Palmitic Acid Content Research Articles

Tobacco cv. Solaris seed cake as feed for light lambs: growth performance, carcase traits and meat quality

Sixteen crossbred male lambs, housed in eight pens, were fed two isoenergetic and isonitrogenous diets: Control (CTR; n = 8) and Nicotiana tabacum cv. Solaris seed cake (SSC; n = 8). During the trial, lasted 35 days, pen dry matter (DM) concentrate consumption was calculated, individual body weight recorded, and average daily gain (ADG) calculated. Physico-chemical analyses were carried out on samples of Longissimus dorsi (LD) and Semimembranosus (SM) muscles. Animals fed Solaris seed cake showed lower (p < 0.05) ADG and slaughter body weight than CTR group. Diet did not affect the DM concentrate consumption. CTR lambs had higher cold carcase weight and yield (p < 0.01), while the proportion of commercial cuts did not differ between groups. Diet did not affect meat colour; the pH was higher (p < 0.01) for SSC group. Meat total lipid, collagen and cholesterol contents were unaffected by the diet. Fatty acid composition showed lower palmitic acid (C16:0, p < 0.05) and higher stearic acid (C18:0, p < 0.01) content in SSC compared to CTR fed lambs. The LD muscle showed lower total lipid (p < 0.01) and collagen content (p < 0.05) than SM muscle, as well as higher proportion of total SFA (p < 0.01) and lower proportion of total PUFA (p < 0.01), n-3 PUFA (p < 0.05) and n-6 PUFA (p < 0.01). Further studies are needed for a better evaluation of the potential of the innovative tobacco Solaris co-product in lambs’ diet.

Evaluation of Oxidation Stability of Organic and Conventional Hazelnut Oils

In this study, the oxidation stabilities of organic hazelnut oils (OHO) were compared to conventional hazelnut oils (CHO). For this purpose, oxidation parameters such as peroxide value (PV), free fatty acid (FFA), anisidine value (AV), volatile lipid oxidation compounds (VLOC), fatty acid composition, and total antioxidant capacity (TAC) of the hazelnut oil samples were investigated under conditions of accelerated storage. At the beginning of storage, OHO had lower PV, higher AV, TAC and linoleic acid content than CHO and similar FFA, VLOC, palmitic, stearic, and oleic acids contents to CHO. As expected, PV, FFA and AV increased in OHO and CHO during storage, while TAC decreased. No significant difference between OHO and CHO was observed in terms of FFA, TAC, palmitic and stearic acid contents at the accelerated storage condition. When compared to CHO, OHO showed lower oleic acid and higher linoleic acid at the end of storage. During storage, the highest AV and PV were determined in OHO. Results reveal that OHO has lower oxidation stability than CHO. According to these results, it can be recommended to consume the OHO without being exposed to long-term oxidation.

Quality analysis of Idesia polycarpa fruit oil samples from cultivars with different phenotypes.

To study the quality characteristics of Idesia polycarpa fruit oil (IPFO) samples under varying phenotypic traits and their differences, three typical phenotypic fruits of the same cultivar were compared, both morphologically and chemically. The results revealed that the highest water content (51.90%) occurred in small red fruits (RID-S), and the highest oil content (20.63%) was obtained in large yellow fruits (YID). A total of 532 aroma compounds were identified via gas chromatography-mass spectrometry (GC-MS), with terpenes as the major contributors. The fatty acid composition was dominated by linoleic acid (LA), palmitic acid and oleic acid, and the LA content was highest in large red fruits (RID-B) (1633.25-1807.21μg/mL), whereas the palmitic acid and oleic acid contents were highest in YID (863.72-976.70μg/mL and 505.96-604.04μg/mL, respectively). The oil quality of I. polycarpa varied according to its phenotypic characteristics, with RID-B identified as an excellent cultivar, followed by YID and RID-S.

Effect of structured lipids as dietary supplements on the fatty acid profile, carcass yield, blood chemistry, and abdominal fat deposition of female broilers

An experiment was conducted to evalute the effects of adding palm olein (POL), modified palm olein (high degree of acyl migration palm olein, H-AMD), and lard (total fatty acid saturation degree is similar to palm olein) to the diet of broilers. The study assessed production performance, fatty acid absorption, and abdominal fat deposition. A total of 100 one-week-old female broiler chicks were randomly assigned to three-tiered pens and fed five experimental diets. Enzymatic interesterification of POL causes acyl migration, transforming 1-palmitoyl-2,3-dioleoyl-sn-glycerol (sn-POO) and 1,3-dipalmitoyl-2-oleoyl-sn-glycerol (sn-POP) into 1,3-dioleoyl-2-palmitoyl-sn-glycerol (sn-OPO) and 1,2-dipalmitoyl-3-oleoyl-sn-glycerol (sn-PPO), which increases the saturated fatty acid content at the sn-2 position. Feeding broilers with this modified oil has improved the absorption effect of saturated fatty acids and increased the content of palmitic acid in abdominal tissue by 1.55%-1.69%. The impact on the content and positional distribution of fatty acids deposited in the body is limited. Low-density lipoprotein cholesterol (LDL-C) levels decreased by 34%, while high-density lipoprotein cholesterol (HDL-C) levels increased by 23%, resulting in a lower risk of atherosclerosis. No significant differences have been observed in carcass yield results of the POL and H-AMD groups. Compared with animal-derived oils such as lard which are also rich in saturated fatty acids at the sn-2 position, plant-derived oils such as POL and its modified products have a smaller effect on abdominal fat deposition.

The Influence of Different Butter Type, Their Fatty Acid Composition and Melting Enthalpy on the Viability Rate of Lacticaseibacillusrhamnosus GG Directly After the Spray-Drying Process and During Storage of Powders.

The present work reports on the microencapsulation of Lacticaseibacillus rhamnosus GG (LGG) by the spray-drying process using a solution of starch, whey protein concentrate (WPC), soy lecithin and ascorbic acid as a carrier, with addition of different types of butters. The aim of this study was to examine the protective mechanism of six different butter samples on the viability rate of LGG bacteria directly after the spray-drying process and during storage for 4 weeks at 4 °C and 20 °C (±1 °C) based on hypothetical factors-the fatty acid's chemical character and content, and its melting enthalpy. The viability of bacteria, moisture content, water activity, color properties, morphology, particle size of powder, melting enthalpy of butters and their fatty acids composition were evaluated. It is assumed that the highest viability may be indirectly influenced by the relationship between the highest content of proteins and sugars and the lowest content of fats and fatty acids, which is characteristic for butter with a reduced fat content. This butter contained also the least monounsaturated and polyunsaturated fatty acids. The highest number of viable LGG (for systems with reduced-fat butter, as well as salted and lactose-free butter) may be caused by (among other factors) by the lower content of palmitic acid (C16: 0). For these butters, it was also observed that cell viability increased with the increase in melting enthalpy. The results confirmed the protective role of selected butters, which indicates the possibility of using them in industrial processes to increase the durability of additives and products using probiotic powders obtained by spray-drying.

Assessing the Effects of Palm Oil Consumption on Life Expectancy, Metabolic Markers, and Oxidative Stress in Drosophila melanogaster.

Palm oil is the world's second most consumed vegetable oil, sourced from the tropical palm tree Elaeis guineensis. Its consumption has been associated with a higher incidence of cardiovascular disease, largely due to its elevated palmitic acid content, however those studies are contradictory and inconclusive. Wishing to contribute to this issue, the present study aims to investigate the molecular and toxicological effects of this oil and the involvement of oxidative stress, given its role in metabolic dysfunctions using Drosophila melanogaster. This study examines survival rates, and locomotor performance, oxidative status by analysis of lipid peroxidation, ROS formation, thiol levels and antioxidant enzyme activity, and metabolic parameters such as cholesterol and triglycerides, glucose, trehalose and glycogen levels. Exposure to palm oil concentrations of 10% and 30% resulted in a shortened lifespan, reduced locomotor performance, and increased lipid peroxidation, with lower thiol levels and antioxidant enzyme modulation. Cholesterol levels was increased whereas energetic fuels as glucose and glycogen and trehalose were decreased mainly after 10 days of exposure. These findings underscore the detrimental effects of high-fat diets containing palm oil on lifespan, antioxidant defenses, and metabolism in Drosophila melanogaster. This data highlights the potential risk associated with the habitual consumption of palm oil in the daily diet by population, particularly concerning cardiovascular health and metabolic function.

Does fat identity matter? The effect of saturated and unsaturated fatty acids on bumble bee consumption and fitness

Abstract Different animal species have specific nutrient needs, guiding their foraging preferences towards particular food properties, such as specific nutrient content or ratios, and thus their ecological interactions. Bumble bees, for instance, can assess pollen fatty acid content, impacting their foraging choices and reproductive success. While they avoid high fatty acid concentrations, certain fatty acids can enhance cognitive abilities and potentially benefit bee fitness. However, the effects of specific fatty acids on bees remain unclear, even though unsaturated fatty acids are generally considered more beneficial than saturated ones. To investigate this further, we conducted feeding experiments with pollen enriched with six fatty acids at varying concentrations fed to bumble bees. Our findings reveal that bees mostly preferred pure pollen over high fatty acid concentrations. Additionally, high fatty acid concentrations negatively affected reproduction, while lower concentrations, resembling pure pollen, had no such effect. Notably, pollen enriched with stearic acid was consistently consumed regardless of concentration without adverse effects on reproduction, even at high concentrations. Conversely, low palmitic acid concentrations boosted survival and reproduction, but this benefit vanished at higher concentrations. Overall, our results emphasise the importance of fatty acid concentration for bee nutrition and fitness, while also demonstrating that individual fatty acids can have differing effects on bee behaviour and health. Pollen fatty acid composition may therefore strongly affect the bees' foraging choices and hence plant–bee interactions.

Diversity of Major Yield Traits and Nutritional Components Among Greenhouse Grown Chickpea (Cicer arietinum L.) Breeding Lines, Landraces, and Cultivars of Different Origins.

This study analyzed the diversity of major yield traits and nutritional components across 122 chickpea breeding lines, cultivars, and landraces of different origins. All parameters showed significant variations, with a variance ranging from 4.61% in days to maturity (DM) to 43.04% in oleic acid. Six accessions, including CP021, CP022, CP026, CP037, CP066, and CP109, outperformed in yield traits and nutritional value. Origin significantly affected all phenotypic traits except total fatty acid contents, with Indian and Ukrainian accessions demonstrating contrasting performances. Most traits, except for the number of seeds per pod (SPP), palmitic acid, and total fatty acid contents, differed significantly among breeding lines, cultivars, and landraces. Breeding lines were the earliest to flower and to mature with average days to flowering (DF) of 50.23 days and DM of 101.50 days. They also had the highest average SPP, number of pods per plant (PPP), total seeds per plant (TSPP), total protein, crude fiber, dietary fiber, linoleic acid, and linolenic acid contents making them preferable for high yield and nutrition. Hierarchical cluster analysis classified the chickpea accessions into seven clusters, showing significant variations in yield traits and nutritional components. Principal component and Pearson's correlation analyses indicated positive correlations between DM and DF, and between SPP, PPP, and TSPP. Nutritional components also displayed varying associations, with a notable negative correlation between oleic and linoleic acids, the two essential fatty acids. Overall, this study showed the diversity of key phenotypic traits in chickpea breeding lines, cultivars, and landraces of different origins. The significant effects of genotype and origin differences on these traits could be used as a basis for future metabolomics and genomics research.

Genome- and transcriptome-wide association studies reveal the genetic basis of seed palmitic acid content in Brassica napus

Rapeseed (Brassica napus L.) is one of the most important oilseed crops worldwide. Development of rapeseed varieties with high-quality oil is a long-term breeding goal. Reducing the contents of palmitic acid, the main saturated fatty acid in rapeseed oil, could greatly improve oil quality. Here, we performed genome-wide association study (GWAS) and transcriptome-wide association study (TWAS) of seed palmitic acid content (SPAC) using 393 diverse B. napus accessions. Four genes (BnaA08.DAP, BnaA08.PAA1, BnaA08. DUF106, and BnaC03.DAP) were identified by both GWAS and TWAS. The transcripts per million (TPM) values of these candidate genes at 20 and 40 days after flowering (DAF) were significantly correlated with SPAC in this association panel. Based on genetic variation in the candidate genes, we identified four low-SPAC haplotypes by combining candidate gene association analysis and haplotype analysis. Brassica napus accessions carrying low-SPAC haplotypes had lower SPAC than those carrying high-SPAC haplotypes without affecting seed oil content, seed protein content, or seed yield. Based on the functional single-nucleotide polymorphism (SNP) chrA08_9529850 (C/A) in the promoter of BnaA08.DUF106, we developed a molecular marker (Bn_A8_SPAC_Marker) that could be used to facilitate breeding for low SPAC in B. napus. Our findings provide valuable information for studying the genetic control of SPAC in B. napus. Moreover, the candidate genes, favorable haplotypes, and molecular marker identified in this study will be useful for breeding low-SPAC B. napus varieties.

Impact of freeze drying on the properties of palmitic acid extracted from Plantain stalk waste

The challenge of effectively preserving and concentrating palmitic acid from plantain stalk waste is compounded by the loss and degradation of the compound during traditional drying and extraction methods, which may lead to reduced yield and altered properties of the extracted palmitic acid. This study was aimed at evaluating the impact of the freeze-drying process on the properties of palmitic acid extracted from plantain stalk waste. The study involved preparing plantain stalks, applying freeze-drying or non-freeze-drying methods, extracting palmitic acid, and characterizing the samples using GC-MS, FTIR, XRD, EDX, and SEM. The GC-MS analysis revealed that freeze-drying significantly increased the concentration of palmitic acid compared to non-freeze-drying, as indicated by the higher peak area and height. FTIR spectra demonstrated that freeze-drying better preserved the structural integrity of palmitic acid, while XRD analysis showed enhanced crystalline structure and increased crystallite size in the freeze-dried sample. Additionally, EDX showed that freeze-drying increased the carbon content and decreased the oxygen content of the material, while SEM analyses indicated that freeze-drying resulted in a more porous and irregular surface compared to the denser, smoother structure of the non-freeze-dried sample. The mass, energy, and cost analysis revealed that freeze-drying resulted in a higher yield of palmitic acid compared to non-freeze-drying methods, which, despite being more cost-effective and requiring less energy, demonstrated a notable trade-off in extraction efficiency. The study showed that freeze-drying significantly improved the preservation, concentration, and structural integrity of palmitic acid extracted from plantain stalk waste compared to the non-freeze-drying method.

Fatty Acid Profiling of Major Fatty Acids in Safflower Genotype

Fatty acid profiling is a process of analyzing the composition and quantity of different fatty acids. Fatty acids are organic molecules that are essential for a variety of biological processes in the body, including energy storage and membrane function. Safflower seed contains major four fatty acids viz. linoleic acid, Oleic acid, palmitic acid and stearic acid. The analysis of fatty acid profiles is important for understanding the nutritional quality of the oil and products derived from it. According to hull types of seeds, the seed oil content ranges from 20 % to 45 %. Fatty acid profiling of safflower involves analyzing the types and quantities of fatty acids present in safflower oil. This can provide insights into its nutritional value, stability, and potential health benefits from the oil derived from each genotype. This research is conducted to know the content of saturated as well as unsaturated fatty acid in safflower oil from selected genotypes. The major four fatty acid composition of safflower genotypes reveals palmitic acid content ranges from 5.09% (GMU-2969) to 6.93% (GMU-3929). The stearic acid content ranged between 1.69% (GMU-2969) to 3.55% (GMU-3780). The oleic acid content ranged from 12.88% (GMU-2749) to 20.69% (GMI-3177) and the percent linoleic acid content ranged from 71.31 (GMU-3177) to 78.44% (GMU-2749).

Seed inoculation with Rhizobium japonium bacteria improved fatty acid composition of different soybean (Glycine max L.) genotypes

BackgroundSoybean is an important crop for food security as it fulfills global oil requirements. Seed inoculation with bacteria is frequently used to increase its production; however, it could change the seed composition. Nevertheless, the genotypes respond differently to the bacteria. Therefore, it is necessary to assess the impact of Rhizobium bacteria on the seed composition. MethodsThis two-year (2018 and 2019) study investigated the effects of seed inoculation with Rhizobium japonicum on fatty acid composition of different soybean genotypes. Three frequently cultivated soybean genotypes, i.e., ‘Gapsoy16′, ‘Traksoy’, and ‘İlksoy’ were included in the study. The seeds were either inoculated with R. japonicum or sown without inoculation. The fatty acid profile, i.e., saturated fatty acids (palmitic and stearic acid) and unsaturated fatty acids (oleic, linoleic, linolenic, and arachidic acids) was determined, and the collected data were analyzed by single and multivariate analysis. ResultsSeed inoculation with R. japonicum significantly altered the fatty acid composition of different genotypes; however, varied effects were recorded for the genotype. Linoleic acid, oleic acid, and palmitic acid made up ∼ 33 % of total fatty acids in seeds. Linoleic acid contents varied between 30.78–34.02 %, whereas oleic acid contents ranged between 27.85–31.04 %. Similarly, palmitic acid contents differed between 15.53–16.93 %. The ‘İlksoy’ and ‘Gapsoy’ had the highest contents of palmitic and oleic acids, respectively. Overall, inoculation of bacteria increased the composition of unsaturated fatty acids and lowered saturated fatty acids. ConclusionSeed inoculation with R. japonicum increased the essential fatty acid composition in ‘Traksoy’ genotype. However, ‘İlksoy’ genotype recorded a decrease in unsaturated fatty acids. Therefore, ‘Traksoy’ can be inoculated with R. japonicum to improve fatty acid profile.

Exploring the use of POLY4 for the improvement of productivity, peanut quality, and soil properties in Southern India.

Polyhalite-based POLY4, a multi-nutrient source containing potassium, calcium, magnesium, and sulphur, is increasingly recognised for its potential to improve crop yields and soil health in agricultural systems. It is also been considered as a feasible approach for addressing the deficiency in potassium, calcium, and sulphur within a single application source. The present study aimed to investigate the impact of polyhalite-based POLY4 application, either as a complete or partial substitute for traditional potassium fertiliser and gypsum supplement, on the improvement of peanut (Arachis hypogaea) growth and soil quality. An extensive field study was conducted from 2021 and 2022, employing ten distinct nutrient management treatments with three replications in a randomised complete block design. The findings of the study indicated that the application of polyhalite (POLY4) in conjunction with only NP fertilisers resulted in a higher yield advantage (approximately 150-200 kg ha-1) than in plots treated with NPK + gypsum (at 500 kg ha-1) and control plots. The application of polyhalite-based fertiliser (POLY4) at a rate that was 100% equivalent to K along with NP fertilisers resulted in a significant increase in pod yield (5.3-12.8%) over NPK + gypsum and control plots. Thus, the increased crop yield led to an increase in gross returns of 4.88% and in net returns of 4.28% with the application of POLY4 (100%) + NP fertilisers over other treatments. Likewise, variable rates of conventional fertilisers along with POLY4 (100% recommended) + NP + gypsum at 310 kg/ha significantly increased the linoleic acid content (38.5%), oleic acid content, and oil content (48.1%) by reducing palmitic acid (11.96%) content in the groundnut seed. Interestingly, POLY4 use at the 50% recommended rate also resulted in yields that were comparable with those obtained with 100% NPK. Therefore, applying POLY4, a polyhalite fertiliser, in either a 100% or 50% equivalent of essential K can be an effective way of increasing the production of peanut crops and promoting agricultural sustainability.

Associations of the rs12504538 and rs6824447 Polymorphisms of the Elovl6 Gene with Estimated Elongase and Desaturase Activity and Fatty Acid Concentrations in Mexican Women with Gestational Diabetes Mellitus

Background: This study aimed to investigate the possible associations of the rs12504538 and rs6824447 polymorphisms of the Elovl6 gene with estimated elongase and desaturase activity and saturated fatty acid concentrations in Mexican women with Gestational Diabetes Mellitus (GDM). Methods: We recruited 172 women in the second and third trimesters of pregnancy who had undergone an oral glucose tolerance test, including 66 who had diagnosed with GDM according to the 2016 ADA criteria and 106 who had normal glucose tolerance test results, from the General Hospital and Health Centers of the Health Ministry of Guanajuato, México. Participants were matched by gestational week. Data on age, gestational week and anthropometric characteristics were collected. Blood samples were drawn after an overnight fast for the measurement of serum glucose, lipid, NEFA, serum fatty acid and insulin levels; SNP genotyping and quantification of fatty acids was performed and elongase and desaturase activity was estimated. Findings: With the exception of HDL-cholesterol, all variables, including NEFA levels, were significantly greater in the GDM group than in the non-GDM group. The rs6824447 polymorphism of the Elovl6 gene, an age &gt;25 years and HOMA-IR levels were associated with the development of GDM (OR=5.1, 95% CI 1.56-17.1, p=0.006; OR=4.89, 95% CI 1.65-14.4, p=0.003; OR=34.1 95% CI 8.7-133, p&lt;0.000001, respectively). Palmitic acid (OR=1.08; 95% CI: 1.06-1.11; p&lt;0.001) and oleic acid (OR=1.17; 95% CI: 1.14-1.2; p&lt;0.001) concentrations were also associated with the development of GDM. No differences in the estimated elongase and desaturase activity among the non-GDM and GDM groups were found and only the activity of Δ9D SCD18 desaturase was marginally high in GDM patients (p=0.050). Conclusion: The rs6824447Elovl6 polymorphism is associated with the development of GDM, as are high serum palmitic acid, oleic acid and stearic acid concentrations and estimated Δ9D SCD18 desaturase activity.

Genetic and environmental influences on fatty acid composition in different fenugreek genotypes

Fenugreek (Trigonella foenum-graecum L.) plays a crucial role in both the food and pharmaceutical industries due to its rich nutritional profile and medicinal properties, such as regulating blood sugar and reducing inflammation. This study offers a comprehensive analysis of the fatty acid composition in fenugreek seeds, revealing significant variability influenced by genetic and environmental factors. The analysis showed that linoleic acid ranged from 32.16 % to 45.96 %, linolenic acid from 17.3 % to 31.21 %, oleic acid from 11.40 % to 20.32 %, stearic acid from 3.78 % to 6.76 %, and palmitic acid from 8.74 % to 16.44 %. Both genotype and environmental conditions had a profound impact on these profiles, with notable variations arising from differences in water availability. The Israel and Kermanshah (Iran) genotypes recorded the highest linoleic acid levels under irrigated conditions, while the Kayseri (Turkey) genotype exhibited the highest linolenic and stearic acid values across both irrigated and non-irrigated environments. Additionally, the Ahvaz (Iran) and Ukraine genotypes excelled in oleic and palmitic acid concentrations. Principal component analysis (PCA) and Additive Main Effects and Multiplicative Interaction (AMMI) biplots further highlighted the complex genotype-environment interactions, providing essential insights for breeding fenugreek varieties optimized for diverse agro-climatic conditions.

Comparative Study on Growth and Metabolomic Profiles of Six Lactobacilli Strains by Sodium Selenite

Selenium (Se) has garnered increasing attention in the field of nutrition, as it is essential for both humans and animals. Certain microorganisms can enrich inorganic selenium and convert it into organic selenium. The growth and metabolomic profiles of six lactobacilli strains exposed to 50 μg/mL of sodium selenite were performed using gas chromatography tandem time-off light mass spectrometry (GC-TOF-MS) analysis. The addition of selenium significantly increased both the population and weight of the Lacticaseibacillus rhamnosus PS5, Lbs. rhamnosus RT-B, Limosilactobacillus reuteri 3630, and Lmb. reuteri 1663 strains, while those of the other two strains decreased. A total of 271 metabolites were determined, with their concentrations ranked from highest to lowest as follows: organic acids and derivatives, oxygen compounds, lipids and lipid-like molecules, and benzenoids. In certain groups, the concentrations of serine, aspartic acid, trehalose, palmitic acid, methylthreonine, and melibiose increased significantly, whereas glucuronic acid, ribose, ornithine, and methionine were downregulated. The metabolic pathways were significantly associated with ABC transporters, glycine, serine, threonine metabolism, and aminobenzoate degradation and other pathways. Based on these findings, we concluded that the transport, absorption, assimilation, and stress response to selenium by lactobacilli in metabolomic changed. Furthermore, the metabolomic alterations among different types of lactobacilli varied primarily due to their distinct properties.

Fatty acid composition and sensory properties as descriptors of differentiation of specialty coffees based on spontaneous and induced processing methods

The composition of fatty acids (FA) and sensory properties of specialty coffees from Guatemala, Nicaragua, Peru, Ethiopia, Kenya, Burundi, and Rwanda processed by spontaneous methods (i.e., natural, washed, and honey) and induced methods (i.e., anaerobic fermentation and carbonic maceration) were determined. Palmitic, stearic, oleic, linoleic, linolenic, and arachidic acids and 10 important attributes of the coffee such as fragrance/aroma, flavour, aftertaste, acidity, body, balance, overall, uniformity, clean cup, sweetness, and total score were evaluated. The FA composition and sensory scores following the Specialty Coffee American Association protocols were used to differentiate 19 coffee samples based on the processing method. Principal component analysis (PCA) effectively differentiated coffees processed by spontaneous methods from those processed by induced methods. Notably, Peruvian coffee processed by carbonic maceration exhibited the highest linoleic acid content, while the same coffee processed naturally showed the highest palmitic acid content. All coffee samples scored 10 points for uniformity, clean cup, and sweetness. The highest total scores for the spontaneous processing methods were 88.75 (washed Kenyan) and 87.75 (washed Ethiopian) but ranged from 82.75 to 85.00 for the other coffees. For the best specialty coffees processed by induced methods, scores ranged from 87.25 to 88.00 in seven out of nine coffees. Coffees from different geographical areas processed with innovative methods, such as anaerobic fermentation, carbonic maceration, and high-scoring spontaneously processed coffees, were among the highest qualities.

SiO2-FATTY ACIDS AND TiO2-FATTY ACIDS WITH THE ROLE OF BASED HYDROPHOBIC COATINGS FOR THE PRESERVATION OF ANDESITE STONE

This study explains the innovation of self-cleaning material and preservation agent for andesite stone by consecutively layering the surface with SiO2 and fatty acid through dip coating method. SiO2 and TiO2 behaved as surface roughness agent, while palmitic acid and stearic acid were functioned to create hydrophobic layer by lowering the surface tension. The performance of the surface was defined as the hydrophobicity indicated by the contact angle formed by the water droplet. The influence of dip coating parameters was investigated including SiO2 or TiO2 and stearic acid or palmitic acid concentration, contact time, drying temperature as well as pH of hydrolysis. The highest contact angles were obtained by TiO2-stearic acid coatings with the water contact angle about 136.6±0.9ᵒ. These maximum values were obtained over dip coating condition as follow: TiO2 0.04 M, stearic acid 0.16 M, 30 minutes of contact time, 95 ᵒC of drying temperature and acid hydrolysis.

Palmitic acid promotes miRNA release from adipocyte exosomes by activating NF-κB/ER stress

ObjectiveThe release of adipose tissue-derived miRNAs is increased under conditions of obesity, but the exact molecular mechanisms involved have not been elucidated. This study investigated whether obesity-induced increases in palmitic acid (PA) content could activate the NF-κB/endoplasmic reticulum stress (ER stress) pathway and promote the expression and release of exosomal miRNAs in adipocytes.MethodsAbdominal adipose tissue and serum samples were collected from normal weight individuals and people with obesity to clarify the correlation of serum PA content with NF-κB/ER stress and the release of exosomal miRNAs. NF-κB and ER stress were blocked in obese mice and in vitro cultured adipocytes to demonstrate the molecular mechanisms by which PA promotes the release of exosomal miRNAs.The morphology, particle size and distribution of the exosomes were observed via transmission electron microscopy and NTA.ResultsAccompanied by increased serum PA levels, the NF-κB/ER stress pathway was activated in the adipose tissue of people with obesity and in high-fat diet (HFD)-induced obese mice; moreover, the levels of miRNAs in both adipose tissue and serum were increased. P-p65 (Bay11-7082) and ER stress (TUDCA) blockers significantly reduced the levels of miRNAs in abdominal adipose tissue and serum, decreased blood glucose levels, and improved glucose tolerance and insulin sensitivity in obese mice. In 3T3-L1 adipocytes, high concentrations of PA activated the NF-κB/ER stress pathway and increased the expression and release of miRNAs in exosomes. P-p65 (Bay11-7082) and ER stress (TUDCA) blockers significantly reversed the increased release exosomal miRNAs cause by PA.ConclusionsObesity-induced increases in PA content increase the expression and release of miRNAs in adipocyte exosomes by activating the NF-κB/ER stress pathway.

Low temperature conditioning and nitric oxide treatment reduced chilling injury and altered fatty acid metabolism of flat peaches

Low-temperature conditioning is a recognized method for alleviating chilling injuries in flat peaches. However, long-term low-temperature storage can mitigate volatile organic compounds, thereby limiting fruit availability. To improve the supply of flat peaches, we investigated the combined effects of low-temperature conditioning and nitric oxide treatment on various parameters including physiological quality, volatile organic compounds, fatty acids, associated enzymes, and genes involved in the fatty acid pathway with the transcriptome sequencing analysis. The results indicated that low-temperature conditioning coordinated with 10 µL L−1 nitric oxide treatment possessed higher firmness and vitamin C content and restricted the content of malonaldehyde and relative electrical conductivity, thus maintaining the overall quality of flat peaches. In the study, 10 µL L−1 nitric oxide was employed for subsequent experiments. Moreover, higher contents of volatile organic compounds were supported by increased contents of palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, alcohol dehydrogenase, hydroperoxide lyase, and up-regulated genes of LOX2, LOX3, LOX6, ADH2, and ADH3. In summary, nitric oxide treatment effectively facilitated the recovery of volatile organic compounds in flat peaches during cold storage. Therefore, low-temperature conditioning and nitric oxide treatment can be a promising strategy to prevent the loss of volatile organic compounds by modulating the fatty acid pathway and associated genes.