Parsley Seedlings Research Articles

Metabolism and Degradation of Nicotinic Acid in Parsley (Petroselinum hortense) Cell Suspension Cultures and Seedlings

Abstract Application of [6-14C]-or [7-14C]nicotinic acid to parsley cell suspension cultures led to the accumulation of labelled nicotinamide mononucleotide, nicotinamide adenine dinucleotide, nicotinamide N-riboside, nicotinamide and nicotinic acid, indicating the operation of the pyridine nucleotide cycle in these cells. As main conjugates, nicotinic acid N-glucoside and nicotinic acid glucose ester were found. For nicotinic acid degradation the following sequence is suggested: nicotinic acid → 6-hydroxynicotinic acid → 2,5-dihydroxypyridine → a C4/C3 unit of unknown structure → CO2. In aseptically grown parsley seedlings nicotinic acid is also degraded to CO2