Palmitic Content Research Articles

Magnesium Stearate Fatty Acid Composition, Lubrication Performance and Tablet Properties

Magnesium stearate (MgSt) is a common tablet lubricant. As variations in MgSt properties are known to influence tablet attributes, the impact of MgSt fatty acid composition, particularly the significance of the stearate and palmitate contents, and its effects on tablet properties warrant further investigation. This study investigated the effect of MgSt with different stearate and palmitate contents but comparable physical properties (e.g. particle size, crystallinity, specific surface area and morphology) on lubrication performance and resulting tablet quality attributes, including mechanical strength, disintegratability and drug release. The influence of MgSt concentration and blending duration on the resulting tablet properties was also examined. Tablets produced using the lower stearate content MgSt had slightly higher tensile strength. The effect of MgSt stearate content was more apparent in the disintegration time and drug release, whereby MgSt of lower stearate content resulted in tablets with longer disintegration time and slower drug release. The lower stearate content also resulted in a lower lubrication performance, leading to a lesser reduction in tablet ejection force. As expected, a longer blending time of the tablet formulation blend with MgSt yielded tablets with reduced tensile strength, shorter disintegration time and slower drug release. Tablets with higher MgSt concentration showed a greater reduction in tensile strength, longer disintegration time and faster drug release. The study findings reinforced observations by other researchers and provided a better understanding of the fatty acid composition effects of MgSt on lubrication performance and the resulting tablet properties.Graphical

Deciphering the behind blackspot exocarp disorder in avocado cv. Hass through a hormonal, transcriptional and metabolic integration approach

Avocado cv. Hass is an important sub-tropical crop with an increasing global demand. However, the avocado supply chain experiences significant fruit losses, particularly during the postharvest stage due to diseases and disorders that manifest after prolonged cold storage or the ready-to-eat stage. The blackspot exocarp disorder, which appears as brown or black blotches only after extended cold storage conditions, leads to substantial commercial losses for exported avocados. This research aimed to identify transcriptomic, metabolomic, and hormonal changes in avocado fruits affected by blackspot disorder, differentiating between the green and black exocarp tissues directly impacted by this physiological disorder. The results showed a correlation between the black-colored exocarp of blackspot affected fruits with high levels of gibberellins, cytokinins, jasmonic acid and salicylic acid hormones. Metabolically, these changes were accompanied by a high fatty acid content of oleate, palmitate and linoleate. Using a metabolic pathway reconstruction analysis, we integrated hormonal and metabolic data with transcriptomic information. This approach identified several genes involved in central carbon metabolism, long-chain fatty acid elongation, and jasmonate/salicylate biosynthesis pathways, as well as a possible accumulation of lignins due to a high expression of genes associated with the phenylpropanoid pathway in the black exocarp of blackspot-affected fruits. These findings suggest that blackspot disorder results from a combination of plant defense mechanisms triggered to strengthen the fruit exocarp tissue.

Genetic analysis of the content of palmytic acid glycerides in the carriers of maize mutation shrunken-1

Aim. The influence of corn shrunken-1 (sh1) mutation on the content of palmitate in corn oil establishing and genetic analysis of this trait. Methods. The effects of the sh1 mutation on the content of palmitate were determined by comparing the inbreds – carriers of this mutation with inbreds of the common type, as well as in the top-crosses of common type inbreds with the mutant inbreds. The genetic components of the variance in terms of the content of palmitate were analyzed in the diallel crosses of mutant inbreds according to the second Griffing method. The determination of oil fatty acid composition was carried out by the Peisker gas chromatographic method. Results. It has been established that the corn inbreds – carriers of sh1 mutation exceed the common type inbreds by an average of 29.2 % in terms of palmitate content. The level of the trait in the inbreds based on the sh1 mutation was notable as having the quantitative variability and varied within 12.2-15.6 %. The content of palmitate in the carriers of sh1 mutation was inherited as incomplete dominance with a predominant contribution of additive effects to the variance. A higher level of the trait was controlled by recessive alleles of polygenes. Conclusions. The obtained results suggests the possibility of spatial linkage of the sh1 mutant gene with palmitate-coding locus, the effect of which is modified by the polygenic complex. It has been confirmed, that the carriers of sh1 mutation expands the genetic diversity of corn in terms of palmitate content.

Effect of solvent extraction on the key aroma components of Tamarindus indica L. pulp

The volatile organic components (VOCs) of Tamarindus indica L. (TIL) pulp and the extraction of TIL were analyzed by headspace solid-phase microextraction with gas chromatographymass spectrometry (HS-SPME–GCMS) to characterize key odorants and investigate their changes during solvent extraction (SE). A total of 109 VOCs were identified in TIL pulp. Twenty-two odorants were determined to be key odorants, and 12 differential aroma components were identified using the relative odor activity value (ROAV) method and multivariate statistical analysis. Among these, phenylacetaldehyde, nonanal and phenethyl alcohol were the common key aroma components of four TIL pulps. In addition, the effect of SE on the aroma composition of TIL was analyzed according to changes in the glucose and fructose contents of TIL pulp combined with sensory evaluation. The results showed that SE affected the aroma of TIL pulp, among which the contents of ethyl palmitate, diethyl succinate, 1-tetradecene, 1-hexadecene, heptadecane, ethyl phenylacetate, and ethyl oleate showed significant differences before and after extraction. The present research refines the existing aroma composition system of TIL and provides a reference for further development and utilization of TIL.

Analysis of the contribution of koji-making with Z. rouxii on volatile compounds of soy sauce

The co-inoculation fermentation of different microorganisms plays a crucial role in soy sauce fermentation in terms of enriching the composition of the volatile compounds. Therefore, the present study sought to investigate the effect of co-inoculation fermentation of Aspergillus oryzae and Zygosaccharomyces rouxii on the dynamics of volatile compounds in soy sauce using the combination of gas chromatography-mass spectrometry (GC-MS) and multivariate correlation analysis. A total of 124 volatile compounds were identified by A. oryzae and Z. rouxii inoculation alone and co-inoculation, including hydrocarbons, fatty acids, phenols, fatty esters, and benzamides. The content of phenylethyl alcohol, ethyl linoleate, and ethyl palmitate was significantly increased, and the metabolic pathways of multiple volatile compounds were affected in soy sauce inoculated with Z. rouxii. The present study results potentially contribute to the optimization of soy sauce fermentation processes.

TEST OF BIODIESEL FROM USED COOKING OIL FROM DORMITORY X ON OPACITY AND EXHAUST EMISSIONS HC, CO AND CO2

The current trend is the use of biodiesel as an alternative fuel, one alternative is used cooking oil. Not many Indonesian people are aware of the great potential for utilizing used cooking oil or what is commonly called cooking oil (waste vegetable oil or WVO, also known as waste cooking oil or WCO and used cooking oil or UCO). The aim is to minimize pollution of used/used cooking oil waste in the surrounding environment as environmental conservation, to know the characteristics and quality requirements of used cooking oil/used cooking oil from dormitory X to become biodiesel according to SNI 04-7182-2015 standards, as well as the effect of blending biodiesel from cooking oil used/used cooking oil from dormitory X with PT Pertamina's Solar TBBM on opacity, performance and exhaust emissions of diesel engines. Test results for used cooking oil test criteria for smell, taste, visual color, water content, free fatty acids, acid number and peroxide value do not meet the quality standards of SNI 01-3741-2002, so they are not suitable for use or consumption again because they are toxic. The highest yield value is 81.33% and the lowest is 61.7%. Analysis of the FAME content was 98.18% and the remaining 0.72% glycerol. The highest content of methyl palmitate was 47.57% and the lowest content was 1.502% methyl myristate. Density test results were 815 – 880, viscosity were 2.3 – 6.0 cST, water content were 210,667 – 366,750, ASTM Copper Strip color test and copper blade corrosion were sequentially number 3 and class 1 A. The highest opacity value was achieved at 3000 rpm rotation . Emissions of hydrocarbons (HC) produce no HC on average. Carbon monoxide (CO) exhaust emissions produce an average of 0.05% by volume. Emission levels of CO2 in exhaust gas show an average of 0.2% by volume.

Genetic analysis of palmytic acid content in the oil of maize based on Waxy mutation

Aim. Determination of the effect of wx mutation on the palmitate content and genetic analysis of this trait in waxy maize. Methods. In two-year experiments, 10 unrelated maize inbreds of the common type and 10 inbreds – carriers of the wx mutation were analyzed. Genetic analysis of palmitate content was carried out in the diallel crosses of 6 indreds – carriers of the wx mutation by the second Griffing method. The palmitate content was determined by Peysker’s gas chromatographic method. Results. Carriers of the wx mutation of maize exceeded the maize of common type in the content of glycerides of palmitic acid in oils. The content of palmitate in unrelated inbreds – carriers of the wx mutation was subjected to quantitative variability and ranged from 13.3 % to 16.4 %. Inheritance of palmitate content in the waxy corn was carrired out by the type of incomplete dominance with a predominant contribution of additive effects to the variation. A higher level of trait was controlled by recessive alleles of polygens. Conclusions. The obtained results suggest the presence in chromosome 9 of spatial linkage of the mutant wx gene with palmitate - coding locus, the effect of which is modified by a polygenic complex.

Effect of bovine milk fat-based infant formulae on microbiota, metabolites and stool parameters in healthy term infants in a randomized, crossover, placebo-controlled trial

BackgroundNatural enrichment of sn-2 palmitate content of infant formulae by using bovine milk fat is known to reduce formation of faecal fatty acid soaps and to improve stool consistency, but effects on gut microbiota composition are unknown. The purpose of this study was to test the influence of milk fat-based formula high in sn-2 palmitate on the infants’ gut microbiota composition and to confirm the beneficial effects of the formula on formation of faecal fatty acid soaps and stool consistency.MethodsTwenty-two healthy term, formula-fed infants were enrolled in a single-blinded randomized, crossover, placebo-controlled trial. After a 2-week run-in period, infants received either a 50% milk fat-based formula containing 39% sn-2 palmitate (MF) or a vegetable fat-based formula (VF) containing 10% sn-2 palmitate in a 2 × 2-week crossover design. Faecal microbiota composition was the primary outcome of the study. Other outcomes included faecal fatty acid soap excretion, calcium excretion, gut comfort parameters and faecal metabolites.ResultsMicrobiota analysis showed that bifidobacteria dominated the gut microbiota of most infants. Neither alpha- nor beta-diversity was significantly influenced by the intervention. Also, abundance of metabolic pathways was independent of the intervention. The MF formula resulted in significantly lower faecal levels of palmitic acid soap (p = 0.0002) and total fatty acid soaps (p = 0.0001) than the VF formula. Additionally, calcium excretion and palmitic acid concentration were significantly (p = 0.0335) lower in stool samples after MF intervention. Furthermore, a significant physiological effect on softer stools was observed in the MF intervention compared to the VF intervention (p = 0.02).Of the 870 measured faecal metabolites, 190 were significantly different after MF and VF intervention (FDR corrected p < 0.05). Most of these were found at higher levels after MF intervention, potentially indicative of the complex structure of milk fat. Metabolites with more than twofold change between interventions were mostly lipid-derived and included several milk fat-specific fatty acids.ConclusionsReplacing part of the vegetable fat in infant formula with bovine milk fat with high sn-2 palmitate levels did not change the microbiota composition, although a reduction in faecal palmitate soaps, total fatty acid soaps and calcium excretion while improving stool consistency in the MF intervention was confirmed. In addition, 190 faecal metabolites were significantly different, many related to the fat source.Trial registrationNetherlands Trial Registry Identifier: NL7815 19/06/2019.

Nutritional quality evaluation of oil and fatty acid profile in various genotypes/varieties of Indian mustard [Brassica juncea Czern &amp; Coss (L.)

Brassicas are one of the most agronomically eminent oilseeds that are employed as a variety of oilseed, vegetable, and fodder crops. The experiment was conducted with thirty-four genotypes/varieties seeds of Indian mustard [Brassica juncea Czern &amp; Coss (L.)] for oil content, Iodine value, oil stability index and fatty acid composition during 2018-2019. The experiment was laid out in completely randomized design with three replications. The range of variability of contents of oil, palmitate, stearate, oleate, linoleate, linolenate, ecosenate, doecosenate, iodine value and oil stability index varied from 33.52 to 42.15%, 1.53 to 4.98%, 0.16 to 2.71%, 5.06 to 17.78%, 17.88 to 32.15%, 11.82 to 19.85%, 5.44 to 11.89%, 28.82 to 47.66%, 114.43 to 131.71 and 1.08 to 1.99, respectively. The Brassica juncea genotype-KMR-15-6 followed by genotype- KMR-17-6 had the higher oil content, oleic acid content and low value of erucic acid which indicates that seed oil this B. juncea species genotype is possibly suitable for both human consumption and industrial purposes.

Stability and Applicability of Retinyl Palmitate Loaded Beeswax Microcapsules for Cosmetic Use

In our previous study, retinyl palmitate was successfully encapsulated by melt dispersion using waxes as shell materials. Herein, the objective of the present research is to evaluate the shelf life and kinetic release of the developed microcapsules. The study was conducted by measuring actual loading capacity over a period of time using spectroscopic analysis. The transfer percentage of particles from nonwoven facial wipes to skin-like surfaces was also investigated by simulating the rubbing mechanism with a robotic transfer replicator. Although particles stored as powder form under room temperature showed only eight days of shelf-life, particles stored as a dispersion in a refrigerator maintained 60% of the theoretical loading capacity after one month. The kinetic release profile of the particles in ethanol with shaking at 100 rpm and 37±2°C showed an initial burst in the first half an hour, followed by a sustained release. It also showed that 98% of the retinyl palmitate content released within 4 h. Particles incorporated into wet nonwoven wipes gave approximately 22% transfer to skin-like fabric. Thus, the study shows potentials of delivering skincare properties by means of retinyl palmitate capsule loaded textile substrates.

Integrated Metabolome and Transcriptome Analysis of Fruit Flavor and Carotenoids Biosynthesis Differences Between Mature-Green and Tree-Ripe of cv. "Golden Phoenix" Mangoes (Mangifera indica L.).

The commodity value of fruits is directly affected by fruit flavor and color. Secondary metabolites, such as amino acids, organic acids, esters, and β-carotene, are important synthetic products, which are of great significance in the flavor formation of mango fruits. In this study, a total of 309 different metabolites, consisting of organic acids, amino acids, phenolic acids, and saccharides, and a further 84 types of volatile organic compounds (VOCs) were identified in differential levels in TR vs. MG mango fruit stages. The major volatile compounds found were ester [2(3H)-furanone, 5-ethyldihydro; N-(2,5-ditrifluoromethylbenzoyl)-D-alanine, pentyl ester; and Octanoic acid, ethyl ester], aldehyde (benzaldehyde, 3-ethyl, and nonanal), and phenol [2-(1,1-dimethylethyl)-6-(1-methylethyl) phenol]. The analysis of carotenoid contents identified 68 carotenoids and we report for the first-time significant contents of zeaxanthin palmitate and (E/Z)-phytoene in mango fruits. α-carotene was a further major contributor to carotene contents with lesser contributions from 5,6epoxy-lutein-caprate-palmitate, β-carotene, lutein oleate, and β-cryptoxanthin. What is more, lutein content was significantly decreased in TR vs. MG fruit. RT-qPCR analysis revealed that relative to the MG stage, the expression of carotenogenic genes GGPS, PSY, LCYB, and ZEP was downregulated in TR mango fruit, whereas the transcript levels of PSD, CHYB, and NCED were downregulated. Additionally, the transcription level of some transcription factors (MYB, bHLH, and NAC) was highly correlated with pigment content in the pulp and may be responsible for carotenoid accumulation. The results describe major differences in metabolic pathways during the transition from MG to the TR stage of fruit ripening that are likely to contribute alterations in fruit flavor and provide several associated genes to be further studied in mango fruit.

Contribution of bread and biscuits to vitamin A daily requirement of preschool children in Lagos State, Nigeria

Introduction and aim. Vitamin-A-deficiency is a public health problem among preschool children of Nigeria. Study determined the contribution of bread and biscuits to vitamin A-daily-requirement of preschool children in Lagos-State, Nigeria. Material and methods. A community-based-study using a cross-sectional-design with analytical component was carried out from 2013-2015. Multi-stage-sampling-technique was used to select mothers of preschool-children (n=1599) in 5 Local-Government- Areas of Lagos. Respondents’ socio-demographic information and samples consumption-pattern were collected using validated, food-frequency-questionnaire/dietary recall. Retinyl palmitate content of randomly selected commonly-consumed brands of oven-fresh-bread stored for 5-days and biscuits (30- to 60-days) at prevailing outdoor-market-temperatures were analysed using High-Performance-Liquid-Chromatography. Contribution to preschool children’s vitamin A-daily-requirements were determined. Data were analysed using Student’s t-test and ANOVA at p&lt;0.05. Results. Mean age of preschool children was 31.44±5.28 months. Mean intakes of samples were bread (117.6 ±15.9 g/d) and biscuits (59.8±27.9 g/d). Range of contribution to vitamin-A-daily-requirement of preschool-children was 0-178.4 %. Samples contribution to vitamin A-daily-requirement of pre-school-children were oven-fresh bread (68.3 %); 5 days bread (20.7%); 30-days biscuits (25.0%) and 60-days biscuits (6.8%). Overall contribution to vitamin A-daily-requirement were bread (51.4%) and biscuits (22.4%). Statistically significant difference existed between samples contribution and vitamin-A-daily-requirement of preschool children. Conclusion. Bread and biscuits samples contributed significantly to the vitamin-A-daily-requirement of preschool children.

A Comparative Metabolomic Analysis Reveals Difference Manufacture Suitability in "Yinghong 9" and "Huangyu" Teas (Camellia sinensis).

“Yinghong 9” is a widely cultivated large-leaf variety in South China, and the black tea made from it has a high aroma and strong sweet flavor. “Huangyu” is a light-sensitive tea variety with yellow leaves. It was cultivated from the bud-mutation of “Yinghong 9” and has a very low level of chlorophyll during young shoot development. Due to chlorophyll being involved in carbon fixation and assimilation, the changes in photosynthesis might potentially affect the accumulation of flavor metabolites, as well as the quality of “Huangyu” tea. Although “Huangyu” has a golden yellow color and high amino acid content, the mechanism underlying the formation of leaf color and drinking value remains unclear. The widely targeted metabolomics and GC-MS analysis were performed to reveal the differences of key metabolites in fresh and fermented leaves between “Yinghong 9” and “Huangyu.” The results showed that tea polyphenols, total chlorophyll, and carotenoids were more abundant in “Yinghong 9.” Targeted metabolomics analysis indicated that kaempferol-3-glycoside was more abundant in “Yinghong 9,” while “Huangyu” had a higher ratio of kaempferol-3-glucoside to kaempferol-3-galactoside. Compared with “Yinghong 9” fresh leaves, the contents of zeaxanthin and zeaxanthin palmitate were significantly higher in “Huangyu.” The contents of α-farnesene, β-cyclocitral, nerolidol, and trans-geranylacetone, which were from carotenoid degradation and involved in flowery-fruity-like flavor in “Huangyu” fermented leaves, were higher than those of “Yinghong 9.” Our results indicated that “Huangyu” was suitable for manufacturing non-fermented tea because of its yellow leaf and flowery-fruity-like compounds from carotenoid degradation.

Chemical Composition, Protective Effects, and Mechanisms of Volatile Oil from Fructus Gleditsiae Abnormalis with Nasal Administration against Ischemic Injury in HFD and MCAO-Induced Rats.

Fructus Gleditsiae Abnormalis (FGA) has been used as a traditional Chinese medicine (TCM) for the treatment of stroke caused by phlegm and blood stasis. However, its substance basis and mechanism of action are currently unknown. This study is aimed to analyze the constituents of the volatile oil in FGA (VOFGA) using gas chromatography coupled with mass spectrometry (GC-MS) and explore the underlying effects and mechanisms of VOFGA in the prevention and treatment of ischemia stroke. An in vivo ischemia model was constructed by combination treatment of high-fat diet (HFD) and middle cerebral artery occlusion (MCAO) method. After administration, the cerebral infarction volume, the brain water content, hemorheology, blood lipids, inflammatory factors, oxidative stress indicators, Bax, Bcl-2, and cleaved caspase-3 and histological examination (HE) were determined and observed to explore the underlying effects and mechanisms of VOFGA against ischemia stroke. The results showed that forty components were determined after analyzed by GC-MS, and the percentage content of palmitate, paeonol, violetone, linalool, salpinol, citral, and methyleugenol were 4.69%, 5.2%, 3.56%, 3.31%, 2.42%, 2.65%, and 1.67%, respectively. The high dose of VOFGA could inhibit neurological damage; reduce the cerebral infarction volume and brain water content; improve whole blood viscosity and red blood cell aggregation index at various shear rates; reduce the levels of TG, TC, LDL-C, TNF-α, IL-1β, MDA, and NO; increase the contents of HDL-C, IL-10, and SOD; downregulate the expressions of Bax and cleaved caspase-3 in the ischemic regions; and upregulate the expressions of Bcl-2. These effects implied that VOFGA may exert neuroprotective effects via inhibiting ischemia-triggered oxidative damage-regulating blood lipid factors and reducing the production of proinflammatory mediators against cerebral I/R injury and neuronal apoptosis. The VOFGA presents a potential treatment value for cerebral ischemic stroke, and it may offer insights into discovering new active compounds for the treatment of ischemic stroke.

Metformin Weakens the Angiogenic Potential of Human Bone Marrow Mesenchymal Stem Cells (hBMSCs) by Activating Mammalian Target of Rapamycin (mTOR) Signaling

To study metformin’s effect on the angiogenesis of human bone marrow mesenchymal stem cells (hBMSCs). Cells were treated with metformin (0.5, 1, 10, 50, 100, 200 and 500 βM) for 14 days, followed by analysis of cell viability and total fatty acid profile, level of VEGFR-2, Tie-2, VE-Cadherin and mTOR signaling protein, cell differentiation by microtubule generation and cell migration by transwell assay. Metformin dose dependently decreased cell survival and reduced palmitate, oleate, stearate and linoleate content. In addition, it downregulated VEGFR-2 and Tie-2 and decreased the angiogenic potential of BMSCs and down-regulated VE-Cadherin. Western blot and PCR analysis showed that metformin activated mTOR signaling and up-regulated the transcription of autophagyrelated genes. Metformin can reduce BMSCs angiogenic potential by regulating mTOR signal pathway.

Stearoyl-CoA Desaturase (SCD) Induces Cardiac Dysfunction with Cardiac Lipid Overload and Angiotensin II AT1 Receptor Protein Up-Regulation.

Heart failure is a major cause of death worldwide with insufficient treatment options. In the search for pathomechanisms, we found up-regulation of an enzyme, stearoyl-CoA desaturase 1 (Scd1), in different experimental models of heart failure induced by advanced atherosclerosis, chronic pressure overload, and/or volume overload. Because the pathophysiological role of Scd1/SCD in heart failure is not clear, we investigated the impact of cardiac SCD upregulation through the generation of C57BL/6-Tg(MHCSCD)Sjaa mice with myocardium-specific expression of SCD. Echocardiographic examination showed that 4.9-fold-increased SCD levels triggered cardiac hypertrophy and symptoms of heart failure at an age of eight months. Tg-SCD mice had a significantly reduced left ventricular cardiac ejection fraction of 25.7 ± 2.9% compared to 54.3 ± 4.5% of non-transgenic B6 control mice. Whole-genome gene expression profiling identified up-regulated heart-failure-related genes such as resistin, adiponectin, and fatty acid synthase, and type 1 and 3 collagens. Tg-SCD mice were characterized by cardiac lipid accumulation with 1.6- and 1.7-fold-increased cardiac contents of saturated lipids, palmitate, and stearate, respectively. In contrast, unsaturated lipids were not changed. Together with saturated lipids, apoptosis-enhancing p53 protein contents were elevated. Imaging by autoradiography revealed that the heart-failure-promoting and membrane-spanning angiotensin II AT1 receptor protein of Tg-SCD hearts was significantly up-regulated. In transfected HEK cells, the expression of SCD increased the number of cell-surface angiotensin II AT1 receptor binding sites. In addition, increased AT1 receptor protein levels were detected by fluorescence spectroscopy of fluorescent protein-labeled AT1 receptor-Cerulean. Taken together, we found that SCD promotes cardiac dysfunction with overload of cardiotoxic saturated lipids and up-regulation of the heart-failure-promoting AT1 receptor protein.

Natural diversity in the carotene, tocochromanol and fatty acid composition of crude palm oil

Crude palm oil (CPO) is extracted from the mesocarp of oil palm (Elaeis guineensis) fruits. CPO is widely consumed in many African countries. Due to its high provitamin A carotenoid content, it is also widely used in programmes designed to prevent vitamin A deficiency. Elaeis guineensis occurs naturally across a wide geographical range in Africa. We investigated the carotene, tocochromanol (vitamin E) and fatty acid composition of a large set of genotypes representative of this genetic and geographic diversity. We found considerable intraspecific diversity in most lipid traits. Populations from Côte d'Ivoire were distinguished from other origins by their very low palmitate content and high tocochromanol content. Genotypes from Benin, Côte d'Ivoire and Nigeria were characterized by high carotene contents. Finally, hybrids of crosses between genotypes from Côte d'Ivoire and Nigeria produce CPO with exceptionally high provitamin A and vitamin E contents together with low palmitate content.

Association studies and QTL mapping for soybean oil content and composition

Soybean oil is one of the most important vegetable oils in world. Increasing oil content and improve its composition is an important aim of many soybean breeding programs. In this context, the objective of this study was to identify QTLs controlling oil and fatty acid contents in soybean seed in different locations. For this, we developed F2-derived populations by crossing CS303TNKCA and FA22 lines, and analyzed oil and fatty acid content in four locations in Brazil. We evaluated the correlations of traits in each location and between locations and used 1536 SNPs to molecular characterize this population. QTLs associated with the average values of four environments were identified by using simple interval mapping. A linkage map was generated using 534 SNP markers, and 25 linkage groups were formed. A total of 20 QTLs controlling oil, palmitic, stearic, oleic, linoleic and linolenic contents were found, varying from one (linolenic content) to six (palmitic content), and explaining from 7.02% (qSte-13) to 70.37% (qLin-14) of phenotypic variation. We could not associate eight QTLs (qPal-02, qOle-02, qLol-02, qOle-04, qLol-04, qPal-13.2, qSte-15 and qSte-17) with reported QTLs and genes, so these could be set as new QTLs controlling fatty acid composition. The results found in the present work can help understand the genetic basis of these traits and help breeders to modify oil content and composition in soybean seed, especially in tropical conditions.

Hierarchical Gold Mesoflowers in Enzyme Engineering: An Environmentally Friendly Strategy for the Enhanced Enzymatic Performance and Biodiesel Production.

To expand the field of nanomaterial and engineering of enzyme in eco-friendly processes, gold mesoflower (Au-MF) nanostructure was applied for preparation of three series of immobilized lipase (Au-MF/SAM 1-3) through biofunctionalization of surface by Ugi multicomponent reaction. The synthesized Au-MF/SAM 1-3/lipase as unique biocatalysts was confirmed by different analytical tools and techniques. Compared to the free lipase, the Au-MF/SAM 1-3/lipase showed more stability at high temperature and pH. Also, these biocatalysts showed high storage stability and reusability after 2 months and eight cycles, respectively. Moreover, the kinetic behavior was investigated and the results showed a minimal impairment of catalytic activity of immobilized lipase. The kinetic constants of the immobilized lipase, Au-MF/SAM 2/lipase, are Km = 0.37 mM, Vmax = 0.22 mM min-1, and kcat = 154 min-1. The immobilized lipase showed smaller activation energy (Ea) than that of free enzyme, indicating that the immobilized enzyme is less sensitive to temperature. In the following, the biodiesel production from palmitic acid was studied in the presence of Au-MF/SAM 2/lipase as an efficient biocatalyst. The influence of different reaction parameters such as temperature, molar ratio of alcohol to palmitic acid, water content, and lipase amount was deeply investigated.

Control the Mechanical Properties and Degradation of Poly(Glycerol Sebacate) by Substitution of the Hydroxyl Groups with Palmitates.

Mechanical properties and degradation profile are important parameters for the applications of biodegradable polyester such as poly(glycerol sebacate) in biomedical engineering. Here, a strategy is reported to make palmitate functionalized poly(glycerol sebacate) (PPGS) to alter the polymer hydrophobicity, crystallinity, microstructures and thermal properties. The changes of these intrinsic properties impart tunable degradation profiles and mechanical properties to the resultant elastomers depending on the palmitate contents. When the palmitates reach up to 16 mol%, the elastic modulus is tuned from initially 838 ± 55 kPa for the PGS to 333 ± 21 kPa for the PPGS under the same crosslinking conditions. The elastomer undergoes reversible elastic deformations for at least 1000 cycles within 20% strain without failure and shows enhanced elasticity. The polymer degradation is simultaneously inhibited because of the increased hydrophobicity. This strategy is different with other PGS modifications which could form a softer elastomer with less crosslinks but typically lead to a quicker degradation. Because the materials are made from endogenous molecules, they possess good cytocompatibility similar to the PGS control. Although these materials are designed specifically for small arteries, it is expected that they will be useful for other soft tissues too.