Three strictly endogamous groups of Indian, Brahmin, Bhangi, and Punjabi Khattri, were analyzed to test the authenticity of palmar flexion creases as a criterion of human classification, according to the method of Bali and Chaube ('71), which has certain advantages over the existing classification. The significant frequency variation of palmar creases among different Indian populations and the bisexual and bimanual differences in the frequencies of different crease types indicate a regular distribution trend of the trait.