Although particle dampers are characterized by a wide damping frequency band, flexible arrangement, and applicability to harsh environments, they have three shortcomings: their performance is difficult to simulate, insufficient research has been conducted on the damping effect they exert on multi-story structures, and there are no methods for evaluating their performance. Therefore, the aim of this research was to develop a Multiple Unidirectional Single-Particle Damper (MUSPD). Firstly, the simulation element of the MUSPD was established by analyzing its damping mechanism, following which the values of the parameters in the element were determined. Secondly, a series of shaking table tests were performed to investigate the damping effect of the MUSPD and demonstrate the accuracy of the simulation. Thirdly, the optimal layout of the MUSPD was proposed based on an analysis of the relationship between particle mass, particle movement distance, and damping effect. Fourthly, a dynamic method for evaluating the damping effect of the MUSPD was proposed. Finally, the rationality and feasibility of the optimal layout scheme and the method for evaluating the MUSPD in multi-story structures were verified by a case analysis. The test results demonstrated that the damping rate of MUSPD for displacement peaks in multi-story structures reached 21.40 %, with a root mean square damping rate of 36.50 %. Additionally, for acceleration peaks, MUSPD achieved a damping rate of 25.12 %, with a root mean square damping rate of 38.10 %. MUSPD exhibited a significant damping effect on multi-story structures. The comparison between test results and simulation results showed that the simulation element of the MUSPD exhibited good accuracy; Simulation analysis results revealed that the optimal layout scheme and dynamic evaluation method were reasonable and effective.