The aim of the study is to increase the production efficiency of the hatching eggs by using the Kamyshenskoye-75 sorghum grain in the layer diet. For research, four groups of laying hens were formed from parent stock of 60 ones each (control, experimental-1, experimental-2, experimental-3). The duration of the scientific and economic study was 52 weeks. The layer diet of the control group was provided by the pedigree producer JV «Svetly». In Layer di-et, grain maize was replaced by sorghum: the first group content – 25.0%; the second group – 50.0%; the third group – 100.0%. The conditions of keeping and feeding of laying hens met the requirements of the parent flock of Hisex Brown. On the basis of the conducted studies, it was established that the balance of nitrogen utilization de-pends on the dose of sorghum grain for experimental layer diet. With sorghum grain in a volume of 50.0% in the layer diet, the balance of nitrogen use was 53.14%, which is more than when using grain sorghum 25.0 and 100.0%, and also exceeds the indicator of the control group by 2.06%. The balance of use of calcium by the control group of laying hens is 0.17% less than in the group with 100% grain maize substitution for sorghum in mixed feed. 50.0% sorghum grain use in mixed feed increases phosphorus performance efficiency by 2.36%, lysine amino ac-ids and methionine compared with the control. Grain maize substitution in mixed feed for the layer hen diet for grain sorghum cultivar Kamyshinsky-75 in the amount of 50.0% increases the nutritional value of mineral and availability of amino acids lysine and methionine.