Feed acidifiers are used in animal diets for the prevention of proliferation of intestinal pathogenic microorganisms and resulting gastrointestinal digestive disorders. These additives, containing organic acids, have also been found to improve productivity and feed efficiency in poultry. There is information about correlation between digestive enzymes’ activity and intestinal microbiota of meattype chickens. However, the exact mechanism of the beneficial impact of organic acids on the digestion system still remains understudied. The paper presents the results of experiments conducted on Hisex White chicken with chronic fistulae of the main pancreatic duct, fed on a diet supplemented with an acidifier containing 2-furoic acid. No significant effect of this acidifier was found on the digestive pancreatic function. The dynamics analysis showed that the chickens’ secretion rate of pancreatic juice after postprandial 30 minutes dropped by over two times in the testing period when using acidifiers in their diet. After 150 minutes this rate was lower by 27.3% compared to the control group, which corresponds the neurochemical phase of secretory regulation. Analysis of enzyme dynamics (amylase, lipase and protease) showed a slight increase in the activity of proteases in pancreatic juice (by 1.2- 12.4%), compared to the control group, in the phase of complex-reflex regulation of pancreatic secretory activity related to the recognition of the taste qualities of the feed. Feed conversion ratio in the test group of broiler chickens increased by 1.52% when using acidifiers in the diet. The result of the study showed that the use of acidifiers has a beneficial effect on chickens’ gastrointestinal digestion as well as an inhibitive action on intestinal pathogens.