Rat Pancreas Tissue Research Articles

Change of α - tocopherol, cholesterol, retinol and some fatty acids in pancreatic tissue of uncontrolled diabetic rats supported by resveratrol, lipoic acid and vitamin C

In this study, the effects of resveratrol, lipoic acid and vitamin C on some biochemical changes in the pancreas tissue of rats treated with streptozotocin (STZ) type 1 diabetes were investigated. Resveratrol (R), Lipoic acid (LP), and Vitamin C (VC) were administered intraperitoneally to diabetic -1 rats without insulin. According to our findings, it was observed that the α-Tocopherol amount decreased in diabetes and diabetes + antioxidant groups when compared with the control group (p <0.01). Cholesterol levels did not differ between the groups. Palmitic acid (16: 0), palmitoleic acid (16: 1 n-7) and oleic acid (18: 1 n-9) in fatty acid composition decreased in diabetes and diabetes + antioxidant groups compared to control group. (p <0.05, p <0.01). Stearic acid (18: 0) was higher in diabetic groups than the control group. Linoleic acid (18: 2 n-6), Gamma-linolenic acid (18: 3 n-6), Arachidonic acid (20: 4 n-6) and docosahexaenoic acid (22: 6 n-3) were higher in diabetic groups (p <0.05, p <0.01). According to the results obtained, the antioxidant substances applied without insulin were insufficient in the tissues of rats with Type-1 diabetes requiring absolute insulin treatment. Also, the damaging effect of stz on the pancreas is observed when vitamin E levels decrease. Because vitamin E is an antioxidant substance that breaks down especially in the cell membrane and chain reaction in free radical reactions. The decrease in vitamin E levels can be explained by the increase in free radical levels in the tissue.

Spleen and Pancreatic Tissue Change after Genetically Modified Soybean Oil Consumption Among Male Wistar Rats

<strong data-sider-select-id="b8bf10e1-996e-4fe0-a16e-f65aaaaf6a6c">Background: Over the past 20 years, the increased consumption of transgenic products for humans and animals led to the conduction of nutritional studies in this regard. However, these studies were limited, and they did not find a definitive answer to the possible health hazards of transgenic products. Therefore, this study was designed to evaluate the effects of a diet containing transgenic soybean oil on rats. <strong data-sider-select-id="0d24514e-9731-4303-8701-71ae0f71f52c">Methods: Accordingly, male Wistar rats (N=6/group) were given a nutritionally moderate purified diet with 10% genetically modified soybean oil for 90 days. Two control groups receiving nongenetically modified soybean oil and a standardized diet were also enrolled. One-way analysis of variance (ANOVA) followed by Tuky post hoc analysis was used to compare the values between groups and to detect the effects of transgenic soybean oil. <strong data-sider-select-id="6e6a93c9-227f-45c1-a050-9f3b96ecc42d">Results: Rats fed on transgenic soybean oil demonstrated several histologic changes in pancreas tissues, including changes in severe congestion, the presence of inflammatory cells, and changes in the Langerhans islands. However, no changes were observed in the spleen, except for negligible congestion in all treatment groups. Regarding blood indicators, hemoglobin levels in the transgenic soybean oil group decreased compared to the other two groups (P<0.05). <strong data-sider-select-id="103c4ab9-7a19-4a38-8077-e5b11eb6caee">Conclusion: According to our results, a 90-day treatment with transgenic soy-based oil caused significant organ changes in the pancreas tissue of rats. Further studies evaluating the long-term effects are also needed to better elucidate these effects.

Determination of Ghrelin Immunoreactivity in the Pancreas Tissue of Rats Supplied with Melatonin

Determination of Ghrelin Immunoreactivity in the Pancreas Tissue of Rats Supplied with Melatonin

Comparison of Exosome -miRNA -9 and -146 Profiles Isolated from Brain and Pancreas Tissue in Healthy and Diabetes Rats

Small vesicles called exosomes have been found to regulate gene expression in tissues and play a role in the pathogenesis of many diseases. Therefore, this study aimed to determine the effects of exosomes on diabetes and related microRNA (miRNA) exchange. For this purpose, nicotinamide (120 mg/kg) was administered intraperitoneally (IP.), and Streptozotocin (50 mg/kg) ip was allocated 15 minutes later. Rats with 126 mg/dL and up glucose levels were accepted as Type 2 diabetes. At the end of 21 days, exosomes were obtained from the pancreas and brain tissues of rats (n:10) of diabetics and healthy groups. Then biochemical analyzes and oxidative stress parameters of both groups were examined. In addition, miRNA changes were discussed, and the results obtained were statistically evaluated. A significant increase was found in total oxidative status (TOS) and Lactate dehydrogenase (LDH) levels in both brain and pancreatic exosomes. It was observed that the total antioxidant level (TAC) decreased compared to the control group (P

Study of some physiological and histological effects of gemfibrozil Drug in Pancreas Tissue of male Albino rats

Study of some physiological and histological effects of gemfibrozil Drug in Pancreas Tissue of male Albino rats

RNA-sequencing approach for exploring the protective mechanisms of dexmedetomidine on pancreatic injury in severe acute pancreatitis.

Background: Severe acute pancreatitis (SAP) is a severe form of acute pancreatitis with the potential to cause life-threatening complications. Patients with acute SAP require surgical intervention and are admitted to the intensive care unit for non-invasive ventilation. Dexmedetomidine (Dex) is currently used by intensive care clinicians and anaesthesiologists as an adjunctive sedative. Therefore, the clinical availability of Dex makes it easier to implement in SAP treatment than developing new drugs. Methods: Randomly dividing thirty rats into sham-operated (Sham), SAP, and Dex groups. The severity of pancreatic tissue injury in each rat was assessed by Hematoxylin and eosin (HE) staining. Serum amylase activity and inflammatory factor levels were measured using commercially available kits. The expressions of necroptosis-related proteins, myeloperoxidase (MPO), CD68, and 4-hydroxy-trans-2-nonenal (HNE) were detected using immunohistochemistry (IHC). Transferase-mediated dUTP nick-end labeling (TUNEL) staining was utilized to identify pancreatic acinar cell apoptosis. The subcellular organelle structure of pancreatic acinar cells was observed using transmission electron microscopy. The regulatory effect of Dex on the gene expression profile of SAP rat pancreas tissue was investigated using RNA sequencing. We screened for differentially expressed genes (DEGs). Quantitative real-time PCR (qRT-PCR) measured critical DEG mRNA expression in rat pancreatic tissues. Results: Dex attenuated SAP-induced pancreatic injury, infiltration of neutrophils and macrophages, and oxidative stress. Dex inhibited the expression of necroptosis-associated proteins RIPK1, RIPK3, and MLKL and alleviated apoptosis in acinar cells. Dex also mitigated the structural damage caused by SAP to mitochondria and endoplasmic reticulum. Dex inhibited SAP-induced 473 DEGs, as determined by RNA sequencing. Dex may regulate SAP-induced inflammatory response and tissue damage by inhibiting the toll-like receptor/nuclear factor κB (TLR/NF-κB) signaling pathway and neutrophil extracellular trap formation. Conclusion: This study elucidated the remarkable effect of Dex against SAP and investigated the potential mechanism of action, providing an experimental base for the future clinical application of Dex in the treatment of SAP.

Bai Hu Ren Shen Soup promotes autophagy in the pancreas tissue of diabetic rats by increasing the level of autophagy protein LC3 and decreasing the level of autophagy protein p62

Bai Hu Ren Shen Soup promotes autophagy in the pancreas tissue of diabetic rats by increasing the level of autophagy protein LC3 and decreasing the level of autophagy protein p62

Ameliorative Effects of Bifidobacterium animalis subsp. lactis J-12 on Hyperglycemia in Pregnancy and Pregnancy Outcomes in a High-Fat-Diet/Streptozotocin-Induced Rat Model.

Bifidobacterium, a common probiotic, is widely used in the food industry. Hyperglycemia in pregnancy has become a common disease that impairs the health of the mother and can lead to adverse pregnancy outcomes, such as preeclampsia, macrosomia, fetal hyperinsulinemia, and perinatal death. Currently, Bifidobacterium has been shown to have the potential to mitigate glycolipid derangements. Therefore, the use of Bifidobacterium-based probiotics to interfere with hyperglycemia in pregnancy may be a promising therapeutic option. We aimed to determine the potential effects of Bifidobacterium animalis subsp. lactis J-12 (J-12) in high-fat diet (HFD)/streptozotocin (STZ)-induced rats with hyperglycemia in pregnancy (HIP) and respective fetuses. We observed that J-12 or insulin alone failed to significantly improve the fasting blood glucose (FBG) level and oral glucose tolerance; however, combining J-12 and insulin significantly reduced the FBG level during late pregnancy. Moreover, J-12 significantly decreased triglycerides and total cholesterol, relieved insulin and leptin resistance, activated adiponectin, and restored the morphology of the maternal pancreas and hepatic tissue of HIP-induced rats. Notably, J-12 ingestion ameliorated fetal physiological parameters and skeletal abnormalities. HIP-induced cardiac, renal, and hepatic damage in fetuses was significantly alleviated in the J-12-alone intake group, and it downregulated hippocampal mRNA expression of insulin receptor (InsR) and insulin-like growth factor-1 receptor (IGF-1R) and upregulated AKT mRNA on postnatal day 0, indicating that J-12 improved fetal neurological health. Furthermore, placental tissue damage in rats with HIP appeared to be in remission in the J-12 group. Upon exploring specific placental microbiota, we observed that J-12 affected the abundance of nine genera, positively correlating with FBG and leptin in rats and hippocampal mRNA levels of InsR and IGF-1R mRNA in the fetus, while negatively correlating with adiponectin in rats and hippocampal levels of AKT in the fetus. These results suggest that J-12 may affect the development of the fetal central nervous system by mediating placental microbiota via the regulation of maternal-related indicators. J-12 is a promising strategy for improving HIP and pregnancy outcomes.

Geraniol ameliorated serum lipid profile and improved antioxidant defense system in pancreas, liver and heart tissues of alloxan-induced diabetic rats

Geraniol ameliorated serum lipid profile and improved antioxidant defense system in pancreas, liver and heart tissues of alloxan-induced diabetic rats

Antihyperglycemic, Endothelial protection and Toxicity study of Basil Leaves Extract on Diabetic Rats

BACKGROUND: Diabetes Mellitus (DM) remains a serious debilitating global health problem in low- and middle-income countries with rising incidence of DM-related complications due to ineffective Diabetic control. Herbs of the Ocimum family, especially Ocimum basilicum or basil leaves, have been investigated for their antihyperglycemic properties. AIM: This study aimed to demonstrate the antihyperglycemic effect, endothelial protection, and toxicity of basil leaves on Diabetes-induced Wistar rats in vivo. METHODS: Streptozosin injections were used to induced diabetes in male Wistar rats. Basil leaves extracts 100, 300, and 1000 mg/kg BW were introduced to diabetic rats. Blood glucose levels (BGL), soluble Advanced Glycation End, tumor necrosis factor-α, interleukin (IL)-6, IL-2 were measured using enzyme-linked immunosorbent assay. Kidney and liver functions together with the histopathology reports were reported for acute, subacute, and chronic toxicity studies. RESULTS: Basil leaves exposure significantly lowers BGL (p < 0.00), but yielded no statistically significant difference between extract doses. Hemostatic parametersshowed significantly reduced endothelial dysfunction markers for all doses compared to control. Toxicity study yielded no differences between control and any doses of basil leaves in all acute, subacute, and chronic toxicity studies. Histopathological findings exhibited no evidence of tissue damage on the liver, kidney, heart, pancreas, lung, and lymph tissues in either control or experiment rats. CONCLUSIONS: Basil leaves exposure were positively associated with lower glucose level, lower endothelial activation markers on Diabetic rats. The toxicity and histopathological results of the extract are on par with control.

Hexagonal boron nitride nanoparticles trigger oxidative stress by modulating thiol/disulfide homeostasis.

Hexagonal boron nitride nanoparticles (hBN NPs) are encouraging nanomaterials with unique chemical properties in medicine and biomedical fields. Until now, the optimal hBN NP's dosage and biochemical mechanism that can be used for in vivo systems has not been fully revealed. The main aim of this article is to reveal characteristics, serum and tissue interactions and any acute cytotoxic effect of different dose of hBN NPs for the first time. hBN NPs at concentrations varying between 50-3200 µg/kg was administered by intravenous injection to Wistar albino rats (n = 80) divided into seven dosage and control groups. Blood and tissue samples were taken after 24 hours. Our findings suggested that higher doses hBN NPs caused oxidative stress on the serum of rats dose-dependently. However, hBN NPs did not affect thiol/disulfide homeostasis on kidney, liver, spleen, pancreas and heart tissue of rats. Furthermore, hBN NPs increased serum disulfide formation by disrupting the thiol/disulfide balance in rats. Also, LOOH and MPO levels increased at high doses, while CAT levels decreased statistically. The results revealed that hBN NPs induce oxidative stress in a dose-dependent manner by modulating thiol/disulfide homeostasis in rats at higher concentrations.

The Effect of High Intensity Intermittent Training (HIIT) on GLP-1R Expression in Pancreas Tissue and Serum Insulin of Male Wistar Rats with Type 2 Diabetes Mellitus

Objective: Insulin secretion and insulin resistance are associated with incidence and severity of type 2 diabetes mellitus (T2DM). This study aimed to assess the effect of high intensity intermittent training (HIIT) on GLP-1R expression in pancreas tissue and serum insulin of T2DM rats. Materials and Methods: In this quasi-experimental study, T2DM induced by intraperitoneal injection of streptozotocin-Nicotinamide in 16 male wistar rats aged 10 weeks (220 – 20 g). The rats were divided into control (no-training, n= 8) and HIIT (5 times/ week/12-week, n= 8) groups. GLP-1R expression in pancreas tissue, fasting glucose and serum insulin were measured 48 h after lasted exercise of exercise and control groups. Independent and paired T-test was used to compare variables between 2 groups. Results: HIIT resulted in significant increase in GLP-1R expression compared to control subjects (P-value: 0.023). Fasting glucose decreased (P-value< 0.0001) and serum insulin increased (P-value< 0.0001) in response to HIIT when compared to control subjects. Conclusion: HIIT can improve insulin secretion in male rats with T2DM and this improvement can be attributed to increased GLP-1R expression in pancreas tissue in response to training protocol.

Upregulated IRF9 promotes cell apoptosis of hyperlipidemia acute pancreatitis with heart injury by regulating SIRT1

Hyperlipidemia acute pancreatitis (HLAP) is a significant cause of AP, characterized by recurrent attacks, more complications and high incidence and mortality. HLAP is often accompanied by single or multiple organ damage. Negative regulation of interferon-regulatory factor 9 (IRF9) on sirtuin-1 (SIRT1) contributes to a range of diseases. However, the function of IRF9 and SIRT1, and the relationship of the two in HLAP with heart injury remain to be illustrated so far. Animal models of HLAP were set up by feeding with high-fat chow and subsequently injecting 20% L-arginine intraperitoneally. The degree of pancreas and heart tissue injury was evaluated. Heart cell apoptosis was assayed by the TUNEL technique. IRF9, SIRT1, p53 and acetylated p53 (Ac-p53) expression levels were measured by qRT-PCR and western blot. The correlation between SIRT1 and IRF9 expression levels was analyzed. Results showed that the damage degree in rat pancreas and heart tissues of AP and HLAP group was more distinctly and heart cell apoptosis was elevated. Pancreas, heart injury and cell apoptosis of the HLAP group were more remarkable than that of the AP group. Apparent increases of IRF9 and Ac-p53/p53 expression and a marked drop of SIRT1 expression were observed in the AP and HLAP group rather than NC and HLNC group. IRF9 and Ac-p53/p53 expression levels of the HLAP group were markedly raised compared with the AP group. SIRT1 expression of the HLAP group was obviously lower than that of the AP group. There was an inverse correlation between the decrease of SIRT1 and the increase of IRF9 in AP and HLAP groups. Based on the above findings, we drew a conclusion that in pancreatitis with heart injury, upregulated IRF9 inhibited SIRT1 expression, elevated the acetylation of p53, and increased myocardial cell apoptosis. Hyperlipidemia further exacerbated pancreas and heart injury and accelerated myocardial cell apoptosis. These results would furnish an experimental and theoretical basis to diagnose and therapy diseases.

Preliminary Study Results of Native Mongolian Inonotus Obliquus Extracts in Alloxan-Induced Diabetic Rats

Objectives: We aimed to investigate the morphological changes and clinical chemistry panel of alloxan-induced diabetic rats for the treatment groups of ethanol or aqueous extracts of Mongolian natural Inonotus obliquus. Methods: Wistar albino rats (n = 80) were randomly assigned into four groups: 1. Healthy control group, 2. Untreated diabetic group, 3. Diabetic group treated with Inonotus obliquus (500 mg/kg per day) water extract, 4. Diabetic group with Inonotus obliquus (500 mg/kg per day) ethanol extract. Diabetes was induced by alloxan 150 mg/kg (single intraperitoneal injection) in the diabetic groups. Results: Both water and ethanol extract of Inonotus obliquus reduced the blood glucose level of diabetes at 48 h after treatment. Histologic and morphology examinations showed that water extracts of Inonotus obliquus alleviated the damage to pancreas tissues in alloxan diabetic rats. Conclusions: We postulate that simple ethanol and water extracts of natural Mongolian Inonotus obliquus have an antihyperglycemic effect that enhances islet cell function in alloxan-induced diabetic rats. The extracts of Mongolian natural Inonotus obliquus are easily producible at low cost and should be further explored as a treatment for diabetes.

Curculigo pilosa mitigates against oxidative stress and structural derangements in pancreas and kidney of streptozotocin-induced diabetic rats.

Background Curculigo pilosa (African crocus) is widely used in folklore medicine to treat diabetes mellitus and its associated complications. This study was carried out to evaluate this traditional claim by mechanistic investigation of the effect of corn steep liquor extract of Curculigo pilosa and its n-butanol and methanol solvent fractions on hyperglycemia mediated oxidative damage in pancreas and kidney of streptozotocin-induced diabetic rats. Methods Diabetes mellitus was induced by single intraperitoneal administration of (50 mg/kg) streptozotocin and diabetic rats were treated orally with the extract(s) once in a day for 28 days. After experimental period, the effect of the extract(s) on hyperglycemia mediated oxidative stress was assessed by determination of lipid peroxidation (LPO), reduced glutathione (GSH), nitric oxide (NO), hydrogen peroxide (H2O2) and activities of catalase (CAT) and superoxide dismutase (SOD) enzymes. Also histopathology studies were conducted to substantiate the protective effects on pancreas and kidney. Results Oral administration of the extract significantly (p<0.05) mitigated the hyperglycemia mediated oxidative damage via improving the antioxidant system, inhibit the generation of lipid peroxide, hydrogen peroxide and nitric oxide. Also administration of extracts improved the structural architecture of the pancreas and kidney tissues in diabetic rats. Conclusion The results obtained in this study provide resounding scientific support for the folkloric use of Curculigo pilosa in the management of diabetes mellitus and its complications.

Launaea acanthodes (Boiss) O. Kuntze mediates hepatic glucose metabolism and ameliorates impaired pancreatic function in streptozotocin-induced diabetic rats

Ethnopharmacological relevanceLaunaea acanthodes (Boiss.) O. Kuntze is native to semiarid regions of central Iran, traditionally used in the treatment of numerous disorders including diabetes. Aim of the studyThe current study aimed to explore hypoglycemic activity of Launaea acanthodes extract in streptozotocin-induced diabetic rats. Furthermore, gene expression study was carried out to examine expression levels of key glucose metabolism-related genes. MethodsFor in vitro study, Folin-Ciocalteus, DPPH and aluminum chloride colorimetric assays were used to determine the total phenolic content, antioxidant capacity and total flavonoid content of extracts, respectively. For in vivo study, streptozotocin-induced diabetic Wistar rats were orally administered with metformin (50 mg/kg) and various doses of extracts (100, 200 and 400 mg/kg body weight) for 28 days. Fasting blood glucose, body weight, food and water intake were assessed during the course of treatment. At the end of the intervention, oral glucose tolerance test (OGTT), lipid profile and glycated hemoglobin (HbA1c) were evaluated. Furthermore, functional liver enzymes, oxidative stress markers and histopathology of pancreas were examined. Lastly, quantitative real time polymerase chain reaction (qRT-PCR) was applied to explore the mRNA levels of genes relevant to glucose metabolism in the pancreas and liver tissues of diabetic rats. ResultsBased on the in vitro results, the hydroalcoholic extract revealed potential radical scavenging activity and contained highest amount of phenolic and flavonoid. The in vivo results demonstrated that the extract lowered fasting blood glucose level, increased the body weight, restored the alterations in the levels of water and food intake, attenuated HbA1c, improved lipid profile and ameliorated the OGTT in diabetic rats. The extract administration alleviated the histopathological changes in the pancreas, suppressed malondialdehyde (MDA) level and further restored attenuated levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in diabetic rats. Analysis of real time PCR data showed that extract administration reversed the expression levels of hepatic glucokinase (GK), phosphenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase). Meanwhile, the extract upregulated the expression level of glucose transporter-2 (GLUT-2) and pancreatic-duodenal homeobox (PDX-1) in diabetic rats. ConclusionCollectively, the results demonstrate that Launaea acanthodes hydroalcoholic extract exerts hypoglycemic effect possibly via regulating key enzymes of glucose metabolism and ameliorating pancreatic dysfunction through its antioxidant properties.

Soybean Milk and Fermented Soybean Milk Administration Effect on Oxidative Damage Level in Pancreas Tissue of Hyperlipidemic Rats

Soybean Milk and Fermented Soybean Milk Administration Effect on Oxidative Damage Level in Pancreas Tissue of Hyperlipidemic Rats

Increased malondialdehyde (MDA) level in pancreas tissue of hyperlipidemic induced rats (Rattus norvegicus) by quail egg yolk (Coturnix coturnix)

Increased malondialdehyde (MDA) level in pancreas tissue of hyperlipidemic induced rats (Rattus norvegicus) by quail egg yolk (Coturnix coturnix)

Modulation of the antioxidant defense system in liver, kidney, and pancreas tissues of alloxan-induced diabetic rats by camphor.

Excess production of reactive oxygen species and the loss of antioxidant efficiency systems play an important role in the development of diabetes. Thus, using antioxidant compounds is an important strategy to reduce these complications. This study aimed to investigate the possible effect of camphor on the serum biochemical parameters and oxidative parameters in the pancreas, liver, and kidney tissues of alloxan-induced diabetic rats. In this study, 40 male Wistar rats were divided into five groups, including normal group, diabetic group, the diabetic group treated with glibenclamide, the diabetic group treated with 30mg/kg camphor, and the diabetic group receiving the camphor solvent. Intragastric administration of camphor and glibenclamide, as the control drug, to diabetic rats for 21days lowered their blood glucose, total cholesterol, triglyceride, and LDL-cholesterol, while the blood high-density lipoprotein -cholesterol level was increased. In addition, our results indicated that treatment of diabetic rats with camphor increased the activity of glutathione peroxidase, catalase, and superoxide dismutase enzymes as well as reduced glutathione content in the liver, pancreas, and kidney tissues as compared to the diabetic rats. Based on our data, it can be concluded that camphor has a hypoglycemic activity, and this effect may be attributed, in part, to its antioxidant ability. PRACTICAL APPLICATIONS: Camphor is a terpenoid natural compound derived from the wood present in the camphor laurel's stem and roots (Cinnamomum camphora L.) trees. The synthetic form of camphor is currently being produced for medical, health, and industrial applications. In addition, this compound is present in Rosmarinus officinalis, Cinnamomum zeylanicum, Salvia officinalis, Artemisia Annua, and Ocimum basilicum. Numerous researches have shown its beneficial effects on various diseases. In this study, it has been shown that camphor possesses antidiabetic effects in alloxan-induced diabetic rats. Treatment of diabetic rats with camphor increased the antioxidant capacity and reduces the oxidative stress markers in the liver, pancreas, and kidney tissues as compared to the diabetic rats. Given the favorable effect of camphor on some oxidative parameters in diabetic rats in our study, its antihyperglycemic property is probably due to its antioxidant effects.

Effect of Curculigo pilosa supplemented diet on blood sugar, lipid metabolism, hepatic oxidative stress and carbohydrate metabolism enzymes in streptozotocin-induced diabetic rats.

Diabetes mellitus (DM) has continued to raise concern globally and Curculigo pilosa (CP) is used for its treatment and management in folkloric medicine. In this study, the in vitro antioxidant abilities of CP and the effects of CP-supplemented diets on blood sugar, lipid metabolism, oxidative stress and key carbohydrate metabolizing enzymes in streptozotocin (STZ)-induced diabetic rats were investigated. Polyphenol contents (total phenolic and total flavonoid) and antioxidant ability of different extracts of CP were determined in vitro. Diabetes mellitus were stimulated in healthy rats by single intraperitoneal administration of 50mg/kg streptozotocin and it was confirmed by elevated blood glucose level after 3days. Thirty six rats were distributed into six groups of six rats each and diabetic rats were fed with 5 and 10% CP-supplemented diet for 21days. Thereafter, the effects of the dietary regimen were evaluated on blood glucose, body weight, hepatic carbohydrate metabolizing enzymes, lipid profile, oxidative stress markers, serum markers of hepatic and renal damages and histopathology studies. Different extracts of CP contained polyphenol contents and exhibited antioxidant properties in different models used. Diabetic rats showed elevated level of blood glucose and body weight loss. Treatment of diabetic rats with CP-supplemented diet significantly (p < 0.05) lowered the blood glucose and improved body weight loss. Also, the treatment with the CP-supplemented diet significantly (p < 0.05) enhanced the activities of hepatic glycolytic (hexokinase and glucose-6-dehydrogenase) and lowered the gluconeogenic (fructose 1, 6 biphosphatase and glucose-6-phosphatase) enzymes in diabetic rats. The lipid profile, oxidative stress markers and serum markers of hepatic and renal damages were significantly (p < 0.05) restored to near normalcy in the diabetic rats. Histopathological slides also showed improvements in pancreas and hepatic tissues of diabetic rats treated with CP-supplemented diet. Data obtained in this study suggested that CP-supplemented diet could be used as dietary regimen in the management of DM.