Parallel Plate Compression Research Articles

A Review of Viscosity Measurement Techniques for Semi-Solid Metal Fluids in Thixoforming Processes

This paper critically reviews two viscometry techniques: capillary viscometers and parallel plate compression (PPC) viscometers, which are used in the viscosity measurement of semi-solid metal (SSM) fluids for thixo routes. Capillary and PPC viscometers have emerged as valuable viscometers in the study of thixo routes of SSM, with insights into their unique flow behavior being explored. Thixoprocessing of SSM is particularly suitable for specific alloys, especially those with specific solidification properties such as eutectics. It is a method of thixoforming that uses a material's non-dendritic microstructure. The thixo method's viscosity measurement is challenging, requiring sample preparation, sophisticated equipment, precise temperature, and pressure control. Previous studies have lacked sufficient information on measurement methods, viscometers, and limitations to measuring viscosity for the thixo route of SSM. Hence, this study thoroughly examines the principles, advantages, limitations, and application areas of viscometry methods. Several factors influencing viscosity measurements, such as temperature, shear rate, and sample preparation, are discussed in detail. A comparative analysis is conducted to evaluate the performance and accuracy of capillary viscometers and parallel plate compression viscometers. This review will provide a comprehensive understanding of SSM's viscometry techniques and assist researchers and practitioners in selecting the most appropriate method for their specific applications in semi-solid metal processing and characterization.

Assessing the functional mechanical properties of polycaprolactone stents post-processed by ultrasonic strengthening

In this study, the ultrasonic strengthening process was performed on the stents fabricated by the fused deposition modeling method with polycaprolactone (PCL) material. Functional tests entailing three-point bending, parallel-plate compression and biodegradable tests were performed to evaluate the effect of the ultrasonic strengthening process on the PCL polymeric stents. Results displayed that the bending and compression stiffness ratio improved value by 2.4 and 1.37 times, respectively, more than evidence-based stents without ultrasonic strengthening. Also, records revealed that the ultrasonic strengthening process increases in polymer degradation time by up to 60 %. The dynamic mechanical properties of stents were improved after ultrasonic enhancement. Observation of the surface morphology by Scanning Electron Microscope showed a cavity's size reduction and Adhesion between layers in the cross-section were owned to effect on ultrasonic strengthening process.

Building a tissue: Mesenchymal and epithelial cell spheroids mechanical properties at micro- and nanoscale.

Cell transitions between the epithelial and mesenchymal phenotypes provide the regulated morphogenesis and regeneration throughout the ontogenesis. The tissue mechanics and mechanotransduction play an essential role in these processes. Cell spheroids reproduce the cell density of native tissues and represent simple building blocks for the tissue engineering purposes. The mechanical properties of mesenchymal and epithelial cells have been extensively studied in 2D monolayer cultures, but have not been sufficiently compared in spheroids. Here, we have simultaneously applied several techniques to assess the mechanical parameters of such spheroids. The local surface mechanical properties were measured by AFM, and the bulk properties were analyzed with parallel-plate compression, as well as by observing cut opening after microdissection. The comparison of the collected data allowed us to apply the model of a solid body with surface tension, and estimate the parameters of this model. We found an expectedly higher surface tension in mesenchymal spheroids, as well as a higher bulk modulus and relaxation time. The two latter parameters agree with the bulk poroelastic behavior of spheroids, and with the higher cell density and extracellular matrix content in mesenchymal spheroids. The higher tension of the surface layer cells in mesenchymal cell spheroids was also confirmed by the viscoelastic AFM characterization. The cell phenotype affected the self-organization during the spheroid formation, as well as the structure, biomechanical properties, and spreading of spheroids. The obtained results will contribute to a more detailed description of spheroid and tissue biomechanics, and will help in controlling the tissue regeneration and morphogenesis. STATEMENT OF SIGNIFICANCE: Spheroids are widely used as building blocks for scaffold-based and scaffold-free strategies in tissue engineering. In most studies, either the concept of a solid body or a liquid with surface tension was used to describe the biomechanical behavior of spheroids. Here, we have used a model which combines both aspects, a solid body with surface tension. The "solid" aspect was described as a visco-poroelastic material, affected by the liquid redistribution through the cells and ECM at the scale of the whole spheroid. A higher surface tension was found for mesenchymal spheroids than that for epithelial spheroids, observed as a higher stiffness of the spheroid surface, as well as a larger spontaneous opening of the cut edges after microdissection.

Biocompatible micro tweezers for 3D hydrogel organoid array mechanical characterization.

This study presents novel biocompatible Polydimethylsiloxane (PDMS)-based micromechanical tweezers (μTweezers) capable of the stiffness characterization and manipulation of hydrogel-based organoids. The system showed great potential for complementing established mechanical characterization methods such as Atomic Force Microscopy (AFM), parallel plate compression (PPC), and nanoindentation, while significantly reducing the volume of valuable hydrogels used for testing. We achieved a volume reduction of ~0.22 μl/sample using the μTweezers vs. ~157 μl/sample using the PPC, while targeting high-throughput measurement of widely adopted micro-mesoscale (a few hundred μm-1500 μm) 3D cell cultures. The μTweezers applied and measured nano-millinewton forces through cantilever’ deflection with high linearity and tunability for different applications; the assembly is compatible with typical inverted optical microscopes and fit on standard tissue culture Petri dishes, allowing mechanical compression characterization of arrayed 3D hydrogel-based organoids in a high throughput manner. The average achievable output per group was 40 tests per hour, where 20 organoids and 20 reference images in one 35 mm petri dish were tested, illustrating efficient productivity to match the increasing demand on 3D organoids’ applications. The changes in stiffness of collagen I hydrogel organoids in four conditions were measured, with ovarian cancer cells (SKOV3) or without (control). The Young’s modulus of the control group (Control—day 0, E = 407± 146, n = 4) measured by PPC was used as a reference modulus, where the relative elastic compressive modulus of the other groups based on the stiffness measurements was also calculated (control-day 0, E = 407 Pa), (SKOV3-day 0, E = 318 Pa), (control-day 5, E = 528 Pa), and (SKOV3-day 5, E = 376 Pa). The SKOV3-embedded hydrogel-based organoids had more shrinkage and lowered moduli on day 0 and day 5 than controls, consistently, while SKOV3 embedded organoids increased in stiffness in a similar trend to the collagen I control from day 0 to day 5. The proposed method can contribute to the biomedical, biochemical, and regenerative engineering fields, where bulk mechanical characterization is of interest. The μTweezers will also provide attractive design and application concepts to soft membrane-micro 3D robotics, sensors, and actuators.

Parallel-plate compression test for soft materials: confocal microscopy-assisted ferrule-top nanoindentation.

The parallel-plate compression test is one of the simplest ways to measure the mechanical properties of a material. In this test, the Young's modulus ( ) and the Poisson's ratio ( ) of the material are determined directly without applying any additional modelling and parameter fitting in the post-processing. This is, however, limited when dealing soft biological materials due to their inherent properties such as being inhomogeneous, microscopic, and overly compliant. By combining an interferometry-assisted parallel-plate compression system and a confocal microscope, we were able to overcome these limitations and measure the (315 52 Pa) and (0.210 0.043) of fixated and permeabilized bovine oocytes.

Numerical Modelling of Multicellular Spheroid Compression: Viscoelastic Fluid vs. Viscoelastic Solid

Parallel-plate compression of multicellular spheroids (MCSs) is a promising and popular technique to quantify the viscoelastic properties of living tissues. This work presents two different approaches to the simulation of the MCS compression based on viscoelastic solid and viscoelastic fluid models. The first one is the standard linear solid model implemented in ABAQUS/CAE. The second one is the new model for 3D viscoelastic free surface fluid flow, which combines the Oldroyd-B incompressible fluid model and the incompressible neo-Hookean solid model via incorporation of an additional elastic tensor and a dynamic equation for it. The simulation results indicate that either approach can be applied to model the MCS compression with reasonable accuracy. Future application of the viscoelastic free surface fluid model is the MCSs fusion highly-demanded in bioprinting.

Microstructure and rheological properties of a semisolid A356 alloy with erbium addition

The effects of erbium addition on the rheological properties and microstructure of a semisolid A356 alloy were studied. The semisolid slurries were prepared through the serpentine channel technique before they were thixoformed using parallel-plate compression with cylindrical discs. The grain and globule size decreases as the Er content increases, resulting in an improved and uniform distribution of spherical primary α-Al phase within the semisolid slurry. The addition of the Er modifies the grain morphology and size of the α-Al grains, resulting in a better and more uniform distribution of spherical primary α-Al phase within the semisolid slurry. As a result, rheocast quality index increases with the addition of Er, which is suitable for the thixoforming process. The A356 alloy without Er has the highest viscosity herein. The viscosity decreases, and the flow characteristics of the semisolid feedstock are expected to improve when Er is added as a result of the refinement of primary α-Al and modification of eutectic silicon. Furthermore, the refined semisolid A356 alloys with Er show a slightly larger fraction of high-angle grain boundaries compared to that for the unrefined alloy.

A Discrete Multi-Physics Model to Simulate Fluid Structure Interaction and Breakage of Capsules Filled with Liquid under Coaxial Load

This paper investigated the mechanical response (including breakage and release of the internal liquid) of single core–shell capsules under compression by means of discrete multi-physics. The model combined Smoothed Particle Hydrodynamics for modelling the fluid and the Lattice Spring Model for the elastic membrane. Thanks to the meshless nature of discrete multi-physics, the model can easily account for the fracture of the capsule’s shell and the interactions between the internal liquid and the solid shell. The simulations replicated a parallel plate compression test of a single core–shell capsule. The inputs of the model were the size of the capsule, the thickness of the shell, the geometry of the internal structure, the Young’s modulus of the shell material, and the fluid’s density and viscosity. The outputs of the model were the fracture type, the maximum force needed for the fracture, and the force–displacement curve. The data were validated by reproducing equivalent experimental tests in the laboratory. The simulations accurately reproduced the breakage of capsules with different mechanical properties. The proposed model can be used as a tool for designing capsules that, under stress, break and release their internal liquid at a specific time.

Relation between viscosity and flow characteristics in uni-directional CF/PA6 laminates near the melting point

In this study, the viscosity of unidirectional carbon fiber reinforced thermoplastic (CFRTP) laminates near their melting point was evaluated to discuss the relationship between the thermal fusion conditions and the flow of the fibers and matrix. The laminates were regarded as a fluid flowing in the direction perpendicular to the fibers. The microscopic flow of the fibers and matrix was observed during a parallel plate compression test conducted near the melting point. On the basis of this observation, the viscosity and flow properties were evaluated by the finite element method (FEM). The results of the parallel plate compression test showed that the test conditions and the decrease in the thickness of the laminates are related to the apparent viscosity of the unidirectional plate. In addition, cracks in the direction of the maximum shear stress were detected via in situ observation near the melting point of the matrix. The occurrence of this phenomenon depended on the apparent viscosity of the unidirectional laminates, and it was found that no cracks occurred when the apparent maximum viscosity of the unidirectional laminates was lower than a threshold.

Abstract 1915: In vivo prostate tumor tissue stiffness differs by tumor region and can be recapitulated in bioengineered prostate tumor tissues

Abstract Throughout the tumorigenic process, locational heterogeneities in tumor tissue microarchitecture develop as a result of aberrant angiogenesis and a subsequently induced oxygen and nutrient gradient within the three-dimensional (3D) mass. This phenomenon often results in differential tissue stiffness between the necrotic, quiescent, and proliferative tumor regions. In vitro, strong correlations have been found to exist between cell culture platform stiffness and acquired chemoresistance and varied drug response. Therefore, to accurately recapitulate the tumor microenvironment, biomimetic models must provide a mechanically similar scaffold. This study reports novel quantification of the in vivo prostate tumor stiffness and the ensuing development of tunable 3D bioengineered tumor tissue (BioTT) to successfully recapitulate in vivo mechanical cues in vitro. In vivo samples were generated by subcutaneously injecting Matrigel-suspended metastatic prostate cancer cells (PC-3) into the flank of athymic NCr nude mice. Resultant tumors (300 – 1,500 mm3) were excised from the murine host and geometrically dissected to provide samples from the tumor core, midpoint, and periphery. The Young’s modulus was quantified via parallel plate compression under physiological conditions. The 3D BioTT model is comprised of poly(ethylene glycol)-fibrinogen (PF) with varying amounts of excess poly(ethylene glycol) diacrylate (PEGDA) to modulate the mechanical properties of the scaffold. PC-3 cancer cells and BJ-5ta human fibroblasts were encapsulated within the covalently crosslinkable biomaterial and co-cultured for 29 days in vitro. Cell viability was assessed by LIVE/DEAD staining and cellular morphology was visualized with Hoechst 33342, Phalloidin, and the anti-fibroblast immunomarker, TE-7. Temporal variations in cell populations were quantified by flow cytometry and mechanical stiffness characterization was again performed by parallel plate compression. In vivo prostate cancer tumors presented a wide range of tissue stiffness heterogeneity (200 – 5,750 Pa), characterized by an increasing modulus with respect to locational progression from the core to the periphery (n = 48 per tumor region). The BioTT model successfully recapitulated the full tumor stiffness range through biomaterial composition modulation; the addition of excess PEGDA significantly stiffened the PF scaffold (p ≤ 0.05, n = 3). PC-3 and BJ-5ta cells survived the encapsulation process and remained viable throughout long-term co-culture. Visualization of the 3D cellular microenvironment revealed both cancer and stromal cells maintained characteristic morphology. In future studies, the BioTT will be extended to a microfluidic chip platform, thus augmenting the physiological relevancy of the model by incorporating dynamic shear conditions and the ability to monitor cancer cell metastasis. Citation Format: Nicole L. Habbit, Benjamin Anbiah, Luke S. Anderson, Joshita Suresh, Iman Hassani, Matthew Eggert, Shanese L. Jasper, Balabhaskar Prabhakarpandian, Robert D. Arnold, Elizabeth A. Lipke. In vivo prostate tumor tissue stiffness differs by tumor region and can be recapitulated in bioengineered prostate tumor tissues [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 1915.

Abstract 999: In vivo breast tumor stiffness and vascular drug delivery recapitulated in a microfluidic tumor-on-a-chip

Abstract Biomimetic tissue engineered microfluidic cancer models offer a higher degree of spatial, temporal and structural precision in controlling the physical parameters and component characteristics of the native tumor microenvironment (TME). Current models working to establish a biomimetic in vitro breast TME are limited by their ability to recapitulate various degrees of in vivo complexities and poor correlation of the diffusional gradients of oxygen, nutrients and anti-cancer drugs. To establish a model and address these challenges, we have used poly(ethylene glycol)-fibrinogen (PEG-Fb) as our biomimetic material to engineer 3D breast tumor tissues and recapitulate the mechanical stiffness of core, midpoint and peripherial zones of the native tumor in a vascularized microfluidic chip. To assess the mechanical stiffness, the in vivo breast tumor (MDA-MB-231 flank xenograft in Athymic nude mice) and engineered tumor constructs were subjected to parallel plate compression test using Cell Scale Microsquisher and the resulting force versus displacement data was acquired to calculate Young’s modulus. Tumor mimetic (“high perfusion chip” (HPC) and “low perfusion chip” (LPC), differ with respect to the vascular network surrounding their respective primary and secondary tumor compartments were used in this study. Breast cancer-associated endothelial cells (hBTEC) were seeded in the vascular network and allowed to form a lumen. Metastatic breast cancer cells MDA-MB-231/ human foreskin fibroblast BJ5ta (ATCC) cells were mixed with polymer precursor solution containing PEG-Fb and Eosin Y. The precursor was loaded into the primary tumor compartment and cross-linked for 2 minutes under visible light. Stiffness was modulated by adding poly(ethylene glycol) diacrylate (PEGDA) to the polymer precursor for recapitulating the different zones of the in vivo tumor. hBTEC media was perfused through the endothelial cell networks were continuously monitored for cell behavior and metastasis. In vivo breast tumor stiffness at core, midpoint and periphery was found to be within the range of the 3D engineered breast tumor tissues with time in culture through day 29. In the vascularized microfluidic chip, cell laden biomaterial was incorporated, the cancer cells were observed to undergo key events of the TME such as intravasation, circulating tumor cells in the endothelial vascular channel, adherence and migration to the secondary chamber resulting in metastasis. In the native TME there are regional differences in drug diffusion; TRITC dextran (4.4 kDa) was administered at a constant flow rate through the chips’ vascularized networks and found to have vascular network geometry and engineered tumor construct stiffness dependent differences in diffusion into the primary tumor chamber, mimicking the in vivo phenomena. Citation Format: Benjamin Anbiah, Iman Hassani, Nicole Habbit, Lani Jasper, Deborah Ramsay, Balabhaskar Prabhakarpandian, Robert Arnold, Elizabeth Lipke. In vivo breast tumor stiffness and vascular drug delivery recapitulated in a microfluidic tumor-on-a-chip [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2019; 2019 Mar 29-Apr 3; Atlanta, GA. Philadelphia (PA): AACR; Cancer Res 2019;79(13 Suppl):Abstract nr 999.

Mechanical Characterization of 3D Ovarian Cancer Nodules Using Brillouin Confocal Microscopy.

The mechanical interaction between cells and their microenvironment is emerging as an important determinant of cancer progression and sensitivity to treatment, including in ovarian cancer (OvCa). However, current technologies limit mechanical analysis in 3D culture systems. Brillouin Confocal Microscopy is an optical non-contact method to assess the mechanical properties of biological materials. Here, we validate the ability of this technology to assess the mechanical properties of 3D tumor nodules. OvCa cells were cultured in 3D using two established methods: (1) overlay cultures on Matrigel; (2) spheroids in ultra-low attachment plates. To alter the mechanical state of these tumors, nodules were immersed in PBS with varying levels of sucrose to induce osmotic stress. Next, nodule mechanical properties were measured by Brillouin microscopy and validated with standard stress-strain tests: Atomic Force Microscopy (AFM) and a parallel plate compression device (Microsquisher). Finally, the nodules were treated with a chemotherapeutic commonly used to manage OvCa, carboplatin, to determine treatment-induced effects on tumor mechanical properties. Brillouin microscopy allows mechanical analysis with limited penetration depth (~ 92µm for Matrigel method; ~ 54µm for low attachment method). Brillouin microscopy metrics displayed the same trends as the corresponding "gold-standard" Young's moduli measured with stress-strain methods when the osmolality of the medium was increased. Nodules treated with carboplatin showed a decrease in Brillouin frequency shift. This validation study paves the way to evaluate the mechanics of 3D nodules, with micron-scale three-dimensional resolution and without contact, thus extending the experimental possibilities.

COMBINED USE OF PARALLEL-PLATE COMPRESSION AND FINITE ELEMENT MODELING TO ANALYZE THE MECHANICAL PROPERTIES OF INTACT PORCINE LENS

The objective of this study is to explore the feasibility of a compression test for measuring mechanical properties of intact eye lenses using novel parallel plate compression equipment to compare the accuracy of implementing a classical Hertzian model and a newly proposed adjusted Hertzian model to calculate Young’s modulus from compression test results using finite element (FE) analysis. Parallel-plate compression tests were performed on porcine lenses. An axisymmetric FE model was developed to simulate the experimental process to evaluate the accuracy of using the classical Hertzian theory of contact mechanics as well as a newly proposed adjusted Hertzian theory model for calculating the equivalent Young’s modulus. By fitting the force-displacement relation obtained from FE simulations to both the classical and adjusted Hertzian theory model and comparing the calculated modulus to the input modulus of the FE model, the results demonstrated that the classical Hertzian theory model overestimated the Young’s modulus with a proportional error of over 10%. The adjusted Hertzian theory model produced results that are closer to original input values with error ratios all lower than 1.29%. Measurements of three porcine lenses from the parallel plate compression experiments were analyzed with resulting values of Young’s modulus of between 3.2[Formula: see text]kPa and 4.3[Formula: see text]kPa calculated. This study demonstrates that the adjusted Hertzian theory of contact mechanics can be applied in conjunction with the parallel-plate compression system to investigate the overall mechanical behavior of intact lenses.

3D skeletal muscle fascicle engineering is improved with TGF-β1 treatment of myogenic cells and their co-culture with myofibroblasts.

BackgroundSkeletal muscle wound healing is dependent on complex interactions between fibroblasts, myofibroblasts, myogenic cells, and cytokines, such as TGF-β1. This study sought to clarify the impact of TGF-β1 signaling on skeletal muscle cells and discern between the individual contributions of fibroblasts and myofibroblasts to myogenesis when in co-culture with myogenic cells. 3D tissue-engineered models were compared to equivalent 2D culture conditions to assess the efficacy of each culture model to predictively recapitulate the in vivo muscle environment.MethodsTGF-β1 treatment and mono-/co-cultures containing human dermal fibroblasts or myofibroblasts and C2C12 mouse myoblasts were assessed in 2D and 3D environments. Three culture systems were compared: cell monolayers grown on 2D dishes and 3D tissues prepared via a self-assembly method or collagen 1-based hydrogel biofabrication. qPCR identified gene expression changes during fibroblast to myofibroblast and myoblast differentiation between culture conditions. Changes to cell phenotype and tissue morphology were characterized via immunostaining for myosin heavy chain, procollagen, and α-smooth muscle actin. Tissue elastic moduli were measured with parallel plate compression and atomic force microscopy systems, and a slack test was employed to quantify differences in tissue architecture and integrity.ResultsTGF-β1 treatment improved myogenesis in 3D mono- and co-cultures containing muscle cells, but not in 2D. The 3D TGF-β1-treated co-culture containing myoblasts and myofibroblasts expressed the highest levels of myogenin and collagen 1, demonstrating a greater capacity to drive myogenesis than fibroblasts or TGF-β1-treatment in monocultures containing only myoblasts. These constructs possessed the greatest tissue stability, integrity, and muscle fiber organization, as demonstrated by their rapid and sustained shortening velocity during slack tests, and the highest Young’s modulus of 6.55 kPA, approximate half the stiffness of in situ muscle. Both self-assembled and hydrogel-based tissues yielded the most multinucleated, elongated, and aligned muscle fiber histology. In contrast, the equivalent 2D co-culture model treated with TGF-β1 completely lacked myotube formation through suppression of myogenin gene expression.DiscussionThese results show skeletal muscle regeneration can be promoted by treating myogenic cells with TGF-β1, and myofibroblasts are superior enhancers of myogenesis than fibroblasts. Critically, both TGF-β1 treatment and co-culturing skeletal muscle cells with myofibroblasts can serve as myogenesis accelerators across multiple tissue engineering platforms. Equivalent 2D culture systems cannot replicate these affects, however, highlighting a need to continually improve in vitro models for skeletal muscle development, discovery of therapeutics for muscle regeneration, and research and development of in vitro meat products.

Mechanical Characterization of APA Microcapsules by Parallel-Plate Compression

We produced microcapsules of alginate-poly(L)lysine-alginate (APA)with diameters on the order of 10 µm. To characterize their mechanical properties, we conducted an experiment on the parallel-plate compression of a microcapsule and modeled its deformation. In the modeling task, the microcapsule was assumed to be a spherical liquid-filled elastic membrane with negligible bending stiffness and permeability. The membrane thickness was estimated by applying Reissner's linear elastic theory to the experimental force-displacement relationship during loading in the small displacement region. The initial stretch of the membrane was taken into account; it was mainly caused by the osmotic pressure difference across the membrane. The initial stretch of the microcapsule was determined by fitting the calculated and experimental force-displacement relationships during loading at small to medium displacements. At large displacements, the calculated force was smaller than the experimentally measured force because of fluid permeation across the APA membrane. The calculated and experimentally imaged shapes of the deformed microcapsule were compared. The effects of varying the membrane thickness on the force-displacement and transmural pressure-displacement curves were shown, and the limitations of applying the present deformation model were examined.

EMPIRICAL STUDY OF THE CHARACTERISTICS OF AFZELIA AFRICANA SEED UNDER COMPRESSIVE LOADING

Afzelia Africana seeds were subjected to quasi-static parallel plate compression test, using Monsanto Tensometer. The loading orientations were horizontal, vertical and edge-to-edge loading positions at four different moisture content levels of 10.6%, 12.8%, 15.2% and 18.4% wet basis (wb). Slight variations were observed in the means of the physical characteristics at varying moisture content in the range of 10.6% to 18.4%. Loading position was shown to have significant effect on all the mechanical properties studied. The rupture force increased with increase in moisture content in horizontal and vertical loading but did not show consistent trend in variation when loaded on the edges. This value was 155.68N for vertical loading, 139.27 N for horizontal loading and 81.0 N for edge-to-edge loading. Hardness ranged from 6.97 to 6.92 N/mm for horizontal loading, 6.92 to 6.67N/mm for edge-loading and 6.99 to 6.93N/mm for vertical loading respectively in the moisture content range of 10.6 to 18.4% for all cases. Rupture energy varied from 104 to 416.33 N-mm on horizontal loading, 59.96to 241.82 on edge-loading and 190.125 to 419.171 Nmm on vertical loading respectively for the same range of moisture content. Toughness varied from 43.758 to 8.865 N-m/mm, 23.563 to 5.370 and 44.667 to 16.349 N-m/mm for horizontal loading, edge-edge orientation, and vertical loading respectively, for moisture content range of 10.6 to 18.4%.Modulusof Elasticity dropped from 35.657 to 28.133 N/mm2 for horizontal loading, from 51.443 to 14.59 for edge-to-edge loading and 38.087 to 41.04 N/mm2for vertical loading in the range of moisture content of 10.6 to 18.4% respectively. The data generated in this study can be applied when estimating the energy and force needed for shelling the seeds and in making useful suggestions on the loading condition that will demand the least energy for the operation and shelling. The physical properties are also useful in process design and analysis.  http://dx.doi.org/10.4314/njt.v36i3.42

The role of adhesion junctions in the biomechanical behaviour and osteogenic differentiation of 3D mesenchymal stem cell spheroids

Osteogenesis of mesenchymal stem cells (MSC) can be regulated by the mechanical environment. MSCs grown in 3D spheroids (mesenspheres) have preserved multi-lineage potential, improved differentiation efficiency, and exhibit enhanced osteogenic gene expression and matrix composition in comparison to MSCs grown in 2D culture. Within 3D mesenspheres, mechanical cues are primarily in the form of cell-cell contraction, mediated by adhesion junctions, and as such adhesion junctions are likely to play an important role in the osteogenic differentiation of mesenspheres. However the precise role of N- and OB-cadherin on the biomechanical behaviour of mesenspheres remains unknown. Here we have mechanically tested mesenspheres cultured in suspension using parallel plate compression to assess the influence of N-cadherin and OB-cadherin adhesion junctions on the viscoelastic properties of the mesenspheres during osteogenesis. Our results demonstrate that N-cadherin and OB-cadherin have different effects on mesensphere viscoelastic behaviour and osteogenesis. When OB-cadherin was silenced, the viscosity, initial and long term Young's moduli and actin stress fibre formation of the mesenspheres increased in comparison to N-cadherin silenced mesenspheres and mesenspheres treated with a scrambled siRNA (Scram) at day 2. Additionally, the increased viscoelastic material properties correlate with evidence of calcification at an earlier time point (day 7) of OB-cadherin silenced mesenspheres but not Scram. Interestingly, both N-cadherin and OB-cadherin silenced mesenspheres had higher BSP2 expression than Scram at day 14. Taken together, these results indicate that N-cadherin and OB-cadherin both influence mesensphere biomechanics and osteogenesis, but play different roles.

Understanding the requirements of self-expandable stents for heart valve replacement: Radial force, hoop force and equilibrium

A proper interpretation of the forces developed during stent crimping and deployment is of paramount importance for a better understanding of the requirements for successful heart valve replacement. The present study combines experimental and computational methods to assess the performance of a nitinol stent for tissue-engineered heart valve implantation. To validate the stent model, the mechanical response to parallel plate compression and radial crimping was evaluated experimentally. Finite element simulations showed good agreement with the experimental findings. The computational models were further used to determine the hoop force on the stent and radial force on a rigid tool during crimping and self-expansion. In addition, stent deployment against ovine and human pulmonary arteries was simulated to determine the hoop force on the stent-artery system and the equilibrium diameter for different degrees of oversizing.

Microstructure and Rheological Properties of Semi-Solid 7075 Slurries Using SEED Rheocasting Process

The SEED rheocasting process was used to produce semisolid slurries of high strength 7075 aluminum wrought alloys. The effects of the SEED processing parameters on the microstructure of semisolid slurries were studied. The impact of grain refinement on the grain morphology and size was investigated. The rheological properties of semisolid 7075 alloys were characterized at different solid fractions using a parallel plate compression viscometer. Results indicated that the grain refinement could greatly improve the globularity of α-Al grains and rheoformability. The relationships between viscosity and shear rate at different solid fractions in the transient state for both unrefined and refined semisolid slurries were evaluated and discussed.

Development of a Finite Element Method Model to Determine Mechanical Behavior of Pumpkin Seed

In food process simulation studies, geometrical characterization of agricultural produce is extremely difficult due to their irregular shapes. Further simplifications and assumptions can be applied to overcome this problem. Also, applying 3D scanners may easily and realistically lead to more accurate results from simulations. The aim of this work was to study this discrepancy in the obtained values of the elastic parameters of organic materials under a compression case using a finite element method and experimental data. The Zaria variety of pumpkin seed was chosen as a typical sample of an organic material. A 3D scanner, high speed camera, and simulations using a finite element method were used to investigate modulus of elasticity, force, energy, and stress distribution of the pumpkin seed under the condition of parallel plate compression. The results of a compression test were used as experimental analysis. The force/deformation curve generated by the finite element method modeling matched the curve from the experimental test remarkably well up to 0.9 mm combined deformation and showed that the finite element method can be used to describe accurately mechanical behavior of pumpkin seed under compression.