Objectives: Pancreas enzyme production can be activated by stimulation of the gland with specific gastrointestinal hormones. This affects the metabolism and thereby the blood flow. We evaluated whether the administration of the gastrointestinal hormones Secretine and Cholecystokinine combined with ultrasound contrast enhancement could be useful for evaluating pancreatic tumors. Methods: We studied 14 pigs and 10 patients with ultrasound percutaneously or intraoperatively. Percutaneously we did a B-mode examination first, then a contrast enhanced examination of pancreas and after 5 min and followed by another examination of pancreas using both Secretine, Cholecystokinine and SonoVue. Intraoperatively the scanning procedure of pancreas was repeated. The sonographic images were stored and afterwards evaluated. (Agents used: 2, 4 ml SonoVue.1U/kg Secretine and 0, 04 mcg/kg cholecystokinin. Ultrasonographic equipment: 3.5/7 MHz curved/linear transducer; GE). Results: Use of contrast-enhanced ultrasonography increases the echo-pattern of pancreas. The administration of secretine and cholecystokinine in combination with SonoVue increases the echo-pattern of pancreas even more explicitly. Conclusions: Since adenocarcinomas in pancreas are composed of excretory inactive tissue, it makes it simpler to discriminate between normal pancreatic tissue and adenocarcinomas by enhancing the contrast between the different tissues with the specific gastrointestinal hormones and contrast agent.