The aims of this study were molecular identification some of virulence genes in Escherichia coli isolated from milk of bovines with clinical mastitis. (n = 60) E. coli isolates from acute clinical mastitis were examined for detect the presence of the genes encodingshigatoxin1 (stx1), intimin (eaeA), cytotoxicnecrotizingfactor 2 (cnf2), aerobactin (iucD) and P fimberiae (pap). The majors finding in the PCR assays were: 8 isolates (13.33%) had at least one virulence gene, None of isolates contained the genes for stx1, eaeA , the most common gene in the examined isolate was iucD which was positive in 6 isolates (10%). One isolate (1.66%) was positive for both iucD and pap genes and one isolate (1.66%) for cnf2 gene. In this study similar to previous investigations indicated that prevalence virulence genes in E. coli isolated of bovine mastitis is deferent. The results of this investigate similar to previous studies indicated none of the potential virulence genes or specific pathotype was observed in E. coli isolates from bovine clinical mastitis. DOI: http://dx.doi.org/10.3126/ijls.v8i2.10219 International Journal of Life Sciences Vol.8(2): 2014; 1-3