Background: Understanding genetic variability parameters, correlation analysis and employing multivariate techniques such as principal component analysis and diversity analysis are crucial for designing effective breeding programs and conserving germplasm in pigeonpea. Morphological studies, when combined with multivariate analysis, offer valuable insights into genetic diversity, aiding in the selection of diverse parental lines and identifying promising segregants in hybridization programs. Methods: The study utilized 155 diverse genotypes of pigeon pea, planted in a randomized complete block design with three replications during the kharif seasons of 2022-23 and 2023-24. Character association analysis, multivariate analysis like PCA and diversity assessment were utilized. Result: Principal component analysis was employed to assess variability, where the first two principal components explained 35.63% of the total variability, with traits such as DFF, DM, NSB and NPP contributing significantly to variance. Morphological genetic diversity was assessed using Mahalanobis D2 statistics and hierarchical clustering. Morphological diversity assessment grouped the 155 pigeon pea genotypes into 9 clusters, with cluster I being the largest (88 genotypes). Hierarchical clustering identified 6 distinct clusters, with cluster-I housing the highest number of genotypes (43). Interestingly, the study revealed non-consistent connections between morphological diversity assessments and the geographical origin of genotypes. Based on genetic D2 values, genotypes in clusters VI and VII, followed by clusters VI and VIII, were found to be the most genetically distant, suggesting their potential use in hybridization programs to create diverse progenies. Selecting desirable genotypes from different clusters based on their performance could lead to the development of superior recombinants through hybridization.
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