We investigate the Peres-Horodecki positive partial transpose (PPT) criterion in the context of conserved quantities and derive a condition of in- separability for a composite bipartite system depending only on the dimen- sions of its subsystems, which leads to a bi-linear entanglement witness for the two qubit system. A separability inequality using generalized Schrodinger- Robertson uncertainty relation taking suitable operators, has been derived, which proves to be stronger than the bi-linear entanglement witness operator. In the case of mixed density matrices, it identically distinguishes the separable and non separable Werner states.