust—and thus its residence time in the atmosphere—is often mispredicted. Challenging, indirect experiments involving few ideal particles revealed that dust settling velocity deviates from Stokes' law under rarefied atmospheres. While useful, such experiments are inadequate to simulate more complex scenarios, including variable particles sizes and shapes. Here, we present direct measurements of settling velocity for spherical particles under Earth‐to‐Mars atmospheric pressures using time‐resolved particle image velocimetry (TR‐PIV), and validate their robustness with existing models. Our results demonstrate that TR‐PIV provides a relatively simple approach to quantifying dust settling velocity from direct observations of over 10,000 particles, enabling systematic investigations of dust settling under realistic scenarios. Such experiments will have significant implications for our understanding of Mars' past, present, and future ‐ from providing a tool to decipher its sedimentary record to enhancing predictive capabilities of atmospheric models.

Simultaneous flow and particle measurements for multiphase flows in hydraulic engineering: A review and synthesis of current state

While multiphase flows are abundant in both natural environments and engineering applications, their analysis and quantification present challenges. In particular, simultaneously measuring the flow field and quantifying particle behavior pose many challenges. Methods based on Particle Image Velocimetry (PIV) and Particle Tracking Velocimetry (PTV) principles have been used in the last two decades to measure flow fields and dispersed (particle) phases, respectively. Despite the extensive application of these principles to multiphase flow, there are numerous approaches and techniques used to synchronize and combine PIV and PTV measurements. Combined PIV and PTV data acquisition also requires consideration of phase discrimination to obtain distinct data for the fluid and dispersed phases. In the literature, various methods have been proposed and applied to achieve phase discrimination. This review paper aims to consolidate and classify various phase discrimination techniques used in specialized applications in hydraulic engineering. These methods are categorized into optical (spectral, temporal, and hybrid) and post-processing methods, with a particular emphasis on their applicability within the realm of hydraulic engineering. Moreover, this review expands on several emerging PIV/PTV technologies and applications, where the combination of equipment and algorithms has led to significant strides in the non-intrusive measurement of multiphase flow. By consolidating and critically evaluating current methods for particle discrimination, this paper aims to enhance the scientific community's understanding of simultaneous phase velocity measurements, thereby setting the stage for advancements in multiphase flow visualization techniques.

Custom-made spherical fluorescent tracer particles for laser Velocimetry Laboratory Experiments

This article introduces a method for creating custom-made spherical fluorescent particles tailored for fluid mechanics optical velocimetry laboratory measurements techniques such as Particle Image Velocimetry (PIV) and Particle Tracking Velocimetry (PTV) measurement techniques. The incorporation of the fluorescent dye Rhodamine B into the particle bulk significantly reduces direct light reflections and enhances particle detection when enlightened by a laser and using a band-pass filter on the optical measurement device. Furthermore, the study offers a clear guide for adjusting particle diameter by modifying various parameters such as the stirrer used, its stirring speed, the surfactant concentration, and the type of polymer. This allows for the customization of particles best suited for water multiphase flow characterization and transport studies. The resulting particles exhibit excellent sphericity, making them suitable for a wide range of fluid mechanics investigations, from small to large scale. Experimental validations, including size measurements, density assessments, emission spectra analyses, and applicability in a confined flow affirm the effectiveness of the proposed methodology by exhibiting a signal-to-noise ratio four to five times higher than fluorescent surface-stained and non-fluorescent particles.

Assessing the performance of infrared thermography and PIV methods to identify and characterize the air inlets and outlets in wall assemblies

Building airtightness is crucial for reducing energy demand in the building sector and preventing moisture damage and indoor environment quality issues. Mandatory airtightness tests are now commonly performed in many countries to quantify the total envelope leakage rate of real buildings. For a more precise diagnosis, air leakages are usually identified by infrared thermography or visualized by smoke. However, a finer analysis is also needed to characterize precisely the air flow through specific leaks. Numerical models have been developed for this purpose but experimental data are necessary to validate them. In this paper, two experimental approaches are tested on simple light-wall assembly configurations to address this need: infrared thermography (IRT) and the innovative use of particle image velocimetry (PIV) with a specific experimental set-up and protocol developed. The results are compared with each other as well as with numerical simulations. Results showed that both techniques can be applied for the experimental characterization of the air inlet/outlet of a wall assembly with different advantages and drawbacks. IRT is easy to implement, non-intrusive, possible for in-situ investigations, but the correlation between the thermographs and the air dispersion at the outlet is not straight forward due to thermal inertia and heat conduction issues. PIV gives direct results on the velocity field and enables quantitative analysis of air flow rates for various configurations, with some limitations: a difficult implementation; restrictions on the possible infiltration velocity range and difficulties to visualize the flow exactly at the interface with the wall assembly.

Experimental investigation on aerodynamic noise of a small-scale multi-blade centrifugal fan

In this study, the aerodynamic noise of a small-scale centrifugal fan was experimentally investigated using acoustic testing techniques and time-resolved stereoscopic particle image velocimetry (SPIV). During the acoustic experiments, both far-field noise and near-field pressure fluctuations of the test fan were measured. The overall far-field noise towards the fan inlet side was found to be higher than that of the back side. The pressure fluctuations on the fan upper casing exceeded those on the side wall due to the uncontracted volute tongue, indicating pronounced flow-to-wall interactions. Moreover, based on a simultaneous measurement, the coherence between the near-field pressure fluctuations and far-field noise highlighted the significant contributions of impeller rotation to noise radiation. SPIV measurements uncovered the time-averaged and transient flow fields at the fan's inlet and outlet. The time-averaged results demonstrated the concentrated inlet flow and outlet flow separation, leading to high flow unsteadiness. Transient flow fields displayed an asymmetric jet-wake region characterized by both quasi-steady flow and rotational flow behaviours. The instantaneous flow results were analyzed using the dynamic mode decomposition (DMD) method, which clearly recognized the jet-wake patterns with frequencies corresponding to the rotational frequency. The observed consistency in frequency characteristics among noise, pressure fluctuations, and unsteady flow affirms that flow dynamics are crucial to the primary noise mechanisms.

Experimental investigation of hydrodynamics and suspension characteristics in a boiling stirred tank reactor with helical coils

The boiling stirred tank reactor (BSTR) is widely used in the chemical industry, while the relevant basic data of hydrodynamics and suspension characteristics for reactor design are quite limited. In this work, the hydrodynamics and suspension characteristics in a BSTR with helical coils (BSTR@HC) were studied using three types of impellers. Particle image velocimetry and visualization experiments reveal different flow fields and nucleation processes of three impellers. Suspension experiments show that superficial vapor velocity, solid loading, and particle size play an important role for just-suspension impeller speed Njsv and power consumption Pjsv, while cloud height is mainly determined by superficial vapor velocity. Among the three impellers, radial impeller shows the best suspension performance in BSTR@HC. Correlations of Njsv and Pjsv were developed with relative mean deviations of 2.7% and 4.6%, respectively. Furthermore, a model for the estimation of energy efficiency was developed by evaluate specific power consumption εjsv.

Electrical properties determine the liquid flow direction in plasma–liquid interactions

During atmospheric pressure plasma impingement, plasma induced liquid flow will influence the transport and distribution of plasma generated charged and reactive species in liquids. We use particle image velocimetry and supplementary pH, conductivity and temperature measurements to investigate electrical properties of an AC kHz plasma jet interacting with water and electrolytes. We observe that the dominant driving mechanism in low conductive solutions are surface forces such as shear stresses and stagnation-pressure induced dimpling. These give upwards flows beneath the plasma–liquid interaction point. In highly conductive solutions, such as water with dissolved salts, the dominant driving mechanism is electro-hydrodynamic forces, with flows directed downwards underneath the plasma jet in our system. We therefore demonstrate that the direction of initial plasma induced liquid flows can be controlled through the addition of salt ions. In electrically grounded salt solutions, we also observe time resolved flow direction switching, possibly due to modification of salt solutions via electrolytic and plasma induced reactions changing the dominant flow mechanism over time.

Flow control over tandem cylinders using plasma actuators

The flow over two circular cylinders, arranged in a tandem setup, is controlled with the help of dielectric-barrier-discharge (DBD) plasma actuators mounted on the upstream cylinder at a Reynolds number (Re) of 4700. The plasma actuators are mounted at ±80∘ from the forward stagnation point of the upstream cylinder. Three tandem configurations are tested, where the distance, L, by which the cylinder centers are separated are fixed at 3, 4, and 5 cylinder diameters (D). For each configuration, the plasma actuators are operated at two distinct blowing ratios (BR) of 0.8 and 1.4, which are named as the low-power and high-power forcing cases, respectively. Results include static-pressure measurements on the downstream cylinder and wake surveys using Particle Image Velocimetry (PIV). High-power forcing changes the flow pattern in the L=3D upstream wake from reattached to co-shedding flow, enabling alternating vortex shedding to occur between the tandem cylinders. High-power forcing also significantly weakens vortex shedding from the upstream cylinder for L=4D and L=5D. This weakening is manifested through 39.27% and 35.32% reductions in the total area of vorticity contours for L=4D and L=5D, respectively. However, the effect of this cancellation is most prominent on the downstream cylinder when the separation distance is L/D=3. During forcing with BR = 1.4, the static pressure on the downstream cylinder resembles that of a flow over a regular cylinder for all the cases tested. Hence, at this blowing ratio, the wake signature of the upstream cylinder is severely diminished, by delaying the shear-layer separation point. During forcing with BR = 0.8, no significant effect on the downstream cylinder is observed.

Flowfield and Noise Dynamics of Supersonic Rectangular Impinging Jets: Major versus Minor Axis Orientations

The current study explores the flowfield and noise characteristics of an ideally expanded supersonic (Mach 1.44) rectangular jet impinging on a flat surface. The existing literature is primarily concentrated on axisymmetric jets, known for their resonance dominance, pronounced unsteadiness, and acoustic signatures. In contrast, non-axisymmetric jets remain relatively less understood, particularly those impinging on a ground surface. By employing Schlieren imaging, high-frequency pressure measurements using high-bandwidth transducers, and particle image velocimetry (PIV), this research comprehensively examines the flow-acoustic phenomena. Schlieren imaging revealed distinct, coherent structures and strong acoustic waves, while pressure measurements at the impingement surface exhibited high-amplitude fluctuations, peaking at approximately 186 dB. Acoustic analysis identified multiple high-amplitude tones with unique directional characteristics, suggesting the potential for multiple simultaneous modes in rectangular jets. Furthermore, the PIV data elucidated differences in the jet shear layer and wall jet development attributed to the nozzle orientation. These findings contribute to a deeper understanding of non-axisymmetric jet behavior, offering insights relevant to fundamental flow physics and practical applications such as vertical takeoff and landing aircraft.

Design and characterization of a low-cost particle image velocimetry system

Particle Image Velocimetry (PIV) is considered the gold standard technique for flow visualization. However, its cost (at least tens of thousands of dollars) can prove inhibitive in its standard form. This article presents an alternative design, leveraging off-the-shelf and open-source options for each key component involved: camera, laser module, optical components, tracer particles, and analysis software. Flow visualization is a crucial technique to connect theory to practice in teaching and researching fluid mechanics. Despite the ubiquity of this field within engineering curricula, many undergraduate institutions globally forego utilizing such equipment, given the barriers to setting it up. The availability of this low-cost alternative (∼$500) that can be built in-house offers a path forward. Characterization was done by visualizing the rotational flow generated by a magnetic stirrer in a cylindrical beaker. The velocity magnitude around the stirrer bar measured by the low-cost PIV system was compared to expected values calculated analytically. The percent difference was between 1–2% when the flow stayed two-dimensional but increased as the flow began developing into more of a 3-D flow. Repeatability varied no more than 6% between experiments. This platform holds the potential for reliable replication across institutions broadly.

Microscopic Particle Image Velocimetry Analysis of Multiphase Flow in a Porous Media Micromodel.

Pore-scale oil displacement behavior was investigated in a porous media micromodel using microscopic particle image velocimetry (μPIV). Porous media micromodels consisting of an ordered square array of cylindrical pillars with 50 and 70% porosities were fabricated with photolithography. The oil displacement was performed with the injection of water at flow rates of 37.5, 75, and 150 μL/h. These flow rates correspond to Reynolds number of 1.1 × 10-2, 2.2 × 10-2, and 4.4 × 10-2, respectively in the 50% porous channel, and 1.84 × 10-3, 3.69 × 10-3, and 7.38 × 10-3, respectively in the 70% porous channel. The capillary numbers for these flow rates are 2.18 × 10-5, 4.36 × 10-5, and 8.72 × 10-5, respectively in the 50% porous channel, and 1.56 × 10-5, 3.12 × 10-5, and 6.23 × 10-5, respectively in the 70% porous channel. The micromodel is initially saturated with oil, with the invading water phase following the path of least resistance as it displaces the oil. The μPIV data were used to construct probability density functions (PDFs) which show an initial, nonzero, peak in transverse velocity as the water enters the micromodel. The PDFs broaden with time, indicating that the water is spreading, before retracting to a peak velocity of 0 mm/s, indicating that the water displacement has achieved equilibrium. We developed a model based on conservation of mass to describe the efficiency of the displacement process. All flow conditions demonstrate peak displacement efficiency when the amount of oil phase displacement is ∼9 pore volumes in 50% porous channel and ∼4 pore volumes in 70% porous channel.

Applications of RIM-Based Flow Visualization in Fluid-Solid Interaction Problems: A Review of Formulations and Prospects

Over the past three decades, optical visualization measurements based on the Refractive Index Match (RIM) method have played a significant role in the experimental studies of fluid-solid interaction. The RIM method, which coordinates the refractive indices of the liquid and solid materials in the experiment, dramatically reduces the observation error due to optical refraction. However, the existing literature on RIM has not systematically reviewed the various applications of this technique. This review aims to fill this gap by providing a comprehensive overview of the RIM technique, examining its role in material selection for fluid-solid interaction studies, and scrutinizing its applications across various engineering disciplines. The paper begins with a brief introduction to the RIM technique and then turns to material selection and its various applications in fluid-solid interaction. It also enumerates and analyzes specific RIM-based optical measurement techniques such as Laser Doppler Velocimetry (LDA), Particle Tracking Velocimetry (PTV), and Particle Image Velocimetry (PIV) from various research perspectives in previous studies. In addition, it summarizes RIM formulations categorized by different applications in liquid-solid interaction fields. RIM-based measurement techniques generally offer intuitive, non-intrusive, cost-effective, and convenient advantages over traditional methods. The paper also critically evaluates the strengths and limitations of different materials used in RIM experiments and suggests directions for future research, emphasizing the need to develop environmentally friendly and cost-effective RIM materials.

Soot Measurements and a Simplified Analytical Formulation-Based Modeling in Laminar Counterflow Diffusion Flames

ABSTRACT The paper presents soot volume fraction measurements and a simplified soot formation model. Soot formation is a complex physicochemical process predominantly modeled using detailed chemistry and stochastic methods. The current work presents a simplified analytical function-based approach to calculate the net soot formation rate by coupling the local mixture-fraction and temperature fields. The validation experiments were carried out in a unique large-diameter (40 mm) counterflow burner for ethylene-air flames at various strain rates and normalized separation (L/D) distances. The soot volume fractions (fv) were determined using Laser Induced Incandescence (LII) technique calibrated by the Light Extinction (LE) method. Further model validations were carried out against the experimental measurements in the literature. The modeling work was performed on OpenFOAM®. The model predicted soot distributions for various peak flame temperatures, stoichiometric mixture-fractions, burner dimensions, fuel-oxidizer compositions, and strain rates with good accuracy. The study underscores the importance of considering two soot formation rate peaks, one at high temperature and one at low temperature, for accurate predictions, unlike a single peak employed in common analytical models. Furthermore, the effect of velocity boundary conditions on soot zone locations and the significance of providing realistic velocity conditions for the simulations are also discussed. Particle Image Velocimetry (PIV) measurements were performed to determine the actual nozzle-exit velocity profiles.

Experimental study on dynamics of a free-falling finite-size sphere associated with its collision with a wall in a boundary layer

To reveal the physical mechanisms of particle transport in the pneumatic and hydraulic transports, this study experimentally investigated the dynamics of a free-falling finite-size sphere associated with its collision with a wall and the surrounding flow field in a boundary layer flow. The simultaneous motion of finite-size sphere and its inducing flow field near the wall were measured by using a time-resolved particle image velocimetry. The results revealed that the free-falling velocity of sphere increases with Reynolds number before the first impaction, and then is almost unrelated to Reynolds number. The effect of gravity on sphere motion becomes weak with increasing Reynolds number. As the sphere approaches to the wall, the vortex appeared at the front of sphere moves to left side near the wall and reduces the sphere falling speed. After the sphere collided on the wall, the upward motion of sphere induces another vortex pair, which generates strong rotation of sphere.

Weak shock compaction on granular salt

This study conducted integrated experiments and computational modeling to investigate the speeds of a developing shock within granular salt and analyzed the effect of various impact velocities up to 245 m/s. Experiments were conducted on table salt utilizing a novel setup with a considerable bore length for the sample, enabling visualization of a moving shock wave. Experimental analysis using particle image velocimetry enabled the characterization of shock velocity and particle velocity histories. Mesoscale simulations further enabled advanced analysis of the shock wave’s substructure. In simulations, the shock front’s precursor was shown to have a heterogeneous nature, which is usually modeled as uniform in continuum analyses. The presence of force chains results in a spread out of the shock precursor over a greater ramp distance. With increasing impact velocity, the shock front thickness reduces, and the precursor of the shock front becomes less heterogeneous. Furthermore, mesoscale modeling suggests the formation of force chains behind the shock front, even under the conditions of weak shock. This study presents novel mesoscale simulation results on salt corroborated with data from experiments, thereby characterizing the compaction front speeds in the weak shock regime.

Model Test Study on the Bearing Mechanism of Inclined Variable Cross-Section Piles Using Transparent Soil

In view of the influence of the inclination and variable section on the pile stability and bearing capacity, this paper introduces particle image velocimetry (PIV) technology, and designs a transparent soil visualization model test. The experimental results show that, when the pile has a variable cross-section and inclination angle, the friction resistance on both sides of the pile increases. The vertical-load-carrying capacity of the 2% and 4% inclined piles with a variable cross-section is greater than that of the piles with inclinations greater than 8%. For model piles with the degrees of inclination of 2% and 4%, the variable-section inclined piles with diameters of 17 mm and 15 mm show significantly less settlement than the equal-section inclined piles. For the model pile with an inclination of 8%, the settlement of the inclined piles with a variable cross-section diameter of 17 mm is slightly smaller than that of the equal cross-section inclined piles. The change in variable cross-section and inclination angle has a large effect on the soil displacement around the pile, and the conclusions of this paper can provide guidance for the engineering application of variable cross-section piles.

Reconstruction of particle distribution for tomographic particle image velocimetry based on unsupervised learning method

The development of deep learning has inspired some new methods to solve the 3D reconstruction problem for Tomographic Particle Image Velocimetry (Tomo-PIV). However, the supervised learning method requires a large number of data with ground truth as training information, which is very difficult to gather from experiments. Although synthetic datasets can be used as alternatives, they are still not exactly the same with the real-world experimental data. In this paper, an Unsupervised Reconstruction Technique based on U-net (UnRTU) is proposed to reconstruct volume particle distribution explicitly. Instead of using ground truth data, a projection function is used as an unsupervised loss function for network training to reconstruct particle distribution. The UnRTU was compared with some traditional algebraic reconstruction algorithms and supervised learning method using synthetic data under different particle density and noise level. The results indicate that UnRTU outperforms these traditional approaches in both reconstruction quality and noise robustness, and is comparable to the supervised learning methods AI-PR. For experimental tests, particles dispersed in cured epoxy resin are moved by an electric rail with a certain speed to obtain the ground truth data of particle velocity. Compared with other algorithms, the reconstructed particle distribution by UnRTU has the best reconstruction fidelity. And the accuracy of the 3D velocity field estimated by UnRTU is 12.9% higher than that from the traditional MLOS-MART algorithm. It demonstrates significant potential and advantages for UnRTU in 3D reconstruction of particle distribution. Finally, UnRTU was successfully applied to the high-speed planar cascade airflow field, demonstrating its applicability for measuring complex fluid flow fields at higher particle density.

Influence of laser power and beam path under nonuniform AC electric fields on 3D microvortex flow

This paper describes the effect of optical light on the generation and manipulation of microvortex flow named ‘twin opposing microvortex’ (TOMV) flow. This opto-electrohydrodynamic (OEHD) technique combines optical light, i.e. infrared (IR) laser (1064 nm), with non-uniform AC electric fields generated from a pair of indium tin oxide (ITO) electrodes. When the IR laser beam passes through the electric fields, a rapid and three-dimensional (3D) vortex flow is generated in a microchamber. When the laser beam passes through the electric fields, especially the exposed ITO electrode, the direction of the TOMV flow as well as its strength are controlled. With an AC signal of 107 kHz and various voltages below a peak-to-peak voltage of 10 V, laser power is varied up to 1.5 W and the path of a laser beam relative to the electrode (300 μm long and 16 μm wide) is manipulated. The maximum in-plane velocity outside the electrode region was obtained by micron-resolution particle image velocimetry (μPIV). When the laser beam passes through the left or right side of the lower electrode, the TOMV flow field rotates counterclockwise or clockwise, respectively. Applying optical light on an ITO electrode creates in situ and on-demand microvortex flow, which increases the feasibility of OEHD technique in various biological and chemical applications (e.g., mixing and delivering nanofluids in microfluidic devices).

A Computational Analysis of Turbocharger Compressor Flow Field with a Focus on Impeller Stall

Understanding the flow instabilities encountered by the turbocharger compressor is an important step toward improving its overall design for performance and efficiency. While an experimental study using Particle Image Velocimetry was previously conducted to examine the flow field at the inlet of the turbocharger compressor, the present work complements that effort by analyzing the flow structures leading to stall instability within the same impeller. Experimentally validated three-dimensional computational fluid dynamics predictions are carried out at three discrete mass flow rates, including 77 g/s (stable, maximum flow condition), 57 g/s (near peak efficiency), and 30 g/s (with strong reverse flow from the impeller) at a fixed rotational speed of 80,000 rpm. Large stationary stall cells were observed deep within the impeller at 30 g/s, occupying a significant portion of the blade passage near the shroud between the suction surface of the main blades and the pressure surface of the splitter blades. These stall cells are mainly created when a substantial portion of the inlet core flow is unable to follow the impeller’s axial to radial bend against the adverse pressure gradient and becomes entrained by the reverse flow and the tip leakage flow, giving rise to a region of low-momentum fluid in its wake. This phenomenon was observed to a lesser extent at 57 g/s and was completely absent at 77 g/s. On the other hand, the inducer rotating stall was found to be most dominant at 57 g/s. The entrainment of the tip leakage flow by the core flow moving into the impeller, leading to the generation of an unstable, wavy shear layer at the inducer plane, was instrumental in the generation of rotating stall. The present analyses provide a detailed characterization of both stationary and rotating stall cells and demonstrate the physics behind their formation, as well as their effect on compressor efficiency. The study also characterizes the entropy generation within the impeller under different operating conditions. While at 77 g/s, the entropy generation is mostly concentrated near the shroud of the impeller with the core flow being almost isentropic, at 30 g/s, there is a significant increase in the area within the blade passage that shows elevated entropy production. The tip leakage flow, its interaction with the blades and the core forward flow, and the reverse flow within the impeller are found to be the major sources of irreversibilities.

Drug resistant pancreatic cancer cells exhibit altered biophysical interactions with stromal fibroblasts in imaging studies of 3D co-culture models.

Interactions between tumor and stromal cells are well known to play a prominent roles in progression of pancreatic ductal adenocarcinoma (PDAC). As knowledge of stromal crosstalk in PDAC has evolved, it has become clear that cancer associated fibroblasts can play both tumor promoting and tumor suppressive roles through a combination of paracrine crosstalk and juxtacrine interactions involving direct physical contact. Another major contributor to dismal survival statistics for PDAC is development of resistance to chemotherapy drugs. Though less is known about how the acquisition of chemoresistance impacts upon tumor-stromal crosstalk. Here, we use 3D co-culture geometries to recapitulate juxtacrine interactions between epithelial and stromal cells. In particular, extracellular matrix (ECM) overlay cultures in which stromal cells (pancreatic stellate cells, or normal human fibroblasts) are placed adjacent to PDAC cells (PANC1), result in direct heterotypic cell adhesions accompanied by dramatic fibroblast contractility which leads to highly condensed macroscopic multicellular aggregates as detected using particle image velocimetry (PIV) analysis to quantify cell velocities over the course of time lapse movie sequences. To investigate how drug resistance impacts these juxtacrine interactions we contrast cultures in which PANC1 are substituted with a drug resistant subline (PANC1-OR) previously established in our lab. We find that heterotypic cell-cell interactions are highly suppressed in drug-resistant cells relative to the parental PANC1 cells. To investigate further we conduct RNA-seq and bioinformatics analysis to identify differential gene expression in PANC1 and PANC1-OR, which shows that negative regulation of cell adhesion molecules, consistent with increased epithelial mesenchymal transition (EMT), is also consistent with loss of hetrotypic cell-cell contact necessary for the contractile behavior observed in drug naïve cultures. Overall these findings elucidate the role of drug-resistance in inhibiting an avenue of stromal crosstalk which is associated with tumor suppression and also help to establish cell culture conditions useful for further mechanistic investigation.