Parijoto Fruit Research Articles

Ekstraksi berbantu gelombang ultrasonik dan fotodegradasi senyawa antosianin pada tangkai parijoto (Medinilla speciosa Blume)

Parijoto is an endemic plant widely found on the slopes of Mount Muria, Kudus Regency, Central Java. The local community often consumes parijoto fresh fruit and processes parijoto fruit such as syrup, dodol, candy, and tea. So far, the Parijoto stalk, which weighs 18.19% of the total weight of the parijoto stalk and fruit, has not been widely used, but it has great potential as a source of anthocyanin. Anthocyanin compounds have low stability to temperature and light. For this reason, extraction with a relatively low temperature and faster than conventional methods is required, one of which is Ultrasonic-assisted extraction. Ultrasonic waves produce cavitation bubbles, facilitating solvent penetration so that cell bioactive components can be extracted more effectively. This study aimed to determine the effect of ultrasonic wave-assisted extraction time of parijoto stalk anthocyanin and examine the effect of various light exposure intensities and exposure times (photodegradation) on Parijoto stalk anthocyanin extract. Ultrasonic-assisted extraction of parijoto stalk anthocyanins used a completely randomized design (CRD) with extraction time (5, 10, 15, 20, and 25 minutes) and three treatment repetitions. In photodegradation using a factorial complete randomized design with the treatment of light exposure intensity (2037 lux, 4259 lux, 6482 lux, and 9259 lux) and exposure time (6, 12, 18, and 24 hours). The results showed that the longer the extraction, up to 20 minutes, the total anthocyanin levels, redness (a*), and yellowness (b*) increased while lightness (L*) decreased. The higher the light intensity of the fluorescent lamp and the longer the light exposure, the lower the total anthocyanin content with a lower half-life. Ultrasonic-assisted extraction have proven to be quite effective for the anthocyanin extraction but need to attention with time and light exposure intensity.

Fortification of local fruit parijoto on soy yogurt drink and economy analysis

Background: The food and beverage industry is a leading industry that is the government's focus in the Industrial Revolution 4.0. Culinary is the most popular economic sub-sector.. As a food product from biotechnology, yoghurt always manages to occupy a global market share. Yoghurt is proven to maintain the digestive system. Therefore innovation is needed, especially regarding materials, to boost product competitiveness. Animal milk substrate can be substituted with soy milk which is cheap but rich in protein. The addition of natural fortification is projected to improve product quality. Parijoto fruit (Medinilla speciosa) typical of the slopes of Mount Muria, Colo Village, Kab. Kudus was chosen as a fortificant because it is rich in antioxidants and antibacterial pathogens. At the same time, this adds to the usability and economic value of Parijoto, which is still low so far. Method: The research was conducted experimentally, referring to previous research methods. The formulation design was varied regarding adding Parijoto fruit extract to 100 ml of the product. Findings: From the results of the organoleptic test, the F1 formula (addition of 5 ml of Parijoto fruit extract) received the best acceptance from the panellists, with a preference level of 4.89. The product characteristics are liquid texture, alcoholic sweet taste, cream colour, and the aroma of Parijoto fruit tends to be faint. Conclusion:Parijoto Yogurt has advantages in terms of locality, price and nutrition. However, further research is needed based more collaboration to increase this product's health and economic impact local society. Novelty/Originality of this article: This study creates new functional food products and opens up opportunities to revitalize local economies and conserve biodiversity.

Merancang Bisnis Sampo Alami dari Ekstrak Buah Parijoto melalui Analisis Pasar

The use of synthetic products often causes skin problems such as irritation, itching, allergies and erythema. This causes the use of natural products to become an alternative for dealing with hair problems. In entering the natural cosmetics business, market analysis is required. This research aims to analyze the market so that it can determine consumer interest and market opportunities for natural shampoo from parijoto fruit extract. Market opportunity analysis was carried out using the PAM TAM SAM SOM method, Google Trends analysis, competitor analysis, and descriptive analysis of survey results of 52 potential consumers via Google Form. The results of the analysis show that the product has a very large and profitable opportunity in the market, is superior to several competitors, and the dry/powder form of shampoo is more popular than the liquid shampoo form, 82.7% of respondents are interested in using powder shampoo and 96.2% are interested use natural shampoo.

Formulation of nanoemulsion parijoto fruit extract (Medinilla Speciosa) with variation of tweens stabilizers.

Nanotechnology has substantial potential for development due to its ability to modify surface characteristics and particle size, facilitating enhanced absorption of functional food compounds and controlled release of active substances to mitigate adverse effects. Nanoemulsion, a stable colloidal system formed by blending oil, emulsifier, and water, was identified as nanotechnology with promising applications. However, investigations into the impact of surfactants on characteristic nanoemulsions need to be more varied. This research gap necessitated further exploration in the advancement of nanotechnology-based foods. The parijoto fruit (Medinilla speciosa), an indigenous plant species in Indonesia, has yet to undergo extensive scrutiny for its potential use as a functional and nutraceutical food. Anthocyanins, a principal compound in the parijoto fruit, had exhibited efficacy in reducing the risk of cardiovascular disease diabetes, demonstrating anti-inflammatory and antioxidant properties. This study aimed to investigate the characteristics of nanoemulsion formulations derived from parijoto fruit extract and to evaluate an optimum condition with various tween surfactants. The findings from this investigation could furnish valuable insights for the further advancement of anthocyanin nanoemulsions from parijoto fruit extract. The results comprised the characterization of nanoemulsion particle size, polydispersity index, ζ-potential, conductivity, pH, and viscosity. Through mathematical modeling and statistical methods, RSM optimizes nanoemulsion by examining the relationships and interactions between independent and response variables. Furthermore, the characterization of nanoemulsion encompassed ζ-potential, polydispersity, particle size, conductivity, pH, and viscosity. Elevated surfactant concentrations resulted in diminished particle sizes and more uniform size distribution, albeit reaching a plateau where surfactant aggregation and micelle formation ensued. Increased concentrations of surfactant type, concentration, and parijoto extract impacted the physical characteristics of nanoparticle size and polydispersity. The optimal process conditions for nanoemulsion consisting of the type of Tween used are Tween 80, Tween concentration of 12%, and parijoto fruit extract concentration of 7.5%, yielding a desirability value of 0.74, categorizing it as moderate.

The Potential of Parijoto Fruit Extract Fraction (Medinilla speciosa Blume) as Antibiofilm against Staphylococcus aureus Bacteria

Parijoto fruit extract (Medinilla speciosa Blume) contains flavonoids, saponins, tannins, and terpenoids and has antibacterial potential in inhibiting the growth of Staphylococcus aureus bacteria. S. aureus bacteria are bacteria that are capable of forming biofilms. This research aims to determine the potential of extracts and fractions (water, ethyl acetate, and n-hexane) in inhibiting the formation and degradation of S. aureus biofilms. Parijoto fruit was extracted using the maceration method with 96% ethanol solvent. The extract was fractionated using a liquid-liquid partition technique with water, ethyl acetate, and n-hexane as solvents. The antibiofilm activity test against the S. aureus bacteria ATCC 25923 was carried out by treatment inhibiting biofilm formation and biofilm degradation. The method is to read absorbance using a microplate reader with a wavelength of 595 nm. Optical density results to calculate IC50 and EC50 values. The results showed that the extract and fractions (water, ethyl acetate, and n-hexane) had the potential to inhibit the formation and degradation of S. aureus bacterial biofilms. The IC50 of biofilm inhibition in the extract, water fraction, ethyl acetate, and n-hexane against S. aureus bacteria was 2.51; 1.03; 1.10; 1.52 mg/ml. The EC50 value of the biofilm degradation test on the extract, water fraction, ethyl acetate, and n-hexane against S. aureus bacteria was 1.03; 1.17; 1.37; 1.88 mg/ml. The most active fraction of parijoto fruit (Medinilla specosa Blume) in inhibiting and degrading S. aureus bacterial biofilms is the water fraction.

An activity of flavonoid derived compounds from Medinilla speciosa as anti-hyperpigmentation against tyrosinase proteins with in silico methods

Parijoto (Medinilla speciosa), a typical plant from Indonesia, contains flavonoid compounds as antioxidants. It is able to depigment skin by inhibiting tyrosinase activity during melanogenesis, or melanin formation. Melanogenesis occurs through UV light exposure; it stimulates ROS production and triggers the formation of free radicals and melanogenesis. If not prevented, it will have negative impacts on health. Currently, there is no further research regarding the existence of flavonoid compound derivatives found in parijoto fruit. The aim of this research is to initially identify potential flavonoid derivative compounds as tyrosinase inhibitors using in silico methods. The results can be used as an initial reference for making products based on natural ingredients with minimal side effects. This research uses a bioinformatics approach with the molecular docking method of ligands towards proteins. The stages in this research include: downloading and preparing receptors and ligands; docking with Autodock Vina; visualization of results with Biovia Discovery Studio; data analysis; and discussion. The analysis is carried out by looking at the affinity energy values and complex conformation between the receptor-ligand. The results show flavonoid derivative compounds have the potential to act as tyrosinase inhibitors, as proven by compounds interactions with the active site of tyrosinase to produce the amino acid residues phenylalanine, proline, asparagine, arginine, and histamine. Produce respective affinity energy values, namely -8.9; -7.7; -7.6; -7.5; -7.5; and 7.4 kcal/mol for chalcone, catechin, flavonol, flavanol, flavone, and flavonone compounds. Meanwhile, the comparison compound used is kojic acid, with an affinity energy of -5.5 kcal/mol.

Developing Local MSMEs with Indonesian Socio-Culture Service Learning for Muria's Processed Parijoto Fruit

The concept of entrepreneurship empowerment was explored in depth to highlight its multifaceted nature and its role in fostering economic growth and cultivating a positive community mindset, which in turn spurred the development of essential infrastructure contributing to community empowerment. Service Learning, as a strategy for community entrepreneurship empowerment in the Indonesian culture, was enacted through the collaboration of faculty and university students who were acknowledged for their capacity to deliver authentic learning experiences. It, thereby, facilitated the acquisition of both soft and hard skills. This initiative exemplified the coordinated efforts among universities, such as the local MSME (Micro, Small, and Medium Enterprises) Community of processed Parijoto fruit, and its various partners. The execution of this community service program, which was done in Muria, comprised six primary phases: surveying, conducting interviews, gathering data, preparing materials, engaging in focus group discussions, formulating action plans, and conducting evaluations and reflections. The outcomes encompassed the analysis of laboratory products, educational sessions, mentoring activities, and the creation of educational resources. While the assessment of processing parijoto fruit yielded positive impacts from the endeavor, it was found to have a need to optimize support from businesses to sustain a conducive environment for MSMEs. Such entrepreneurship training initiatives have demonstrated efficacy in empowering communities and generating further prospects for individuals residing in rural areas.

Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Etanol Buah Parijoto (Medinilla speciosa Blume) dengan Metode Spektrofotometri UV-Vis

Parijoto (Medinilla speciosa) has a wide range of pharmacological activities due to its secondary metabolite content, especially flavonoid, and phenolic compounds. Flavonoid and phenolic compounds have a major role in human health with their activities as antioxidants, anti-inflammatories, antimicrobials, etc. This study aimed to determine the total levels of phenolic and flavonoid compounds from the ethanol extract of Parijoto fruit. Parijoto fruit is obtained from Bandungan, Semarang Regency, Central Java. Extraction was carried out by maceration method with 70% ethanol solvent then concentrated using a rotary evaporator. The total phenolic and flavonoid content amount was determined using the colorimetric method with Folin-ciocalteau complex reagent on phenolic with gallic acid as the reference standard and AlCl3 complex reagent on flavonoids with quercetin as the reference standard. Absorbance was measured using a UV-Vis spectrophotometer. The results showed that the color of the viscous extract of Parijoto fruit was dark brown with a yield of 6.67% w/w. The total phenolic content of the ethanol extract of Parijoto fruit was 21.67 µgGAE/g extract and the total flavonoid content was 9.21 µg QE/g extract.

Hepatoprotective Effect of Parijoto Fruit Extract (Medinilla speciosa Blume) on Male Mice Fed with High-Fat Diet

This study aims to determine the effectiveness of parijoto fruit extract (Medinilla speciosa Blume) in improving the condition of fatty liver in male balb/c mice (Mus musculus L.) fed with a high-fat diet. Thirty male balb/c mice weighing 20 to 30 g were randomly divided into six groups, i.e: 1) Standard feed and Carboxymethyl Cellulose Sodium or CMC-Na 0.5% (K1); 2) High-fat diet and CMC-Na 0.5% (K2); 3) High-fat diet and simvastatin 0.026 mg/day (K3); 4) High-fat diet and parijoto fruit extract 5.6 mg/20 g BW (P1); 5) High-fat diet and parijoto fruit extract 8.4 mg/20 g BW (P2); 6) High-fat diet and parijoto fruit extract 11.2 mg/20 g BW (P3). Standard feed, high-fat diet, simvastatin, and parijoto fruit extract were administered for 56 days. On the 57th day, the total of 30 mice were terminated and the livers were then removed for H&E staining histopathological slides. Data on the degree of fatty liver on histopathological slides were collected and analyzed using the Kruskal-Wallis test and followed by the Mann-Whitney test. The histopathological analysis showed Medinilla speciosa Blume extract at a dose of 5.6 mg/20 g BW in group P1 prevent the steatosis degree compared to high-fat feed mice in group K2 (p>0.05). Parijoto fruit extract could act as the potential treatment for fatty liver.

ARTIKEL REVIEW: STUDI FITOKIMIA DAN FARMAKOLOGI PARIJOTO (Medinilla magnifica)

Health problems are one of the most complex problems, the sanitation process in improving health with various uses of plants in the State of Indonesia. A country that will be rich in natural resources. As a form of serious treatment that improves health from various diseases in Indonesia, called by utilizing Parijoto fruit (Medinilla magnifica) by utilizing the active substances contained in the fruit in the form of flavonoids, tannins, saponins and alkaloids through in vitro and in vitro tests. In vitro which has important properties in the treatment process in Parijoto fruit, extract is made by maceration using 96% ethanol solvent for several properties such as antioxidant, antidiabetic, antiacne, anticholesterol, antihyperlipidemia, antibacterial, anti-breast cancer and toxicity to improve public health in Indonesia. In this review, primary data searches are conducted online, in the form of national and international journals, books and ebooks. The results obtained from several journals and other sources can be seen in various kinds of pharmacological activities of the active substances contained in parijoto (Medinilla magnifica)

Copigmentation of Anthocyanin Extract from Parijoto Fruit (Medinilla speciosa) and Its Stability at Different Temperatures and Heating Durations

Parijoto fruit (Medinilla speciosa) has a red color and contains anthocyanins. Anthocyanins have low stability due to the effect of heating. Copigmentation can be applied to improve the stability of anthocyanins. This research aims to study the effect of the anthocyanin extract from parijoto fruit ratio and copigment on anthocyanin concentration and color stability during heating at different temperatures and durations. Anthocyanin copigmentation using tannic acid in a ratio of 1:20 and 1:40 with control without copigmentation (1:0) showed that increasing the tannic acid copigment would increase anthocyanin concentrations and reduce anthocyanin losses due to increased temperature and heating time. The use of 1:40 tannic acid copigment increased anthocyanin concentration by 38%. In addition, increasing the concentration of tannic acid increased the ability of anthocyanins to maintain color retention during heating and increased temperatures. The use of tannic acid at a ratio of 1:40 only decreased color retention by 16%, compared to control (54%) after heating at 75ºC heating for 120 minutes. Copigmentation with tannic acid up to a ratio of 1:40 was able to maintain the stability of the lightness (L*), the reddish value (a*), and the yellowish value (b*) of the anthocyanins. This study showed that this system is potential for food coloring application in the food industry.

Physical comparison of parijoto fruit from hydroponic farming with parijoto fruit from natural plantations

One of the advantages of hydroponic systems is that plants can be cultivated under controlled environmental conditions. The parijoto fruit plant has also been successfully cultivated with a bucket hydroponic system. This fruit has been shown to have a high antioxidant capacity. In this study wanted to compare the physical characteristics of parijoto fruit grown with hydroponic farming, with parijoto fruit obtained from the garden naturally. The method used in this study is to buy the seeds of the parijoto plant. It later developed it in the hydroponic farming, Dutch Bucket System, DFT (Deep Flow Technique). After the plant is fruited and ripe, parijoto fruit is picked and subjected to physical testing. Similar tests were imposed on samples of parijoto fruit obtained from natural plantations. The results of the test are further compared. Based on physical testing data obtained the conclusion that the diameter of parijoto fruit produced by hydroponic agriculture has a smaller diameter than the diameter of parijoto fruit produced by natural plantations. Lighter fruit weight, almost the same purple color, have the same moisture content, the same sugar content, higher pH value, as well as lower mineral levels.

Effect of Methanol Extract, Methanol Fraction, and N-Hexane Fraction of Parijoto Fruit (Medinilla speciosa) on Seminal Parameters and Testicular Weight of Male Sprague Dawley Rats

Infertility occurs in 2 million couples or 17% of couples who are married more than 2 years but are not pregnant or have signs of pregnancy. In couples who do not have children, 50% of male infertility factors are caused by abnormalities in semen. Empirically, parijoto fruits (Medinilla speciosa Blume) are used by the people of Kudus, Central Java, Indonesia to increase fertility. The purpose of this research was to find out the effect of methanol extract, methanol fraction, and n-hexane fraction of parijoto fruit (Medinilla speciosa) on seminal parameters and testicular weight of male Sprague Dawley rats. This research used 36 two-month-old Sprague Dawley rats with 200-300 gram of body weight which were divided into 6 groups. Group 1 (normal); groups 2, 3, and 4, used parijoto fruit methanol extract at 100mg/kgBW, 250mg/kgBW, and 500mg/kgBW doses respectively; groups 5 and 6 used methanol fraction and n-hexane fraction of parijoto fruit at 500 mg/kgBW dose respectively for 14 days. Rats were dissected and had examinations on sperm motility, morphology, abd testicular weight. The data were analyzed using Kruskal Wallis and Mann Whitney. The results found that groups 1, 2, 3, 4, 5 and 6 had mean spermatozoa motility (%) that were 56.5±2.43, 72.5±6.89, 77.6±12.99, 83.3±7.53, 84.7±3.98, and 74.2±11.58, mean spermatozoa morphology (%) that were 95.5±3.67, 95±2.76, 92.6±5.13, 96.5±3.27, 94.3±4.37, and 93.2±6.11, and mean testicular weight (gram) that were 1.08±0.10, 0.90±0.14, 0.98±0.10, 1.18±0.21, 1.28±0.43, and 1.02±0.13. There were significant differences between the normal group and all treatments (P <0.05) on spermatozoa motility. There was no significant difference on spermatozoa morphology and testicular weight. Based on the results, it can be concluded that methanol extract, methanol fraction, and n-hexane fraction of parijoto fruit can increase spermatozoa motility of male Sprague Dawley rats.

Pest and Disease Identification in Parijoto Plant (Medinilla speciosa blume) at Nglurah Tawangmangu

Parijoto (Medinilla speciosa blume) is a tropical plant that has purplish pink fruit often called Asian grape. In Indonesia, parijoto plants are very well known as ornamental plants that have various benefits, from parijoto fruit can be used as anti-inflammatory drugs so that ornamental plants are widely cultivated by the community, especially lovers of ornamental plants and ornamental plant cultivation in Nglurah. Medinilla speciosa blume is very suitable in Indonesia, which has a tropical climate, but the high rainfall in Indonesia in the rainy season should get more attention in caring for it. This makes parijoto plants susceptible to several types of diseases and pests that can damage the beauty of the Medinilla speciosa blume plant. However, the lack of experts in this field makes it very difficult for plant lovers and the cultivation community of Medinilla speciosa blume in Nglurah to solve the problem of diseases and pests that attack the Medinilla speciosa blume plant. The purpose of this study was to determine pests and diseases on Medinilla speciosa blume. This research was conducted in Nglurah Village Rt 01 Rw 02 Tawangmangu for five days on 04 – 08 September 2021. This research uses observation and interview methods. Data were analyzed by qualitative descriptive. This study used 10 samples of the observed Medinilla speciosa blume plant. The tools used are cellphone cameras and stationery. The variables to be observed are pests and diseases. The results of this study found 4 pests and 2 diseases on Medinilla speciosa blume including Fussarium oxysporum, Cercospora sp and Pestalotia sp, mealybugs, caterpillars, snails, grasshoppers. For this reason, the solution for treating parijoto plants to avoid pests and diseases requires extra care such as paying attention to soil moisture, giving appropriate fertilizers, and giving vitamins so that parijoto plants can grow well.

Antioxidant Activity and Cytotoxic Potential of Parijoto (Medinilla speciosa (Reinw ex BL)) Fruit Fractions on HeLa Cell Line

Parijoto (Medinilla speciosa (Reinw ex BL)) is one of the Indonesian indigenous plants widely used as traditional medicine. A previous study showed that ethanol and methanol extracts of Parijoto fruit could inhibit T47D breast cancer cells. This study explores the antioxidant and cytotoxic activities of Parijoto fruit extract and fractions on HeLa cell line. The fruits were extracted using ethanol 70% and fractionated by hexane and ethyl acetate. Furthermore, the fraction was analyzed for secondary phytochemical metabolite content using thin-layer chromatography and staining reagents. The antioxidant and cytotoxic activities were determined using the DPPH scavenging assay and the MTT assay, respectively. The ethanol extract and fraction contained flavonoid and tannin compounds. Ethanol extract, ethanol fraction, and ethyl acetate fraction of Parijoto fruit had an antioxidant activity with IC50 values of 77.3, 88.64, and 46.61 μg/mL, respectively. Ethyl acetate fraction showed the highest activity on HeLa cells with an IC50 value of 45.57 μg/mL compared to ethanol extract, ethanol fraction and n-hexane fraction with an IC50 value of 233.43, 700.75, and 534.30 μg/mL, respectively. Based on these results, the ethyl acetate fraction of Parijoto fruit had the potency to be explored further to elucidate their cytotoxicity mechanism in HeLa cells.Keywords: antioxidant activity, cytotoxicity, Medinilla speciosa, Parijoto fruit fractions.

Use of Edible Film Incorporated with Parijoto Fruit Extract (Medinilla speciosa Blume) to Inhibit Microbiological and Oxidative Damages of Sausages

Background and Objective: Edible film is one of the solutions for food packaging that carry antimicrobial and antioxidant compounds, usually found in Medinilla speciosa fruits. The objective of this study was to assess effects of Medinilla speciosa fruit extract on physical, chemical and bioactivity of edible films as well as effects of coating on sausage quality during storage. Material and Methods: The edible film included 2% w w-1 chitosan, 2% w w-1 sorbitol and Medinilla speciosa fruit extract. Variations of Medinilla speciosa extract included 0, 2.5, 5 and 10% (w w-1), while the storage temperature included 4 and 27 oC. Seven parameters of edible film characteristics were assessed, including tensile strength, elongation, water vapor permeability, antimicrobial and antioxidant activities, surface microstructure and Fourier-transform infrared response. Parameters assessed in storage treatment included total plate count, yeast mold count and thiobarbituric acid reactive substances. Data were analyzed using Kruskal-Wallis test. Organoleptic characteristics were analyzed using Friedman test and SPSS Software. Results and Conclusion: Results showed that the higher the concentration of Medinilla speciosa extract was, the higher the value of tensile strength, water vapor permeability and antioxidant activity and lower the elongation value were. The film control with 0% Medinilla speciosa extract was the only film that met Japanese standard for the water vapor permeability value, including 6.74 gm-2 h-1. Furthermore, shelf life of sausages coated with edible films revealed that the higher the concentration of Medinilla speciosa extract was, the lower the total plate count, yeast mold count and thiobarbituric acid reactive substances values were. Study demonstrated that the Medinilla speciosa extract edible film inhibited microbiological and oxidative damages. ​Oon Day 15 of storage, sausages coated with edible films with 10% Medinilla speciosa extract included lower total plate count, yeast mold count and thiobarbituric acid reactive substances values, respectively including 2.4 ±0.02 log CFU g-1, 1.3 ±0.08 log CFU g-1 and 13.38 ±0.22 mg malonaldehyde kg-1 sausage, compared to the control film. Organoleptic assessment showed no major differences in consumer acceptance. In conclusion, edible film with 10% Medinilla speciosa extract is the best physical, chemical and bioactivity film. Moreover, this film extends the sausage shelf life. Conflict of interest: The authors declare no conflict of interest.

Selectivity of Ethanol Extract of Parijoto (Medinilla speciosa) Fruit in HepG2, Widr, 4T1, and Vero cell lines

Background: Parijoto, one of the melastomaceae family, has been known to have cytotoxic activity in some cancer cell lines, such as HeLa, MCF-7, and T47D. Aims: We aim to know about the selectivity of ethanol extract of Parijoto fruit in cell line HepG2, WiDr, 4T1, and Vero. Cytotoxic was determined by MTT assay. Method: Extract was added in three serial concentration three serial concentrations (125 µg/mL–500 µg/mL), while the positive control doxorubicin gives in 2,5 µg/mL – 20 µg/mL for cancer cell and 40 µg/mL -100 µg/mL for Vero cell. Results: Results showed that ethanol extract of parijoto fruit gave low activity in HepG2 and Vero cell (IC50: 250 µg/mL) and moderate activity in WiDr and 4T1 (IC50: 81,58 µg/mL and 158,72 µg/mL). Conclusion: The highest selectivity index is given in WiDr cell (SI> 3) means that the ethanol extract of parijoto fruit is a promising cytotoxic agent for colorectal cancer therapy.

Application of parijoto (medinilla speciosa l.,) extract as body lotion

Parijoto fruit is among the potential plant that is potential for beauty products. Parijoto fruit extract composed of flavonoids as source of antioxidants that is useful for maintaining healthy skin. The purpose of this study was to determine the validity and feasibility of hand and body lotion enriched with parijoto fruit extract determined by sensory and preference tests. This study used construct validity. Data was collected through observation and documentation methods. The results showed that hand and body lotion enriched with parijoto fruit extract was declared feasible based on sensory tests with the lowest percentage of 83% and the highest of 92%. The preference test obtained the lowest percentage of 82% and the highest percentage of 97%. Hand and body lotion was declared very feasible based on sensory and preference tests. There is a need of assessment in the used formula in order to obtain the best formula for the production of hand and body lotion.

Antibacterial Activity of Parijoto (Medinilla speciosa Blume) Fruit Against Serratia marcescens and Staphylococcus aureus

Parijoto (Medinilla speciosa Blume) is a plant found in Mount Muria, Kudus, Central Java. The local community often uses its fruit to treat various diseases, as well as antibacterial, anti-inflammatory, antioxidant, and anti-cancer. This study aimed to determine the antibacterial activity of n-hexane, ethyl acetate and methanol extract from parijoto fruit against Staphyococcus aureus ATCC 29213 and Serratia marcescens clinical isolates. Gradual maceration of simplicial and antibacterial activity testing of the extracts was performed, followed by determining the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and comparative antibacterial activity most active extract against ampicillin and cefadroxil. The results showed that all extracts exhibited activity against S. aureus ATCC 29213, but only ethyl acetate and methanol extracts had activity against S. marcescens clinical isolates. The highest antibacterial activity was shown by the methanol extract, which was more effective against S. aureus ATCC 29213, having the largest inhibition diameter and lowest MIC and MBC values (6.25 and 12.5 mg/mL). The comparative analysis indicated that the methanol extract demonstrated lower antibacterial activity against S. aureus ATCC 29213 than cefadroxil (72,511: 1). This antibacterial activity is presumed to be generated from alkaloids, polyphenols, tannins, flavonoids, quinones and saponina in the extract.Keywords: Amoxicillin, Cefadroxil, MIC, MBC

SKRINING FLAVONOID EKSTRAK BUAH PARIJOTO (Medinilla speciosa Blume) ASAL KABUPATEN KUDUS DAN SEMARANG DENGAN PEMBANDING KUERSETIN DAN RUTIN

Parijoto fruit (Medinilla speciosa Blume) is an epiphytic shrub with a height of 0.45-1.2 meters. Parijoto fruit contains secondary metabolites of flavonoids, anthosionins, glycosides and others which have pharmacological effects as antioxidants, antidiabetic, anticancer, antibacterial, anti-inflammatory. Parijoto plant growth is influenced by temperature, humidity, rainfall and altitude where it grows. This study aims to determine the total flavonoid content in Parijoto fruit from Kudus and Semarang districts. This type of research is an experimental laboratory research using UV-Vis spectrophotometry method using quercetin and rutin as a comparison. The total flavonoids of Parijoto fruit extract from Kudus Regency and Semarang, respectively, with a quarcetin ratio of 81.60 mgQE/g and 76.48 mgQE/g, while with a routine comparison of 79.33 mgRE/g and 73, respectively. 06 mgRE/g. The total flavonoids of the two extracts showed quite strong results, but were significantly different as evidenced by the value of the paired sample t-test between the two extracts with a significance value (2-tailed) <0.05.