Understanding dynamic changes in urban land surface temperature (U-LST) is vital for improving urban thermal environment. Previous studies noted a close association between two/three-dimensional (2D/3D) urban form parameters (UFPs) and U-LST, but divergent conclusions emerged due to study scale and data spatiotemporal resolution. This study investigated the effect of 2D/3D UFPs on multi-temporal (hourly, diurnal, and annual) U-LSTs with 30-m spatial resolution, which is the first exploration of such dynamics at this fine scale. In addition, the influence of local climate zones (LCZs) was considered. Results showed that 2D/3D vegetation UFPs have the greatest summer cooling effect at 21:00, while 3D building UFP sky view factor (SVF) impacts minimum nighttime temperature more (5:00). The independently total explained variances (R2) of 2D UFPs (0.2–0.7) on seasonal average U-LSTs were higher than that of 3D UFPs (0.1–0.4) in most LCZs. LCZ 3 and LCZ 2 had the highest seasonal and daytime U-LST, respectively. Interestingly, SVF replaced normalized difference vegetation index as the dominant factor in LCZs 4–6 and LCZs A-C after summer. Findings of this study are useful for guiding urban planning, formulating urban heat island mitigation policies, and promoting sustainable development goals (SDGs 11, 13, 15) of cities and communities.