Partial Overlap Research Articles

Counterpointing Care

An intricate lace-like net of chitin, acids and enzymes dwell in the interstices of life and nonlife, between plants, animals, biomass and the atmosphere, working in and through others; always dismantling, repairing and remaking matter. These are the ecologies of mycelial networks and fungal bodies that have given rise to biospherical life as we know it. Now, on the eve of planetary environmental collapse, calls to care for these vital fungal relationships are growing louder. What does it mean to care for something so obfuscated yet ubiquitous, something often regarded as abject yet gastronomic in some cultures, something that moulds human life but is yet abstract and unsympathetic to it? We argue that care in all its complexity and generality is in crisis because it is based on certain anthropocentric assumptions that do not account for the difference and indifference of fungi. Drawing on three discrete performative acts, we explore the possibilities of an abstract care when fungi is engaged as an ‘actor’ or agent, in which normative performances of ecological care are troubled. Through Wendy Wheeler’s biosemiotics, Jakob von Uexkull’s Umwelt and Merlin Sheldrake’s vital mycology, we ruminate on how these nets, cast wide into human and nonhuman guts, genes, brains and affects, themselves perform abstractions that are not mechanical but rather driven by semaphore and semiosis. We focus on the biosemiotics of eating (with) fungi as a performative act for care for more-than-human and human entanglements. As Jane Bennett suggests, a partial material overlap occurs through eating that is ontologically constitutive, moulding a kind of radical care through the communicative exchange of bodies and matter. Taking seriously these interdependent abstractions by mycelium’s sympoetic and holobiontic entanglements defines intuition as non-cognitive but sympathetic (after Bergson), enacting unequal and perhaps ‘care-less’ collaborations.

Precipitation Method for Determination of Carbon and Oxygen Isotopes to Detect Groundwater Contamination Near a Municipal Landfill

The paper emphasizes the importance of isotope studies as a unique tool for detecting groundwater contamination with the landfill leachate. The aim of the study was to present an additional and useful method for detecting groundwater contamination, based on stable isotope analysis. The proposed method relies on the interpretation of measured δ13CDIC and δ18O levels (in precipitated carbonates during preparation of a water sample). According to this method, two zones with different isotope composition of groundwater were identified: the first zone with natural groundwater and with low δ13CDIC levels (from −20.6 to −12.4‰) and high δ18O levels (from −13.6 to −8.0‰), and the second zone with leachate-contaminated groundwater rich in δ13CDIC (from −10.9 to + 3.6‰) and high level of δ18O (from −9.8 to −7.1‰). Measuring the isotopic composition of oxygen alone, is insufficient to delimit the contaminated zone due a partial overlap of natural levels with those of the contaminated with groundwater leachates. Determination of δ13CDIC and δ18O in the landfill leachate-contaminated water can provide an effective tool to detect groundwater contamination near municipal landfills, and it can help to minimize the number of samples collected for the analysis of conventional parameters. This proven method may offer an easy-to-use solution for detecting groundwater contamination.

Multimodal assessment of the GABA system in patients with fragile-X syndrome and neurofibromatosis of type 1

Fragile-X syndrome (FXS) and Neurofibromatosis of type 1 (NF-1) are two monogenic disorders sharing neurobehavioral symptoms and pathophysiological mechanisms. Namely, preclinical models of both conditions show overactivity of the mTOR signaling pathway as well as GABAergic alterations. However, despite its potential clinical relevance for these disorders, the GABAergic system has not been systematically studied in humans. In the present study, we used an extensive transcranial magnetic stimulation (TMS) assessment battery in combination with magnetic resonance spectroscopy (MRS) to provide a comprehensive picture of the main inhibitory neurotransmitter system in patients with FXS and NF1. Forty-three participants took part in the TMS session (15 FXS, 10 NF1, 18 controls) and 36 in the MRS session (11 FXS, 14 NF1, 11 controls). Results show that, in comparison to healthy control participants, individuals with FXS and NF1 display lower GABA concentration levels as measured with MRS. TMS result show that FXS patients present increased GABAB-mediated inhibition compared to controls and NF1 patients, and that GABAA-mediated intracortical inhibition was associated with increased excitability specifically in the FXS groups. In line with previous reports, correlational analyses between MRS and TMS measures did not show significant relationships between GABA-related metrics, but several TMS measures correlated with glutamate+glutamine (Glx) levels assessed with MRS. Overall, these results suggest a partial overlap in neurophysiological alterations involving the GABA system in NF1 and FXS, and support the hypothesis that MRS and TMS assess different aspects of the neurotransmitter systems.

Enhanced red-UC luminescence through Ce3+ co-doping in NaBiF4:Yb3+/Ho3+(Er3+)/Ce3+ phosphors prepared by ultrafast coprecipitation approach

Series of Yb3+/Ho3+(Er3+)/Ce3+ co-doped NaBiF4 phosphors were synthesized through an ultrafast co-precipitation reaction technique at room temperature. The effect of the Ce3+ ions on the crystal structure and upconversion (UC) luminescence properties of the studied samples were investigated in detail. FTIR and XPS demonstrated the pre-formation of NaBiF4 and the introduction of Yb3+, Ho3+, Er3+ and Ce3+ all as dopants in the host materials. Under 980 nm excitation, NaBiF4:Yb3+,Ho3+(Er3+),Ce3+performed the characteristic emission of the activator ion, and the introduction of Ce3+ did not change the emission wavelengths, only the relative intensities. Due to partial good energy overlap when 2F7/2 Ce3+ manifold is populated, raising Ce3+ ions concentration enhanced the red UC emission versus green UC emission but also lead to significant decrease in the average lifetimes of all monitored emissions for Ho3+ and Er3+. These lifetime decreases are explained by the energy loss in non-radiative pathways after the introduction of Ce3+. In addition, the green to yellow color emission change through addition of Ce3+ was explored in NaBiF4: Yb3+,Ho3+,Ce3+ to propose a novel application in two-level anti-counterfeiting.

Image histogram decomposition method for particle sizing – A numerical simulation study

Scanning probe microscopy is a useful tool in nanoscience. The effective application of nanotechnologies in various fields requires a knowledge of the characteristic attributes of nanoparticles such as shape, dimensions and statistical distribution, and a wide spectrum of experimental and theoretical methods based on various principles have been developed to determine these characteristics. Image histograms offer a global overview of the characteristics of an image. Their shape can encode specific statistical properties of displayed objects such as the distribution function in the case of similar and scalable objects. The model of height histogram presented here proposes a method which solves the long-term problem of processing images of extremely dense particle distributions. The method is based on the principle of the superposition of histograms of individual particles whose topographic surface is described by a parametric model. The resulting height histogram is defined by a convolution of the model of the particle histogram with the distribution function of particle size, with this construction forming the basis of the regression model. The parameters of the distribution function can be obtained via the optimization of the model. The method has been tested on artificially generated configurations of particles of various shapes and size distributions. Each of these configurations creates a topographic surface which is transformed into an image, and the heights obtained from the image allow a histogram to be calculated. Firstly, various configurations of particles are simulated without the presence of any disruptive influences. Next, several experimental effects are evaluated separately (for example, the background, particle shape irregularity and particle overlap). The decomposition of the histogram by the regression model on artificially generated images shows the robustness of the method with respect to particle density, partial horizontal overlap, randomly generated backgrounds and random fluctuations in particle shape. However, the method is sensitive to uniform changes in particle shape, a factor which limits its use to particles with known parametric models of their shape which allow the means of their parameters to be estimated.

Robust real-world point cloud registration by inlier detection

Real-world point cloud registration is challenging because of large outliers in correspondence search. The mixture variations, such as partial overlap, noise and cross sources, are the root cause of these large outliers. Existing methods face challenges in effectively removing the large outliers. We propose a novel coarse-to-fine framework to remove the outliers by detecting the accurate inlier correspondences. Specifically, our coarse module predicts the top-K accurate correspondences. The coarse module is trained by jointly leveraging global and local structured information. Then, our refinement module checks the correspondences further using our proposed novel higher-order filter, which enables the structure conformity of correspondences to improve the quality of inlier correspondences. The final transformation matrix is calculated by using the refined inlier correspondences. Furthermore, a new cross-source point cloud dataset is proposed to further demonstrate the robustness in real-world point clouds. Experimental results demonstrate that our algorithm achieves the state-of-the-art accuracy on both indoor and outdoor, same-source and newly proposed cross-source real-world point clouds.

Bulk photovoltaic effect in partial overlap MoSe[formula omitted]-WSe[formula omitted] van der Waals heterostructures: An ab initio quantum transport study

Two-dimensional (2-D) materials, especially transition metal dichalcogenides (TMDCs), have attracted great attention due to their unique properties and the vast possibility they offer when combined into van der Waals heterostructures (vdWHs), for example in optoelectronic applications. Although various photodetectors based on TMDC vdWHs have been successfully synthesized, an ab initio simulation framework dedicated to such devices is still missing. In this work, we investigate two photodetector designs based on MoSe2-WSe2 vdWHs through a recently developed approach combining density functional theory and quantum transport calculations. Geometries with a partial and total TMDC overlap are considered, with a p-doped (n-doped) left (right) extension and an intrinsic region in the middle. We show that the partial overlap structure supports a non-zero photo-current, even without a p–n junction, and gives rise to the bulk photovoltaic effect (BPVE). The present results provide an easy-to-fabricate guideline for engineering the BPVE in the TMDC vdWHs.

Investigation of Measurement Accuracy of Bridge Deformation Using UAV-Based Oblique Photography Technique.

This paper investigates the measurement accuracy of unmanned aerial vehicle-based oblique photography (UAVOP) in bridge deformation identifications. A simply supported concrete beam model was selected and measured using the UAVOP technique. The influences of several parameters, such as overall flight altitude (h), local shooting distance (d), partial image overlap (λ), and arrangement of control points, on the quality of the reconstructed three-dimensional (3D) beam model, were presented and discussed. Experimental results indicated that the quality of the reconstructed 3D model was significantly improved by the fusion overall-partial flight routes (FR), of which the reconstructed model quality was 46.7% higher than those with the single flight route (SR). Despite the minimal impact of overall flight altitude, the reconstructed model quality prominently varied with the local shooting distance, partial image overlap, and control points arrangement. As the d decreased from 12 m to 8 m, the model quality was improved by 48.2%, and an improvement of 42.5% was also achieved by increasing the λ from 70% to 80%. The reconstructed model quality of UAVOP with the global-plane control points was 78.4% and 38.4%, respectively, higher than those with the linear and regional control points. Furthermore, an optimized scheme of UAVOP with control points in global-plane arrangement and FR (h = 50 m, d = 8 m, and λ = 80%) was recommended. A comparison between the results measured by the UAVOP and the total station showed maximum identification errors of 1.3 mm. The study’s outcomes are expected to serve as potential references for future applications of UAVOP in bridge measurements.

Comparative study between radiofrequency-induced and muscimol-induced inhibition of cultured networks of cortical neuron.

Previous studies have shown that spontaneously active cultured networks of cortical neuron grown planar microelectrode arrays are sensitive to radiofrequency (RF) fields and exhibit an inhibitory response more pronounced as the exposure time and power increase. To better understand the mechanism behind the observed effects, we aimed at identifying similarities and differences between the inhibitory effect of RF fields (continuous wave, 1800 MHz) to the γ-aminobutyric acid type A (GABAA) receptor agonist muscimol (MU). Inhibition of the network bursting activity in response to RF exposure became apparent at an SAR level of 28.6 W/kg and co-occurred with an elevation of the culture medium temperature of ~1°C. Exposure to RF fields preferentially inhibits bursting over spiking activity and exerts fewer constraints on neural network bursting synchrony, differentiating it from a pharmacological inhibition with MU. Network rebound excitation, a phenomenon relying on the intrinsic properties of cortical neurons, was observed following the removal of tonic hyperpolarization after washout of MU but not in response to cessation of RF exposure. This implies that hyperpolarization is not the main driving force mediating the inhibitory effects of RF fields. At the level of single neurons, network inhibition induced by MU and RF fields occurred with reduced action potential (AP) half-width. As changes in AP waveform strongly influence efficacy of synaptic transmission, the narrowing effect on AP seen under RF exposure might contribute to reducing network bursting activity. By pointing only to a partial overlap between the inhibitory hallmarks of these two forms of inhibition, our data suggest that the inhibitory mechanisms of the action of RF fields differ from the ones mediated by the activation of GABAA receptors.

Serotonin modulation in the male Aedes aegypti ear influences hearing.

Male Aedes aegypti (Ae. aegypti) mosquitoes rely on hearing to identify conspecific females for mating, with the male attraction to the sound of flying females (“phonotaxis”) an important behavior in the initial courtship stage. Hearing thus represents a promising target for novel methods of mosquito control, and hearing behaviors (such as male phonotaxis) can be targeted via the use of sound traps. These traps unfortunately have proven to be relatively ineffective during field deployment. Shifting the target from hearing behavior to hearing function could therefore offer a novel method of interfering with Ae. aegypti mating. Numerous neurotransmitters, including serotonin (5-hydroxytryptamine, or 5-HT) and octopamine, are expressed in the male ear, with modulation of the latter proven to influence the mechanical responses of the ear to sound. The effect of serotonin modulation however remains underexplored despite its significant role in determining many key behaviors and biological processes of animals. Here we investigated the influence of serotonin on the Ae. aegypti hearing function and behaviors. Using immunohistochemistry, we found significant expression of serotonin in the male and female Ae. aegypti ears. In the male ear, presynaptic sites identified via antibody labelling showed only partial overlap with serotonin. Next, we used RT-qPCR to identify and quantify the expression levels of three different serotonin receptor families (5-HT1, 5-HT2, and 5-HT7) in the mosquito heads and ears. Although all receptors were identified in the ears of both sexes, those from the 5-HT7 family were significantly more expressed in the ears relative to the heads. We then thoracically injected serotonin-related compounds into the mosquitoes and found a significant, reversible effect of serotonin exposure on the male ear mechanical tuning frequency. Finally, oral administration of a serotonin-synthesis inhibitor altered male phonotaxis. The mosquito serotonergic system and its receptors thus represent interesting targets for novel methods of mosquito, and thus disease, control.

Vertebrate extracellular matrix protein hemicentin-1 interacts physically and genetically with basement membrane protein nidogen-2

Hemicentins are large proteins of the extracellular matrix that belong to the fibulin family and play pivotal roles during development and homeostasis of a variety of invertebrate and vertebrate tissues. However, bona fide interaction partners of hemicentins have not been described as yet. Here, applying surface plasmon resonance spectroscopy and co-immunoprecipitation, we identify the basement membrane protein nidogen-2 (NID2) as a binding partner of mouse and zebrafish hemicentin-1 (HMCN1), in line with the formerly described essential role of mouse HMCN1 in basement membrane integrity. We show that HMCN1 binds to the same protein domain of NID2 (G3) as formerly shown for laminins, but with an approximately 3.5-fold lower affinity and in a competitive manner. Furthermore, immunofluorescence and immunogold labeling revealed that HMCN1/Hmcn1 is localized close to basement membranes and in partial overlap with NID2/Nid2a in different tissues of mouse and zebrafish. Genetic knockout and antisense-mediated knockdown studies in zebrafish further show that loss of Nid2a leads to similar defects in fin fold morphogenesis as the loss of Laminin-α5 (Lama5) or Hmcn1. Finally, combined partial loss-of-function studies indicated that nid2a genetically interacts with both hmcn1 and lama5. Together, these findings suggest that despite their mutually exclusive physical binding, hemicentins, nidogens, and laminins tightly cooperate and support each other during formation, maintenance, and function of basement membranes to confer tissue linkage.

Glutathione-capped gold nanoclusters as near-infrared-emitting efficient contrast agents for confocal fluorescence imaging of tissue-mimicking phantoms.

An innovative research has been conducted focused on demonstrating the ability of novel dual-emissive glutathione-stabilized gold nanoclusters (GSH-AuNCs) to perform bright near-infrared (NIR)-emitting contrast agents inside tissue-mimicking agarose-phantoms via two complementary confocal fluorescence imaging techniques. First, using a new and fast microwave-assisted approach, we synthesized photostable dual-emitting GSH-AuNCs with an average size of 3.2 ± 0.4 nm and NIR emission quantum yield of 9.9%. Steady-state fluorescence measurements coupled with fluorescence lifetime imaging microscopy (FLIM) assays performed on lyophilized GSH-AuNCs revealed that the obtained GSH-AuNCs exhibit PL emissions at 610nm (red PL) and, respectively, 800nm (NIR PL) in both solution and powder solid-state. Time-resolved fluorescence measurements showed that the two PL components are characterized by average lifetimes of 407ns (red PL) and 1821ns (NIR PL), respectively. Additionally, due to a partial overlap between the red PL and the absorption of the NIR PL, an energy transfer between the two coexisting emissive centers was discovered and confirmed via steady-state and time-resolved fluorescence measurements. Furthermore, the FLIM analysis performed on powder GSH-AuNCs under 640nm, an excitation more suitable for bioimaging applications, revealed a homogeneous and photostable NIR PL signal from GSH-AuNCs. Finally, the ability of GSH-AuNCs to operate as reliable NIR-emitting contrast agents inside tissue-mimicking agarose-phantoms was demonstrated here for the first time via complementary FLIM and re-scan confocal fluorescence imaging techniques. In consequence, GSH-AuNCs show great promise for future in vivo imaging applications via confocal fluorescence microscopy.

Tumor Antigenicity and a Pre-Existing Adaptive Immune Response in Advanced BRAF Mutant Colorectal Cancers.

Simple SummaryBRAF mutant metastatic CRCs (BRAF-mCRCs) are considered a unique clinical entity characterized by a dismal prognosis and that do not respond efficiently to both standard chemotherapy and to orally selective inhibitors of BRAFV600E. In this study, the gene expression profiles of 89 immunotherapy-naïve BRAF-CRCs were generated using the PanCancer IO 360 gene expression panel to improve the knowledge of the mechanisms involved in tumor-suppressive immune functions in BRAF-mCRCs. A significant fraction of BRAF-mCRCs shows a hot/inflamed profile and may be potential candidates for responding to immunotherapy. Only a partial overlap between these hot signatures and the presence of microsatellite instability (MSI) was observed, demonstrating that MSI tumors showed a not differential expression of MHC Class I antigen presentation pathway compared with microsatellite-stable tumors. The analysis of gene expression profiles is a promising strategy both for immune profiling of primary tumors before any treatment and for following the evolution of metastatic disease during therapy.The main hypothesis of this study is that gene expression profiles (GEPs) integrating both tumor antigenicity and a pre-existing adaptive immune response can be used to generate distinct immune-related signatures of BRAF mutant colorectal cancers (BRAF-CRCs) to identify actionable biomarkers predicting response to immunotherapy. GEPs of 89 immunotherapy-naïve BRAF-CRCs were generated using the Pan-Cancer IO 360 gene expression panel and the NanoString nCounter platform and were correlated with microsatellite instability (MSI) status and with CD8+ tumor-infiltrating lymphocyte (TIL) content. Hot/inflamed profiles were found in 52% of all cases, and high scores of Tumor Inflammation Signature were observed in 42% of the metastatic BRAF-CRCs. A subset of MSI tumors showed a cold profile. Antigen Processing Machinery (APM) signature was not differentially expressed in MSI tumors compared with MSS cases. By contrast, the APM signature was significantly upregulated in CD8+ BRAF-CRCs versus CD8− tumors. Our study demonstrates that a significant fraction of BRAF-CRCs may be a candidate for immunotherapy and that the simultaneous analysis of MSI status and CD8+ TIL content increases accuracy in identifying patients who can potentially benefit from immune checkpoint inhibitors. GEPs may be very useful in expanding the spectrum of patients with BRAF-CRCs who can benefit from immune checkpoint blockade.

The International Settlement of Disputes Under the (Draft) CAI

Abstract The EU-China Comprehensive Agreement on Investment (CAI) establishes a sophisticated inter-State dispute settlement mechanism, directly inspired by the World Trade Organization (WTO) and adapted to a bilateral context, as is also found in recent preferential trade agreements. The novelty here lies in its extension to the field of investment entry and investment facilitation. Some variations in detail are also important. Because of partial material overlap, it is necessary to consider how choice of forum is organized between CAI procedures and those under WTO or other agreements, and to clarify the possible interplay with bilateral investment treaties previously concluded by China with EU Member States. The attention given in CAI to specific procedures for sustainable development and subsidies is also notable. In sum, the solutions adopted show a willingness on the part of the two powers to exert a certain influence on developments in international investment law, without upsetting the methods of dispute resolution.

The beauty of language structure: A single-case fMRI study of palindrome creation

Humans seem to be inherently driven to engage in wordplay. An example is the creation of palindromes – sentences that read the same backward and forward. This activity can be framed as a curiosity-driven behaviour, in which individuals seek for information that serves no direct purpose and in the absence of external rewards. In this fMRI case study, an experienced palindrome creator was scanned while he generated palindromes with different levels of difficulty. Palindrome creation was alternated with resting periods and with a working memory task, both serving as control conditions. Relative to resting, palindrome creation recruited frontal domain-specific language networks and fronto-parietal domain-general networks. The comparison with the working memory task evidenced partial overlap with the multiple-demand cortex, which participates in solving a variety of cognitively challenging tasks. Intriguingly, greater difficulty during palindrome creation differentially activated the right frontopolar cortex (BA 10), a region that was also linked to palindrome resolution. The latter is consistent with exploratory behaviour – in this case, with seeking new but interdependent linguistic segments within a complex internal model (i.e., a palindromic structure)– and bears resemblance with brain substrates sustaining hard logical reasoning, altogether pointing to a commonplace for curiosity in discovering new and complex relations.

Comprehensive Molecular Profiling of Oncocytic Salivary Gland Malignancies.

Oncocytic histologic features can be seen in a variety of salivary gland carcinomas. We performed a comprehensive molecular profiling of 15 salivary gland malignancies with oncocytic features (diagnosed as oncocytic carcinoma, carcinoma NOS with oncocytic features, or salivary duct carcinoma with oncocytic features). We reveal multiple novel molecular alterations that have not been previously described in other salivary gland malignancies, including, but not limited to, KEL amplification (13.3%, 2/15), PARP1 amplification (13.3%, 2/15), and EPHB4 amplification (13.3%, 2/15). Alterations in KMT2C (13.3%, 2/15), ERBB3 (13.3%, 2/15), CTNNA1 (13.3%, 2/15), and SMAD4 (20%, 3/15) were also found in this series and have been reported in other salivary gland malignancies. Alterations that have been reported in salivary duct carcinoma were also identified, including TP53 (40%, 6/15) , ERBB2 mutations (13.3%, 2/15) , ERBB2 amplification (13.3%, 2/15), PIK3CA (26.7%, 4/15) , PTEN (20%, 3/15), BRCA2 (20%, 3/15), BRAF (20%, 3/15), CDKN2A/B (20%, 3/15), CDH1 (13.3%, 2/15), and HRAS (13.3%, 2/15). Oncocytic salivary gland malignancies are a molecularly heterogenous group of tumors with partial overlap with salivary duct carcinoma subtypes.

Localization of chemical synapses and modulatory release sites in the cardiac ganglion of the crab, Cancer borealis.

The crustacean cardiac ganglion (CG) comprises nine neurons that provide rhythmic drive to the heart. The CG is the direct target of multiple modulators. Synapsin-like immunoreactivity was found clustered around the somata of the large cells (LC) and in a neuropil at the anterior branch of the CG trunk of Cancer borealis. This implicates the soma as a key site of synaptic integration, an unusual configuration in invertebrates. Proctolin is an excitatory neuromodulator of the CG, and proctolin-like immunoreactivity exhibited partial overlap with putative chemical synapses near the LCs and at the neuropil. A proctolin-like projection was also found in a pair of excitatory nerves entering the CG. GABA-like immunoreactivity was nearly completely colocalized with chemical synapses near the LCs but absent at the anterior branch neuropil. GABA-like projections were found in a pair of inhibitory nerves entering the CG. C. borealis Allatostatin B1 (CbASTB), red pigment concentrating hormone, and FLRFamide-like immunoreactivity each had a unique pattern of staining and co-localization with putative chemical synapses. These results provide morphological evidence that synaptic input is integrated at LC somata in the CG. Our findings provide a topographical organization for some of the multiple inhibitory and excitatory modulators that alter the rhythmic output of this semi-autonomous motor circuit.

First comprehensive analysis of Aedes aegypti bionomics during an arbovirus outbreak in west Africa: Dengue in Ouagadougou, Burkina Faso, 2016-2017.

Dengue's emergence in West Africa was typified by the Burkina Faso outbreaks in 2016 and 2017, the nation's largest to date. In both years, we undertook three-month surveys of Aedes populations in or near the capital city Ouagadougou, where the outbreaks were centered. In 1200LG (urban), Tabtenga (peri-urban) and Goundry (rural) localities, we collected indoor and outdoor resting mosquito adults, characterized larval habitats and containers producing pupae and reared immature stages to adulthood in the laboratory for identification. All mosquito adults were identified morphologically. Host species (from which bloodmeals were taken) were identified by PCR. Generalized mixed models were used to investigate relationships between adult or larval densities and multiple explanatory variables. From samples in 1,780 houses, adult Ae. aegypti were significantly more abundant in the two urban localities (Tabtenga and 1200 LG) in both years than in the rural site (Goundry), where Anopheles spp. were far more common. Results from adult collections indicated a highly exophilic and anthropophilic (>90% bloodmeals of human origin) vector population, but with a relatively high proportion of bloodfed females caught inside houses. Habitats producing most pupae were waste tires (37% of total pupae), animal troughs (44%) and large water barrels (30%). While Stegomyia indices were not reliable indicators of adult mosquito abundance, shared influences on adult and immature stage densities included rainfall and container water level, collection month and container type/purpose. Spatial analysis showed autocorrelation of densities, with a partial overlap in adult and immature stage hotspots. Results provide an evidence base for the selection of appropriate vector control methods to minimize the risk, frequency and magnitude of future outbreaks in Ouagadougou. An integrated strategy combining community-driven practices, waste disposal and insecticide-based interventions is proposed. The prospects for developing a regional approach to arbovirus control in West Africa or across Africa are discussed.

Bayesian iterative closest point for mobile robot localization

Accurate localization of a robot in a known environment is a fundamental capability for successfully performing path planning, manipulation, and grasping tasks. Particle filters, also known as Monte Carlo localization (MCL), are a commonly used method to determine the robot’s pose within its environment. For ground robots, noisy wheel odometry readings are typically used as a motion model to predict the vehicle’s location. Such a motion model requires tuning of various parameters based on terrain and robot type. However, such an ego-motion estimation is not always available for all platforms. Scan matching using the iterative closest point (ICP) algorithm is a popular alternative approach, providing ego-motion estimates for localization. Iterative closest point computes a point estimate of the transformation between two poses given point clouds captured at these locations. Being a point estimate method, ICP does not deal with the uncertainties in the scan alignment process, which may arise due to sensor noise, partial overlap, or the existence of multiple solutions. Another challenge for ICP is the high computational cost required to align two large point clouds, limiting its applicability to less dynamic problems. In this paper, we address these challenges by leveraging recent advances in probabilistic inference. Specifically, we first address the run-time issue and propose SGD-ICP, which employs stochastic gradient descent (SGD) to solve the optimization problem of ICP. Next, we leverage SGD-ICP to obtain a distribution over transformations and propose a Markov Chain Monte Carlo method using stochastic gradient Langevin dynamics (SGLD) updates. Our ICP variant, termed Bayesian-ICP, is a full Bayesian solution to the problem. To demonstrate the benefits of Bayesian-ICP for mobile robotic applications, we propose an adaptive motion model employing Bayesian-ICP to produce proposal distributions for Monte Carlo Localization. Experiments using both Kinect and 3D LiDAR data show that our proposed SGD-ICP method achieves the same solution quality as standard ICP while being significantly more efficient. We then demonstrate empirically that Bayesian-ICP can produce accurate distributions over pose transformations and is fast enough for online applications. Finally, using Bayesian-ICP as a motion model alleviates the need to tune the motion model parameters from odometry, resulting in better-calibrated localization uncertainty.

Сверхмощное вспышечное явление мазера водяного пара в протозвездной системе IRAS 16293-2422

Based on long-term monitoring of the water vapor maser at 22.2 GHz from early 2019 to March 2021, the most powerful flare event in the entire history of observations was recorded in the protostellar system IRAS 16293-2422 at a velocity of about –1.5 km s–1, lasting of about two years. The maser emission came from the largest structure of maser spots, the high density of which created their partial overlap in time. The total number of individual flares of the water maser reached ten. Due to the high detail of the data obtained, recorded at intervals of 1–2 days, new unique results were obtained. The existence of such a complex configuration of emitting maser spots with very similar radial velocities located on the observer’s line of sight was confirmed for the first time. It was possible to establish that the masers of powerful flares were in an un-saturated state due to the cascade pumping of several masers on the observer’s line of sight. New important parameters of water masers have been obtained, and an assumption has been made about their localization in the gas-dust structure IRAS 16293-2422.